Click here to switch to the map view.

The map label for this gene is selU [H]

Identifier: 39996292

GI number: 39996292

Start: 1295588

End: 1296622

Strand: Reverse

Name: selU [H]

Synonym: GSU1190

Alternate gene names: 39996292

Gene position: 1296622-1295588 (Counterclockwise)

Preceding gene: 39996298

Following gene: 39996291

Centisome position: 34.0

GC content: 64.54

Gene sequence:

>1035_bases
GTGATCAAAACCACCCCGTTCCATGAATCGTTGCTCGACACCCACTTGGTGGTGGATGTCCGGACTCCCCTCGAATACGA
GGAGGATCACCTCCCCGGTGCCATCAATGTCCCGCTCCTCACCAACGAGGAACGGGTGGAGATCGGCACCATCTACAAGC
AGACCGGCCCCCTGGAGGCGCGCCGCCGGGGGCTGCACCTGACCGCTCACCGCTTTCCTGCCATGGTGGGGGAGATTGCC
CGGGCCGCGGCAGGCCGCCCCATTCTCGTCTACTGCTGGCGCGGGGGGCTCAGGAGCAAGACCGTCGTGTCGATCCTCGA
CCTGGCAGGGTTCGACGCGGTCCAGCTCCAGGGAGGCCACAAGGCCTTCCGCCACCTGGTTCTGTCATCATTCGACCCCT
TCACCCCCCCCGCGCCGCTGGTTGTCCTCCATGGCATGACCGGCGTGGGCAAGACGACGTTCCTCCTGCAGCTGGCACGG
GCCGGTCATGCCGTGGTCGACCTCGAGGGACTCGCCCGCCACCGGGGATCGGCCTTCGGCGAACTGGGACTTCGTCAGGA
CATCTCCCAGAAGCGGTTCGAAACTCTCCTCTGGGATGCCTTCCGCCGATTGCCTGCCGGCTGCCCCATCCTGCTGGAGG
GAGAAAGCAGGCGCATCGGCAGGCTCACTCTGCCGGGCAACCTCTACGACGTCATGCGGGGTAGTGTGAAGCTTTGGTGC
GAGGCGTCGGTGGCCACCCGGGTGGCCCGCCTCACGGAAGAGTACGGCCGCCCCGAATACCGGGACGGCATGGCCGGCGC
CCTGGAGCGGATCAGAAAAAAACTCGGCGGCGACAACTACGAAGCGATCAAAGGCCATCTGGACCGATGGGAAATGGAAC
CGTTCATGGAGGGCCTGATCCGGCATTACTATGACAAGCTCTACTACAAGACCCGCGACTGGGTGGAGGATGCCGTCATT
TCCCTGGAGGATTTTTCCTCAGGAGAGCGGGAGCTGAACACGTTCCTCGCCTCGCGCAGACAGGCCGCGGTGTAG

Upstream 100 bases:

>100_bases
GAGCGATTGCACCTCCGACCATCCCGCACGCGGTGGCGCACCATCCTTGCATTTGGCCCTGCTCCCCCCGTATAATGCTC
TGGTTTGCAAGGAGAATGAT

Downstream 100 bases:

>100_bases
AGCCATGCTGGCCCAGAGGTTGCGCAACCTCATCCAGTCCATGGTGCTCGTCACGGGCATGGTGGGGCTTCTTCTCACCC
TCGGCTGGCTCTTTGCCGGC

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MIKTTPFHESLLDTHLVVDVRTPLEYEEDHLPGAINVPLLTNEERVEIGTIYKQTGPLEARRRGLHLTAHRFPAMVGEIA
RAAAGRPILVYCWRGGLRSKTVVSILDLAGFDAVQLQGGHKAFRHLVLSSFDPFTPPAPLVVLHGMTGVGKTTFLLQLAR
AGHAVVDLEGLARHRGSAFGELGLRQDISQKRFETLLWDAFRRLPAGCPILLEGESRRIGRLTLPGNLYDVMRGSVKLWC
EASVATRVARLTEEYGRPEYRDGMAGALERIRKKLGGDNYEAIKGHLDRWEMEPFMEGLIRHYYDKLYYKTRDWVEDAVI
SLEDFSSGERELNTFLASRRQAAV

Sequences:

>Translated_344_residues
MIKTTPFHESLLDTHLVVDVRTPLEYEEDHLPGAINVPLLTNEERVEIGTIYKQTGPLEARRRGLHLTAHRFPAMVGEIA
RAAAGRPILVYCWRGGLRSKTVVSILDLAGFDAVQLQGGHKAFRHLVLSSFDPFTPPAPLVVLHGMTGVGKTTFLLQLAR
AGHAVVDLEGLARHRGSAFGELGLRQDISQKRFETLLWDAFRRLPAGCPILLEGESRRIGRLTLPGNLYDVMRGSVKLWC
EASVATRVARLTEEYGRPEYRDGMAGALERIRKKLGGDNYEAIKGHLDRWEMEPFMEGLIRHYYDKLYYKTRDWVEDAVI
SLEDFSSGERELNTFLASRRQAAV
>Mature_344_residues
MIKTTPFHESLLDTHLVVDVRTPLEYEEDHLPGAINVPLLTNEERVEIGTIYKQTGPLEARRRGLHLTAHRFPAMVGEIA
RAAAGRPILVYCWRGGLRSKTVVSILDLAGFDAVQLQGGHKAFRHLVLSSFDPFTPPAPLVVLHGMTGVGKTTFLLQLAR
AGHAVVDLEGLARHRGSAFGELGLRQDISQKRFETLLWDAFRRLPAGCPILLEGESRRIGRLTLPGNLYDVMRGSVKLWC
EASVATRVARLTEEYGRPEYRDGMAGALERIRKKLGGDNYEAIKGHLDRWEMEPFMEGLIRHYYDKLYYKTRDWVEDAVI
SLEDFSSGERELNTFLASRRQAAV

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=324, Percent_Identity=34.2592592592593, Blast_Score=142, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 38714; Mature: 38714

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTTPFHESLLDTHLVVDVRTPLEYEEDHLPGAINVPLLTNEERVEIGTIYKQTGPLEA
CCCCCCHHHHHHCCEEEEEECCCCCCCCCCCCCEEECCEECCCCCEEEEEHHHCCCCHHH
RRRGLHLTAHRFPAMVGEIARAAAGRPILVYCWRGGLRSKTVVSILDLAGFDAVQLQGGH
HHCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCHH
KAFRHLVLSSFDPFTPPAPLVVLHGMTGVGKTTFLLQLARAGHAVVDLEGLARHRGSAFG
HHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEHHHHHHHCCCHHH
ELGLRQDISQKRFETLLWDAFRRLPAGCPILLEGESRRIGRLTLPGNLYDVMRGSVKLWC
HCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCHHHHHCCCEEEEE
EASVATRVARLTEEYGRPEYRDGMAGALERIRKKLGGDNYEAIKGHLDRWEMEPFMEGLI
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHH
RHYYDKLYYKTRDWVEDAVISLEDFSSGERELNTFLASRRQAAV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKTTPFHESLLDTHLVVDVRTPLEYEEDHLPGAINVPLLTNEERVEIGTIYKQTGPLEA
CCCCCCHHHHHHCCEEEEEECCCCCCCCCCCCCEEECCEECCCCCEEEEEHHHCCCCHHH
RRRGLHLTAHRFPAMVGEIARAAAGRPILVYCWRGGLRSKTVVSILDLAGFDAVQLQGGH
HHCCCEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCHH
KAFRHLVLSSFDPFTPPAPLVVLHGMTGVGKTTFLLQLARAGHAVVDLEGLARHRGSAFG
HHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEHHHHHHHCCCHHH
ELGLRQDISQKRFETLLWDAFRRLPAGCPILLEGESRRIGRLTLPGNLYDVMRGSVKLWC
HCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCHHHHHCCCEEEEE
EASVATRVARLTEEYGRPEYRDGMAGALERIRKKLGGDNYEAIKGHLDRWEMEPFMEGLI
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHH
RHYYDKLYYKTRDWVEDAVISLEDFSSGERELNTFLASRRQAAV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]