Click here to switch to the map view.

The map label for this gene is hcaR [H]

Identifier: 39996444

GI number: 39996444

Start: 1468634

End: 1469542

Strand: Reverse

Name: hcaR [H]

Synonym: GSU1342

Alternate gene names: 39996444

Gene position: 1469542-1468634 (Counterclockwise)

Preceding gene: 39996472

Following gene: 39996438

Centisome position: 38.53

GC content: 63.37

Gene sequence:

>909_bases
ATGGAACTCAGACACTTGCGATACTTTGCCGCTACGGCGGAAGAGCTCAATTTCACCCGGGCCGCCAACAGGCTCCACAT
ATCGCAGCCCGCGGTCAGCCGCCTGATCAAAGAGCTGGAATGCGAGCTGGGAGCCCAGCTCTTTACCCGGGAACGGTTTG
GTTTGCGTCTGACTGCGGCAGGAGAGAAGTTTCTTTTTTACGCGCGCCGCGTTCTGCAGGATTGCGAGGAGGGAATACGT
GCGGTCAGGGAAAATGGGCTGAATCACCAGAAGCTCACCATCGGCTTCATCTCGACCGCCATGGAGTCGTTTCTCAGCGA
CGCCCTCGTGAGGCTGTCCCAGGAGCGGCCCGACATCGACGTCACGGTCAACGAGATGTCGCCCGGCGACCAGATTGCCG
CCCTGCGCTACCGGCAAATCGATCTGGGGTTCATCGGCAACCCCCACGACGGGGCCGGGGACGAATTCGAACTGTATGTC
TTGAGGGAAGTTCCCCTGGTGGCGGTGCTGCCGGTGCGCCACCGGCTGGCGGGACGTTCGGAGATATGCATCGGGGAGCT
TGAAGGCGACACCTTCATCGGCTACCTGGAGGACAAGTTCCCCGGCCGGAACCGGGTCATCAGCACTGCCTGCGCCAGGG
CGGGCTTCAGGCCCAAGTTCGAGTTGAAGGCCGGGTCGCTGATCGAGGTCCTGGGGATGATCGGGACGGGCAAGGGGGTA
TGCCTCATGCCGAGCGACGTGGCCGGCCTGCCCCATCCCAATGTAGCTTTCATCCCGCTCCGCGACCCCCTGGACCCGGT
CCGCCTGGCAGCGGCATGGCTTCCCGGCAACACCAACCCCGCCCTGCACCAGCTGATCGCTATCCTCGCAGCCGACTGCT
CCTCGGACACCGGTCGGACTTCACGCTGA

Upstream 100 bases:

>100_bases
GCCGAACAACACCTCTGATATCAGGATATTCCTCAATCTAATGAAGACAATAAAGTATTTATTTGACGTGCCATAACTTG
AGGTTATAGTTAAATTCATT

Downstream 100 bases:

>100_bases
ACGGAACCGTTGCGGGAAACGAGAGCCGCATCTTGAAGGTGGGCACAGACGTTACTAATTCGATGGAAAAGTTATTCCGG
AGATGCCGTAAGGTGTGAAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MELRHLRYFAATAEELNFTRAANRLHISQPAVSRLIKELECELGAQLFTRERFGLRLTAAGEKFLFYARRVLQDCEEGIR
AVRENGLNHQKLTIGFISTAMESFLSDALVRLSQERPDIDVTVNEMSPGDQIAALRYRQIDLGFIGNPHDGAGDEFELYV
LREVPLVAVLPVRHRLAGRSEICIGELEGDTFIGYLEDKFPGRNRVISTACARAGFRPKFELKAGSLIEVLGMIGTGKGV
CLMPSDVAGLPHPNVAFIPLRDPLDPVRLAAAWLPGNTNPALHQLIAILAADCSSDTGRTSR

Sequences:

>Translated_302_residues
MELRHLRYFAATAEELNFTRAANRLHISQPAVSRLIKELECELGAQLFTRERFGLRLTAAGEKFLFYARRVLQDCEEGIR
AVRENGLNHQKLTIGFISTAMESFLSDALVRLSQERPDIDVTVNEMSPGDQIAALRYRQIDLGFIGNPHDGAGDEFELYV
LREVPLVAVLPVRHRLAGRSEICIGELEGDTFIGYLEDKFPGRNRVISTACARAGFRPKFELKAGSLIEVLGMIGTGKGV
CLMPSDVAGLPHPNVAFIPLRDPLDPVRLAAAWLPGNTNPALHQLIAILAADCSSDTGRTSR
>Mature_302_residues
MELRHLRYFAATAEELNFTRAANRLHISQPAVSRLIKELECELGAQLFTRERFGLRLTAAGEKFLFYARRVLQDCEEGIR
AVRENGLNHQKLTIGFISTAMESFLSDALVRLSQERPDIDVTVNEMSPGDQIAALRYRQIDLGFIGNPHDGAGDEFELYV
LREVPLVAVLPVRHRLAGRSEICIGELEGDTFIGYLEDKFPGRNRVISTACARAGFRPKFELKAGSLIEVLGMIGTGKGV
CLMPSDVAGLPHPNVAFIPLRDPLDPVRLAAAWLPGNTNPALHQLIAILAADCSSDTGRTSR

Specific function: Transcriptional activator of the hca operon for 3- phenylpropionic acid catabolism [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788887, Length=299, Percent_Identity=33.1103678929766, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1787879, Length=262, Percent_Identity=32.824427480916, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI2367136, Length=297, Percent_Identity=31.3131313131313, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI157672245, Length=262, Percent_Identity=32.4427480916031, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87081904, Length=170, Percent_Identity=35.8823529411765, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI145693105, Length=265, Percent_Identity=26.7924528301887, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790399, Length=246, Percent_Identity=27.6422764227642, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1788297, Length=148, Percent_Identity=35.1351351351351, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790208, Length=248, Percent_Identity=25.8064516129032, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI48994962, Length=116, Percent_Identity=37.0689655172414, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788748, Length=254, Percent_Identity=25.1968503937008, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787601, Length=154, Percent_Identity=29.2207792207792, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787530, Length=277, Percent_Identity=27.0758122743682, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789204, Length=253, Percent_Identity=26.4822134387352, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33335; Mature: 33335

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRYFAATAEELNFTRAANRLHISQPAVSRLIKELECELGAQLFTRERFGLRLTAA
CCCHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
GEKFLFYARRVLQDCEEGIRAVRENGLNHQKLTIGFISTAMESFLSDALVRLSQERPDID
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCE
VTVNEMSPGDQIAALRYRQIDLGFIGNPHDGAGDEFELYVLREVPLVAVLPVRHRLAGRS
EEEECCCCCCCEEHEEEEEEEEEECCCCCCCCCCCEEEEEEECCCEEEEECHHHHHCCCC
EICIGELEGDTFIGYLEDKFPGRNRVISTACARAGFRPKFELKAGSLIEVLGMIGTGKGV
CEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEECCCHHHHHHHHHCCCCCE
CLMPSDVAGLPHPNVAFIPLRDPLDPVRLAAAWLPGNTNPALHQLIAILAADCSSDTGRT
EECCCCCCCCCCCCEEEEECCCCCCHHHHHEEECCCCCCHHHHHHHHHHHHCCCCCCCCC
SR
CC
>Mature Secondary Structure
MELRHLRYFAATAEELNFTRAANRLHISQPAVSRLIKELECELGAQLFTRERFGLRLTAA
CCCHHHHHHHHHHHHHHHHHHHCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
GEKFLFYARRVLQDCEEGIRAVRENGLNHQKLTIGFISTAMESFLSDALVRLSQERPDID
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCE
VTVNEMSPGDQIAALRYRQIDLGFIGNPHDGAGDEFELYVLREVPLVAVLPVRHRLAGRS
EEEECCCCCCCEEHEEEEEEEEEECCCCCCCCCCCEEEEEEECCCEEEEECHHHHHCCCC
EICIGELEGDTFIGYLEDKFPGRNRVISTACARAGFRPKFELKAGSLIEVLGMIGTGKGV
CEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEECCCHHHHHHHHHCCCCCE
CLMPSDVAGLPHPNVAFIPLRDPLDPVRLAAAWLPGNTNPALHQLIAILAADCSSDTGRT
EECCCCCCCCCCCCEEEEECCCCCCHHHHHEEECCCCCCHHHHHHHHHHHHCCCCCCCCC
SR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 9603882 [H]