Click here to switch to the map view.

The map label for this gene is mdtE [H]

Identifier: 39995500

GI number: 39995500

Start: 423819

End: 424931

Strand: Direct

Name: mdtE [H]

Synonym: GSU0392

Alternate gene names: 39995500

Gene position: 423819-424931 (Clockwise)

Preceding gene: 39995499

Following gene: 39995501

Centisome position: 11.11

GC content: 67.21

Gene sequence:

>1113_bases
ATGAGGATGGGGAGAGGAGTGAGAGCGGCCTGTGGCGTCGTCCTCCTTGCTGCGGCCCTCACGGGCTGTGGCCATAAGGC
GGATAAGGCGGAATCGAAGCAGCCCCCCGTCGTGACGGGAGTGACACTGATCCCGGCGGCCGGAGAGGCTATCCCGGACG
GCGCAGAGGTGGTGGGGACGGTACGCGCCGCAGACGCCGCCGTCATTGCAGCCCGGCTTCCCGCCACCGTAACTGGCGTT
CTCGTGCGGGAGGGGGACCGTGTGGGCAGGGGAAAGCTCCTGGTGACCCTTGAGGCCGCCGAGAGTTCGGCCCAGGCGTC
CGCTGCGGCCGCCCAGGTGGCCGAGGCGGAGCGGGCGCTGGACGAAGCCCGTGCCCGCAAACGGCTGGCCGACGCAACCT
ATGAGCGCTACGCCAACCTCTTCAAGGATGAGGCAGTGACCCGTCAAGAGATGGACAACCGTCGCGCCGACCGGGACGTG
GCCGAGCAGGGTGTCGAGCGGGCAACTGCCCGTTTGATGGCGAGCAGGGAGAGTGCGCGGGCGGCCGGGGTCGTGGCGGG
TTACACCCGCATCGTCTCGCCGCTTGCCGGGGTAGTGACCGCAAAGCAGGTGGAAAACGGTATGACCGTGTTTCCGGGTA
CGCCGCTGATGACTATCGAGGGTGACGGGGGGCGCCGACTTGAGGCGTCCGTTCCCGAATCCCTCGCCGGTCGGGTAAGA
CTTGGCCAGCGCGTGCCGGTGACCATTGACGGGGCCGGTCTCTCGCTGGAGGGCCGGGTGGTTGAGATCGTGCCCGCCGC
CGACCCGGCGAGCAGAACCTTCACCGTCAAGGTTGATCTTGCCGGTGAAGGGATCCGGTCGGGCATGTTCGGCCGGGCCT
TTATCGCTACCGGTCAACGGCAGGGGATTCTCGTGCCGAAGTCGGCGGTGATGGAACGGGGGGCTCTCTCCTCGGTCTGG
ACGCTGGACCAGGGGAATGTCGCCCGGCTGCGACTCGTGAAAACGGGAAAACCCTTGGGCGACCGGATCGAGATCCTTTC
GGGCCTCACGGCCGGTGAGCGGGTCATCTCAGCCGGGCTGGAAAAGGTTGTTGAGGGGGCGAAAATCCAGTAA

Upstream 100 bases:

>100_bases
ACAGGAGCAGGACCGGCCTCGTGGAGCGGGAGAGCGAGTATCTGTTGGCCACGGCCCGCGTGTACCACGCGGCCGGACTA
TTTCTGAAGGAGACCGTGAA

Downstream 100 bases:

>100_bases
CGGGGATGCCCGCGGAAGAGGAGGCGCGGCAGCCCGTTGCTCCGCGCTCCGGCGCGGGGAGGCGCCGCCGCCATGCACGA
CGACGGACGCGACCCTGAAA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MRMGRGVRAACGVVLLAAALTGCGHKADKAESKQPPVVTGVTLIPAAGEAIPDGAEVVGTVRAADAAVIAARLPATVTGV
LVREGDRVGRGKLLVTLEAAESSAQASAAAAQVAEAERALDEARARKRLADATYERYANLFKDEAVTRQEMDNRRADRDV
AEQGVERATARLMASRESARAAGVVAGYTRIVSPLAGVVTAKQVENGMTVFPGTPLMTIEGDGGRRLEASVPESLAGRVR
LGQRVPVTIDGAGLSLEGRVVEIVPAADPASRTFTVKVDLAGEGIRSGMFGRAFIATGQRQGILVPKSAVMERGALSSVW
TLDQGNVARLRLVKTGKPLGDRIEILSGLTAGERVISAGLEKVVEGAKIQ

Sequences:

>Translated_370_residues
MRMGRGVRAACGVVLLAAALTGCGHKADKAESKQPPVVTGVTLIPAAGEAIPDGAEVVGTVRAADAAVIAARLPATVTGV
LVREGDRVGRGKLLVTLEAAESSAQASAAAAQVAEAERALDEARARKRLADATYERYANLFKDEAVTRQEMDNRRADRDV
AEQGVERATARLMASRESARAAGVVAGYTRIVSPLAGVVTAKQVENGMTVFPGTPLMTIEGDGGRRLEASVPESLAGRVR
LGQRVPVTIDGAGLSLEGRVVEIVPAADPASRTFTVKVDLAGEGIRSGMFGRAFIATGQRQGILVPKSAVMERGALSSVW
TLDQGNVARLRLVKTGKPLGDRIEILSGLTAGERVISAGLEKVVEGAKIQ
>Mature_370_residues
MRMGRGVRAACGVVLLAAALTGCGHKADKAESKQPPVVTGVTLIPAAGEAIPDGAEVVGTVRAADAAVIAARLPATVTGV
LVREGDRVGRGKLLVTLEAAESSAQASAAAAQVAEAERALDEARARKRLADATYERYANLFKDEAVTRQEMDNRRADRDV
AEQGVERATARLMASRESARAAGVVAGYTRIVSPLAGVVTAKQVENGMTVFPGTPLMTIEGDGGRRLEASVPESLAGRVR
LGQRVPVTIDGAGLSLEGRVVEIVPAADPASRTFTVKVDLAGEGIRSGMFGRAFIATGQRQGILVPKSAVMERGALSSVW
TLDQGNVARLRLVKTGKPLGDRIEILSGLTAGERVISAGLEKVVEGAKIQ

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=389, Percent_Identity=25.4498714652956, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87082036, Length=340, Percent_Identity=25.2941176470588, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081796, Length=331, Percent_Identity=23.5649546827795, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 38443; Mature: 38443

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMGRGVRAACGVVLLAAALTGCGHKADKAESKQPPVVTGVTLIPAAGEAIPDGAEVVGT
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHH
VRAADAAVIAARLPATVTGVLVREGDRVGRGKLLVTLEAAESSAQASAAAAQVAEAERAL
HHHHHHHHHHHHCCCHHEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
DEARARKRLADATYERYANLFKDEAVTRQEMDNRRADRDVAEQGVERATARLMASRESAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGVVAGYTRIVSPLAGVVTAKQVENGMTVFPGTPLMTIEGDGGRRLEASVPESLAGRVR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCEEECCCCHHHHHHHH
LGQRVPVTIDGAGLSLEGRVVEIVPAADPASRTFTVKVDLAGEGIRSGMFGRAFIATGQR
CCCCCCEEECCCCEEECCEEEEEEECCCCCCCEEEEEEEECCCCHHCCCCCCCEEECCCC
QGILVPKSAVMERGALSSVWTLDQGNVARLRLVKTGKPLGDRIEILSGLTAGERVISAGL
CCEECCHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHH
EKVVEGAKIQ
HHHHCCCCCC
>Mature Secondary Structure
MRMGRGVRAACGVVLLAAALTGCGHKADKAESKQPPVVTGVTLIPAAGEAIPDGAEVVGT
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHH
VRAADAAVIAARLPATVTGVLVREGDRVGRGKLLVTLEAAESSAQASAAAAQVAEAERAL
HHHHHHHHHHHHCCCHHEEEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
DEARARKRLADATYERYANLFKDEAVTRQEMDNRRADRDVAEQGVERATARLMASRESAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGVVAGYTRIVSPLAGVVTAKQVENGMTVFPGTPLMTIEGDGGRRLEASVPESLAGRVR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCEEECCCCHHHHHHHH
LGQRVPVTIDGAGLSLEGRVVEIVPAADPASRTFTVKVDLAGEGIRSGMFGRAFIATGQR
CCCCCCEEECCCCEEECCEEEEEEECCCCCCCEEEEEEEECCCCHHCCCCCCCEEECCCC
QGILVPKSAVMERGALSSVWTLDQGNVARLRLVKTGKPLGDRIEILSGLTAGERVISAGL
CCEECCHHHHHHCCCCCCEEEECCCCEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHH
EKVVEGAKIQ
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]