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The map label for this gene is thrS

Identifier: 39996615

GI number: 39996615

Start: 1662153

End: 1664063

Strand: Direct

Name: thrS

Synonym: GSU1515

Alternate gene names: 39996615

Gene position: 1662153-1664063 (Clockwise)

Preceding gene: 39996614

Following gene: 39996616

Centisome position: 43.58

GC content: 59.08

Gene sequence:

>1911_bases
ATGGGAGACATCAGGGTAACGCTGCCGGATGGTTCAGAAAGAATTCTTGCCGAGGGCGCCACGGTGCGCGATCTTGCCGC
CTCCATCGGGGCAGGGCTTGCCAAGGCTGCCATAGCCGGAAAGATCGACGGGGTTCAGGTCGATGTTACCGCCCCCCTGA
CCGACGGCGCCAGGGTCGAGATCATTACCGATAAGTCTGCGGATGGCCTTGACATCATCAGGCACTCCACCTCCCACCTC
ATGGCCCAGGCCGTCAAGCAGCTTTTCCCGACCGCCAAGGTTACCATCGGCCCTGCCGTTGAAACGGGCTTCTATTACGA
TTTCGACGTAGAAACCCCATTCAGTCCCGAAGATCTTGCCCGCATCGAGGAGCGGATGCGAGAGCTAGCCAAGGCCGACA
TTCCTGTCGAGCGCCAGGTGCTTTCCCGTGACGAGGCAGTGAAGCTTTTTCGTGACATGGGTGAAGAATACAAGGTCGAG
ATCATCGAGGCACTCCCCGAGGAGAGCGTTTCGCTCTATCGCCAGGGCGATTTCGTTGACCTGTGCCGCGGTCCTCACCT
CCCTTCGACTTCCTTTTGCCGGGCGTTCAAGCTGACCTCTCTGGCCGGCGCCTACTGGCGCGGGGACGAGAAGAACAAGA
TGCTCCAGCGGGTCTACGGAACCGCCTTTGCGGACAAGAAGGAGCTCGACGCCTATCTGGAGAAGATCGAGGAGGCCAAG
CGCCGCGACCATCGCAAGCTGGGCCGGGAGCTCGACCTCTTTTCCTTCTCTGACGATGTGGGGGCCGGTTTCGTCATCTG
GCATCCGAAGGGTGCCATGCTTCGTACCCTGCTGGAGGATTTCGAGCGTCGGGAGCATCTCAAACGCGGCTATGACATCG
TGGTCGGTCCCCAGATCCTGAAGACCGAACTCTGGCAGCGTTCCGGCCACTACGACAATTACCGCGAGAACATGTATTTC
ACCGAGGTTGAGGGGCAGGGGTTCGGCATCAAGCCGATGAACTGTCTTTCCCACATGATGATTTACAAGTCCCAGTTGCG
CAGCTACCGGGACCTGCCGCTCCGTTTCTTCGAGCTCGGCACGGTGCATCGCCATGAGCGCGCTGGCGTGCTGCACGGCC
TCCTGCGGGTCCGCTGCTTCACCCAGGACGATGCCCATATTCTCTGTACCCCCGAGCAGCTTGACGCCGAGATCAAGGGA
GTCATCTCCTTTGTGAACGACGTCATGGGCATATTCGGATTCGACTACGAGATGGAGCTTTCTACCCGGCCCGAGAAGTC
CATCGGCTCCGACGAGGACTGGGAGCGCGCCACCGGTGCCCTGCTGGGTGCCCTCAAGGATTCGGGCAGGCCCTACGAGA
TCAACGAGGGAGACGGCGCATTTTACGGGCCGAAGATCGACATCAAGCTGCGCGATTCCCTTGACAGAAAGTGGCAGTGT
GCTACAATCCAGTGCGATTTTACGCTCCCTGAACGGTTTGACCTGACCTATGTGGATGCGGATGGCGAGCGCAAGCGGCC
GGTCATGGTCCATCGGGTCATTCTCGGGTCCATCGAGCGGTTCATCGGTGTCCTTATTGAGCACTTCGCCGGCAATTTCC
CGCTGTGGCTCTCGCCGGTTCAGGCCATTGTGCTGACCGTTACCGACAACCAGCAGCCCTACGCGGCGCAGGTGTTCGAG
ACGCTTCGGACAAACGGGGTGAGAGTTCAGAAGGATTTTCGCAACGAAAAGCTCGGGTTCAAGATCCGCGAGGCGCAGCT
GCAGAAAATCCCCTACATGCTCGTCATCGGTGACAAGGAAGTCGAATCAGGTACGGTAACTCCCCGGTTCCGCGACGGGT
CAAACCTCGCGGCCATGAAGCCGGAGGAGTTTGTTGCGTTTGTAAACGACGAAGTGAAACGCTTCAATTAG

Upstream 100 bases:

>100_bases
GTTCGAGACCGCCCGCGCCTACCATTCAAAAAATGCTCGCGGCGAACGCTGTGGAAGGGGCATCGAAGGTTCGATGCCTC
TTTCTGTTAAGGGGCCAGTG

Downstream 100 bases:

>100_bases
GAGGTGGTGCCATAGCGAAGCCTACGGTAAATATCAATCAGGCCATACGGGCGCGCGAGGTGCGCGTTGTCGGCGCAGAC
AGCGAACAGCTGGGGATCAT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 636; Mature: 635

Protein sequence:

>636_residues
MGDIRVTLPDGSERILAEGATVRDLAASIGAGLAKAAIAGKIDGVQVDVTAPLTDGARVEIITDKSADGLDIIRHSTSHL
MAQAVKQLFPTAKVTIGPAVETGFYYDFDVETPFSPEDLARIEERMRELAKADIPVERQVLSRDEAVKLFRDMGEEYKVE
IIEALPEESVSLYRQGDFVDLCRGPHLPSTSFCRAFKLTSLAGAYWRGDEKNKMLQRVYGTAFADKKELDAYLEKIEEAK
RRDHRKLGRELDLFSFSDDVGAGFVIWHPKGAMLRTLLEDFERREHLKRGYDIVVGPQILKTELWQRSGHYDNYRENMYF
TEVEGQGFGIKPMNCLSHMMIYKSQLRSYRDLPLRFFELGTVHRHERAGVLHGLLRVRCFTQDDAHILCTPEQLDAEIKG
VISFVNDVMGIFGFDYEMELSTRPEKSIGSDEDWERATGALLGALKDSGRPYEINEGDGAFYGPKIDIKLRDSLDRKWQC
ATIQCDFTLPERFDLTYVDADGERKRPVMVHRVILGSIERFIGVLIEHFAGNFPLWLSPVQAIVLTVTDNQQPYAAQVFE
TLRTNGVRVQKDFRNEKLGFKIREAQLQKIPYMLVIGDKEVESGTVTPRFRDGSNLAAMKPEEFVAFVNDEVKRFN

Sequences:

>Translated_636_residues
MGDIRVTLPDGSERILAEGATVRDLAASIGAGLAKAAIAGKIDGVQVDVTAPLTDGARVEIITDKSADGLDIIRHSTSHL
MAQAVKQLFPTAKVTIGPAVETGFYYDFDVETPFSPEDLARIEERMRELAKADIPVERQVLSRDEAVKLFRDMGEEYKVE
IIEALPEESVSLYRQGDFVDLCRGPHLPSTSFCRAFKLTSLAGAYWRGDEKNKMLQRVYGTAFADKKELDAYLEKIEEAK
RRDHRKLGRELDLFSFSDDVGAGFVIWHPKGAMLRTLLEDFERREHLKRGYDIVVGPQILKTELWQRSGHYDNYRENMYF
TEVEGQGFGIKPMNCLSHMMIYKSQLRSYRDLPLRFFELGTVHRHERAGVLHGLLRVRCFTQDDAHILCTPEQLDAEIKG
VISFVNDVMGIFGFDYEMELSTRPEKSIGSDEDWERATGALLGALKDSGRPYEINEGDGAFYGPKIDIKLRDSLDRKWQC
ATIQCDFTLPERFDLTYVDADGERKRPVMVHRVILGSIERFIGVLIEHFAGNFPLWLSPVQAIVLTVTDNQQPYAAQVFE
TLRTNGVRVQKDFRNEKLGFKIREAQLQKIPYMLVIGDKEVESGTVTPRFRDGSNLAAMKPEEFVAFVNDEVKRFN
>Mature_635_residues
GDIRVTLPDGSERILAEGATVRDLAASIGAGLAKAAIAGKIDGVQVDVTAPLTDGARVEIITDKSADGLDIIRHSTSHLM
AQAVKQLFPTAKVTIGPAVETGFYYDFDVETPFSPEDLARIEERMRELAKADIPVERQVLSRDEAVKLFRDMGEEYKVEI
IEALPEESVSLYRQGDFVDLCRGPHLPSTSFCRAFKLTSLAGAYWRGDEKNKMLQRVYGTAFADKKELDAYLEKIEEAKR
RDHRKLGRELDLFSFSDDVGAGFVIWHPKGAMLRTLLEDFERREHLKRGYDIVVGPQILKTELWQRSGHYDNYRENMYFT
EVEGQGFGIKPMNCLSHMMIYKSQLRSYRDLPLRFFELGTVHRHERAGVLHGLLRVRCFTQDDAHILCTPEQLDAEIKGV
ISFVNDVMGIFGFDYEMELSTRPEKSIGSDEDWERATGALLGALKDSGRPYEINEGDGAFYGPKIDIKLRDSLDRKWQCA
TIQCDFTLPERFDLTYVDADGERKRPVMVHRVILGSIERFIGVLIEHFAGNFPLWLSPVQAIVLTVTDNQQPYAAQVFET
LRTNGVRVQKDFRNEKLGFKIREAQLQKIPYMLVIGDKEVESGTVTPRFRDGSNLAAMKPEEFVAFVNDEVKRFN

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=616, Percent_Identity=40.7467532467533, Blast_Score=476, Evalue=1e-134,
Organism=Homo sapiens, GI38570111, Length=611, Percent_Identity=39.607201309329, Blast_Score=474, Evalue=1e-133,
Organism=Homo sapiens, GI20070344, Length=636, Percent_Identity=38.0503144654088, Blast_Score=421, Evalue=1e-118,
Organism=Escherichia coli, GI1788013, Length=631, Percent_Identity=49.6038034865293, Blast_Score=657, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984192, Length=619, Percent_Identity=39.5799676898223, Blast_Score=489, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI71984184, Length=619, Percent_Identity=39.5799676898223, Blast_Score=489, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6322112, Length=630, Percent_Identity=40.3174603174603, Blast_Score=482, Evalue=1e-137,
Organism=Saccharomyces cerevisiae, GI6322654, Length=393, Percent_Identity=39.9491094147583, Blast_Score=290, Evalue=4e-79,
Organism=Drosophila melanogaster, GI24583842, Length=614, Percent_Identity=40.7166123778502, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI28574141, Length=614, Percent_Identity=40.7166123778502, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI24583839, Length=614, Percent_Identity=40.7166123778502, Blast_Score=488, Evalue=1e-138,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_GEOSL (Q74D04)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952566.1
- ProteinModelPortal:   Q74D04
- SMR:   Q74D04
- GeneID:   2687721
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1515
- NMPDR:   fig|243231.1.peg.1504
- TIGR:   GSU1515
- HOGENOM:   HBG352811
- OMA:   TAKDPEC
- ProtClustDB:   PRK00413
- BioCyc:   GSUL243231:GSU_1515-MONOMER
- BRENDA:   6.1.1.3
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 72146; Mature: 72015

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDIRVTLPDGSERILAEGATVRDLAASIGAGLAKAAIAGKIDGVQVDVTAPLTDGARVE
CCCEEEECCCCCCCHHCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEECCCCCCEEE
IITDKSADGLDIIRHSTSHLMAQAVKQLFPTAKVTIGPAVETGFYYDFDVETPFSPEDLA
EEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCCHHHHH
RIEERMRELAKADIPVERQVLSRDEAVKLFRDMGEEYKVEIIEALPEESVSLYRQGDFVD
HHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCCCHH
LCRGPHLPSTSFCRAFKLTSLAGAYWRGDEKNKMLQRVYGTAFADKKELDAYLEKIEEAK
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RRDHRKLGRELDLFSFSDDVGAGFVIWHPKGAMLRTLLEDFERREHLKRGYDIVVGPQIL
HHHHHHHCCCCCEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHH
KTELWQRSGHYDNYRENMYFTEVEGQGFGIKPMNCLSHMMIYKSQLRSYRDLPLRFFELG
HHHHHHHCCCCCCHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHC
TVHRHERAGVLHGLLRVRCFTQDDAHILCTPEQLDAEIKGVISFVNDVMGIFGFDYEMEL
CHHHHHHHHHHHHHHHHEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
STRPEKSIGSDEDWERATGALLGALKDSGRPYEINEGDGAFYGPKIDIKLRDSLDRKWQC
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCEECCEEEEEECCCCCCCEEE
ATIQCDFTLPERFDLTYVDADGERKRPVMVHRVILGSIERFIGVLIEHFAGNFPLWLSPV
EEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
QAIVLTVTDNQQPYAAQVFETLRTNGVRVQKDFRNEKLGFKIREAQLQKIPYMLVIGDKE
EEEEEEEECCCCCHHHHHHHHHHCCCEEEEHHHCCCCCCCEEHHHHHHHCCEEEEECCCC
VESGTVTPRFRDGSNLAAMKPEEFVAFVNDEVKRFN
CCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GDIRVTLPDGSERILAEGATVRDLAASIGAGLAKAAIAGKIDGVQVDVTAPLTDGARVE
CCEEEECCCCCCCHHCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEEEEECCCCCCEEE
IITDKSADGLDIIRHSTSHLMAQAVKQLFPTAKVTIGPAVETGFYYDFDVETPFSPEDLA
EEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCCHHHHH
RIEERMRELAKADIPVERQVLSRDEAVKLFRDMGEEYKVEIIEALPEESVSLYRQGDFVD
HHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHCCCCHH
LCRGPHLPSTSFCRAFKLTSLAGAYWRGDEKNKMLQRVYGTAFADKKELDAYLEKIEEAK
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
RRDHRKLGRELDLFSFSDDVGAGFVIWHPKGAMLRTLLEDFERREHLKRGYDIVVGPQIL
HHHHHHHCCCCCEEECCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHH
KTELWQRSGHYDNYRENMYFTEVEGQGFGIKPMNCLSHMMIYKSQLRSYRDLPLRFFELG
HHHHHHHCCCCCCHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEHHC
TVHRHERAGVLHGLLRVRCFTQDDAHILCTPEQLDAEIKGVISFVNDVMGIFGFDYEMEL
CHHHHHHHHHHHHHHHHEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
STRPEKSIGSDEDWERATGALLGALKDSGRPYEINEGDGAFYGPKIDIKLRDSLDRKWQC
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCEECCEEEEEECCCCCCCEEE
ATIQCDFTLPERFDLTYVDADGERKRPVMVHRVILGSIERFIGVLIEHFAGNFPLWLSPV
EEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
QAIVLTVTDNQQPYAAQVFETLRTNGVRVQKDFRNEKLGFKIREAQLQKIPYMLVIGDKE
EEEEEEEECCCCCHHHHHHHHHHCCCEEEEHHHCCCCCCCEEHHHHHHHCCEEEEECCCC
VESGTVTPRFRDGSNLAAMKPEEFVAFVNDEVKRFN
CCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA