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The map label for this gene is cwlS [H]

Identifier: 39995975

GI number: 39995975

Start: 925879

End: 926907

Strand: Direct

Name: cwlS [H]

Synonym: GSU0869

Alternate gene names: 39995975

Gene position: 925879-926907 (Clockwise)

Preceding gene: 39995974

Following gene: 39995976

Centisome position: 24.27

GC content: 59.18

Gene sequence:

>1029_bases
ATGAGTATACGATTGCGACTTACCATCTTCGTCCTCATCCTCTTAGCCTTACCCTCCTTGGCGCTTGCCGCCAAAAGCCA
TAAAGTCAAAAAGAACGAAACCCTTTCTTCCATTGCCCGCAAGTACCACGTCCGCGTCGCCGATCTCAAAGCAGCCAACA
ACCTTGCCAATACTCGCGTAAAGCCCGGCACCATCCTGGTTGTCCCTCCTCGTTCCGAGGCTGCCGCAGCGGAGCCCGTG
GCCGAGGTCGCTACCTACAAGGTGCGCAAGGGCGACACTCTGATCCGGATCGCCCGAAAGACCGGCGTGTCCGTGAATGA
TCTGCGACAGTTGAACAACCTCGGCAAGAAAAGGGTCAAGCCCGGCCAGGTACTGGCGCTTCGGGCCGTTGCCGACGAGG
AGCAACCGGCCAGGAAGCTCGCTCTCAAGAAGCCCCTCAAGAGCCTCGACATCTATAACGAAAGCGAATACGAACGCAGC
CTCGCGGAACTCGTGGGCGAGAGCGGCGAAGACACACAGGCCGATTTCACCAAAGGGGTAACCCTTGGCGGCGATGGCGT
AACCGAGCTGAAAAAGACAGCCTACAGTTTCATCGGCACCCGCTACCGCTTCGGCGGGACCACCCGCAAGGGGCTCGACT
GCTCCAGCTTTGTCCAGCACGTCTTCCGCGAGCTGGACGTAACCCTGCCCCGCACCGCCCGCGAACAGTTCCACGTGGGC
AACCCGGTCGCCACCGGAGACCTTCAGAAGGGCGATCTTCTCTTCTTTCATACCTATGCCCGCTTCCCTTCCCACGTGGG
CATCTATCTCGGCAACAACAAGATGATCCACGCCTCTTCACGGGACCGGCGAGTGGTCATCTCCTCAATCGATACCCCTT
ACTACCGTTCCCGCTTCATCGGCGCCAAGCGTATTTCGCAGATCAACCCCGACGTGCTTACGCTGGACGACCTGCTGACC
GGCGTGGAAGAGGAGGGGATCGAAGACATCCTTGCCAACGATACCCTCGGCGTTGGCCTGTTGAAGTAG

Upstream 100 bases:

>100_bases
CTGTATTTTTTAAGGAAAGAGTTTGACATTTGCGTGACAGTTTGTTATAAAAAGCAGGCTGCTTGCGTTATTTAATAAAA
CAATAGGGAAGGAGGGATAC

Downstream 100 bases:

>100_bases
CACCTGTCGGTCAATCATTCCAAAATCCGGTCCGTGGACCGGATTTTTTTTTGAGGCACCATGTACCCGCTCCTCGTCAC
CGCCGCCATTATCGAGCACA

Product: LysM domain/NLP/P60 family protein

Products: NA

Alternate protein names: Cell wall lytic enzyme associated with cell separation [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSIRLRLTIFVLILLALPSLALAAKSHKVKKNETLSSIARKYHVRVADLKAANNLANTRVKPGTILVVPPRSEAAAAEPV
AEVATYKVRKGDTLIRIARKTGVSVNDLRQLNNLGKKRVKPGQVLALRAVADEEQPARKLALKKPLKSLDIYNESEYERS
LAELVGESGEDTQADFTKGVTLGGDGVTELKKTAYSFIGTRYRFGGTTRKGLDCSSFVQHVFRELDVTLPRTAREQFHVG
NPVATGDLQKGDLLFFHTYARFPSHVGIYLGNNKMIHASSRDRRVVISSIDTPYYRSRFIGAKRISQINPDVLTLDDLLT
GVEEEGIEDILANDTLGVGLLK

Sequences:

>Translated_342_residues
MSIRLRLTIFVLILLALPSLALAAKSHKVKKNETLSSIARKYHVRVADLKAANNLANTRVKPGTILVVPPRSEAAAAEPV
AEVATYKVRKGDTLIRIARKTGVSVNDLRQLNNLGKKRVKPGQVLALRAVADEEQPARKLALKKPLKSLDIYNESEYERS
LAELVGESGEDTQADFTKGVTLGGDGVTELKKTAYSFIGTRYRFGGTTRKGLDCSSFVQHVFRELDVTLPRTAREQFHVG
NPVATGDLQKGDLLFFHTYARFPSHVGIYLGNNKMIHASSRDRRVVISSIDTPYYRSRFIGAKRISQINPDVLTLDDLLT
GVEEEGIEDILANDTLGVGLLK
>Mature_341_residues
SIRLRLTIFVLILLALPSLALAAKSHKVKKNETLSSIARKYHVRVADLKAANNLANTRVKPGTILVVPPRSEAAAAEPVA
EVATYKVRKGDTLIRIARKTGVSVNDLRQLNNLGKKRVKPGQVLALRAVADEEQPARKLALKKPLKSLDIYNESEYERSL
AELVGESGEDTQADFTKGVTLGGDGVTELKKTAYSFIGTRYRFGGTTRKGLDCSSFVQHVFRELDVTLPRTAREQFHVGN
PVATGDLQKGDLLFFHTYARFPSHVGIYLGNNKMIHASSRDRRVVISSIDTPYYRSRFIGAKRISQINPDVLTLDDLLTG
VEEEGIEDILANDTLGVGLLK

Specific function: Probably functions as a cell separation enzyme in addition to lytE and lytF [H]

COG id: COG0791

COG function: function code M; Cell wall-associated hydrolases (invasion-associated proteins)

Gene ontology:

Cell location: Cell surface. Note=Localized at cell separation sites and cell poles during late vegetative phase [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1788501, Length=110, Percent_Identity=45.4545454545455, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787944, Length=121, Percent_Identity=36.3636363636364, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1788001, Length=121, Percent_Identity=41.3223140495868, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786421, Length=113, Percent_Identity=39.8230088495575, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786405, Length=106, Percent_Identity=34.9056603773585, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000064
- InterPro:   IPR018392
- InterPro:   IPR002482 [H]

Pfam domain/function: PF01476 LysM; PF00877 NLPC_P60 [H]

EC number: =3.4.19.11 [H]

Molecular weight: Translated: 37691; Mature: 37560

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRLRLTIFVLILLALPSLALAAKSHKVKKNETLSSIARKYHVRVADLKAANNLANTRV
CCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEHHHHHHHHHCCCC
KPGTILVVPPRSEAAAAEPVAEVATYKVRKGDTLIRIARKTGVSVNDLRQLNNLGKKRVK
CCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHCCC
PGQVLALRAVADEEQPARKLALKKPLKSLDIYNESEYERSLAELVGESGEDTQADFTKGV
CCCEEEEEECCCCCHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCE
TLGGDGVTELKKTAYSFIGTRYRFGGTTRKGLDCSSFVQHVFRELDVTLPRTAREQFHVG
EECCCCHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCC
NPVATGDLQKGDLLFFHTYARFPSHVGIYLGNNKMIHASSRDRRVVISSIDTPYYRSRFI
CCCCCCCCCCCCEEEEEHHHCCCCCEEEEECCCEEEEECCCCCEEEEECCCCCHHHHHHH
GAKRISQINPDVLTLDDLLTGVEEEGIEDILANDTLGVGLLK
HHHHHHHCCCCEEEHHHHHHCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SIRLRLTIFVLILLALPSLALAAKSHKVKKNETLSSIARKYHVRVADLKAANNLANTRV
CEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEHHHHHHHHHCCCC
KPGTILVVPPRSEAAAAEPVAEVATYKVRKGDTLIRIARKTGVSVNDLRQLNNLGKKRVK
CCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHCCC
PGQVLALRAVADEEQPARKLALKKPLKSLDIYNESEYERSLAELVGESGEDTQADFTKGV
CCCEEEEEECCCCCHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHCCCCCCCHHHHHCCE
TLGGDGVTELKKTAYSFIGTRYRFGGTTRKGLDCSSFVQHVFRELDVTLPRTAREQFHVG
EECCCCHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCC
NPVATGDLQKGDLLFFHTYARFPSHVGIYLGNNKMIHASSRDRRVVISSIDTPYYRSRFI
CCCCCCCCCCCCEEEEEHHHCCCCCEEEEECCCEEEEECCCCCEEEEECCCCCHHHHHHH
GAKRISQINPDVLTLDDLLTGVEEEGIEDILANDTLGVGLLK
HHHHHHHCCCCEEEHHHHHHCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9734814; 9384377 [H]