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The map label for this gene is ogt [H]

Identifier: 39996275

GI number: 39996275

Start: 1278520

End: 1279068

Strand: Direct

Name: ogt [H]

Synonym: GSU1173

Alternate gene names: 39996275

Gene position: 1278520-1279068 (Clockwise)

Preceding gene: 39996274

Following gene: 39996278

Centisome position: 33.52

GC content: 65.21

Gene sequence:

>549_bases
TTGCTCGATGACGGGAACAGCGGCGCGCGACGAGGTGTATGTGTCCTTTTTCGAGACGGCGGTGGGGGTCGGTGCCGTGG
TGGCGGGCAGCGGGGGACTCAGGGAGGTGTTCCTCCCTTCAGGGTCGCCACGCGGGAGGATATGGTCGCGACCGTTCGCA
GCCGCTACCCCCGCCAGGCAGGCGAAAATTCTCTCAGTCGCGAAGCGGCTCGCCAGCTTGTTGCGTACTTTGCAGGTGAG
GCGGTTGAGTTCACGGTTCCTCTCGACGAGGAGCACGTCACGCCGTTTCGGAAAAAGGTGTACGAGATCGTACGCGGCAT
AGGGAGGGGGCAGGTCATGACCTATGGCCAGGTAGCCGTCGCCGCAGGCAGCCCCGGCGCTGCCCGGGGGGTTGGCTCGG
CCATGGCCGCCAATCCCCTTCCCATTATCGTTCCCTGTCATCGGGTCGTGGGCGTTGGGGGAGCCCTTACCGGCTATTCA
GGGGCCGGCGGGATCGACTCCAAGCGTTGGCTTCTAGAGCTGGAAGCAAGCACCGGGGCCGGAGAGTGA

Upstream 100 bases:

>100_bases
CTCGGCGCCGTGGGCGTGGGCATCGTGTTGTTTCTTGCAGCCGCCGCGTTGCTCCGCAGCGAAGAGACGCGCGAGCTGGC
GGCACAACTCAGAAGGAGGT

Downstream 100 bases:

>100_bases
AAAATCGGGCAGTTATGCACAGTCTCTCGATAGAATCTCCGTTTTTGTGAAGCCAGGGTTGAACGGTTGAGTGATAACTG
CCGGAAGTTCAAGGCTAACG

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MLDDGNSGARRGVCVLFRDGGGGRCRGGGQRGTQGGVPPFRVATREDMVATVRSRYPRQAGENSLSREAARQLVAYFAGE
AVEFTVPLDEEHVTPFRKKVYEIVRGIGRGQVMTYGQVAVAAGSPGAARGVGSAMAANPLPIIVPCHRVVGVGGALTGYS
GAGGIDSKRWLLELEASTGAGE

Sequences:

>Translated_182_residues
MLDDGNSGARRGVCVLFRDGGGGRCRGGGQRGTQGGVPPFRVATREDMVATVRSRYPRQAGENSLSREAARQLVAYFAGE
AVEFTVPLDEEHVTPFRKKVYEIVRGIGRGQVMTYGQVAVAAGSPGAARGVGSAMAANPLPIIVPCHRVVGVGGALTGYS
GAGGIDSKRWLLELEASTGAGE
>Mature_182_residues
MLDDGNSGARRGVCVLFRDGGGGRCRGGGQRGTQGGVPPFRVATREDMVATVRSRYPRQAGENSLSREAARQLVAYFAGE
AVEFTVPLDEEHVTPFRKKVYEIVRGIGRGQVMTYGQVAVAAGSPGAARGVGSAMAANPLPIIVPCHRVVGVGGALTGYS
GAGGIDSKRWLLELEASTGAGE

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=111, Percent_Identity=44.1441441441441, Blast_Score=81, Evalue=4e-16,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=49.5238095238095, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1788542, Length=135, Percent_Identity=35.5555555555556, Blast_Score=80, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=100, Percent_Identity=40, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115533068, Length=100, Percent_Identity=40, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=38.5542168674699, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18904; Mature: 18904

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDDGNSGARRGVCVLFRDGGGGRCRGGGQRGTQGGVPPFRVATREDMVATVRSRYPRQA
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCHHC
GENSLSREAARQLVAYFAGEAVEFTVPLDEEHVTPFRKKVYEIVRGIGRGQVMTYGQVAV
CCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHCCHHHHHHHHHHHCCCCCCEEEECEEEE
AAGSPGAARGVGSAMAANPLPIIVPCHRVVGVGGALTGYSGAGGIDSKRWLLELEASTGA
ECCCCCCHHCCCCHHHCCCCEEEEECHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
GE
CC
>Mature Secondary Structure
MLDDGNSGARRGVCVLFRDGGGGRCRGGGQRGTQGGVPPFRVATREDMVATVRSRYPRQA
CCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCHHC
GENSLSREAARQLVAYFAGEAVEFTVPLDEEHVTPFRKKVYEIVRGIGRGQVMTYGQVAV
CCCHHHHHHHHHHHHHHCCCEEEEEECCCHHHCCHHHHHHHHHHHCCCCCCEEEECEEEE
AAGSPGAARGVGSAMAANPLPIIVPCHRVVGVGGALTGYSGAGGIDSKRWLLELEASTGA
ECCCCCCHHCCCCHHHCCCCEEEEECHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
GE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]