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The map label for this gene is sppA-2 [C]

Identifier: 39996336

GI number: 39996336

Start: 1336399

End: 1337181

Strand: Direct

Name: sppA-2 [C]

Synonym: GSU1234

Alternate gene names: 39996336

Gene position: 1336399-1337181 (Clockwise)

Preceding gene: 39996334

Following gene: 39996348

Centisome position: 35.04

GC content: 62.84

Gene sequence:

>783_bases
ATGACGGCCGCAACCATCGGCGACGGGATTGGCTATGCGGAGGTGAAGGGGCCCATCATCGATTCCCAGGAAACCGTCAA
ACAGCTCGACGACCTTCGCAAGAAGAGCAGCGTCAAGGCAGTAGTGCTGAGGGTCGAGTCGCCGGGCGGGGTCATTGGCC
CGTCCCAGGAGATATACGCCGCGGTCAAGCGGTTGGCCGCCACCAAGAAGGTTGTTGTTTCCATGGGGAGCGTGGCGGCC
TCGGGCGGCTACCACGTCGCCGTGCCCGCTGCCGTGATCTATGCCAATCCGGGGACCATCACCGGCAGCATTGGCGTACT
CATGAAGCTCTCGAATATCGAAGGGCTCATGGACAAGGTGGGCCTCAAGGCATTTACCCTCAAGAGCGGCAAGTTCAAGG
ATTCCGGCTCTCCCGTGCGGAAGCTCACCGAGGAGGAGCGGGCCGTTCTTCAGGGGGTCATCGACAACCTTCACGATCAG
TTCGTACGTGCTGTGGCGGAGGGAAGACAGCTGCCGGTCGAGGAGGTGCGCCGGCTTGCCGACGGCAGGGTCTACACCGG
TGAGCAGGCCCTTCGTCTCAAGCTCGTGGACCGGCTCGGCACGCTGCACGATGCCGTAATGGAGGCAGGGCGTCTGGCTG
GAATCGAGGGAGAGCCGACCCTGATCATCCCCCCGAAAAAACGCAAGCTGCTGCGGGACATGCTCTTCGGCGAAGTGGCC
GAGGCGGTACGCGGGTCGGTGCGCAAGGAGGAGGGACTCAGCTTCAGCTACGAACTGGAGTGA

Upstream 100 bases:

>100_bases
CATGACCAGGAAACATGTTCTTATCGCCGGGGTAACCCTGGTTGCATTGATCATCCTCTTTACCTTTTCGGTGCAGATGG
CGCGGATTCTCATGGGAGAT

Downstream 100 bases:

>100_bases
GTCGTCTCTCTCTGATTTCATAAAAAAAGGGATGCCGCCATCGGGCGACGTCCCTTTTTCGTTTCCGGGTGAATTGCTGC
GTCAGCGCGGCATGGGGCAG

Product: signal peptide peptidase SppA, 36K type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTAATIGDGIGYAEVKGPIIDSQETVKQLDDLRKKSSVKAVVLRVESPGGVIGPSQEIYAAVKRLAATKKVVVSMGSVAA
SGGYHVAVPAAVIYANPGTITGSIGVLMKLSNIEGLMDKVGLKAFTLKSGKFKDSGSPVRKLTEEERAVLQGVIDNLHDQ
FVRAVAEGRQLPVEEVRRLADGRVYTGEQALRLKLVDRLGTLHDAVMEAGRLAGIEGEPTLIIPPKKRKLLRDMLFGEVA
EAVRGSVRKEEGLSFSYELE

Sequences:

>Translated_260_residues
MTAATIGDGIGYAEVKGPIIDSQETVKQLDDLRKKSSVKAVVLRVESPGGVIGPSQEIYAAVKRLAATKKVVVSMGSVAA
SGGYHVAVPAAVIYANPGTITGSIGVLMKLSNIEGLMDKVGLKAFTLKSGKFKDSGSPVRKLTEEERAVLQGVIDNLHDQ
FVRAVAEGRQLPVEEVRRLADGRVYTGEQALRLKLVDRLGTLHDAVMEAGRLAGIEGEPTLIIPPKKRKLLRDMLFGEVA
EAVRGSVRKEEGLSFSYELE
>Mature_259_residues
TAATIGDGIGYAEVKGPIIDSQETVKQLDDLRKKSSVKAVVLRVESPGGVIGPSQEIYAAVKRLAATKKVVVSMGSVAAS
GGYHVAVPAAVIYANPGTITGSIGVLMKLSNIEGLMDKVGLKAFTLKSGKFKDSGSPVRKLTEEERAVLQGVIDNLHDQF
VRAVAEGRQLPVEEVRRLADGRVYTGEQALRLKLVDRLGTLHDAVMEAGRLAGIEGEPTLIIPPKKRKLLRDMLFGEVAE
AVRGSVRKEEGLSFSYELE

Specific function: Digestion Of The Cleaved Signal Peptides. This Activity Is Necessary To Maintain Proper Secretion Of Mature Proteins Across The Membrane. [C]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=265, Percent_Identity=36.2264150943396, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1787527, Length=203, Percent_Identity=29.5566502463054, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 27834; Mature: 27702

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAATIGDGIGYAEVKGPIIDSQETVKQLDDLRKKSSVKAVVLRVESPGGVIGPSQEIYA
CCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHH
AVKRLAATKKVVVSMGSVAASGGYHVAVPAAVIYANPGTITGSIGVLMKLSNIEGLMDKV
HHHHHHHHHHHHHHHCCHHCCCCEEEEECEEEEEECCCCEECCHHHHEEHHHHHHHHHHH
GLKAFTLKSGKFKDSGSPVRKLTEEERAVLQGVIDNLHDQFVRAVAEGRQLPVEEVRRLA
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
DGRVYTGEQALRLKLVDRLGTLHDAVMEAGRLAGIEGEPTLIIPPKKRKLLRDMLFGEVA
CCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHH
EAVRGSVRKEEGLSFSYELE
HHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure 
TAATIGDGIGYAEVKGPIIDSQETVKQLDDLRKKSSVKAVVLRVESPGGVIGPSQEIYA
CCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHH
AVKRLAATKKVVVSMGSVAASGGYHVAVPAAVIYANPGTITGSIGVLMKLSNIEGLMDKV
HHHHHHHHHHHHHHHCCHHCCCCEEEEECEEEEEECCCCEECCHHHHEEHHHHHHHHHHH
GLKAFTLKSGKFKDSGSPVRKLTEEERAVLQGVIDNLHDQFVRAVAEGRQLPVEEVRRLA
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
DGRVYTGEQALRLKLVDRLGTLHDAVMEAGRLAGIEGEPTLIIPPKKRKLLRDMLFGEVA
CCCEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHH
EAVRGSVRKEEGLSFSYELE
HHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]