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The map label for this gene is gap [H]

Identifier: 39996729

GI number: 39996729

Start: 1784444

End: 1785445

Strand: Reverse

Name: gap [H]

Synonym: GSU1629

Alternate gene names: 39996729

Gene position: 1785445-1784444 (Counterclockwise)

Preceding gene: 39996742

Following gene: 39996728

Centisome position: 46.81

GC content: 57.49

Gene sequence:

>1002_bases
ATGGCTCTCAGAGTTGCAATCAACGGTTTCGGCAGGATCGGACGCTCCGTGCTCCGCGCGGCTGCCAAGGAGAAGGGCAT
TGAGATTGTTGCCATCAACGACCTCACCGACGCCAAGACCCTGGCTCACCTTCTTAAATACGATTCGGTCCACGGTCCGT
TCCCCGGCAAGGTTGAGGTAGGCGACGGCGCAATCGTCGTGAACGGCAAGCCCATCACCATCATCGCCGAGAGAAATCCC
GAACTTCTCCCGTGGAAAAAGATGAAGATCGACGTGGTCCTGGAAGCGACGGGCCTGTTCACGGCACGTGAAAAAGCCGA
ACTCCACCTGAAGGCTGGCGCCAAGAAAGTCATCATCTCGGCTCCCGCCACCAACGAAGACATCACCATCGTCATGGGGG
TCAACCACGACGCCTATGATCCGAAGAAGCACAACATCATCTCAAACGCATCCTGCACCACAAACTGCCTCGCCCCCGTG
GCAAAGGTTCTCCATGAAACCTTCGGCATCGAAAAAGGATTGGTTACCACGGTTCACTCCTACACCAACGACCAGCAGAT
TCTCGACCTTCCCCACAAGGACCTGCGTCGCGCCCGGGCAGCGGCCATGTCCATGATCCCGACCACCACCGGTGCCGCCA
AAGCGGTCTCCCTCGTTCTTCCGGAACTGAAAGGGAAACTGGACGGCATGGCCATCCGCGTCCCGACCCCCAACGTCTCG
GTGGTCGACCTCGTGGTCACCCTCAAGAAGAAGACCGATGCCGAAAAAGTGAACGCAGCCCTCAAGAAAGCCGCCAAAGG
GAGCCTCAAGGGAATCCTCAGGTTCGAGGAAGAGCCCCTGGTCTCCATCGACTTTAACGGCACCACCCACTCCTCCATCG
TTGACGCTCTGAGCACCAAGGTCATTGACGGCACCATGGTAAAGGTTCTTTCGTGGTATGACAACGAAACCGGTTTCTCC
AACCGGGTCGTGGACCTGATGAAGCTCATCGCGTCCAAGTAA

Upstream 100 bases:

>100_bases
GGCACAAAACGGTTGACGCTCGGCCCGCAACTCTATATGATTGTGAGGTTTGTATACTAAAGGCTCGCCGAGCTCTCACA
TCACGCGACAGGAGGATTCA

Downstream 100 bases:

>100_bases
ACCGGATACGCGCAAAAGGGGGGATCAATCCCCCCTTTTCTTTTTGCCCGCCAGTCGAACCGATCCCGCCCCTGCATTGC
TGACACTATCTACTCGGGAG

Product: glyceraldehyde 3-phosphate dehydrogenase 1

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MALRVAINGFGRIGRSVLRAAAKEKGIEIVAINDLTDAKTLAHLLKYDSVHGPFPGKVEVGDGAIVVNGKPITIIAERNP
ELLPWKKMKIDVVLEATGLFTAREKAELHLKAGAKKVIISAPATNEDITIVMGVNHDAYDPKKHNIISNASCTTNCLAPV
AKVLHETFGIEKGLVTTVHSYTNDQQILDLPHKDLRRARAAAMSMIPTTTGAAKAVSLVLPELKGKLDGMAIRVPTPNVS
VVDLVVTLKKKTDAEKVNAALKKAAKGSLKGILRFEEEPLVSIDFNGTTHSSIVDALSTKVIDGTMVKVLSWYDNETGFS
NRVVDLMKLIASK

Sequences:

>Translated_333_residues
MALRVAINGFGRIGRSVLRAAAKEKGIEIVAINDLTDAKTLAHLLKYDSVHGPFPGKVEVGDGAIVVNGKPITIIAERNP
ELLPWKKMKIDVVLEATGLFTAREKAELHLKAGAKKVIISAPATNEDITIVMGVNHDAYDPKKHNIISNASCTTNCLAPV
AKVLHETFGIEKGLVTTVHSYTNDQQILDLPHKDLRRARAAAMSMIPTTTGAAKAVSLVLPELKGKLDGMAIRVPTPNVS
VVDLVVTLKKKTDAEKVNAALKKAAKGSLKGILRFEEEPLVSIDFNGTTHSSIVDALSTKVIDGTMVKVLSWYDNETGFS
NRVVDLMKLIASK
>Mature_332_residues
ALRVAINGFGRIGRSVLRAAAKEKGIEIVAINDLTDAKTLAHLLKYDSVHGPFPGKVEVGDGAIVVNGKPITIIAERNPE
LLPWKKMKIDVVLEATGLFTAREKAELHLKAGAKKVIISAPATNEDITIVMGVNHDAYDPKKHNIISNASCTTNCLAPVA
KVLHETFGIEKGLVTTVHSYTNDQQILDLPHKDLRRARAAAMSMIPTTTGAAKAVSLVLPELKGKLDGMAIRVPTPNVSV
VDLVVTLKKKTDAEKVNAALKKAAKGSLKGILRFEEEPLVSIDFNGTTHSSIVDALSTKVIDGTMVKVLSWYDNETGFSN
RVVDLMKLIASK

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=333, Percent_Identity=52.5525525525526, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI7657116, Length=333, Percent_Identity=50.4504504504504, Blast_Score=337, Evalue=8e-93,
Organism=Escherichia coli, GI1788079, Length=332, Percent_Identity=55.1204819277108, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1789295, Length=329, Percent_Identity=48.3282674772036, Blast_Score=328, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI17534677, Length=337, Percent_Identity=50.4451038575668, Blast_Score=322, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17534679, Length=337, Percent_Identity=50.4451038575668, Blast_Score=321, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI32566163, Length=336, Percent_Identity=50.8928571428571, Blast_Score=314, Evalue=4e-86,
Organism=Caenorhabditis elegans, GI17568413, Length=336, Percent_Identity=50.8928571428571, Blast_Score=314, Evalue=4e-86,
Organism=Saccharomyces cerevisiae, GI6322409, Length=331, Percent_Identity=52.2658610271903, Blast_Score=340, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6322468, Length=331, Percent_Identity=51.0574018126888, Blast_Score=330, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6321631, Length=331, Percent_Identity=51.0574018126888, Blast_Score=329, Evalue=3e-91,
Organism=Drosophila melanogaster, GI85725000, Length=332, Percent_Identity=52.1084337349398, Blast_Score=331, Evalue=4e-91,
Organism=Drosophila melanogaster, GI22023983, Length=332, Percent_Identity=52.1084337349398, Blast_Score=331, Evalue=4e-91,
Organism=Drosophila melanogaster, GI17933600, Length=332, Percent_Identity=51.8072289156626, Blast_Score=330, Evalue=6e-91,
Organism=Drosophila melanogaster, GI18110149, Length=332, Percent_Identity=51.8072289156626, Blast_Score=330, Evalue=6e-91,
Organism=Drosophila melanogaster, GI19922412, Length=325, Percent_Identity=48.9230769230769, Blast_Score=306, Evalue=9e-84,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 35819; Mature: 35688

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRVAINGFGRIGRSVLRAAAKEKGIEIVAINDLTDAKTLAHLLKYDSVHGPFPGKVEV
CEEEEEECCHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEE
GDGAIVVNGKPITIIAERNPELLPWKKMKIDVVLEATGLFTAREKAELHLKAGAKKVIIS
CCCEEEECCCEEEEEECCCCCCCCCCCEEEEEEEEECCCEEECCCCEEEEECCCEEEEEE
APATNEDITIVMGVNHDAYDPKKHNIISNASCTTNCLAPVAKVLHETFGIEKGLVTTVHS
CCCCCCCEEEEEECCCCCCCCHHCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHEEHH
YTNDQQILDLPHKDLRRARAAAMSMIPTTTGAAKAVSLVLPELKGKLDGMAIRVPTPNVS
CCCCCCEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCE
VVDLVVTLKKKTDAEKVNAALKKAAKGSLKGILRFEEEPLVSIDFNGTTHSSIVDALSTK
EEEEEEEHHCCCCHHHHHHHHHHHHCCCHHEEEEECCCCEEEEEECCCCHHHHHHHHHHH
VIDGTMVKVLSWYDNETGFSNRVVDLMKLIASK
HCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALRVAINGFGRIGRSVLRAAAKEKGIEIVAINDLTDAKTLAHLLKYDSVHGPFPGKVEV
EEEEEECCHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEE
GDGAIVVNGKPITIIAERNPELLPWKKMKIDVVLEATGLFTAREKAELHLKAGAKKVIIS
CCCEEEECCCEEEEEECCCCCCCCCCCEEEEEEEEECCCEEECCCCEEEEECCCEEEEEE
APATNEDITIVMGVNHDAYDPKKHNIISNASCTTNCLAPVAKVLHETFGIEKGLVTTVHS
CCCCCCCEEEEEECCCCCCCCHHCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHEEHH
YTNDQQILDLPHKDLRRARAAAMSMIPTTTGAAKAVSLVLPELKGKLDGMAIRVPTPNVS
CCCCCCEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCE
VVDLVVTLKKKTDAEKVNAALKKAAKGSLKGILRFEEEPLVSIDFNGTTHSSIVDALSTK
EEEEEEEHHCCCCHHHHHHHHHHHHCCCHHEEEEECCCCEEEEEECCCCHHHHHHHHHHH
VIDGTMVKVLSWYDNETGFSNRVVDLMKLIASK
HCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2684782; 2227448; 2656407; 7408868; 193030; 3586018; 9175858 [H]