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The map label for this gene is xth [H]

Identifier: 39996639

GI number: 39996639

Start: 1692893

End: 1693708

Strand: Direct

Name: xth [H]

Synonym: GSU1539

Alternate gene names: 39996639

Gene position: 1692893-1693708 (Clockwise)

Preceding gene: 39996638

Following gene: 39996640

Centisome position: 44.38

GC content: 67.65

Gene sequence:

>816_bases
ATGAAACTCGTATCCTTCAACGTCAATGGCCTGCGCTCCCGTCTCCATCAACTCGAAGAACTGGTCCGGACCCATCGGCC
CGACATCATCGGCCTCCAGGAAACCAAGGTGCAGGATGCCGACTTCCCCCTGGCCGCCGTGCAGGCCCTGGGCTATCACG
TGATCTACCACGGCCAGAAGACCCACCACGGCGTGGCGCTCCTCTCCCTCCAGCCGCCCCGCGACGTCCGGCTCGGCCTC
CCCGGCGACGGCGACGAGGCCCAGAAGCGCTTCATCGGCGCCACCTTCGACCTCCCCGCCGGGCCGCCCCTGAGGGTCAT
CAACGGCTACTTCCCCCAGGGCGAAAGCCGCGACCACCCGGTCAAGTTCCCGGCCAAGGAACGCTTCTACGCCGACGTGC
TCGCCTACCTGAAATCCTCCTGCGACCCCGACGCCCCCCTGGCGGTCATGGGAGACTTCAACATCGCCCCCGTGGACCCG
GACATCGGCATCGGCGCCGACAACGCCAAGCGCTGGCTCCGCACCGGCAAGACCAGCTTCCTCCCCGAAGAGCGGGCCTG
GTTCGCCGCCCTGCGCGACTGGGGGCTGCACGACAGCTACCGTGAACTCTACCCCGACATCGACGACCGCTTCAGCTGGT
TCGACTACCGCAGCCGCGGCTTCGAGTCCGAGCCCAAGCGGGGCCTGCGCATCGACCACATCCTCCTCACCCGTCCGCTC
CAGCAAGCCTGCCGCGCCGCCGGCATCGACTACGACATCCGCGCCATGGAAAAGCCGTCCGACCACTGCCCCGTGTGGGT
GGAGGTGGATGTGTGA

Upstream 100 bases:

>100_bases
ACATTTTCGCCCGCCTCGAGCCCGTTCACAAACCAATGGATAATGAACCCGCAATCTGCTAGTGATACGAGCACAATTCC
CCTTTGCGAGGTGACCCGGG

Downstream 100 bases:

>100_bases
TCATCGCCTGAAACACGGCCCGAGGTTGCGGCGTATAGGGGTTTTCCTGATTGAACGGAGATGGTAATTGATTTATGAAA
GGGTGTTAGTGATGCGTGGA

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKLVSFNVNGLRSRLHQLEELVRTHRPDIIGLQETKVQDADFPLAAVQALGYHVIYHGQKTHHGVALLSLQPPRDVRLGL
PGDGDEAQKRFIGATFDLPAGPPLRVINGYFPQGESRDHPVKFPAKERFYADVLAYLKSSCDPDAPLAVMGDFNIAPVDP
DIGIGADNAKRWLRTGKTSFLPEERAWFAALRDWGLHDSYRELYPDIDDRFSWFDYRSRGFESEPKRGLRIDHILLTRPL
QQACRAAGIDYDIRAMEKPSDHCPVWVEVDV

Sequences:

>Translated_271_residues
MKLVSFNVNGLRSRLHQLEELVRTHRPDIIGLQETKVQDADFPLAAVQALGYHVIYHGQKTHHGVALLSLQPPRDVRLGL
PGDGDEAQKRFIGATFDLPAGPPLRVINGYFPQGESRDHPVKFPAKERFYADVLAYLKSSCDPDAPLAVMGDFNIAPVDP
DIGIGADNAKRWLRTGKTSFLPEERAWFAALRDWGLHDSYRELYPDIDDRFSWFDYRSRGFESEPKRGLRIDHILLTRPL
QQACRAAGIDYDIRAMEKPSDHCPVWVEVDV
>Mature_271_residues
MKLVSFNVNGLRSRLHQLEELVRTHRPDIIGLQETKVQDADFPLAAVQALGYHVIYHGQKTHHGVALLSLQPPRDVRLGL
PGDGDEAQKRFIGATFDLPAGPPLRVINGYFPQGESRDHPVKFPAKERFYADVLAYLKSSCDPDAPLAVMGDFNIAPVDP
DIGIGADNAKRWLRTGKTSFLPEERAWFAALRDWGLHDSYRELYPDIDDRFSWFDYRSRGFESEPKRGLRIDHILLTRPL
QQACRAAGIDYDIRAMEKPSDHCPVWVEVDV

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=271, Percent_Identity=28.4132841328413, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI18375503, Length=271, Percent_Identity=28.4132841328413, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI18375501, Length=271, Percent_Identity=28.4132841328413, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=60.5263157894737, Blast_Score=332, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI71989536, Length=275, Percent_Identity=24, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI221330655, Length=275, Percent_Identity=27.6363636363636, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI17136678, Length=275, Percent_Identity=27.6363636363636, Blast_Score=77, Evalue=9e-15,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30710; Mature: 30710

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVSFNVNGLRSRLHQLEELVRTHRPDIIGLQETKVQDADFPLAAVQALGYHVIYHGQK
CEEEEECHHHHHHHHHHHHHHHHHCCCCEECCHHCCCCCCCCCHHHHHHHCEEEEECCCC
THHGVALLSLQPPRDVRLGLPGDGDEAQKRFIGATFDLPAGPPLRVINGYFPQGESRDHP
CCCCEEEEEECCCCCEEECCCCCCHHHHHHHCCCEECCCCCCCCEEEECCCCCCCCCCCC
VKFPAKERFYADVLAYLKSSCDPDAPLAVMGDFNIAPVDPDIGIGADNAKRWLRTGKTSF
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEECCCCCCCCCCHHHHHHHHCCCCCC
LPEERAWFAALRDWGLHDSYRELYPDIDDRFSWFDYRSRGFESEPKRGLRIDHILLTRPL
CCHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHH
QQACRAAGIDYDIRAMEKPSDHCPVWVEVDV
HHHHHHCCCCCEEEECCCCCCCCCEEEEECC
>Mature Secondary Structure
MKLVSFNVNGLRSRLHQLEELVRTHRPDIIGLQETKVQDADFPLAAVQALGYHVIYHGQK
CEEEEECHHHHHHHHHHHHHHHHHCCCCEECCHHCCCCCCCCCHHHHHHHCEEEEECCCC
THHGVALLSLQPPRDVRLGLPGDGDEAQKRFIGATFDLPAGPPLRVINGYFPQGESRDHP
CCCCEEEEEECCCCCEEECCCCCCHHHHHHHCCCEECCCCCCCCEEEECCCCCCCCCCCC
VKFPAKERFYADVLAYLKSSCDPDAPLAVMGDFNIAPVDPDIGIGADNAKRWLRTGKTSF
CCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEECCCCCCCCCCHHHHHHHHCCCCCC
LPEERAWFAALRDWGLHDSYRELYPDIDDRFSWFDYRSRGFESEPKRGLRIDHILLTRPL
CCHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHH
QQACRAAGIDYDIRAMEKPSDHCPVWVEVDV
HHHHHHCCCCCEEEECCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]