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The map label for this gene is cls [H]

Identifier: 39995590

GI number: 39995590

Start: 512758

End: 513948

Strand: Direct

Name: cls [H]

Synonym: GSU0482

Alternate gene names: 39995590

Gene position: 512758-513948 (Clockwise)

Preceding gene: 39995589

Following gene: 39995591

Centisome position: 13.44

GC content: 62.47

Gene sequence:

>1191_bases
ATGTCCATCGTTCGCCGCAAAATGATTATGGGCACTCCCCGCGCCCCCCGCTTTCTGAGGGTCTTCAGGCGGAACGCCGA
GGCAGCCCATTCCCGGGGAAACAGGGTGAAACTGTTCACCTCGGGGGCTGATTTCTTTCCGGCCATGCTCGGCGCCTTTG
CCGAGGCGCGCCACCATATCCACGCCGAGTTCTATATTGTGCGCGACGACGCCACGGGCGCGGCCTTTGCCGAAGCACTG
CTGGCCGCCGCTGCCCGCGGGGTGGAAGTCTCCCTTATCTATGACTACATTGGTTGCTTCGACACACCTTCCGCCTATTT
CCGCCGGCTGGAACAGGGCGGAGTTCGCTGCCTCGCCTTCAATCCTCCCCCCTTCAAGGGGGGAATCGCTTGGTTCGACA
AGCGGGATCACCGCAAGATGGCTGTGGTCGACGGGGAAACCGCGTTCACGGGCGGAGTCAATGTCGGCGACGAGTATTCG
GGGTTCGGCTTTCCCCATGAACGGTGGAGGGATGTGGGCATCAGGATCGACGGACCGGCGGTCATAGATCTGTCGCGGCT
CTTTCGGGGGTTCTGGCTTGCTGAGAAGGGGAGTTCCTCACCGGCTTGGCATCAGGAGCGCCTGCCCGTGGCCGCGGTCG
GTGATGCCGACGTAATGGTCATCAGTGACGGCCCTTACCATGCCCGTTCCTTTATCCGCAATGCCTTCCGTATCGCCATG
GCCGGCGCGGGCGAGTCGATCCGGATCATGAATCCTTACTTCGTGCCGGGACCGCGGGTGGTTCGGTCTCTGCTGCGGGC
CGTCCGGCGGGGCGTCAGAGTCCAGATCATACTTCCCGCCAAGAACGATGTGCCCATCGTGCGTCTCGTGAGCCGCAGCT
ACTATACCCCCCTCCTGCGGGAGGGAATCGAACTCTTCGAGCGCCAGGGGCCGGTTCTCCACGCCAAGGTCATGCTCATT
GACGACGCCTGGGGGGTCGTCGGCTCGGCAAATCTCGACCAGCGCAGTTTTCACCGCAACTATGAGGTGAATACCATCGT
TGCCAGCCACGAGTTCGGCAGCCAGGTGGCGGAAATGTTCGCCGAGGACCTTGCCGGATCGCGACGTGTGGTCCTTGAGG
AACACGAGCAGCGGGGCTGGCATGTACGCTTGCTGGAGCGTCTCTGCGGATCGGTGAGCTGGTTTCTGTAG

Upstream 100 bases:

>100_bases
TAGGGGCGCGGGGGAAACTCCGCGCCCCTTTGTTGCGTCATGCCCATCTTCCCTTGCTCCCCCGGCGTGCCTGCGTTACC
CTTGAAGCAGGAGCAAGGCC

Downstream 100 bases:

>100_bases
TGTCTTCACCCTTGTCCCTCATCCATTCCTGTTGTACGATGACTCTGGGCTTCCATGCCCATGAAACGCCCTGTCCGGCG
AGGTATTCCCATGACTAGAA

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSIVRRKMIMGTPRAPRFLRVFRRNAEAAHSRGNRVKLFTSGADFFPAMLGAFAEARHHIHAEFYIVRDDATGAAFAEAL
LAAAARGVEVSLIYDYIGCFDTPSAYFRRLEQGGVRCLAFNPPPFKGGIAWFDKRDHRKMAVVDGETAFTGGVNVGDEYS
GFGFPHERWRDVGIRIDGPAVIDLSRLFRGFWLAEKGSSSPAWHQERLPVAAVGDADVMVISDGPYHARSFIRNAFRIAM
AGAGESIRIMNPYFVPGPRVVRSLLRAVRRGVRVQIILPAKNDVPIVRLVSRSYYTPLLREGIELFERQGPVLHAKVMLI
DDAWGVVGSANLDQRSFHRNYEVNTIVASHEFGSQVAEMFAEDLAGSRRVVLEEHEQRGWHVRLLERLCGSVSWFL

Sequences:

>Translated_396_residues
MSIVRRKMIMGTPRAPRFLRVFRRNAEAAHSRGNRVKLFTSGADFFPAMLGAFAEARHHIHAEFYIVRDDATGAAFAEAL
LAAAARGVEVSLIYDYIGCFDTPSAYFRRLEQGGVRCLAFNPPPFKGGIAWFDKRDHRKMAVVDGETAFTGGVNVGDEYS
GFGFPHERWRDVGIRIDGPAVIDLSRLFRGFWLAEKGSSSPAWHQERLPVAAVGDADVMVISDGPYHARSFIRNAFRIAM
AGAGESIRIMNPYFVPGPRVVRSLLRAVRRGVRVQIILPAKNDVPIVRLVSRSYYTPLLREGIELFERQGPVLHAKVMLI
DDAWGVVGSANLDQRSFHRNYEVNTIVASHEFGSQVAEMFAEDLAGSRRVVLEEHEQRGWHVRLLERLCGSVSWFL
>Mature_395_residues
SIVRRKMIMGTPRAPRFLRVFRRNAEAAHSRGNRVKLFTSGADFFPAMLGAFAEARHHIHAEFYIVRDDATGAAFAEALL
AAAARGVEVSLIYDYIGCFDTPSAYFRRLEQGGVRCLAFNPPPFKGGIAWFDKRDHRKMAVVDGETAFTGGVNVGDEYSG
FGFPHERWRDVGIRIDGPAVIDLSRLFRGFWLAEKGSSSPAWHQERLPVAAVGDADVMVISDGPYHARSFIRNAFRIAMA
GAGESIRIMNPYFVPGPRVVRSLLRAVRRGVRVQIILPAKNDVPIVRLVSRSYYTPLLREGIELFERQGPVLHAKVMLID
DAWGVVGSANLDQRSFHRNYEVNTIVASHEFGSQVAEMFAEDLAGSRRVVLEEHEQRGWHVRLLERLCGSVSWFL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=387, Percent_Identity=29.7157622739018, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787007, Length=327, Percent_Identity=32.7217125382263, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87081826, Length=393, Percent_Identity=27.735368956743, Blast_Score=95, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 44385; Mature: 44254

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVRRKMIMGTPRAPRFLRVFRRNAEAAHSRGNRVKLFTSGADFFPAMLGAFAEARHHI
CCHHHHHHHHCCCCCHHHHHHHHHCCHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCE
HAEFYIVRDDATGAAFAEALLAAAARGVEVSLIYDYIGCFDTPSAYFRRLEQGGVRCLAF
EEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCHHHHHHHHHHCCCEEEEE
NPPPFKGGIAWFDKRDHRKMAVVDGETAFTGGVNVGDEYSGFGFPHERWRDVGIRIDGPA
CCCCCCCCEEECCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCHHHHHCCCEEECCCC
VIDLSRLFRGFWLAEKGSSSPAWHQERLPVAAVGDADVMVISDGPYHARSFIRNAFRIAM
EEEHHHHHHHEEEECCCCCCCCCHHHCCCEEEECCCCEEEECCCCHHHHHHHHHHHHEEE
AGAGESIRIMNPYFVPGPRVVRSLLRAVRRGVRVQIILPAKNDVPIVRLVSRSYYTPLLR
ECCCCEEEEECCEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHEEHHHHHCCHHHH
EGIELFERQGPVLHAKVMLIDDAWGVVGSANLDQRSFHRNYEVNTIVASHEFGSQVAEMF
HHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCHHHHHCCCEEEEEEECCHHHHHHHHHH
AEDLAGSRRVVLEEHEQRGWHVRLLERLCGSVSWFL
HHHHCCCCEEEEEHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIVRRKMIMGTPRAPRFLRVFRRNAEAAHSRGNRVKLFTSGADFFPAMLGAFAEARHHI
CHHHHHHHHCCCCCHHHHHHHHHCCHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHCE
HAEFYIVRDDATGAAFAEALLAAAARGVEVSLIYDYIGCFDTPSAYFRRLEQGGVRCLAF
EEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCHHHHHHHHHHCCCEEEEE
NPPPFKGGIAWFDKRDHRKMAVVDGETAFTGGVNVGDEYSGFGFPHERWRDVGIRIDGPA
CCCCCCCCEEECCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCHHHHHCCCEEECCCC
VIDLSRLFRGFWLAEKGSSSPAWHQERLPVAAVGDADVMVISDGPYHARSFIRNAFRIAM
EEEHHHHHHHEEEECCCCCCCCCHHHCCCEEEECCCCEEEECCCCHHHHHHHHHHHHEEE
AGAGESIRIMNPYFVPGPRVVRSLLRAVRRGVRVQIILPAKNDVPIVRLVSRSYYTPLLR
ECCCCEEEEECCEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHEEHHHHHCCHHHH
EGIELFERQGPVLHAKVMLIDDAWGVVGSANLDQRSFHRNYEVNTIVASHEFGSQVAEMF
HHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCHHHHHCCCEEEEEEECCHHHHHHHHHH
AEDLAGSRRVVLEEHEQRGWHVRLLERLCGSVSWFL
HHHHCCCCEEEEEHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]