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The map label for this gene is rhtB [C]

Identifier: 39996296

GI number: 39996296

Start: 1298743

End: 1299363

Strand: Direct

Name: rhtB [C]

Synonym: GSU1194

Alternate gene names: 39996296

Gene position: 1298743-1299363 (Clockwise)

Preceding gene: 39996295

Following gene: 39996297

Centisome position: 34.05

GC content: 63.93

Gene sequence:

>621_bases
ATGACAAATCTCAGTGTGTTCCTTGTTTCCGTTGTCCTCGTCACCGTTGCGCCGGGGCCCGATATCATCCAGGTTCTCAC
CCGGGGAGTAAGCCAGGGGCGGATGGCCGGCTTGGCTGCCGCGGCCGGTTTCAGCACCGGCTGTATCTTCCACACGACCC
TGGCGGCAGTGGGGGTGTCGGCCCTCATTCGGTCCTCCGACGTGGCATTCACCCTGATCAAGCTTGCCGGGGCCTGTTAT
CTGGTCTACATCGGCATCGGGGCGCTGCGGAACCGGGGGGCATCCCTGGTGGGCGAGCCGGCCGGCTTCGACGCCTTGGG
GGTCATCTACCGCCAGAGCATCGTGGCAAACATCCTCAATCCCAAGGTAACCCTCTTTTTTCTCGCGTTCCTGCCCCAGT
TCGTGTCCCCCGCGTCGGCCACTCCAGGCTTTCAGCTCATGGGGCTCGGCATCATCTTCATGGTCGTCACCATCATCATC
TTTGGCTCCGTGGCCCTTTTTGCGGGGCTCATCGGGGACTGGCTCCGACGTCGGCCTGCCGTTGCCGGACGGCTCCAGGC
CCTGGCCGGCGTTACCTTCATCGGCCTCGGCATCCGCGTGGCATTGCCCGATGTCCGCTGA

Upstream 100 bases:

>100_bases
AGGGCGCTGAAGGAAATTTCGACGATTAGTATCCCGCAGCCAGCGGCGTGAAGGGGGGGCAAAAGCCCCCCCTTTTTCCG
TTGCTGCTCCGGAGCAGACC

Downstream 100 bases:

>100_bases
CCCCTTCATCATAACCACCTTGGACGAGGAGGGGCTTGCTTCCCTCAAAAAGTGGTTCCTCGGCTACTGCCGCTCCTTTT
ACACCTCACGCGAAGAAGAC

Product: transporter, LysE family

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTNLSVFLVSVVLVTVAPGPDIIQVLTRGVSQGRMAGLAAAAGFSTGCIFHTTLAAVGVSALIRSSDVAFTLIKLAGACY
LVYIGIGALRNRGASLVGEPAGFDALGVIYRQSIVANILNPKVTLFFLAFLPQFVSPASATPGFQLMGLGIIFMVVTIII
FGSVALFAGLIGDWLRRRPAVAGRLQALAGVTFIGLGIRVALPDVR

Sequences:

>Translated_206_residues
MTNLSVFLVSVVLVTVAPGPDIIQVLTRGVSQGRMAGLAAAAGFSTGCIFHTTLAAVGVSALIRSSDVAFTLIKLAGACY
LVYIGIGALRNRGASLVGEPAGFDALGVIYRQSIVANILNPKVTLFFLAFLPQFVSPASATPGFQLMGLGIIFMVVTIII
FGSVALFAGLIGDWLRRRPAVAGRLQALAGVTFIGLGIRVALPDVR
>Mature_205_residues
TNLSVFLVSVVLVTVAPGPDIIQVLTRGVSQGRMAGLAAAAGFSTGCIFHTTLAAVGVSALIRSSDVAFTLIKLAGACYL
VYIGIGALRNRGASLVGEPAGFDALGVIYRQSIVANILNPKVTLFFLAFLPQFVSPASATPGFQLMGLGIIFMVVTIIIF
GSVALFAGLIGDWLRRRPAVAGRLQALAGVTFIGLGIRVALPDVR

Specific function: Conducts The Efflux Of Homoserine And Homoserine Lactone. [C]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=170, Percent_Identity=30.5882352941176, Blast_Score=90, Evalue=9e-20,
Organism=Escherichia coli, GI1788099, Length=203, Percent_Identity=25.1231527093596, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21361; Mature: 21230

Theoretical pI: Translated: 11.28; Mature: 11.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNLSVFLVSVVLVTVAPGPDIIQVLTRGVSQGRMAGLAAAAGFSTGCIFHTTLAAVGVS
CCCHHHHHHHHHHHHCCCCHHHHHHHHHCHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
ALIRSSDVAFTLIKLAGACYLVYIGIGALRNRGASLVGEPAGFDALGVIYRQSIVANILN
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
PKVTLFFLAFLPQFVSPASATPGFQLMGLGIIFMVVTIIIFGSVALFAGLIGDWLRRRPA
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VAGRLQALAGVTFIGLGIRVALPDVR
HHHHHHHHHHHHHHHCCCEEECCCCC
>Mature Secondary Structure 
TNLSVFLVSVVLVTVAPGPDIIQVLTRGVSQGRMAGLAAAAGFSTGCIFHTTLAAVGVS
CCHHHHHHHHHHHHCCCCHHHHHHHHHCHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
ALIRSSDVAFTLIKLAGACYLVYIGIGALRNRGASLVGEPAGFDALGVIYRQSIVANILN
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
PKVTLFFLAFLPQFVSPASATPGFQLMGLGIIFMVVTIIIFGSVALFAGLIGDWLRRRPA
CHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
VAGRLQALAGVTFIGLGIRVALPDVR
HHHHHHHHHHHHHHHCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8169201; 10984043 [H]