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The map label for this gene is greB [H]

Identifier: 39996303

GI number: 39996303

Start: 1304794

End: 1305279

Strand: Direct

Name: greB [H]

Synonym: GSU1201

Alternate gene names: 39996303

Gene position: 1304794-1305279 (Clockwise)

Preceding gene: 39996302

Following gene: 39996304

Centisome position: 34.21

GC content: 62.76

Gene sequence:

>486_bases
ATGGCAGGTCATTCCAACTACATCACTCCCGAGGGGGCGAAAAAACTCCGGACCGAGCTGACCTGGCTCTGGAAGGAGGA
GCGCCCCCGGGTCACCCAAGGTGTTGCCGATGCGGCCGCCGAGGGTGATCGATCTGAAAACGCCGAGTACATTTACGGTA
AGAAGCGGCTGCGGGAGATCGACCGCCGCATCCGCTTCCTTACTCAGCGGCTCGATGCCCTCACGGTTGTTGACGCTCCG
CCCCCTGCTACCGACAAGGTTTTTTTCGGTGCCTGGGTGCGGCTCGAAAACGAGGAGGGCGACGAGGTCGTCTACCGGGT
TGTCGGCCCGGACGAGACCGATGCCGCCAAAGGGCACATCAGTCTCGACTCCCCCATGGGCAAGTCACTGCTCGGTCGCC
GAGAGGGAGACGAGGTTCACGTCCGCCGTCCGGCTGGCGAAGCGGTCTTCACCGTACTCGAAGTCGGCTACCAGCCGCTG
GATTAA

Upstream 100 bases:

>100_bases
TTGCCGAATTCCGCGGTAAGGGCGCGGCGCAGACCAAGGATTCCATGGGAACGTTCGGCGACATTCTCAAGGCCCAGCTC
GACGACAAGAAGTAGGTCGG

Downstream 100 bases:

>100_bases
CAGGTTTTTCCCCGTCAGACGCAGGGATGAAGGGCCCGGCTAACCCCGGCTTCACCTCATTCCTGCGTCTTCGTCGTCAG
CTTGAATCTCCCGGAGGGCT

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MAGHSNYITPEGAKKLRTELTWLWKEERPRVTQGVADAAAEGDRSENAEYIYGKKRLREIDRRIRFLTQRLDALTVVDAP
PPATDKVFFGAWVRLENEEGDEVVYRVVGPDETDAAKGHISLDSPMGKSLLGRREGDEVHVRRPAGEAVFTVLEVGYQPL
D

Sequences:

>Translated_161_residues
MAGHSNYITPEGAKKLRTELTWLWKEERPRVTQGVADAAAEGDRSENAEYIYGKKRLREIDRRIRFLTQRLDALTVVDAP
PPATDKVFFGAWVRLENEEGDEVVYRVVGPDETDAAKGHISLDSPMGKSLLGRREGDEVHVRRPAGEAVFTVLEVGYQPL
D
>Mature_160_residues
AGHSNYITPEGAKKLRTELTWLWKEERPRVTQGVADAAAEGDRSENAEYIYGKKRLREIDRRIRFLTQRLDALTVVDAPP
PATDKVFFGAWVRLENEEGDEVVYRVVGPDETDAAKGHISLDSPMGKSLLGRREGDEVHVRRPAGEAVFTVLEVGYQPLD

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=150, Percent_Identity=53.3333333333333, Blast_Score=157, Evalue=3e-40,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=37.5, Blast_Score=98, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18091; Mature: 17959

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGHSNYITPEGAKKLRTELTWLWKEERPRVTQGVADAAAEGDRSENAEYIYGKKRLREI
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHH
DRRIRFLTQRLDALTVVDAPPPATDKVFFGAWVRLENEEGDEVVYRVVGPDETDAAKGHI
HHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCEE
SLDSPMGKSLLGRREGDEVHVRRPAGEAVFTVLEVGYQPLD
EECCCCCHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AGHSNYITPEGAKKLRTELTWLWKEERPRVTQGVADAAAEGDRSENAEYIYGKKRLREI
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHH
DRRIRFLTQRLDALTVVDAPPPATDKVFFGAWVRLENEEGDEVVYRVVGPDETDAAKGHI
HHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCEE
SLDSPMGKSLLGRREGDEVHVRRPAGEAVFTVLEVGYQPLD
EECCCCCHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]