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The map label for this gene is czcB [H]

Identifier: 39995935

GI number: 39995935

Start: 888914

End: 890185

Strand: Direct

Name: czcB [H]

Synonym: GSU0829

Alternate gene names: 39995935

Gene position: 888914-890185 (Clockwise)

Preceding gene: 39995934

Following gene: 39995936

Centisome position: 23.31

GC content: 64.39

Gene sequence:

>1272_bases
GTGAACAGGAAACGTGGGATCATTATCGGCCTTGTCCTGGCCATGGCCCTGGGGGGTGGTTTCTTCGCCTATCGTTCCAC
CCAGGTGACGGACGGCGCCGGCGGGCACACCGGCTCGAAGGATGCCGGTCACAAGGAGGAAAAGGCCGGTCACGACGGGG
AAAAGGGGCACGGCGAAGAGAACGGCGGCCATGACGGACATGGGGAGGAAGGCCGGATTTCAATGGCAGTCGAGGCACAG
AAGCAGAACGGCGTGGTCGTGGCCGCCGCCGCAGCACAGCGGCTTGCCGGCGTGATCAGCGCCACCGGCAAAGTGGAGGC
CAATGCCGACCGGACCGCCCATGTTTCGCCGCGCATTTCCGGCAAGATCGTCAGTGTCCGGGCCTCCCTCGGCGACAGCG
TCGCCGCCGGCCAGCCCCTTGTGACCCTGGACAGCGTCGAGCTGGGCGAAGCCCTGAACCGCTACCACCAGTCGAAGACG
CGGCTGGCCCTGGCCCAGTCCAACATGGACCGGATCAAGGCACTGGTGGAGAAGAAGATCGCGGCCCGCAAGGACATCCT
CCAGGCGGAGACCGACTACACAATGGCCCAGACCGAACTGCATACCGACGAGGAGCGCCTCGCCCTGTACGGCGTTTCGG
CGTCGGACCTCACAAACGCACGCCACAGGAAGCCGCTGCTGCCGGTCCGCTCTCCCATCGGCGGCGTCATCACTGAAAAA
CACGCCATCGTCGGCGAATTGGCCGATCCTTCAAAGAGCCTCTTTACCGTGGCCGACCTCTCGTCGGTCTGGGTGATGGT
GGACATCAACGAAAAGGACCTGGCTAAGGTCCACCGCGGGCAATCCGCCACCGTCACCCTTGGTGCGTTTCCCGACGTGA
AGCTGAAAGGGCGCATCACCTACATTGCCGATCTCGTCGACGTCAGCACCCGTACCGTCAAGGCCCGGGTCGAGGTACCC
AACCCGGCGCGGAAGCTGAAGCCGGAGATGTTCGCCACGGTGGAACTGACGCTGGCGGCCGACGCGCCGCCGGTCCTGGC
GGTCCCCGAGGATGCCCTGCAGGATCTGGACGGGAAAAAGGTCGTTTTCGTAGTGGAGAAGGATTCGGTATTCGCGGCCC
GTCCGGTACAGATCGGCCGGGCGGCCGGCGGCCTGGTGGAAATCGTTTCAGGGCTGAACGAGGGGGAACGCTATGCCGTC
AAGGGCTCGTTCATTCTGAAGTCGGAGCTGAAAAAGGGCGAGATGACGGACGAGCACGGCCACGGCGAATGA

Upstream 100 bases:

>100_bases
ACGGTTACCTTGTCGCCCTCGCCGAGCGGCAGGCGGCCCTGGCCCGGCTCGAAGCCTCTGTCGGGACCGATTTCCAGAAC
AGAACCAACGGAGGTGCGCA

Downstream 100 bases:

>100_bases
CCACCACCGACCATTACGTAAAGCGAGGTTCCGATGCTGGAGAAACTGATTGCCTATACGCTGCGCCAGAAGGGGATGGT
CATCTTCCTGGCGTTGCTGA

Product: CzcB family heavy metal efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MNRKRGIIIGLVLAMALGGGFFAYRSTQVTDGAGGHTGSKDAGHKEEKAGHDGEKGHGEENGGHDGHGEEGRISMAVEAQ
KQNGVVVAAAAAQRLAGVISATGKVEANADRTAHVSPRISGKIVSVRASLGDSVAAGQPLVTLDSVELGEALNRYHQSKT
RLALAQSNMDRIKALVEKKIAARKDILQAETDYTMAQTELHTDEERLALYGVSASDLTNARHRKPLLPVRSPIGGVITEK
HAIVGELADPSKSLFTVADLSSVWVMVDINEKDLAKVHRGQSATVTLGAFPDVKLKGRITYIADLVDVSTRTVKARVEVP
NPARKLKPEMFATVELTLAADAPPVLAVPEDALQDLDGKKVVFVVEKDSVFAARPVQIGRAAGGLVEIVSGLNEGERYAV
KGSFILKSELKKGEMTDEHGHGE

Sequences:

>Translated_423_residues
MNRKRGIIIGLVLAMALGGGFFAYRSTQVTDGAGGHTGSKDAGHKEEKAGHDGEKGHGEENGGHDGHGEEGRISMAVEAQ
KQNGVVVAAAAAQRLAGVISATGKVEANADRTAHVSPRISGKIVSVRASLGDSVAAGQPLVTLDSVELGEALNRYHQSKT
RLALAQSNMDRIKALVEKKIAARKDILQAETDYTMAQTELHTDEERLALYGVSASDLTNARHRKPLLPVRSPIGGVITEK
HAIVGELADPSKSLFTVADLSSVWVMVDINEKDLAKVHRGQSATVTLGAFPDVKLKGRITYIADLVDVSTRTVKARVEVP
NPARKLKPEMFATVELTLAADAPPVLAVPEDALQDLDGKKVVFVVEKDSVFAARPVQIGRAAGGLVEIVSGLNEGERYAV
KGSFILKSELKKGEMTDEHGHGE
>Mature_423_residues
MNRKRGIIIGLVLAMALGGGFFAYRSTQVTDGAGGHTGSKDAGHKEEKAGHDGEKGHGEENGGHDGHGEEGRISMAVEAQ
KQNGVVVAAAAAQRLAGVISATGKVEANADRTAHVSPRISGKIVSVRASLGDSVAAGQPLVTLDSVELGEALNRYHQSKT
RLALAQSNMDRIKALVEKKIAARKDILQAETDYTMAQTELHTDEERLALYGVSASDLTNARHRKPLLPVRSPIGGVITEK
HAIVGELADPSKSLFTVADLSSVWVMVDINEKDLAKVHRGQSATVTLGAFPDVKLKGRITYIADLVDVSTRTVKARVEVP
NPARKLKPEMFATVELTLAADAPPVLAVPEDALQDLDGKKVVFVVEKDSVFAARPVQIGRAAGGLVEIVSGLNEGERYAV
KGSFILKSELKKGEMTDEHGHGE

Specific function: CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete czc efflux system (CzcABC). The CzcB protein is thought to funnel zinc cations to the CzcA transport protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786787, Length=335, Percent_Identity=29.5522388059701, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081796, Length=319, Percent_Identity=25.705329153605, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789929, Length=308, Percent_Identity=27.5974025974026, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44880; Mature: 44880

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRKRGIIIGLVLAMALGGGFFAYRSTQVTDGAGGHTGSKDAGHKEEKAGHDGEKGHGEE
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCC
NGGHDGHGEEGRISMAVEAQKQNGVVVAAAAAQRLAGVISATGKVEANADRTAHVSPRIS
CCCCCCCCCCCEEEEEEEEECCCCEEEEHHHHHHHHHHHHCCCEEECCCCCEEECCCCCC
GKIVSVRASLGDSVAAGQPLVTLDSVELGEALNRYHQSKTRLALAQSNMDRIKALVEKKI
CEEEEEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AARKDILQAETDYTMAQTELHTDEERLALYGVSASDLTNARHRKPLLPVRSPIGGVITEK
HHHHHHHHCCCCCEEEHHHCCCCCCEEEEEECCHHHHCCHHHCCCCCCCCCCCCCCEECC
HAIVGELADPSKSLFTVADLSSVWVMVDINEKDLAKVHRGQSATVTLGAFPDVKLKGRIT
HHHEECCCCCCCCEEEEEECCEEEEEEECCHHHHHHHHCCCCCEEEEECCCCEEEECEEE
YIADLVDVSTRTVKARVEVPNPARKLKPEMFATVELTLAADAPPVLAVPEDALQDLDGKK
EEEHHHHCCCEEEEEEEECCCCHHHCCCCEEEEEEEEEECCCCCEEECCHHHHHCCCCCE
VVFVVEKDSVFAARPVQIGRAAGGLVEIVSGLNEGERYAVKGSFILKSELKKGEMTDEHG
EEEEEECCCEEEECCHHHCCCCCHHHHHHHCCCCCCEEEEECCEEEEHHCCCCCCCCCCC
HGE
CCC
>Mature Secondary Structure
MNRKRGIIIGLVLAMALGGGFFAYRSTQVTDGAGGHTGSKDAGHKEEKAGHDGEKGHGEE
CCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCC
NGGHDGHGEEGRISMAVEAQKQNGVVVAAAAAQRLAGVISATGKVEANADRTAHVSPRIS
CCCCCCCCCCCEEEEEEEEECCCCEEEEHHHHHHHHHHHHCCCEEECCCCCEEECCCCCC
GKIVSVRASLGDSVAAGQPLVTLDSVELGEALNRYHQSKTRLALAQSNMDRIKALVEKKI
CEEEEEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
AARKDILQAETDYTMAQTELHTDEERLALYGVSASDLTNARHRKPLLPVRSPIGGVITEK
HHHHHHHHCCCCCEEEHHHCCCCCCEEEEEECCHHHHCCHHHCCCCCCCCCCCCCCEECC
HAIVGELADPSKSLFTVADLSSVWVMVDINEKDLAKVHRGQSATVTLGAFPDVKLKGRIT
HHHEECCCCCCCCEEEEEECCEEEEEEECCHHHHHHHHCCCCCEEEEECCCCEEEECEEE
YIADLVDVSTRTVKARVEVPNPARKLKPEMFATVELTLAADAPPVLAVPEDALQDLDGKK
EEEHHHHCCCEEEEEEEECCCCHHHCCCCEEEEEEEEEECCCCCEEECCHHHHHCCCCCE
VVFVVEKDSVFAARPVQIGRAAGGLVEIVSGLNEGERYAVKGSFILKSELKKGEMTDEHG
EEEEEECCCEEEECCHHHCCCCCHHHHHHHCCCCCCEEEEECCEEEEHHCCCCCCCCCCC
HGE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8829543 [H]