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The map label for this gene is hyfB [H]

Identifier: 39995845

GI number: 39995845

Start: 784033

End: 786018

Strand: Direct

Name: hyfB [H]

Synonym: GSU0739

Alternate gene names: 39995845

Gene position: 784033-786018 (Clockwise)

Preceding gene: 39995844

Following gene: 39995846

Centisome position: 20.56

GC content: 68.33

Gene sequence:

>1986_bases
ATGGAATCCGCGTTAGAAGGGGCGGACCCCGCCTCTCAGATTCTTGCCGCCATAGTCCTGGTGGCCCTTTCCGGCGTTCC
GGGCGTGCTGGCGCGCCGATTCGTGCCGGGCCAGATCCTGGCCGCCGTCATGGCTGTGGCTGCGTCTCTGCTTGGCTTGA
CCGGCACGATTCGGACGCTGCTGGAGCACCGGACAACGGCCTTCAGTCTTGCCTGGACCCTCCCCTTCGGCCCCGCCGAC
CTGGCCGTCGATCCCCTGTCGGCTCTCTTCCTGATTCCCGTCTTTCTCGTCTTCGGCTGCGGCTCCCTCTATGCTCTCGG
CTACTGGCCCGCCGCTGCGAACCGTTCCACCGAGCCGGCCCTTACGTTCTTCTACGGGATTCTGGCCGCGGCCATGGCGG
GCCTGGTGGTGGCCCGCAACGGGGTTCTCTTCCTCATGGCGTGGGAGGCTATGGCCCTGTCGGCCTGGTTCCTCCTCGCC
ACCGAGCACCGTGACCCGGCCGTGCGCCAGGCGGGGATCGTCTATCTGGCGGCGACCCATGCCGGCACCGCGGCGCTCTT
CGTGCTCTTCTGCGTCCTGCGGGGCGTCACCGGATCCTTCGTGTTTCCCGCGCCACACAGCCTCGACCCGGCCTTGGTGC
CGGCCACGGTCCTGTTTCTGGCCGCCCTTGCCGGGTTCGGCGCCAAGGCCGGGATCATGCCGCTCCACATCTGGCTGCCC
GCGGCCCATGCCAGCGCGCCGAGCCACGTCTCCGCCCTCATGTCGGGGGTCATGCTCAAGATGGGGCTCTACGGGCTGGT
GCGGACCATCTCGTTTTTCGCGCCGGTACCCCTCTGGTGGGGCGGTCTCATTCTGGTGCTGGGGGCGGTTTCGGCCCTGG
CCGGCATCGTCCTGGCGGCGTCCCAGCGCGATCTGAAACGGCTGCTGGCCTGCAGCAGCATCGAGAACATCGGCATCATC
GCCTTGGGCATCGGTACGGCCCTGGTGGGGGAAGCCACTGGCAACGTCCCCCTCTATACCCTCGGCATGACCGGGGCACT
GCTCCACCTGCTCAATCACAGCCTGTTCAAGCCGCTCCTCTTTTTCGGGGCCGGCGCGGTGATCCATGCCACCGGCACCC
GGGAGATGGACCGAATGGGAGGGCTTGCCCGCCTCATGCCCCGGAGCGCGCTCTTCTTCCTGGTGGGGTCCGTGGCCATC
TGCGGGCTGCCTCCCCTGAACGGCTTCGTGGGGGAATTTCTGCTCTATGCCGGCTTCTTCGGCGAGGCCGTGGCTGACCC
GGTGCCGCTCCTGGCCCTGTCGGCGCCGCTTCTGGCCGTGGTGGGGGGCATCGCGACGGTCTCCTTCGTGAAGCTCTACG
GCGTGATTTTTCTGGGGGTTCCCCGCTGCGGCGCGCATCATCACTCCCACCTGCCCGACTGGCGGATGGGCGCGCCCATG
GGGCTGCTGGCCCTGCTCTGCCTCGCGGCGGGGATCGCGCCCGGGCCGGTGCTGAGCCTGGTGGAGCCGGCAGTGGGGGC
CTTCGGCATGGTTCCCCCGGCCCCCCTCGAAACCCTCGTTCCCCTGGGGTGGCTCACCCTGGGGGCGGGCATGCTGGGCG
CTGCGGTGATCCTCTCGGGGCTGCTCCTGATGTTCCGGCTGAAACGGACCCCGGTCGCGCGGGGTACTACCTGGGGGTGC
GGCTACCCGGCCCCGTCGCCGCGGATGCAGTACACGGGAGTTTCCTTTGCCGATTCCCTGGTGAGGCAGTTCGAAGGGGT
CGTCGCCCCCCGCCGGGAAGGTCCGGCCGTGGCGGGATTCTTCCCGGCACCGGTTCTGTTCCGGTTCGTGCCTACTGAAA
CGATCCTTGAGCGGGTCATCATGCCGCTGTTCAGGGTCGCGGGGTTTTCCTTCGCCTTCCTGCGGCGGCTGCAGCACGGC
CGGCTGCACCTCTACATGCTCTACATCCTGACCACGCTCTTTCTGCTGATGGTCTGGGCCCACTGA

Upstream 100 bases:

>100_bases
TGTTATTGACGCATAAAAAATAGTACCATATAGTTTTATAAGGTGTTAGCGTTTTTTGTGTGTTTTGCTGTTTCTATGTG
TCGTTAGTGGGTGGAAATCC

Downstream 100 bases:

>100_bases
CGGGGAGGGCGCGCCATGCTCGACATCATCATCCATCTGCTGCTGGCGATCCTGATGCCCCCCCTGCTCCTGGGGGTGAT
CGTCAAGACCAAGGCCGCCT

Product: NAD-dependent dehydrogenase subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 661; Mature: 661

Protein sequence:

>661_residues
MESALEGADPASQILAAIVLVALSGVPGVLARRFVPGQILAAVMAVAASLLGLTGTIRTLLEHRTTAFSLAWTLPFGPAD
LAVDPLSALFLIPVFLVFGCGSLYALGYWPAAANRSTEPALTFFYGILAAAMAGLVVARNGVLFLMAWEAMALSAWFLLA
TEHRDPAVRQAGIVYLAATHAGTAALFVLFCVLRGVTGSFVFPAPHSLDPALVPATVLFLAALAGFGAKAGIMPLHIWLP
AAHASAPSHVSALMSGVMLKMGLYGLVRTISFFAPVPLWWGGLILVLGAVSALAGIVLAASQRDLKRLLACSSIENIGII
ALGIGTALVGEATGNVPLYTLGMTGALLHLLNHSLFKPLLFFGAGAVIHATGTREMDRMGGLARLMPRSALFFLVGSVAI
CGLPPLNGFVGEFLLYAGFFGEAVADPVPLLALSAPLLAVVGGIATVSFVKLYGVIFLGVPRCGAHHHSHLPDWRMGAPM
GLLALLCLAAGIAPGPVLSLVEPAVGAFGMVPPAPLETLVPLGWLTLGAGMLGAAVILSGLLLMFRLKRTPVARGTTWGC
GYPAPSPRMQYTGVSFADSLVRQFEGVVAPRREGPAVAGFFPAPVLFRFVPTETILERVIMPLFRVAGFSFAFLRRLQHG
RLHLYMLYILTTLFLLMVWAH

Sequences:

>Translated_661_residues
MESALEGADPASQILAAIVLVALSGVPGVLARRFVPGQILAAVMAVAASLLGLTGTIRTLLEHRTTAFSLAWTLPFGPAD
LAVDPLSALFLIPVFLVFGCGSLYALGYWPAAANRSTEPALTFFYGILAAAMAGLVVARNGVLFLMAWEAMALSAWFLLA
TEHRDPAVRQAGIVYLAATHAGTAALFVLFCVLRGVTGSFVFPAPHSLDPALVPATVLFLAALAGFGAKAGIMPLHIWLP
AAHASAPSHVSALMSGVMLKMGLYGLVRTISFFAPVPLWWGGLILVLGAVSALAGIVLAASQRDLKRLLACSSIENIGII
ALGIGTALVGEATGNVPLYTLGMTGALLHLLNHSLFKPLLFFGAGAVIHATGTREMDRMGGLARLMPRSALFFLVGSVAI
CGLPPLNGFVGEFLLYAGFFGEAVADPVPLLALSAPLLAVVGGIATVSFVKLYGVIFLGVPRCGAHHHSHLPDWRMGAPM
GLLALLCLAAGIAPGPVLSLVEPAVGAFGMVPPAPLETLVPLGWLTLGAGMLGAAVILSGLLLMFRLKRTPVARGTTWGC
GYPAPSPRMQYTGVSFADSLVRQFEGVVAPRREGPAVAGFFPAPVLFRFVPTETILERVIMPLFRVAGFSFAFLRRLQHG
RLHLYMLYILTTLFLLMVWAH
>Mature_661_residues
MESALEGADPASQILAAIVLVALSGVPGVLARRFVPGQILAAVMAVAASLLGLTGTIRTLLEHRTTAFSLAWTLPFGPAD
LAVDPLSALFLIPVFLVFGCGSLYALGYWPAAANRSTEPALTFFYGILAAAMAGLVVARNGVLFLMAWEAMALSAWFLLA
TEHRDPAVRQAGIVYLAATHAGTAALFVLFCVLRGVTGSFVFPAPHSLDPALVPATVLFLAALAGFGAKAGIMPLHIWLP
AAHASAPSHVSALMSGVMLKMGLYGLVRTISFFAPVPLWWGGLILVLGAVSALAGIVLAASQRDLKRLLACSSIENIGII
ALGIGTALVGEATGNVPLYTLGMTGALLHLLNHSLFKPLLFFGAGAVIHATGTREMDRMGGLARLMPRSALFFLVGSVAI
CGLPPLNGFVGEFLLYAGFFGEAVADPVPLLALSAPLLAVVGGIATVSFVKLYGVIFLGVPRCGAHHHSHLPDWRMGAPM
GLLALLCLAAGIAPGPVLSLVEPAVGAFGMVPPAPLETLVPLGWLTLGAGMLGAAVILSGLLLMFRLKRTPVARGTTWGC
GYPAPSPRMQYTGVSFADSLVRQFEGVVAPRREGPAVAGFFPAPVLFRFVPTETILERVIMPLFRVAGFSFAFLRRLQHG
RLHLYMLYILTTLFLLMVWAH

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=357, Percent_Identity=27.7310924369748, Blast_Score=94, Evalue=4e-19,
Organism=Homo sapiens, GI251831116, Length=201, Percent_Identity=31.8407960199005, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1788827, Length=614, Percent_Identity=37.4592833876222, Blast_Score=311, Evalue=7e-86,
Organism=Escherichia coli, GI2367154, Length=524, Percent_Identity=34.5419847328244, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI1788831, Length=360, Percent_Identity=34.4444444444444, Blast_Score=145, Evalue=1e-35,
Organism=Escherichia coli, GI1788829, Length=400, Percent_Identity=29.75, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1788613, Length=383, Percent_Identity=28.9817232375979, Blast_Score=112, Evalue=8e-26,
Organism=Escherichia coli, GI1788614, Length=367, Percent_Identity=29.4277929155313, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI145693160, Length=423, Percent_Identity=24.3498817966903, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 69368; Mature: 69368

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESALEGADPASQILAAIVLVALSGVPGVLARRFVPGQILAAVMAVAASLLGLTGTIRTL
CCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LEHRTTAFSLAWTLPFGPADLAVDPLSALFLIPVFLVFGCGSLYALGYWPAAANRSTEPA
HHHCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCCCCHH
LTFFYGILAAAMAGLVVARNGVLFLMAWEAMALSAWFLLATEHRDPAVRQAGIVYLAATH
HHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHEEECCCCCHHHHCCEEEEEECC
AGTAALFVLFCVLRGVTGSFVFPAPHSLDPALVPATVLFLAALAGFGAKAGIMPLHIWLP
CHHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
AAHASAPSHVSALMSGVMLKMGLYGLVRTISFFAPVPLWWGGLILVLGAVSALAGIVLAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SQRDLKRLLACSSIENIGIIALGIGTALVGEATGNVPLYTLGMTGALLHLLNHSLFKPLL
HHHHHHHHHHHHCCCCCCEEEEEHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHH
FFGAGAVIHATGTREMDRMGGLARLMPRSALFFLVGSVAICGLPPLNGFVGEFLLYAGFF
HHCCCCEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GEAVADPVPLLALSAPLLAVVGGIATVSFVKLYGVIFLGVPRCGAHHHSHLPDWRMGAPM
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCH
GLLALLCLAAGIAPGPVLSLVEPAVGAFGMVPPAPLETLVPLGWLTLGAGMLGAAVILSG
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLLMFRLKRTPVARGTTWGCGYPAPSPRMQYTGVSFADSLVRQFEGVVAPRREGPAVAGF
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCCCCCCCCCEECC
FPAPVLFRFVPTETILERVIMPLFRVAGFSFAFLRRLQHGRLHLYMLYILTTLFLLMVWA
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
H
C
>Mature Secondary Structure
MESALEGADPASQILAAIVLVALSGVPGVLARRFVPGQILAAVMAVAASLLGLTGTIRTL
CCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LEHRTTAFSLAWTLPFGPADLAVDPLSALFLIPVFLVFGCGSLYALGYWPAAANRSTEPA
HHHCCHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCCCCHH
LTFFYGILAAAMAGLVVARNGVLFLMAWEAMALSAWFLLATEHRDPAVRQAGIVYLAATH
HHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHEEECCCCCHHHHCCEEEEEECC
AGTAALFVLFCVLRGVTGSFVFPAPHSLDPALVPATVLFLAALAGFGAKAGIMPLHIWLP
CHHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
AAHASAPSHVSALMSGVMLKMGLYGLVRTISFFAPVPLWWGGLILVLGAVSALAGIVLAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SQRDLKRLLACSSIENIGIIALGIGTALVGEATGNVPLYTLGMTGALLHLLNHSLFKPLL
HHHHHHHHHHHHCCCCCCEEEEEHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHH
FFGAGAVIHATGTREMDRMGGLARLMPRSALFFLVGSVAICGLPPLNGFVGEFLLYAGFF
HHCCCCEEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GEAVADPVPLLALSAPLLAVVGGIATVSFVKLYGVIFLGVPRCGAHHHSHLPDWRMGAPM
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCH
GLLALLCLAAGIAPGPVLSLVEPAVGAFGMVPPAPLETLVPLGWLTLGAGMLGAAVILSG
HHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLLMFRLKRTPVARGTTWGCGYPAPSPRMQYTGVSFADSLVRQFEGVVAPRREGPAVAGF
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCCCCCCCCCEECC
FPAPVLFRFVPTETILERVIMPLFRVAGFSFAFLRRLQHGRLHLYMLYILTTLFLLMVWA
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
H
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]