Click here to switch to the map view.

The map label for this gene is nuoI-1

Identifier: 39995455

GI number: 39995455

Start: 376470

End: 376868

Strand: Direct

Name: nuoI-1

Synonym: GSU0346

Alternate gene names: 39995455

Gene position: 376470-376868 (Clockwise)

Preceding gene: 39995454

Following gene: 39995456

Centisome position: 9.87

GC content: 47.12

Gene sequence:

>399_bases
ATGATAATGCCGTTAATTAATGGACTCAAAATCACGTTAAAGCATATGTTCATGAAGCCGGTAACGCTCCAGTACCCTGA
TGAGCGACCGACTCCATCCCCTAACTTCCGCGGTCTTCATGCGCTGAAGGTATCGCACGATAAAGCAAAGTGCGTAGCGT
GTTACCTTTGCCCGACAGTCTGCCCGGCGAAGTGCATAACGGTTGAGGCTGGCGAAGACGCAACTCATGACAAGTATGCA
GAACGCTATGAGATCGACATGCTGAGGTGCATCTTCTGTGGTTACTGCGTTGAAGCCTGTCCTGTAGATGCTCTGAAGAT
GACTGGCCAGTTTGAACTTGCAAACTATAAGCGTGAAGACTTCATCTTTGTCAAAGAGAGACTCTTAGAAAAGAAATAA

Upstream 100 bases:

>100_bases
TTTGGGTTGGAAGGTCTTCCTCCCGCTTACCTTGGTGAATATTATTGTCACGGGTATAGTAGTGTCGCTCCAATCGTAAC
TCCACTACGAGGTCACAGCC

Downstream 100 bases:

>100_bases
AGGAGCAGCAATGGAAACGTTCTTCTTCACGATCGTGGCAGCGGTTGCTGTACTGGCCAGCATACTGGTCATCACCTGCA
AGAACCCGATCAACAGCGCG

Product: NADH dehydrogenase I subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I 1; NDH-1 subunit I 1

Number of amino acids: Translated: 132; Mature: 132

Protein sequence:

>132_residues
MIMPLINGLKITLKHMFMKPVTLQYPDERPTPSPNFRGLHALKVSHDKAKCVACYLCPTVCPAKCITVEAGEDATHDKYA
ERYEIDMLRCIFCGYCVEACPVDALKMTGQFELANYKREDFIFVKERLLEKK

Sequences:

>Translated_132_residues
MIMPLINGLKITLKHMFMKPVTLQYPDERPTPSPNFRGLHALKVSHDKAKCVACYLCPTVCPAKCITVEAGEDATHDKYA
ERYEIDMLRCIFCGYCVEACPVDALKMTGQFELANYKREDFIFVKERLLEKK
>Mature_132_residues
MIMPLINGLKITLKHMFMKPVTLQYPDERPTPSPNFRGLHALKVSHDKAKCVACYLCPTVCPAKCITVEAGEDATHDKYA
ERYEIDMLRCIFCGYCVEACPVDALKMTGQFELANYKREDFIFVKERLLEKK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=128, Percent_Identity=43.75, Blast_Score=120, Evalue=4e-28,
Organism=Escherichia coli, GI1788617, Length=116, Percent_Identity=42.2413793103448, Blast_Score=98, Evalue=2e-22,
Organism=Escherichia coli, GI1789075, Length=102, Percent_Identity=37.2549019607843, Blast_Score=71, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17555194, Length=126, Percent_Identity=42.8571428571429, Blast_Score=114, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17864306, Length=126, Percent_Identity=45.2380952380952, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI1_GEOSL (Q74GA0)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951406.1
- ProteinModelPortal:   Q74GA0
- SMR:   Q74GA0
- GeneID:   2685556
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0346
- NMPDR:   fig|243231.1.peg.344
- TIGR:   GSU0346
- HOGENOM:   HBG724359
- OMA:   HALKVSH
- ProtClustDB:   CLSK827769
- BioCyc:   GSUL243231:GSU_0346-MONOMER
- BRENDA:   1.6.99.5
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 15138; Mature: 15138

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
11.4 %Cys+Met (Translated Protein)
6.8 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
11.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMPLINGLKITLKHMFMKPVTLQYPDERPTPSPNFRGLHALKVSHDKAKCVACYLCPTV
CCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEECCCCHHEEEEEECCCC
CPAKCITVEAGEDATHDKYAERYEIDMLRCIFCGYCVEACPVDALKMTGQFELANYKRED
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEECCCCCCH
FIFVKERLLEKK
HHHHHHHHHCCC
>Mature Secondary Structure
MIMPLINGLKITLKHMFMKPVTLQYPDERPTPSPNFRGLHALKVSHDKAKCVACYLCPTV
CCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEECCCCHHEEEEEECCCC
CPAKCITVEAGEDATHDKYAERYEIDMLRCIFCGYCVEACPVDALKMTGQFELANYKRED
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEECCCCCCH
FIFVKERLLEKK
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA