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The map label for this gene is hycD [H]

Identifier: 39995846

GI number: 39995846

Start: 786034

End: 786942

Strand: Direct

Name: hycD [H]

Synonym: GSU0740

Alternate gene names: 39995846

Gene position: 786034-786942 (Clockwise)

Preceding gene: 39995845

Following gene: 39995847

Centisome position: 20.61

GC content: 66.23

Gene sequence:

>909_bases
ATGCTCGACATCATCATCCATCTGCTGCTGGCGATCCTGATGCCCCCCCTGCTCCTGGGGGTGATCGTCAAGACCAAGGC
CGCCTTTGCGGGCCGGGTCGGGGCGCCGCTTCTGCAGCCATACTATGACATTGCGCGGCTGCTGCGCAAGGGGAGCGTGT
TCAGCGATACCACCACCTGGGTGTTCCGTGCCGGGCCGGTGGTGACCCTGGCCGCCACCGCCGTGGCAGCGCTGCTGGTC
CCTTTGGGCAACCATCCGGCGCCGGTCTCCTTTGCCGGAGACATGATCCTCTTTGCCTACCTCTTCGGTCTGGCCCGGTT
CTTCACCACCGTGGCCGCCCTGGACACCGGCTCCAGCTTCGAGGGGATGGGGGCGGCCCGGGAGGTGACCTTTTCCTGTC
TGGCCGAGCCGACCCTCTTCTTCGCCCTCATCACCCTGGCCCGCATGAGCGGCAGCCTGTCGCTTACCCCCATGCTCACC
CACGCCACCGTGGGGGATTGGCTCACCGCCGGCGCCTCCCTGCTGCTGCTGGTGGGGGCGCTGTTTCTGGTTCTCCTGGT
GGAGAACTGCCGCATCCCCTTTGACGACCCCACCACCCACCTGGAACTGACCATGATCCACGAGGTGATGGTGCTGGACC
ACAGCGGCCCCGCCTTCGGCCTGATCCTCTACGGCGCGGCCCTCAAGCTCTTCGTGCTCGGGGCCTTCTTCATGAACGTG
GCCCTTCCCGTGCGGACCGGCAACACCCTGGCCGACTGGGGGATCTTCGTGGCATCCATGCTGGTGCTGGCGGTGGCCGT
GGGGGTGGTGGAATCGGTCATGGCCCGGCTGCGCCTGATCCGCATCCCGCAACTGCTTGTGGCGGCGACAATCCTCTCCG
CCTTTTCCATGCTCCTGATCCTGAGGTAA

Upstream 100 bases:

>100_bases
GCGGCGGCTGCAGCACGGCCGGCTGCACCTCTACATGCTCTACATCCTGACCACGCTCTTTCTGCTGATGGTCTGGGCCC
ACTGACGGGGAGGGCGCGCC

Downstream 100 bases:

>100_bases
CCCATGGTTTCCCTTGCCGACCAACTGCTGGTGCTGGTCATGCTGATCAACTTCGTCGTCCTGGGGTCGAGCCGGATGGC
CTTCTGCATCCGGGCCGCCG

Product: NAD-dependent dehydrogenase subunit

Products: NA

Alternate protein names: FHL subunit 4; Hydrogenase 3 component D [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MLDIIIHLLLAILMPPLLLGVIVKTKAAFAGRVGAPLLQPYYDIARLLRKGSVFSDTTTWVFRAGPVVTLAATAVAALLV
PLGNHPAPVSFAGDMILFAYLFGLARFFTTVAALDTGSSFEGMGAAREVTFSCLAEPTLFFALITLARMSGSLSLTPMLT
HATVGDWLTAGASLLLLVGALFLVLLVENCRIPFDDPTTHLELTMIHEVMVLDHSGPAFGLILYGAALKLFVLGAFFMNV
ALPVRTGNTLADWGIFVASMLVLAVAVGVVESVMARLRLIRIPQLLVAATILSAFSMLLILR

Sequences:

>Translated_302_residues
MLDIIIHLLLAILMPPLLLGVIVKTKAAFAGRVGAPLLQPYYDIARLLRKGSVFSDTTTWVFRAGPVVTLAATAVAALLV
PLGNHPAPVSFAGDMILFAYLFGLARFFTTVAALDTGSSFEGMGAAREVTFSCLAEPTLFFALITLARMSGSLSLTPMLT
HATVGDWLTAGASLLLLVGALFLVLLVENCRIPFDDPTTHLELTMIHEVMVLDHSGPAFGLILYGAALKLFVLGAFFMNV
ALPVRTGNTLADWGIFVASMLVLAVAVGVVESVMARLRLIRIPQLLVAATILSAFSMLLILR
>Mature_302_residues
MLDIIIHLLLAILMPPLLLGVIVKTKAAFAGRVGAPLLQPYYDIARLLRKGSVFSDTTTWVFRAGPVVTLAATAVAALLV
PLGNHPAPVSFAGDMILFAYLFGLARFFTTVAALDTGSSFEGMGAAREVTFSCLAEPTLFFALITLARMSGSLSLTPMLT
HATVGDWLTAGASLLLLVGALFLVLLVENCRIPFDDPTTHLELTMIHEVMVLDHSGPAFGLILYGAALKLFVLGAFFMNV
ALPVRTGNTLADWGIFVASMLVLAVAVGVVESVMARLRLIRIPQLLVAATILSAFSMLLILR

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG0650

COG function: function code C; Formate hydrogenlyase subunit 4

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789077, Length=288, Percent_Identity=32.9861111111111, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI87082115, Length=240, Percent_Identity=31.25, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001694
- InterPro:   IPR018086 [H]

Pfam domain/function: PF00146 NADHdh [H]

EC number: NA

Molecular weight: Translated: 32195; Mature: 32195

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDIIIHLLLAILMPPLLLGVIVKTKAAFAGRVGAPLLQPYYDIARLLRKGSVFSDTTTW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCE
VFRAGPVVTLAATAVAALLVPLGNHPAPVSFAGDMILFAYLFGLARFFTTVAALDTGSSF
EEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EGMGAAREVTFSCLAEPTLFFALITLARMSGSLSLTPMLTHATVGDWLTAGASLLLLVGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
LFLVLLVENCRIPFDDPTTHLELTMIHEVMVLDHSGPAFGLILYGAALKLFVLGAFFMNV
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHH
ALPVRTGNTLADWGIFVASMLVLAVAVGVVESVMARLRLIRIPQLLVAATILSAFSMLLI
HCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LR
CC
>Mature Secondary Structure
MLDIIIHLLLAILMPPLLLGVIVKTKAAFAGRVGAPLLQPYYDIARLLRKGSVFSDTTTW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCE
VFRAGPVVTLAATAVAALLVPLGNHPAPVSFAGDMILFAYLFGLARFFTTVAALDTGSSF
EEECCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EGMGAAREVTFSCLAEPTLFFALITLARMSGSLSLTPMLTHATVGDWLTAGASLLLLVGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
LFLVLLVENCRIPFDDPTTHLELTMIHEVMVLDHSGPAFGLILYGAALKLFVLGAFFMNV
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHH
ALPVRTGNTLADWGIFVASMLVLAVAVGVVESVMARLRLIRIPQLLVAATILSAFSMLLI
HCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503 [H]