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The map label for this gene is oprF [H]

Identifier: 39995468

GI number: 39995468

Start: 391444

End: 392733

Strand: Direct

Name: oprF [H]

Synonym: GSU0360

Alternate gene names: 39995468

Gene position: 391444-392733 (Clockwise)

Preceding gene: 39995464

Following gene: 39995470

Centisome position: 10.26

GC content: 60.62

Gene sequence:

>1290_bases
ATGAAGCAACGAATCGTGCCCGGACTGCTTGCCTTGGCCGCGATAGCCGTCACGGCCGCGCCAACACTCGCCGAAAACAG
GAGCGGAGCAGGGTACATCTCTCCCTTTGTGGGCGGCTACACGTTTGACGGCGCACAACACCTGAAAACCCGTCCGACTT
TCGGCCTGAGAGGCGGCTACAACCTGGACAAGAACTGGGCCGCTGAACTGCTCTTCGGGTATGTCCCCACCAAGCAGAGC
AGGGGGCAGGGAAATGACGCGGATGCTTACCGTTACGGTCTCGACGTCCTCTATAACTTCATGCCCGACAGCGCTCTGGT
GCCGTTCCTCGCCGTAGGCGGCGGCGCATCCACCATCGATCACTCCGAAGGGGTGAGCGACGAGCACGAGCAGTATGTGA
ACTACGGTGGCGGCCTCAAATACTTCCTGACCGATAATTTCGCACTTCGTGGCGACGTGCGGCACATCTACATGCTCGGC
CCCGAAAACCACAATTTTGAGTACACGGTGGGACTCAACTACCTCTTTGGCGGTGAGCGCCCCGCGCCGGCCAAGGTTGC
TGCACCTCCCGAGCCGCCTAAGGCCGTCGAGCCCCCGCCTCCGGCCCCCGCGCCCAAGAAAGAGATTGCACCGCCTCCTC
CGCCTCCGGCTCCTGCACCCAAGGATACGGACGGTGACGGGGTAATCGATGACCTGGACAAGTGCCCCGATACCCCTGCG
GGCGTGAAGGTCGATAGTGTCGGTTGTCCTCTCGATACGGACAAGGACGGCGTTTACGATTACCTGGACAAGTGCCCCGG
CACCCCTGCCGGCGTGAAGGTGGACAGCGTTGGTTGTCCTCTCGATACTGACGGCGACGGCGTCTATGACTATCTGGACA
AGTGCCCCGACACGCCCAAGGATCTGGCGGTCGACGCAAAAGGTTGCCCCGAGCGCATCTGCATCACTCTCGGCGTTCAG
TTTGACACCGACAAGGCCGTGGTCAAGCCTCAGTACAATGATGAGATCAAGAAGGCCGCCGACTTCCTGAAAGAGTACCC
CGACGCGACCGCCGTCATTGAAGGGCACACCGACAGCGTCGGAAGCGATGCTTACAATCAGAAGCTTTCGGAGCGGCGTG
CCAAGGCGGTCATGAAGTATCTGGTCGACAAGTTCGGTATCAGCGCCAAGCGTCTCTCGGCCAAGGGCTTCGGCGAGTCC
AAGCCGATTGCCGACAACGCTACCGAAGAAGGTCGGTACAAGAACCGCCGGGTTGTGGCGGTCATCGACTGCGGTATGAA
GAAACGCTGA

Upstream 100 bases:

>100_bases
TCACCATGTCATGAGCAGCGACTGTACGGTTCAGGGCTGTGTCGTTCCGACGCGGGCAGTGAGCCGTCACGCAGCATCTA
CTACTCAAAGGAGGTCATCA

Downstream 100 bases:

>100_bases
TCTGCTGCTTCCCGGAAAGGAAAAACGGCGCGTTGCTCAGGCAACGCGCCGTGTGTATTGGTTGCTGGCGAGGCCCAATT
CGATGCCTTGCCCATTCGCG

Product: OmpA domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKQRIVPGLLALAAIAVTAAPTLAENRSGAGYISPFVGGYTFDGAQHLKTRPTFGLRGGYNLDKNWAAELLFGYVPTKQS
RGQGNDADAYRYGLDVLYNFMPDSALVPFLAVGGGASTIDHSEGVSDEHEQYVNYGGGLKYFLTDNFALRGDVRHIYMLG
PENHNFEYTVGLNYLFGGERPAPAKVAAPPEPPKAVEPPPPAPAPKKEIAPPPPPPAPAPKDTDGDGVIDDLDKCPDTPA
GVKVDSVGCPLDTDKDGVYDYLDKCPGTPAGVKVDSVGCPLDTDGDGVYDYLDKCPDTPKDLAVDAKGCPERICITLGVQ
FDTDKAVVKPQYNDEIKKAADFLKEYPDATAVIEGHTDSVGSDAYNQKLSERRAKAVMKYLVDKFGISAKRLSAKGFGES
KPIADNATEEGRYKNRRVVAVIDCGMKKR

Sequences:

>Translated_429_residues
MKQRIVPGLLALAAIAVTAAPTLAENRSGAGYISPFVGGYTFDGAQHLKTRPTFGLRGGYNLDKNWAAELLFGYVPTKQS
RGQGNDADAYRYGLDVLYNFMPDSALVPFLAVGGGASTIDHSEGVSDEHEQYVNYGGGLKYFLTDNFALRGDVRHIYMLG
PENHNFEYTVGLNYLFGGERPAPAKVAAPPEPPKAVEPPPPAPAPKKEIAPPPPPPAPAPKDTDGDGVIDDLDKCPDTPA
GVKVDSVGCPLDTDKDGVYDYLDKCPGTPAGVKVDSVGCPLDTDGDGVYDYLDKCPDTPKDLAVDAKGCPERICITLGVQ
FDTDKAVVKPQYNDEIKKAADFLKEYPDATAVIEGHTDSVGSDAYNQKLSERRAKAVMKYLVDKFGISAKRLSAKGFGES
KPIADNATEEGRYKNRRVVAVIDCGMKKR
>Mature_429_residues
MKQRIVPGLLALAAIAVTAAPTLAENRSGAGYISPFVGGYTFDGAQHLKTRPTFGLRGGYNLDKNWAAELLFGYVPTKQS
RGQGNDADAYRYGLDVLYNFMPDSALVPFLAVGGGASTIDHSEGVSDEHEQYVNYGGGLKYFLTDNFALRGDVRHIYMLG
PENHNFEYTVGLNYLFGGERPAPAKVAAPPEPPKAVEPPPPAPAPKKEIAPPPPPPAPAPKDTDGDGVIDDLDKCPDTPA
GVKVDSVGCPLDTDKDGVYDYLDKCPGTPAGVKVDSVGCPLDTDGDGVYDYLDKCPDTPKDLAVDAKGCPERICITLGVQ
FDTDKAVVKPQYNDEIKKAADFLKEYPDATAVIEGHTDSVGSDAYNQKLSERRAKAVMKYLVDKFGISAKRLSAKGFGES
KPIADNATEEGRYKNRRVVAVIDCGMKKR

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=134, Percent_Identity=34.3283582089552, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787191, Length=108, Percent_Identity=40.7407407407407, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786962, Length=103, Percent_Identity=38.8349514563107, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 46054; Mature: 46054

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS01068 OMPA_1 ; PS51123 OMPA_2 ; PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQRIVPGLLALAAIAVTAAPTLAENRSGAGYISPFVGGYTFDGAQHLKTRPTFGLRGGY
CCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCEEECCHHHHCCCCCCCCCCCC
NLDKNWAAELLFGYVPTKQSRGQGNDADAYRYGLDVLYNFMPDSALVPFLAVGGGASTID
CCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
HSEGVSDEHEQYVNYGGGLKYFLTDNFALRGDVRHIYMLGPENHNFEYTVGLNYLFGGER
CCCCCCHHHHHHHHCCCCEEEEEECCEEEECCCEEEEEECCCCCCEEEEEEEEEEECCCC
PAPAKVAAPPEPPKAVEPPPPAPAPKKEIAPPPPPPAPAPKDTDGDGVIDDLDKCPDTPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCC
GVKVDSVGCPLDTDKDGVYDYLDKCPGTPAGVKVDSVGCPLDTDGDGVYDYLDKCPDTPK
CEEECCCCCCCCCCCCHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCHH
DLAVDAKGCPERICITLGVQFDTDKAVVKPQYNDEIKKAADFLKEYPDATAVIEGHTDSV
HEEECCCCCCCEEEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCC
GSDAYNQKLSERRAKAVMKYLVDKFGISAKRLSAKGFGESKPIADNATEEGRYKNRRVVA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEE
VIDCGMKKR
EECCCCCCC
>Mature Secondary Structure
MKQRIVPGLLALAAIAVTAAPTLAENRSGAGYISPFVGGYTFDGAQHLKTRPTFGLRGGY
CCCCHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCEEECCHHHHCCCCCCCCCCCC
NLDKNWAAELLFGYVPTKQSRGQGNDADAYRYGLDVLYNFMPDSALVPFLAVGGGASTID
CCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCC
HSEGVSDEHEQYVNYGGGLKYFLTDNFALRGDVRHIYMLGPENHNFEYTVGLNYLFGGER
CCCCCCHHHHHHHHCCCCEEEEEECCEEEECCCEEEEEECCCCCCEEEEEEEEEEECCCC
PAPAKVAAPPEPPKAVEPPPPAPAPKKEIAPPPPPPAPAPKDTDGDGVIDDLDKCPDTPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCC
GVKVDSVGCPLDTDKDGVYDYLDKCPGTPAGVKVDSVGCPLDTDGDGVYDYLDKCPDTPK
CEEECCCCCCCCCCCCHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCCCHH
DLAVDAKGCPERICITLGVQFDTDKAVVKPQYNDEIKKAADFLKEYPDATAVIEGHTDSV
HEEECCCCCCCEEEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCCC
GSDAYNQKLSERRAKAVMKYLVDKFGISAKRLSAKGFGESKPIADNATEEGRYKNRRVVA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEE
VIDCGMKKR
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]