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The map label for this gene is icaA [H]

Identifier: 39996554

GI number: 39996554

Start: 1592452

End: 1593870

Strand: Direct

Name: icaA [H]

Synonym: GSU1454

Alternate gene names: 39996554

Gene position: 1592452-1593870 (Clockwise)

Preceding gene: 39996553

Following gene: 39996555

Centisome position: 41.75

GC content: 56.45

Gene sequence:

>1419_bases
ATGCTTCATAACATCGTTGCCTTCCTGCTGGCGATCCAGCCGGTGATCTTCATTTATTTCATCGTGCTGAACGGGTTCTA
CACCCTGTTCACCATCATCTCCCTGCGTGACATCCGCAACTATCTGAATGCCGTCACGAGCCAGAGCATCGACAACGTGC
TCAACGGGATGTTCTATCGGCCCCTGTCTATCCTCGTGCCCGCCTACAACGAGGAAAAGACCATCGTTTCCTCGGTGAAG
TCGCTGCTCGCCCTGCGGTATCCCGAGTACGAAGTCATCGTCATCAACGACGGGTCCACCGACGGCACTCTTGAAAGTCT
CATCAACGAATTCCGTCTTGTCCGGATCGACAAGCCCATCAGCCTGCATGTTCCCCATCGGCCGATCATTGCCAAGTACG
TTTCCGTCGACCATCCCTACCTCTTCGTGCTGGACAAGGAGAACGGCGGCAAGGCCGATGCCCTCAACGCGGGGATAAAC
GCTTCCCAGTTTCCCCTGTTCTGCTCGATCGATGCCGACTCCGTGCTTGAGGACGATGCGCTCATCCGTGCGACGCGGCT
TTTCGTGGAAGACCGGGAGGTCGTTGCCACCGGCGGTATCGTGCGGGTCCTTAACGGTTGCGAGGTGGAGGACGGCATCG
TCAAGGGGGTCCGCGCTCCCCGGGGGATGCTGGAGTGCTTCCAGACTGTGGAGTACACCAAGGGGTTCCTCTCGGGGCGG
ACATCGTGGAATTACTTCAGGAGCTTGCTGATCATCTCGGGCGCCTTCGGCATCTTCCGCAAGGACATGGTGATGGCCGT
CAAAGGGTACCGCGAGAGCGTGGGCGAGGACATGGACTTGGTGGTGCGGCTCCACCGCCATTGCCGCCAGAACCGGATTC
GCTACAAGGTAGTGTTTGTGCCCGATCCTGTCTGTTGGACCCAGGTTCCTTCGGATATGGCATCGCTCCTCAAACAGCGC
AATCGCTGGCACCGTGGCCTCATTGACAGCCTTTGGCACAACAGGGGCATGTTCCTCAACCCCCGCTACGGTACGGTCGG
ACTGTTCGGGTTCCCCTATTTTGTTACGGTTGAACTACTCGGGCCGGCAGTTGAATTTATTGGCTATTTCGGCTTCGTGC
TTCTCTTTTTCCTGGGTCAGGTGAATCGCGAGTTTGCAATTCTCTTTTTCCTCCTGGCTGTTCTCTGGGGAACTTGGATT
AATCTCGGCTCCATCTTTCTCGATAACCTCATTTACAAGCGCTACAAGGGGTTGGGCGACGTCCTGAAGCTTTGCCTCTT
CGGTCTACTCGAATTTTTCGGGTACCGGCAGATCATCGTGGTTGAACGGCTCATCGCCACGTTCATGTTCTGGAAAAAGG
GGTGGGGCAAGCCCAAGCGAAAGGAGATCGATGGTGAAGTGTCTGGTTCGGTTGCCTAG

Upstream 100 bases:

>100_bases
AGAATGGGAGAGGCGGGGCTCGAAGTCCTCGTGGATGCAACCCGGAGCGAGGACCGGTTCGTGCGCGATGTTTCTCAATA
CGTGCTGAAGAGGCTCATCT

Downstream 100 bases:

>100_bases
CAGGATACTCTATCTGGTGCCGCGGGTGCTGCTCCTGGCCCTGTTCCTGACCTACGTGACGTTCATGCTCCTGTCTTCCT
ATGAATGGTTCCGTCCCGTG

Product: glycosyl transferase, group 2 family protein

Products: NA

Alternate protein names: PNAG synthase; Poly-beta-1,6-GlcNAc synthase; Biofilm polysaccharide intercellular adhesin synthesis protein IcaA; Biofilm PIA synthesis protein IcaA; Intercellular adhesion protein A; N-acetylglucosaminyltransferase IcaA [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MLHNIVAFLLAIQPVIFIYFIVLNGFYTLFTIISLRDIRNYLNAVTSQSIDNVLNGMFYRPLSILVPAYNEEKTIVSSVK
SLLALRYPEYEVIVINDGSTDGTLESLINEFRLVRIDKPISLHVPHRPIIAKYVSVDHPYLFVLDKENGGKADALNAGIN
ASQFPLFCSIDADSVLEDDALIRATRLFVEDREVVATGGIVRVLNGCEVEDGIVKGVRAPRGMLECFQTVEYTKGFLSGR
TSWNYFRSLLIISGAFGIFRKDMVMAVKGYRESVGEDMDLVVRLHRHCRQNRIRYKVVFVPDPVCWTQVPSDMASLLKQR
NRWHRGLIDSLWHNRGMFLNPRYGTVGLFGFPYFVTVELLGPAVEFIGYFGFVLLFFLGQVNREFAILFFLLAVLWGTWI
NLGSIFLDNLIYKRYKGLGDVLKLCLFGLLEFFGYRQIIVVERLIATFMFWKKGWGKPKRKEIDGEVSGSVA

Sequences:

>Translated_472_residues
MLHNIVAFLLAIQPVIFIYFIVLNGFYTLFTIISLRDIRNYLNAVTSQSIDNVLNGMFYRPLSILVPAYNEEKTIVSSVK
SLLALRYPEYEVIVINDGSTDGTLESLINEFRLVRIDKPISLHVPHRPIIAKYVSVDHPYLFVLDKENGGKADALNAGIN
ASQFPLFCSIDADSVLEDDALIRATRLFVEDREVVATGGIVRVLNGCEVEDGIVKGVRAPRGMLECFQTVEYTKGFLSGR
TSWNYFRSLLIISGAFGIFRKDMVMAVKGYRESVGEDMDLVVRLHRHCRQNRIRYKVVFVPDPVCWTQVPSDMASLLKQR
NRWHRGLIDSLWHNRGMFLNPRYGTVGLFGFPYFVTVELLGPAVEFIGYFGFVLLFFLGQVNREFAILFFLLAVLWGTWI
NLGSIFLDNLIYKRYKGLGDVLKLCLFGLLEFFGYRQIIVVERLIATFMFWKKGWGKPKRKEIDGEVSGSVA
>Mature_472_residues
MLHNIVAFLLAIQPVIFIYFIVLNGFYTLFTIISLRDIRNYLNAVTSQSIDNVLNGMFYRPLSILVPAYNEEKTIVSSVK
SLLALRYPEYEVIVINDGSTDGTLESLINEFRLVRIDKPISLHVPHRPIIAKYVSVDHPYLFVLDKENGGKADALNAGIN
ASQFPLFCSIDADSVLEDDALIRATRLFVEDREVVATGGIVRVLNGCEVEDGIVKGVRAPRGMLECFQTVEYTKGFLSGR
TSWNYFRSLLIISGAFGIFRKDMVMAVKGYRESVGEDMDLVVRLHRHCRQNRIRYKVVFVPDPVCWTQVPSDMASLLKQR
NRWHRGLIDSLWHNRGMFLNPRYGTVGLFGFPYFVTVELLGPAVEFIGYFGFVLLFFLGQVNREFAILFFLLAVLWGTWI
NLGSIFLDNLIYKRYKGLGDVLKLCLFGLLEFFGYRQIIVVERLIATFMFWKKGWGKPKRKEIDGEVSGSVA

Specific function: N-acetylglucosaminyltransferase that catalyzes the polymerization of single monomer units of UDP-N-acetylglucosamine to produce the linear homopolymer poly-beta-1,6-N-acetyl-D- glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide.

COG id: COG1215

COG function: function code M; Glycosyltransferases, probably involved in cell wall biogenesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 2 family [H]

Homologues:

Organism=Homo sapiens, GI20302153, Length=309, Percent_Identity=24.5954692556634, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1787259, Length=285, Percent_Identity=25.6140350877193, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 53816; Mature: 53816

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHNIVAFLLAIQPVIFIYFIVLNGFYTLFTIISLRDIRNYLNAVTSQSIDNVLNGMFYR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PLSILVPAYNEEKTIVSSVKSLLALRYPEYEVIVINDGSTDGTLESLINEFRLVRIDKPI
CHHHEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHEEEEECCCE
SLHVPHRPIIAKYVSVDHPYLFVLDKENGGKADALNAGINASQFPLFCSIDADSVLEDDA
EEECCCCHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCCCCCCCEEEECCCHHHHCCHH
LIRATRLFVEDREVVATGGIVRVLNGCEVEDGIVKGVRAPRGMLECFQTVEYTKGFLSGR
HHHHHHHHHHCCHHHHCCCHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
TSWNYFRSLLIISGAFGIFRKDMVMAVKGYRESVGEDMDLVVRLHRHCRQNRIRYKVVFV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEEEE
PDPVCWTQVPSDMASLLKQRNRWHRGLIDSLWHNRGMFLNPRYGTVGLFGFPYFVTVELL
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEECCHHHHHHHHH
GPAVEFIGYFGFVLLFFLGQVNREFAILFFLLAVLWGTWINLGSIFLDNLIYKRYKGLGD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
VLKLCLFGLLEFFGYRQIIVVERLIATFMFWKKGWGKPKRKEIDGEVSGSVA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLHNIVAFLLAIQPVIFIYFIVLNGFYTLFTIISLRDIRNYLNAVTSQSIDNVLNGMFYR
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PLSILVPAYNEEKTIVSSVKSLLALRYPEYEVIVINDGSTDGTLESLINEFRLVRIDKPI
CHHHEECCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHEEEEECCCE
SLHVPHRPIIAKYVSVDHPYLFVLDKENGGKADALNAGINASQFPLFCSIDADSVLEDDA
EEECCCCHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCCCCCCCEEEECCCHHHHCCHH
LIRATRLFVEDREVVATGGIVRVLNGCEVEDGIVKGVRAPRGMLECFQTVEYTKGFLSGR
HHHHHHHHHHCCHHHHCCCHHHHHCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCC
TSWNYFRSLLIISGAFGIFRKDMVMAVKGYRESVGEDMDLVVRLHRHCRQNRIRYKVVFV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEEEE
PDPVCWTQVPSDMASLLKQRNRWHRGLIDSLWHNRGMFLNPRYGTVGLFGFPYFVTVELL
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEECCHHHHHHHHH
GPAVEFIGYFGFVLLFFLGQVNREFAILFFLLAVLWGTWINLGSIFLDNLIYKRYKGLGD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
VLKLCLFGLLEFFGYRQIIVVERLIATFMFWKKGWGKPKRKEIDGEVSGSVA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10496925 [H]