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The map label for this gene is lepA

Identifier: 39996368

GI number: 39996368

Start: 1377368

End: 1379170

Strand: Direct

Name: lepA

Synonym: GSU1266

Alternate gene names: 39996368

Gene position: 1377368-1379170 (Clockwise)

Preceding gene: 39996364

Following gene: 39996369

Centisome position: 36.11

GC content: 60.23

Gene sequence:

>1803_bases
ATGAAGATTGATACCATCAGGAATTTTTCGATTATCGCCCACATTGACCACGGCAAATCCACCCTTGCGGATCGGCTGCT
GGAGTATACCGGCGCCCTCACGGAACGGGAGATGCAGGACCAGTTCCTGGACAAGATGGACCTGGAACGGGAGAGGGGTA
TCACCATCAAGGCCCAGACGGTACGGCTTACCTACCGGGCTGACGACGGTAACGACTACATCCTGAACCTCATCGACACG
CCGGGCCACGTGGACTTCACCTACGAGGTTTCCCGGTCCCTGGCCGCCTGCGAAGGTGCCCTGCTGGTGGTGGACGCCTC
CCAGGGGGTTGAAGCCCAGACCCTTGCCAACGTCTATCTCGCCATCGACAACAATCTGGAAGTCTTTCCGGTCCTCAACA
AGATCGACCTGCCCGCGGCCGAGCCGGAGCGGGTGAAGCACGAGATCGAGGAAATCATCGGTCTCGATGCCCACGACGCG
GTTATGGCCAGTGCCAAGGAGGGGATCGGCACTCGGGAGATCCTGGAGGAGATCGTCAAGAAGATTCCTCCTCCGGAAGG
AGATCCGGCCGCTCCGCTCAAGGCCCTGCTTTTCGATTCGTGGTACGACCAGTACCAAGGCGTGATCATCCTCGTCCGGG
TCATCGACGGCACCGTGAAGAAGGGGGACAAGATCCAGCTGGTCTCCACCGGCCGTAGTTACGAAGCCCTGAAGGTCGGT
GTCTTCGCCCCGGTGATGCGCGAAGTGCCCCAGCTGGCGGCGGGGGAGGTCGGTTTCCTCATTGCCGGCATCAAGGATGT
GGCTGACGCCAAGATCGGCGATACCGTCACCCACGCCCTCAAGCCCTGTGTAACGCCTCTCGGCGGTTTCAAGGAAGTGA
AGCCCATGGTCTTCTCGGGGCTCTATCCCATCGACACCGCCCAGTACGAGCAACTGCGCGATGCCCTGGCCAAGCTCAAG
CTGAACGACTCCTCCTTCTCGTTCGAGCCGGAGACATCGCTGGCCCTCGGTTTCGGTTTCCGCTGCGGCTTCCTGGGTCT
GCTCCACATGGAGATCATCCAGGAGCGCCTGGAGCGGGAGTTCAATCTCGACCTGATCACCACCGCCCCCACGGTTGTGT
ACAAGGTCCACCGGATCAAAGGGGACGTGATCACCATCGAGAGCGCCAACCAGTTGCCGCCGCTCCAGGAAATCGACTAC
ATCGAGGAGCCCTTCATCCTCGCCTCCATCCACACGCCCAACGAGTTCGTGGGGGGCATCCTCTCCCTGTGCGAGGAGAA
GCGCGGCGTCCAGCGCGAGATCAAGTACCTCACCCCCACGCGGGTGATGATCATCTATGAACTCCCCCTCAACGAGGTGG
TCCTCGACTTTTACGACCGGTTGAAATCAATCACCAAGGGGTACGCCTCCCTGGATTACGAGCATCTGAACTACCGCCGC
AGCGACCTGGTGCGGATGAACATCCTCATCAACGGCGAGGCCGTTGACGCGCTCTCCCTCATCATCCACCGGGACAAGGC
CTACTACCGGGGCCGTGACCTGGTCTCCAAGATGAAGGAGCTGATCCCGCGTCAGATGTTCGAGGTGGTCATTCAGGCGG
CCATCGGCGCCAAGGTCATCGCCCGCGAGACGGTGAAGGCCCTGCGCAAGGATGTACTCGCCAAGTGCTACGGCGGCGAC
ATCACCCGCAAGCGCAAGCTTTTGGAAAAGCAGAAGGAAGGAAAGAAGCGGATGAAAAACGTGGGGAACGTCGAACTCCC
CCAGGAAGCATTTCTCGCCATCCTCAAGGTGGAAGAGAAGTAG

Upstream 100 bases:

>100_bases
ATGCAGATGTGCCGTATACCGGCCGTGGAGAAAATGCCTTTACAACAGAGCTGGTTATCCATTATCCTTCATCGGTTACA
CTCAGAAGAGACAGGTGTTT

Downstream 100 bases:

>100_bases
TTACCCTTTCCGAAAGGAACATCAATGGACTACAAGGAAACCCAGTACGGCCAGTCAACCCCGTCGGAGCAGGCTGCCGA
GCCGGTCAAGAAAAAGCACA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MKIDTIRNFSIIAHIDHGKSTLADRLLEYTGALTEREMQDQFLDKMDLERERGITIKAQTVRLTYRADDGNDYILNLIDT
PGHVDFTYEVSRSLAACEGALLVVDASQGVEAQTLANVYLAIDNNLEVFPVLNKIDLPAAEPERVKHEIEEIIGLDAHDA
VMASAKEGIGTREILEEIVKKIPPPEGDPAAPLKALLFDSWYDQYQGVIILVRVIDGTVKKGDKIQLVSTGRSYEALKVG
VFAPVMREVPQLAAGEVGFLIAGIKDVADAKIGDTVTHALKPCVTPLGGFKEVKPMVFSGLYPIDTAQYEQLRDALAKLK
LNDSSFSFEPETSLALGFGFRCGFLGLLHMEIIQERLEREFNLDLITTAPTVVYKVHRIKGDVITIESANQLPPLQEIDY
IEEPFILASIHTPNEFVGGILSLCEEKRGVQREIKYLTPTRVMIIYELPLNEVVLDFYDRLKSITKGYASLDYEHLNYRR
SDLVRMNILINGEAVDALSLIIHRDKAYYRGRDLVSKMKELIPRQMFEVVIQAAIGAKVIARETVKALRKDVLAKCYGGD
ITRKRKLLEKQKEGKKRMKNVGNVELPQEAFLAILKVEEK

Sequences:

>Translated_600_residues
MKIDTIRNFSIIAHIDHGKSTLADRLLEYTGALTEREMQDQFLDKMDLERERGITIKAQTVRLTYRADDGNDYILNLIDT
PGHVDFTYEVSRSLAACEGALLVVDASQGVEAQTLANVYLAIDNNLEVFPVLNKIDLPAAEPERVKHEIEEIIGLDAHDA
VMASAKEGIGTREILEEIVKKIPPPEGDPAAPLKALLFDSWYDQYQGVIILVRVIDGTVKKGDKIQLVSTGRSYEALKVG
VFAPVMREVPQLAAGEVGFLIAGIKDVADAKIGDTVTHALKPCVTPLGGFKEVKPMVFSGLYPIDTAQYEQLRDALAKLK
LNDSSFSFEPETSLALGFGFRCGFLGLLHMEIIQERLEREFNLDLITTAPTVVYKVHRIKGDVITIESANQLPPLQEIDY
IEEPFILASIHTPNEFVGGILSLCEEKRGVQREIKYLTPTRVMIIYELPLNEVVLDFYDRLKSITKGYASLDYEHLNYRR
SDLVRMNILINGEAVDALSLIIHRDKAYYRGRDLVSKMKELIPRQMFEVVIQAAIGAKVIARETVKALRKDVLAKCYGGD
ITRKRKLLEKQKEGKKRMKNVGNVELPQEAFLAILKVEEK
>Mature_600_residues
MKIDTIRNFSIIAHIDHGKSTLADRLLEYTGALTEREMQDQFLDKMDLERERGITIKAQTVRLTYRADDGNDYILNLIDT
PGHVDFTYEVSRSLAACEGALLVVDASQGVEAQTLANVYLAIDNNLEVFPVLNKIDLPAAEPERVKHEIEEIIGLDAHDA
VMASAKEGIGTREILEEIVKKIPPPEGDPAAPLKALLFDSWYDQYQGVIILVRVIDGTVKKGDKIQLVSTGRSYEALKVG
VFAPVMREVPQLAAGEVGFLIAGIKDVADAKIGDTVTHALKPCVTPLGGFKEVKPMVFSGLYPIDTAQYEQLRDALAKLK
LNDSSFSFEPETSLALGFGFRCGFLGLLHMEIIQERLEREFNLDLITTAPTVVYKVHRIKGDVITIESANQLPPLQEIDY
IEEPFILASIHTPNEFVGGILSLCEEKRGVQREIKYLTPTRVMIIYELPLNEVVLDFYDRLKSITKGYASLDYEHLNYRR
SDLVRMNILINGEAVDALSLIIHRDKAYYRGRDLVSKMKELIPRQMFEVVIQAAIGAKVIARETVKALRKDVLAKCYGGD
ITRKRKLLEKQKEGKKRMKNVGNVELPQEAFLAILKVEEK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=606, Percent_Identity=52.4752475247525, Blast_Score=659, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=230, Percent_Identity=32.1739130434783, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI25306283, Length=153, Percent_Identity=45.0980392156863, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI25306287, Length=153, Percent_Identity=45.0980392156863, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI19923640, Length=153, Percent_Identity=45.0980392156863, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=40.9722222222222, Blast_Score=106, Evalue=7e-23,
Organism=Homo sapiens, GI18390331, Length=160, Percent_Identity=36.875, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=40.9090909090909, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=40.9090909090909, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=40.9090909090909, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI217272894, Length=160, Percent_Identity=34.375, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI217272892, Length=160, Percent_Identity=34.375, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI53729339, Length=277, Percent_Identity=25.2707581227437, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI53729337, Length=277, Percent_Identity=25.2707581227437, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI94966752, Length=193, Percent_Identity=29.5336787564767, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788922, Length=593, Percent_Identity=62.5632377740304, Blast_Score=739, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=502, Percent_Identity=28.8844621513944, Blast_Score=162, Evalue=7e-41,
Organism=Escherichia coli, GI1789738, Length=207, Percent_Identity=33.8164251207729, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790835, Length=151, Percent_Identity=31.7880794701987, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789559, Length=229, Percent_Identity=30.5676855895196, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=42.9738562091503, Blast_Score=503, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17556745, Length=460, Percent_Identity=27.3913043478261, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=39.7260273972603, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=156, Percent_Identity=37.1794871794872, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=132, Percent_Identity=37.1212121212121, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=158, Percent_Identity=34.1772151898734, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=133, Percent_Identity=36.8421052631579, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25141371, Length=300, Percent_Identity=27.3333333333333, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=48.0066445182724, Blast_Score=562, Evalue=1e-161,
Organism=Saccharomyces cerevisiae, GI6324707, Length=147, Percent_Identity=40.1360544217687, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=147, Percent_Identity=40.1360544217687, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=184, Percent_Identity=36.4130434782609, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=112, Percent_Identity=43.75, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6324166, Length=142, Percent_Identity=39.4366197183099, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324761, Length=281, Percent_Identity=29.8932384341637, Blast_Score=80, Evalue=9e-16,
Organism=Drosophila melanogaster, GI78706572, Length=604, Percent_Identity=46.0264900662252, Blast_Score=571, Evalue=1e-163,
Organism=Drosophila melanogaster, GI24582462, Length=161, Percent_Identity=39.1304347826087, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI28574573, Length=136, Percent_Identity=44.8529411764706, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=41.6107382550336, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=37.1621621621622, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=37.1621621621622, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=37.1621621621622, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21357743, Length=160, Percent_Identity=34.375, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921738, Length=278, Percent_Identity=26.6187050359712, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28572034, Length=223, Percent_Identity=30.9417040358744, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI281363316, Length=285, Percent_Identity=27.0175438596491, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17864358, Length=285, Percent_Identity=27.0175438596491, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_GEOSL (P60789)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952319.1
- ProteinModelPortal:   P60789
- SMR:   P60789
- GeneID:   2685825
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1266
- NMPDR:   fig|243231.1.peg.1257
- TIGR:   GSU1266
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- BioCyc:   GSUL243231:GSU_1266-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 67267; Mature: 67267

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDTIRNFSIIAHIDHGKSTLADRLLEYTGALTEREMQDQFLDKMDLERERGITIKAQT
CCCCCEECEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHCCCEEEEEE
VRLTYRADDGNDYILNLIDTPGHVDFTYEVSRSLAACEGALLVVDASQGVEAQTLANVYL
EEEEEEECCCCCEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHEEEEEE
AIDNNLEVFPVLNKIDLPAAEPERVKHEIEEIIGLDAHDAVMASAKEGIGTREILEEIVK
EECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
KIPPPEGDPAAPLKALLFDSWYDQYQGVIILVRVIDGTVKKGDKIQLVSTGRSYEALKVG
HCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCEEEH
VFAPVMREVPQLAAGEVGFLIAGIKDVADAKIGDTVTHALKPCVTPLGGFKEVKPMVFSG
HHHHHHHHHHHHHCCCHHHEECCHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCC
LYPIDTAQYEQLRDALAKLKLNDSSFSFEPETSLALGFGFRCGFLGLLHMEIIQERLERE
CCCCCHHHHHHHHHHHHHEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
FNLDLITTAPTVVYKVHRIKGDVITIESANQLPPLQEIDYIEEPFILASIHTPNEFVGGI
CCCEEEECCCHHHHHHEEECCCEEEEECCCCCCCHHHHCCCCCCEEEEEECCCHHHHHHH
LSLCEEKRGVQREIKYLTPTRVMIIYELPLNEVVLDFYDRLKSITKGYASLDYEHLNYRR
HHHHHHHHCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
SDLVRMNILINGEAVDALSLIIHRDKAYYRGRDLVSKMKELIPRQMFEVVIQAAIGAKVI
CCEEEEEEEECCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARETVKALRKDVLAKCYGGDITRKRKLLEKQKEGKKRMKNVGNVELPQEAFLAILKVEEK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEECCC
>Mature Secondary Structure
MKIDTIRNFSIIAHIDHGKSTLADRLLEYTGALTEREMQDQFLDKMDLERERGITIKAQT
CCCCCEECEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHCCCEEEEEE
VRLTYRADDGNDYILNLIDTPGHVDFTYEVSRSLAACEGALLVVDASQGVEAQTLANVYL
EEEEEEECCCCCEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHEEEEEE
AIDNNLEVFPVLNKIDLPAAEPERVKHEIEEIIGLDAHDAVMASAKEGIGTREILEEIVK
EECCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHH
KIPPPEGDPAAPLKALLFDSWYDQYQGVIILVRVIDGTVKKGDKIQLVSTGRSYEALKVG
HCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCEEEH
VFAPVMREVPQLAAGEVGFLIAGIKDVADAKIGDTVTHALKPCVTPLGGFKEVKPMVFSG
HHHHHHHHHHHHHCCCHHHEECCHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCC
LYPIDTAQYEQLRDALAKLKLNDSSFSFEPETSLALGFGFRCGFLGLLHMEIIQERLERE
CCCCCHHHHHHHHHHHHHEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
FNLDLITTAPTVVYKVHRIKGDVITIESANQLPPLQEIDYIEEPFILASIHTPNEFVGGI
CCCEEEECCCHHHHHHEEECCCEEEEECCCCCCCHHHHCCCCCCEEEEEECCCHHHHHHH
LSLCEEKRGVQREIKYLTPTRVMIIYELPLNEVVLDFYDRLKSITKGYASLDYEHLNYRR
HHHHHHHHCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
SDLVRMNILINGEAVDALSLIIHRDKAYYRGRDLVSKMKELIPRQMFEVVIQAAIGAKVI
CCEEEEEEEECCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARETVKALRKDVLAKCYGGDITRKRKLLEKQKEGKKRMKNVGNVELPQEAFLAILKVEEK
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA