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The map label for this gene is thiE

Identifier: 39995694

GI number: 39995694

Start: 620816

End: 621457

Strand: Reverse

Name: thiE

Synonym: GSU0587

Alternate gene names: 39995694

Gene position: 621457-620816 (Counterclockwise)

Preceding gene: 39995695

Following gene: 39995693

Centisome position: 16.29

GC content: 68.22

Gene sequence:

>642_bases
TTGGCTAAGGTCGATTTTTCGCTCTACCTGATCACCGACCGCCACCAGGCTGGTGGCAGGGACCTGCTCGCCGTGGTCGA
GGGAGCCCTGGCCGGTGGCGTGCGGTGCGTTCAACTCAGGGAAAAGGACCTGCCGGCCCGGACGCTGCTTGAGCTGGCCC
GGGCCATGCGCCGCCTCACCGACCGCTTCGGGGCCAGGCTCCTGATCAACGACCGGGTCGACATCGCCCTGGCCGCGGGA
GCCGACGGCGTTCACCTGGGCGAGGAGGGCATGCCGGCTGCCGTGGCGCGGGAACTGCTCGGTTCCGGGCGACTCATCGG
TGTTTCGTGTCACGGCCGCGGCGGGGCAGCAGCAGCCGTAGCACAGGGGGCAGACTTCATCACGTTCGGGCCGGTCTACC
CCACCCCGTCGAAAGCAGCCTACGGTGAGCCGGTGGGCATTGACCAACTGGCCGCAACGACGAAGGAGATACACATTCCG
GTGTTCGCCTTGGGAGGAATCAAGGAGGCGAACATCCCCGAGGCGCTGGCTGCCGGTGCGGCAGGGGTTGCCCTGATTTC
TGCCATCATCGCCGACCCGGACCCCAGGGAACGCGCCCGGGCGCTCCTTGCGCTCCTGCCGCCCCGAACCCGAGACGAGT
AA

Upstream 100 bases:

>100_bases
GCAATGAACATGGCGGTGCGGGCCGGCCGGCTCGCCTACCTGGCGGGACGCATTCCCAAGAAACTCTATGCCACGGCATC
GAGCCCCATCGAGGGGATGA

Downstream 100 bases:

>100_bases
GCCATGTCAGACCTGTTCATACATCCCGAGCTTCGCTTCCACTCCTACGGCACCTGGCTGCGCCGCCGCTTCGGCTGCAA
CGTGAGCAAGGTGAACGTGG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MAKVDFSLYLITDRHQAGGRDLLAVVEGALAGGVRCVQLREKDLPARTLLELARAMRRLTDRFGARLLINDRVDIALAAG
ADGVHLGEEGMPAAVARELLGSGRLIGVSCHGRGGAAAAVAQGADFITFGPVYPTPSKAAYGEPVGIDQLAATTKEIHIP
VFALGGIKEANIPEALAAGAAGVALISAIIADPDPRERARALLALLPPRTRDE

Sequences:

>Translated_213_residues
MAKVDFSLYLITDRHQAGGRDLLAVVEGALAGGVRCVQLREKDLPARTLLELARAMRRLTDRFGARLLINDRVDIALAAG
ADGVHLGEEGMPAAVARELLGSGRLIGVSCHGRGGAAAAVAQGADFITFGPVYPTPSKAAYGEPVGIDQLAATTKEIHIP
VFALGGIKEANIPEALAAGAAGVALISAIIADPDPRERARALLALLPPRTRDE
>Mature_212_residues
AKVDFSLYLITDRHQAGGRDLLAVVEGALAGGVRCVQLREKDLPARTLLELARAMRRLTDRFGARLLINDRVDIALAAGA
DGVHLGEEGMPAAVARELLGSGRLIGVSCHGRGGAAAAVAQGADFITFGPVYPTPSKAAYGEPVGIDQLAATTKEIHIPV
FALGGIKEANIPEALAAGAAGVALISAIIADPDPRERARALLALLPPRTRDE

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=177, Percent_Identity=35.0282485875706, Blast_Score=90, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325042, Length=224, Percent_Identity=33.4821428571429, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THE2_GEOSL (P61411)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951645.1
- ProteinModelPortal:   P61411
- GeneID:   2685827
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0587
- NMPDR:   fig|243231.1.peg.583
- TIGR:   GSU0587
- HOGENOM:   HBG754477
- OMA:   FITFGPV
- ProtClustDB:   CLSK827931
- BioCyc:   GSUL243231:GSU_0587-MONOMER
- BRENDA:   2.5.1.3
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22139; Mature: 22008

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVDFSLYLITDRHQAGGRDLLAVVEGALAGGVRCVQLREKDLPARTLLELARAMRRLT
CCEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHH
DRFGARLLINDRVDIALAAGADGVHLGEEGMPAAVARELLGSGRLIGVSCHGRGGAAAAV
HHHCCEEEEECCEEEEEECCCCCCEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHH
AQGADFITFGPVYPTPSKAAYGEPVGIDQLAATTKEIHIPVFALGGIKEANIPEALAAGA
HCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHCH
AGVALISAIIADPDPRERARALLALLPPRTRDE
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AKVDFSLYLITDRHQAGGRDLLAVVEGALAGGVRCVQLREKDLPARTLLELARAMRRLT
CEEEEEEEEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHH
DRFGARLLINDRVDIALAAGADGVHLGEEGMPAAVARELLGSGRLIGVSCHGRGGAAAAV
HHHCCEEEEECCEEEEEECCCCCCEECCCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHH
AQGADFITFGPVYPTPSKAAYGEPVGIDQLAATTKEIHIPVFALGGIKEANIPEALAAGA
HCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHCH
AGVALISAIIADPDPRERARALLALLPPRTRDE
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA