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The map label for this gene is pifA [H]

Identifier: 39995668

GI number: 39995668

Start: 592429

End: 594162

Strand: Direct

Name: pifA [H]

Synonym: GSU0561

Alternate gene names: 39995668

Gene position: 592429-594162 (Clockwise)

Preceding gene: 39995667

Following gene: 39995669

Centisome position: 15.53

GC content: 39.79

Gene sequence:

>1734_bases
TTGGAAGAAACAAAAAAGAGAGTCGTGATATTAGCCGACGATATCGATCGGTTGGAAAAAGAAGAAATTCACGCAACATT
TAGACTGGTAAAGCTGACAGCCGACTTTAAATTTACTTCATATATACTTGCATTTGATGAGCAAGTGGTTTCGTCAGCAT
TACAAGATAGATATGGCGCAGGGGCTGAGAACGCAGGAAAAGCGTTTCTTGAAAAAATTATTCAGGTGCCACTTAATTTG
CCGTCTGTCGATAGGAAGATTTTAAGAGGTTTTTGTTTTGAAGGTGTCGACAGTGCTCTCACTTTAGCTGAAATCAAGTT
AACGGAACAGCAAGTTCAAGAATTTGTAAGAGACTTTACGGCTGCTTTTGACAAAAGACTAAAAACACCAAGAAAAGCCA
GGATGTATGGCAATATTTTGATGTTCTCCTTGCCTATTCTTAAAGGAGAGACAAATCCAGTAGATGTCATGTTGATAGAA
GGTGTGAGAGTATTTTTTCCTAAAGTTTATGAGGTAATCAAAAAAAATGCGGAGATGTTCACCGGTAGCGTAAGAGATTC
GTATGGTAGTAACCGCGATAAAGAAAAATCGCATACTATAGCCACGATTGAAAAGTCAATAGACTCGTCAGACCCGGAAG
AAATTGACGGAATATTAAGCTTATTAAAATCCATGTTTCCTAAACTTCAAGCAATGTATGGAAACACACACTACGGGAGC
GATTGGGAAAAAAGCTGGTCGGAGGCTCAGAGGATATGCTCTCCTGCTTATTTTCAACGGTATTTCACATATGCGATCCC
TGAGGATGATATCCCAGACCAAACCATACAAAACATTATCGACGTTTCATCGAAAGATGATCTCGCTGATACTGCAAAAT
TATTCGATCAATTATTGACGACCCAAAATGCAGATAGGCTGATACGAAAACTACGCTCAAGGGCAAAATCTGTTTCGCAA
AAATCATCAATGACATTGGCGGGAGTTCTTTTGCTGTATTCTGACCGATTCCCTAATCCAGAAAACTTATTTAATTTCAA
TAATCCATTTGCTCAAGCCGCAATGCTTGTTAGTGATCTAATCCAGAATATCTCTGAAAAAGGTGAAAAAATATCTTTAT
CTCAGCTCTGCATCAAAAATGCACCAGACATTGAATTCTCTGCGGAAATTTTTCGATGGTTACGAGTGAAGGATAAAGAT
AGACCTGAAATAGACGGTTTTACAGAAGATGAAATAAGTGCAATAGGCAATATTTTAGCCGAGCGAATTAAATCAATTCT
GGAGGGTGATGTAGATATCACTACAGTCTACCCTAAAATATGCGGCCACTTATTGTATCAAGTAAAAAAGTACAACGGCA
AATCAGATGTCGAAGAATATCTTACCAGCTTCTTCAAAACCTGTGAACAATCTATTTTCAGGTTCTTAGACACCTTCACT
CCAACGGCCTGGGGGATGGAATCTGGAATCTCACATAAGTCAGACCTCGAAAGAGAGCAGTATAATTACATAGTTGGCTT
AGTTGACCCTGAGATCATCCTCTCAGCAATAACTCATGAAATAGGTCAACTGCCCTCCGTTTCGAAAGAATATCCAAGAG
ACGATAAAGATAGAAACCAATTGCTTGCAAACCAATTTCTTTGGTTGCACGATTATGTCCTTAAAGAGCAGGCGCGAGCT
GCCGAAGAACAAGGCCAACAAGCGGCGGCAGACGGACAAGCCGCTGCGCCTTGA

Upstream 100 bases:

>100_bases
AGCTGCCGGTGCATTTGGTCAACAGGGGATAGGTGATGGGGTAGCTCAATTCATAGCTGGTCCGTCCCTAAGCGAACTCA
GGTCTAGAATAGAAGCAGCA

Downstream 100 bases:

>100_bases
GACGTTATGGTTGCCCCAGCATTGCGGAATGAAAAAAAACAACGCACATAAAGGAAAATAGCCATGCGAAAAATCATCGC
TCTTGAATTTGTTTCCCTCG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MEETKKRVVILADDIDRLEKEEIHATFRLVKLTADFKFTSYILAFDEQVVSSALQDRYGAGAENAGKAFLEKIIQVPLNL
PSVDRKILRGFCFEGVDSALTLAEIKLTEQQVQEFVRDFTAAFDKRLKTPRKARMYGNILMFSLPILKGETNPVDVMLIE
GVRVFFPKVYEVIKKNAEMFTGSVRDSYGSNRDKEKSHTIATIEKSIDSSDPEEIDGILSLLKSMFPKLQAMYGNTHYGS
DWEKSWSEAQRICSPAYFQRYFTYAIPEDDIPDQTIQNIIDVSSKDDLADTAKLFDQLLTTQNADRLIRKLRSRAKSVSQ
KSSMTLAGVLLLYSDRFPNPENLFNFNNPFAQAAMLVSDLIQNISEKGEKISLSQLCIKNAPDIEFSAEIFRWLRVKDKD
RPEIDGFTEDEISAIGNILAERIKSILEGDVDITTVYPKICGHLLYQVKKYNGKSDVEEYLTSFFKTCEQSIFRFLDTFT
PTAWGMESGISHKSDLEREQYNYIVGLVDPEIILSAITHEIGQLPSVSKEYPRDDKDRNQLLANQFLWLHDYVLKEQARA
AEEQGQQAAADGQAAAP

Sequences:

>Translated_577_residues
MEETKKRVVILADDIDRLEKEEIHATFRLVKLTADFKFTSYILAFDEQVVSSALQDRYGAGAENAGKAFLEKIIQVPLNL
PSVDRKILRGFCFEGVDSALTLAEIKLTEQQVQEFVRDFTAAFDKRLKTPRKARMYGNILMFSLPILKGETNPVDVMLIE
GVRVFFPKVYEVIKKNAEMFTGSVRDSYGSNRDKEKSHTIATIEKSIDSSDPEEIDGILSLLKSMFPKLQAMYGNTHYGS
DWEKSWSEAQRICSPAYFQRYFTYAIPEDDIPDQTIQNIIDVSSKDDLADTAKLFDQLLTTQNADRLIRKLRSRAKSVSQ
KSSMTLAGVLLLYSDRFPNPENLFNFNNPFAQAAMLVSDLIQNISEKGEKISLSQLCIKNAPDIEFSAEIFRWLRVKDKD
RPEIDGFTEDEISAIGNILAERIKSILEGDVDITTVYPKICGHLLYQVKKYNGKSDVEEYLTSFFKTCEQSIFRFLDTFT
PTAWGMESGISHKSDLEREQYNYIVGLVDPEIILSAITHEIGQLPSVSKEYPRDDKDRNQLLANQFLWLHDYVLKEQARA
AEEQGQQAAADGQAAAP
>Mature_577_residues
MEETKKRVVILADDIDRLEKEEIHATFRLVKLTADFKFTSYILAFDEQVVSSALQDRYGAGAENAGKAFLEKIIQVPLNL
PSVDRKILRGFCFEGVDSALTLAEIKLTEQQVQEFVRDFTAAFDKRLKTPRKARMYGNILMFSLPILKGETNPVDVMLIE
GVRVFFPKVYEVIKKNAEMFTGSVRDSYGSNRDKEKSHTIATIEKSIDSSDPEEIDGILSLLKSMFPKLQAMYGNTHYGS
DWEKSWSEAQRICSPAYFQRYFTYAIPEDDIPDQTIQNIIDVSSKDDLADTAKLFDQLLTTQNADRLIRKLRSRAKSVSQ
KSSMTLAGVLLLYSDRFPNPENLFNFNNPFAQAAMLVSDLIQNISEKGEKISLSQLCIKNAPDIEFSAEIFRWLRVKDKD
RPEIDGFTEDEISAIGNILAERIKSILEGDVDITTVYPKICGHLLYQVKKYNGKSDVEEYLTSFFKTCEQSIFRFLDTFT
PTAWGMESGISHKSDLEREQYNYIVGLVDPEIILSAITHEIGQLPSVSKEYPRDDKDRNQLLANQFLWLHDYVLKEQARA
AEEQGQQAAADGQAAAP

Specific function: Responsible for the exclusion of phage T7 by plasmid F. Growth of bacteriophage T7 is inhibited in cells of E.coli that carries the plasmid F [H]

COG id: COG4928

COG function: function code R; Predicted P-loop ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 KAP NTPase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011646 [H]

Pfam domain/function: PF07693 KAP_NTPase [H]

EC number: NA

Molecular weight: Translated: 65557; Mature: 65557

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEETKKRVVILADDIDRLEKEEIHATFRLVKLTADFKFTSYILAFDEQVVSSALQDRYGA
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHCCC
GAENAGKAFLEKIIQVPLNLPSVDRKILRGFCFEGVDSALTLAEIKLTEQQVQEFVRDFT
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
AAFDKRLKTPRKARMYGNILMFSLPILKGETNPVDVMLIEGVRVFFPKVYEVIKKNAEMF
HHHHHHHCCCHHHHHHHHHHHHEEHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
TGSVRDSYGSNRDKEKSHTIATIEKSIDSSDPEEIDGILSLLKSMFPKLQAMYGNTHYGS
CCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DWEKSWSEAQRICSPAYFQRYFTYAIPEDDIPDQTIQNIIDVSSKDDLADTAKLFDQLLT
CHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TQNADRLIRKLRSRAKSVSQKSSMTLAGVLLLYSDRFPNPENLFNFNNPFAQAAMLVSDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHH
IQNISEKGEKISLSQLCIKNAPDIEFSAEIFRWLRVKDKDRPEIDGFTEDEISAIGNILA
HHHHHHCCCEEEHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
ERIKSILEGDVDITTVYPKICGHLLYQVKKYNGKSDVEEYLTSFFKTCEQSIFRFLDTFT
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PTAWGMESGISHKSDLEREQYNYIVGLVDPEIILSAITHEIGQLPSVSKEYPRDDKDRNQ
CCHHCHHHCCCCHHHCCHHHHCEEEECCCHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHH
LLANQFLWLHDYVLKEQARAAEEQGQQAAADGQAAAP
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCC
>Mature Secondary Structure
MEETKKRVVILADDIDRLEKEEIHATFRLVKLTADFKFTSYILAFDEQVVSSALQDRYGA
CCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHCCC
GAENAGKAFLEKIIQVPLNLPSVDRKILRGFCFEGVDSALTLAEIKLTEQQVQEFVRDFT
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
AAFDKRLKTPRKARMYGNILMFSLPILKGETNPVDVMLIEGVRVFFPKVYEVIKKNAEMF
HHHHHHHCCCHHHHHHHHHHHHEEHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHH
TGSVRDSYGSNRDKEKSHTIATIEKSIDSSDPEEIDGILSLLKSMFPKLQAMYGNTHYGS
CCHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DWEKSWSEAQRICSPAYFQRYFTYAIPEDDIPDQTIQNIIDVSSKDDLADTAKLFDQLLT
CHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TQNADRLIRKLRSRAKSVSQKSSMTLAGVLLLYSDRFPNPENLFNFNNPFAQAAMLVSDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHH
IQNISEKGEKISLSQLCIKNAPDIEFSAEIFRWLRVKDKDRPEIDGFTEDEISAIGNILA
HHHHHHCCCEEEHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
ERIKSILEGDVDITTVYPKICGHLLYQVKKYNGKSDVEEYLTSFFKTCEQSIFRFLDTFT
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
PTAWGMESGISHKSDLEREQYNYIVGLVDPEIILSAITHEIGQLPSVSKEYPRDDKDRNQ
CCHHCHHHCCCCHHHCCHHHHCEEEECCCHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHH
LLANQFLWLHDYVLKEQARAAEEQGQQAAADGQAAAP
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 5283385 [H]