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The map label for this gene is pgk [H]

Identifier: 39996728

GI number: 39996728

Start: 1782373

End: 1784337

Strand: Reverse

Name: pgk [H]

Synonym: GSU1628

Alternate gene names: 39996728

Gene position: 1784337-1782373 (Counterclockwise)

Preceding gene: 39996729

Following gene: 39996727

Centisome position: 46.78

GC content: 57.71

Gene sequence:

>1965_bases
ATGGCAATCCGCTATATTGACGAAATATCCGAACTCAAAGACAAAAAGGTCTTCATCAGGGTCGACTTCAACGTTCCCTT
GGATGAACATCAGAACATCACTGAAGACACGCGCATCAGGGCGGTCCTCCCTACGATCAACTTTGCCCTCGATGCCGGCG
CGAAAGTGGTTCTCGCCTCTCACCTGGGACGCCCAAAGGGAGAGCGCAAGCCCAAGTATTCCATGGCCCCGGCGGCAAAG
CGTCTCTCGCGGCTTCTGGGCAAAGAGGTACTCTTGGCGCCCGACTGCATCGGCAGCGAAGTACGGCAGATGATCGATGC
CATGAAATCCGGCGACATCATCATGCTGGAAAACGTGCGCTTTTATGAAGGTGAGGAGAAAAATGACGAAAACTTTGCCC
GAGAACTGGCCAACGGCTGTGAGATCTACGTAAACGATGCCTTTGCCGTCTCACACCGGGCTCATGCGTCGGTTGAAGCG
ATAACAAAGTTCTTTCCGGTAGTTGCGGCCGGATTTCTCATGAAGAACGAGATGAATTATTTCGAAAAGGCGATGCAAAA
GCCGATTCGCCCCCTGGTCGCTATCTTGGGCGGGGCCAAAGTCTCGGGCAAACTGGAAGTTCTCGAAAGCCTTCTCAACA
AGGTGGACAAAATCATCATCGGCGGCGGCATGGCATTCACCTTTCTGAAGGCCCTCGGCTACAACGTGGGCAAGTCGCTG
GTGGAGGAAGACCTGATCGAAACGGCCCGCTCAACCTACGCCAAAGCCCGTGAAAAGGGAATCAAGTTCTATCTCCCCGT
TGATTGCGTGGCTGCCGATCGATTCAACCCCGAGGCCGAGACCAAGGTAACCACCATTCAGGAAATCCCCGAAGAGTGGA
TGGCTCTCGATATCGGTCCTGCCACGGTTGCCCTCTTCACCGAGGCACTCCAGAGCGCCAAGACCATCATCTGGAACGGT
CCCATGGGGGTTTTCGAGATGGATGCCTTCTCCCGCGGCACCTTTGCCATGGTGACTGCCGTGGCCAACTCCTACGCCCT
TACCATCGTCGGCGGCGGCGACACCGACTCTGCGGTCCACCGCGCCGGCGAATATGCCAAGATCAGCTACATCTCCACCG
GCGGCGGCGCTTTCCTTGAGCTCCTCGAAGGCAAGAAACTTCCCCGGCATCAAGGTGCTGGAAGAAAACGGAAAATAGGA
GGAGCGGCCATGAGAACACCTGTGATTGCAGGAAACTGGAAGCTGTTCAAAAAACGCGCCGAGGCCCGTGAGCTGGTCAA
TGGACTTGTTTCCCTGGTGAAGGACGTCCAGGGGGTCGAAATCGTCGTAGCTCCGGTCTTCACCGTACTGGCCGCCGTAA
AGGGCGCCCTGGACGGCTCAGCCATCAAGCTCGCTGGCCAGGACTGCTTCTGGGAAGAGGAAGGGGCTTACACCGGCGAG
ATTTCCCCGGGCATGCTCGTGGATGCCGGATGCAGCCACGTCATTATCGGCCATTCGGAGCGCCGTCAGTATTTCGGCGA
AACTGATGAAACCGTGAATCGTAAGATCAAGGCCGCCCTCAAAGCAGGACTCGCGGCCATTGCCTGCATCGGAGAATCCC
TGGCAGAGCGCGAAGCAGGCACGACGTTCGACGTGCTGCGGACGCAGCTGAACGGCGGACTGGCCGGGCTATCCGCCGAC
GACCTCAAAGACGTCATCATCGCCTATGAGCCGGTATGGGCCATAGGCACCGGAAAAACCGCCACGGACGCCCAAGCCCA
GGAGGCTCACGCCTTCATCAGAGGGGTGGTGTCCGAACTTCTCGGCAAACCGGCAGCAGAGGCGGTTCGCATCCTTTACG
GGGGCAGCGTAAAGCCAGAGAACATCAAGGGGCTCATGACCCAACCCGATATCGACGGCGCCCTGGTGGGAGGAGCCAGC
CTCAAGGCGGACTCTTTTGCGGCGATTGCCTCGTTCAGCATGTAA

Upstream 100 bases:

>100_bases
TACGCGCAAAAGGGGGGATCAATCCCCCCTTTTCTTTTTGCCCGCCAGTCGAACCGATCCCGCCCCTGCATTGCTGACAC
TATCTACTCGGGAGGAAACC

Downstream 100 bases:

>100_bases
ACACGAAAGAACGCCAAGTTAGGACTTGGCTTTTCAACTAACCTGTGGTATGGATTGCAGGTTACTACATACAGTGGAGT
TGGCATTATGATGATCTTTT

Product: bifunctional phosphoglycerate kinase/triosephosphate isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 654; Mature: 653

Protein sequence:

>654_residues
MAIRYIDEISELKDKKVFIRVDFNVPLDEHQNITEDTRIRAVLPTINFALDAGAKVVLASHLGRPKGERKPKYSMAPAAK
RLSRLLGKEVLLAPDCIGSEVRQMIDAMKSGDIIMLENVRFYEGEEKNDENFARELANGCEIYVNDAFAVSHRAHASVEA
ITKFFPVVAAGFLMKNEMNYFEKAMQKPIRPLVAILGGAKVSGKLEVLESLLNKVDKIIIGGGMAFTFLKALGYNVGKSL
VEEDLIETARSTYAKAREKGIKFYLPVDCVAADRFNPEAETKVTTIQEIPEEWMALDIGPATVALFTEALQSAKTIIWNG
PMGVFEMDAFSRGTFAMVTAVANSYALTIVGGGDTDSAVHRAGEYAKISYISTGGGAFLELLEGKKLPRHQGAGRKRKIG
GAAMRTPVIAGNWKLFKKRAEARELVNGLVSLVKDVQGVEIVVAPVFTVLAAVKGALDGSAIKLAGQDCFWEEEGAYTGE
ISPGMLVDAGCSHVIIGHSERRQYFGETDETVNRKIKAALKAGLAAIACIGESLAEREAGTTFDVLRTQLNGGLAGLSAD
DLKDVIIAYEPVWAIGTGKTATDAQAQEAHAFIRGVVSELLGKPAAEAVRILYGGSVKPENIKGLMTQPDIDGALVGGAS
LKADSFAAIASFSM

Sequences:

>Translated_654_residues
MAIRYIDEISELKDKKVFIRVDFNVPLDEHQNITEDTRIRAVLPTINFALDAGAKVVLASHLGRPKGERKPKYSMAPAAK
RLSRLLGKEVLLAPDCIGSEVRQMIDAMKSGDIIMLENVRFYEGEEKNDENFARELANGCEIYVNDAFAVSHRAHASVEA
ITKFFPVVAAGFLMKNEMNYFEKAMQKPIRPLVAILGGAKVSGKLEVLESLLNKVDKIIIGGGMAFTFLKALGYNVGKSL
VEEDLIETARSTYAKAREKGIKFYLPVDCVAADRFNPEAETKVTTIQEIPEEWMALDIGPATVALFTEALQSAKTIIWNG
PMGVFEMDAFSRGTFAMVTAVANSYALTIVGGGDTDSAVHRAGEYAKISYISTGGGAFLELLEGKKLPRHQGAGRKRKIG
GAAMRTPVIAGNWKLFKKRAEARELVNGLVSLVKDVQGVEIVVAPVFTVLAAVKGALDGSAIKLAGQDCFWEEEGAYTGE
ISPGMLVDAGCSHVIIGHSERRQYFGETDETVNRKIKAALKAGLAAIACIGESLAEREAGTTFDVLRTQLNGGLAGLSAD
DLKDVIIAYEPVWAIGTGKTATDAQAQEAHAFIRGVVSELLGKPAAEAVRILYGGSVKPENIKGLMTQPDIDGALVGGAS
LKADSFAAIASFSM
>Mature_653_residues
AIRYIDEISELKDKKVFIRVDFNVPLDEHQNITEDTRIRAVLPTINFALDAGAKVVLASHLGRPKGERKPKYSMAPAAKR
LSRLLGKEVLLAPDCIGSEVRQMIDAMKSGDIIMLENVRFYEGEEKNDENFARELANGCEIYVNDAFAVSHRAHASVEAI
TKFFPVVAAGFLMKNEMNYFEKAMQKPIRPLVAILGGAKVSGKLEVLESLLNKVDKIIIGGGMAFTFLKALGYNVGKSLV
EEDLIETARSTYAKAREKGIKFYLPVDCVAADRFNPEAETKVTTIQEIPEEWMALDIGPATVALFTEALQSAKTIIWNGP
MGVFEMDAFSRGTFAMVTAVANSYALTIVGGGDTDSAVHRAGEYAKISYISTGGGAFLELLEGKKLPRHQGAGRKRKIGG
AAMRTPVIAGNWKLFKKRAEARELVNGLVSLVKDVQGVEIVVAPVFTVLAAVKGALDGSAIKLAGQDCFWEEEGAYTGEI
SPGMLVDAGCSHVIIGHSERRQYFGETDETVNRKIKAALKAGLAAIACIGESLAEREAGTTFDVLRTQLNGGLAGLSADD
LKDVIIAYEPVWAIGTGKTATDAQAQEAHAFIRGVVSELLGKPAAEAVRILYGGSVKPENIKGLMTQPDIDGALVGGASL
KADSFAAIASFSM

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=400, Percent_Identity=47, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI31543397, Length=400, Percent_Identity=43.75, Blast_Score=334, Evalue=2e-91,
Organism=Homo sapiens, GI226529917, Length=273, Percent_Identity=43.956043956044, Blast_Score=207, Evalue=2e-53,
Organism=Homo sapiens, GI4507645, Length=246, Percent_Identity=46.3414634146341, Blast_Score=203, Evalue=5e-52,
Organism=Escherichia coli, GI1789294, Length=380, Percent_Identity=44.7368421052632, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI1790353, Length=246, Percent_Identity=47.1544715447154, Blast_Score=206, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17508823, Length=404, Percent_Identity=46.7821782178218, Blast_Score=345, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI17536593, Length=244, Percent_Identity=47.1311475409836, Blast_Score=198, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6319857, Length=398, Percent_Identity=48.4924623115578, Blast_Score=353, Evalue=5e-98,
Organism=Saccharomyces cerevisiae, GI6320255, Length=247, Percent_Identity=41.7004048582996, Blast_Score=175, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24581203, Length=396, Percent_Identity=44.9494949494949, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI17136394, Length=399, Percent_Identity=44.8621553884712, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI28572004, Length=240, Percent_Identity=45.8333333333333, Blast_Score=195, Evalue=8e-50,
Organism=Drosophila melanogaster, GI28572008, Length=240, Percent_Identity=45.8333333333333, Blast_Score=194, Evalue=2e-49,
Organism=Drosophila melanogaster, GI28572006, Length=240, Percent_Identity=45.8333333333333, Blast_Score=194, Evalue=2e-49,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 70341; Mature: 70210

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00111 PGLYCERATE_KINASE ; PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRYIDEISELKDKKVFIRVDFNVPLDEHQNITEDTRIRAVLPTINFALDAGAKVVLAS
CCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECHHHHHHCCCCHHHHHH
HLGRPKGERKPKYSMAPAAKRLSRLLGKEVLLAPDCIGSEVRQMIDAMKSGDIIMLENVR
HCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCHHCCHHHHHHHHHHCCCCEEEEECEE
FYEGEEKNDENFARELANGCEIYVNDAFAVSHRAHASVEAITKFFPVVAAGFLMKNEMNY
EECCCCCCHHHHHHHHCCCCEEEEECCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEKAMQKPIRPLVAILGGAKVSGKLEVLESLLNKVDKIIIGGGMAFTFLKALGYNVGKSL
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHH
VEEDLIETARSTYAKAREKGIKFYLPVDCVAADRFNPEAETKVTTIQEIPEEWMALDIGP
HHHHHHHHHHHHHHHHHHCCCEEEECCEEEECCCCCCCCHHHHHHHHHCCHHHHEEECCH
ATVALFTEALQSAKTIIWNGPMGVFEMDAFSRGTFAMVTAVANSYALTIVGGGDTDSAVH
HHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCHHHHH
RAGEYAKISYISTGGGAFLELLEGKKLPRHQGAGRKRKIGGAAMRTPVIAGNWKLFKKRA
HCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCEEECCHHHHHHHH
EARELVNGLVSLVKDVQGVEIVVAPVFTVLAAVKGALDGSAIKLAGQDCFWEEEGAYTGE
HHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEECC
ISPGMLVDAGCSHVIIGHSERRQYFGETDETVNRKIKAALKAGLAAIACIGESLAEREAG
CCCCEEEECCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TTFDVLRTQLNGGLAGLSADDLKDVIIAYEPVWAIGTGKTATDAQAQEAHAFIRGVVSEL
CHHHHHHHHHCCCCCCCCHHHHHHHHEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHH
LGKPAAEAVRILYGGSVKPENIKGLMTQPDIDGALVGGASLKADSFAAIASFSM
HCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
AIRYIDEISELKDKKVFIRVDFNVPLDEHQNITEDTRIRAVLPTINFALDAGAKVVLAS
CCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEECHHHHHHCCCCHHHHHH
HLGRPKGERKPKYSMAPAAKRLSRLLGKEVLLAPDCIGSEVRQMIDAMKSGDIIMLENVR
HCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCHHCCHHHHHHHHHHCCCCEEEEECEE
FYEGEEKNDENFARELANGCEIYVNDAFAVSHRAHASVEAITKFFPVVAAGFLMKNEMNY
EECCCCCCHHHHHHHHCCCCEEEEECCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FEKAMQKPIRPLVAILGGAKVSGKLEVLESLLNKVDKIIIGGGMAFTFLKALGYNVGKSL
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHH
VEEDLIETARSTYAKAREKGIKFYLPVDCVAADRFNPEAETKVTTIQEIPEEWMALDIGP
HHHHHHHHHHHHHHHHHHCCCEEEECCEEEECCCCCCCCHHHHHHHHHCCHHHHEEECCH
ATVALFTEALQSAKTIIWNGPMGVFEMDAFSRGTFAMVTAVANSYALTIVGGGDTDSAVH
HHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCHHHHH
RAGEYAKISYISTGGGAFLELLEGKKLPRHQGAGRKRKIGGAAMRTPVIAGNWKLFKKRA
HCCCCEEEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCEEECCHHHHHHHH
EARELVNGLVSLVKDVQGVEIVVAPVFTVLAAVKGALDGSAIKLAGQDCFWEEEGAYTGE
HHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEECC
ISPGMLVDAGCSHVIIGHSERRQYFGETDETVNRKIKAALKAGLAAIACIGESLAEREAG
CCCCEEEECCCCEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TTFDVLRTQLNGGLAGLSADDLKDVIIAYEPVWAIGTGKTATDAQAQEAHAFIRGVVSEL
CHHHHHHHHHCCCCCCCCHHHHHHHHEEECCEEEECCCCCCCCHHHHHHHHHHHHHHHHH
LGKPAAEAVRILYGGSVKPENIKGLMTQPDIDGALVGGASLKADSFAAIASFSM
HCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA