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The map label for this gene is thiG

Identifier: 39995695

GI number: 39995695

Start: 621450

End: 622232

Strand: Reverse

Name: thiG

Synonym: GSU0588

Alternate gene names: 39995695

Gene position: 622232-621450 (Counterclockwise)

Preceding gene: 39995696

Following gene: 39995694

Centisome position: 16.31

GC content: 60.79

Gene sequence:

>783_bases
ATGTCCAATGCAGCCGATAAACTGGTCATCGCCGGCCGCGAGTTTTCCTCCCGCCTCATGGTCGGCACCGGCAAGTACGC
CAGCAACGAGCAGATGATCAAGGCCCTGGAGGTTTCAGGCGCAGAAATCATTACGGTGGCGGTACGTCGGGTGAATCTGG
CCGACCGGGGCAAAGGGTGCCTGCTTGACTTCATCGATCCGAAAAAGTACACGCTCCTGCCCAACACCGCCGGCTGCTAC
ACGGCCGATGACGCGGTCAGAACCTGCCGACTGGCCCGGGAGGCCGGCATGAGCGATCTGGTGAAGCTGGAGGTGCTCGG
CGACGAGAAGACCCTCTTCCCTGACAATGAAGAACTGCTCAAGGCAGCAAAAATCTTGGTGAAGGAAGGGTTTACCGTCC
TTCCCTACACCAGCGACGATCCCATTGTCTGCAAAAAGCTTGAAGATATCGGCTGTGCCGCGGTCATGCCGCTGGGCGCC
CCCATCGGCAGCGGCCTGGGCATTCGGAATCCGTACAACATCCTGATCATCAAGGAAACGGTCAAGGTGCCGGTCATCGT
CGATGCCGGTGTCGGCACCGCCTCCGACGCCGCCATCGCCATGGAGTTGGGCATCGACGGAGTTCTCATGAATACCGGCA
TCGCCGGCGCCAGGGATCCCATCGCCATGGCAGAGGCAATGAACATGGCGGTGCGGGCCGGCCGGCTCGCCTACCTGGCG
GGACGCATTCCCAAGAAACTCTATGCCACGGCATCGAGCCCCATCGAGGGGATGATTGGCTAA

Upstream 100 bases:

>100_bases
AAATCGTCCACTTCGTAGGTGGCGGGTAAGGTCGGCCGCATCGGTCCGCCCCAGCGCCCGACAATGCGTCGCGATTCAAA
CACTTCAAGGAGATTACACC

Downstream 100 bases:

>100_bases
GGTCGATTTTTCGCTCTACCTGATCACCGACCGCCACCAGGCTGGTGGCAGGGACCTGCTCGCCGTGGTCGAGGGAGCCC
TGGCCGGTGGCGTGCGGTGC

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSNAADKLVIAGREFSSRLMVGTGKYASNEQMIKALEVSGAEIITVAVRRVNLADRGKGCLLDFIDPKKYTLLPNTAGCY
TADDAVRTCRLAREAGMSDLVKLEVLGDEKTLFPDNEELLKAAKILVKEGFTVLPYTSDDPIVCKKLEDIGCAAVMPLGA
PIGSGLGIRNPYNILIIKETVKVPVIVDAGVGTASDAAIAMELGIDGVLMNTGIAGARDPIAMAEAMNMAVRAGRLAYLA
GRIPKKLYATASSPIEGMIG

Sequences:

>Translated_260_residues
MSNAADKLVIAGREFSSRLMVGTGKYASNEQMIKALEVSGAEIITVAVRRVNLADRGKGCLLDFIDPKKYTLLPNTAGCY
TADDAVRTCRLAREAGMSDLVKLEVLGDEKTLFPDNEELLKAAKILVKEGFTVLPYTSDDPIVCKKLEDIGCAAVMPLGA
PIGSGLGIRNPYNILIIKETVKVPVIVDAGVGTASDAAIAMELGIDGVLMNTGIAGARDPIAMAEAMNMAVRAGRLAYLA
GRIPKKLYATASSPIEGMIG
>Mature_259_residues
SNAADKLVIAGREFSSRLMVGTGKYASNEQMIKALEVSGAEIITVAVRRVNLADRGKGCLLDFIDPKKYTLLPNTAGCYT
ADDAVRTCRLAREAGMSDLVKLEVLGDEKTLFPDNEELLKAAKILVKEGFTVLPYTSDDPIVCKKLEDIGCAAVMPLGAP
IGSGLGIRNPYNILIIKETVKVPVIVDAGVGTASDAAIAMELGIDGVLMNTGIAGARDPIAMAEAMNMAVRAGRLAYLAG
RIPKKLYATASSPIEGMIG

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=47.6190476190476, Blast_Score=232, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_GEOSL (Q74FL9)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951646.1
- ProteinModelPortal:   Q74FL9
- SMR:   Q74FL9
- GeneID:   2685792
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0588
- NMPDR:   fig|243231.1.peg.584
- TIGR:   GSU0588
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- BioCyc:   GSUL243231:GSU_0588-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27432; Mature: 27301

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: ACT_SITE 102-102 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNAADKLVIAGREFSSRLMVGTGKYASNEQMIKALEVSGAEIITVAVRRVNLADRGKGC
CCCCCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEEEEHCCCCCCCCCE
LLDFIDPKKYTLLPNTAGCYTADDAVRTCRLAREAGMSDLVKLEVLGDEKTLFPDNEELL
EEEEECCCCEEECCCCCCCEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHH
KAAKILVKEGFTVLPYTSDDPIVCKKLEDIGCAAVMPLGAPIGSGLGIRNPYNILIIKET
HHHHHHHHCCCEEEEECCCCCCEEEHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEEEEC
VKVPVIVDAGVGTASDAAIAMELGIDGVLMNTGIAGARDPIAMAEAMNMAVRAGRLAYLA
CEEEEEEECCCCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHCHHHHHH
GRIPKKLYATASSPIEGMIG
HCCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SNAADKLVIAGREFSSRLMVGTGKYASNEQMIKALEVSGAEIITVAVRRVNLADRGKGC
CCCCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEEEEHCCCCCCCCCE
LLDFIDPKKYTLLPNTAGCYTADDAVRTCRLAREAGMSDLVKLEVLGDEKTLFPDNEELL
EEEEECCCCEEECCCCCCCEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHH
KAAKILVKEGFTVLPYTSDDPIVCKKLEDIGCAAVMPLGAPIGSGLGIRNPYNILIIKET
HHHHHHHHCCCEEEEECCCCCCEEEHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEEEEC
VKVPVIVDAGVGTASDAAIAMELGIDGVLMNTGIAGARDPIAMAEAMNMAVRAGRLAYLA
CEEEEEEECCCCCCCCCEEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHCHHHHHH
GRIPKKLYATASSPIEGMIG
HCCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA