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The map label for this gene is nouF [H]

Identifier: 39995452

GI number: 39995452

Start: 371053

End: 372828

Strand: Direct

Name: nouF [H]

Synonym: GSU0343

Alternate gene names: 39995452

Gene position: 371053-372828 (Clockwise)

Preceding gene: 39995451

Following gene: 39995453

Centisome position: 9.73

GC content: 54.0

Gene sequence:

>1776_bases
ATGGGCGAAGCGATAAAGATTCTGATCTGTCAGGGGACCGGTGGTGTCTCAGCGGGTGCTAAAAAGGTTGAGGCGGAGTT
CCTCCGTGTTATCGGTGAAAAGGGCGTCGATGCCACCGTCGGCAAACGATGTGACGTCATCAAGACCGGCTGTCGCGGCC
TGTGTGCAAACGATGTTCTTGTGGATATTGTAGATCCCGAGCTTGGGCGTGTAACTTACGATTTCGTTCTGCCAGAAGAA
GTCGAAAAGATTGTGGCCGAGCATATCGTCAACCGGACCCCCCTTGAGAAGCGCAAAGCAAAACCGTACTACAACACGTT
CGTAGACCAGCAGATGAGGGTTGTTATGACCGGGTGCGGTCAGATCGACCCGGAAAGTCTCGATGCGTATCTAGAAGAGG
ACGGCTTCAAGGCAATTGAAAAATGCGTTAAGGAAATGACTCCCGCTGCAGTTATCGACGAAGTCAAGAAGTCTGGACTT
CGAGGTAGAGGGGGCGGTGGCTTCCCGACGGGCATGAAGTGGTCTTTCTGTGCTGCGACGCCCGGCAAACTGAAGTATGT
CATCTGCAATGCGGACGAAGGTGACCCCGGTGCTTTCATGGACCGGTCGATTCTCGAGGGTGACCCTTACTGCATCATTG
AAGGAATGATGATAGCGGCATACGCAATCGGCTGTACTGCTGGCTATGTGTACGTACGGGCAGAGTACCCACTTGCTATT
GAGCGTCTGCAGAAGGCTCTCGATGTTTGCTACGAAAAGGGCTACCTCGGCAAGAACGTCATGGGCTGGGGATTCGACTT
CGACCTTCGGATCAAGAAGGGTGCGGGGGCCTTTGTGTGCGGTGAGGAAACCGCGCTCATGGCTTCGATCGAAGGCGAGC
GGGGAATGCCTCGCCCTCGTCCGCCGTTCCCCGCTGTCAAAGGTCTGTGGGGGAAGCCTACCAACATTAACAACGTTGAG
ACGTTCGCCAACGTGCGTCACATCATCCTGAAGGGTGCTGACTGGTACGCATCTCTCGGTACTGATACCACAAAAGGGAC
AAAGATCTTCGCGGTCACCGGTAAAGTAAAGCACACGGGACTTGTGGAAGTCCCGGCGGGTATGAGCGTGCGCGAAGTTA
TCTATAACGTTTGCGGCGGCATCGCCAACAACCGGAAGTTCAAAGCAGTTCAGGCAGGTGGACCATCAGGCGGATGTATT
CCCTCAGAAGTTCTCGATACACCGGTTGATTATGACTCGCTTATCAAGGCCGGTGCCATGATGGGCTCCGGTGGTCTCGT
CGTCATGGACGAAACTACCTGCATGGTCGACGTAGCACGCTTCTTCCTTACCTTCACGCGGATGGAATCATGCGGTAAAT
GCGTTCCGTGCCGCATTGGACTCAAGGCGATGTTGGACATCCTCGAACGCATCACCGAAGGAAGAGGCCAAGCTGGTGAC
ATTGAGACACTGCTTGAGATGGGGAGCACCATCAAAAAGGCCTCACTCTGCGGTCTGGGCCAGACAGCACCCAACCCGAT
CCTCTCCACGATCAAGTATTTCCGTGAGGAGTATGAAGCGCATATCAATGATCGGCGTTGCCCGTCGAACTGCTGTAAAG
AGCTTCTGTTGTGGCAAGTAGTCGAGGAAAAGTGCGTTAAGTGCGGAGCCTGCTTCAAGGCATGCCCTGTTGACGCAATT
ATCTGGGAGAAGGGGCAAATTGCAATCCTAGATAAAGAGAAATGCACCAAATGCAAATCCTGCTACGACGCCTGCCGGTT
CATGGCCATCGAGTAG

Upstream 100 bases:

>100_bases
GCAAGATGACGGTACAAAAGGTCGAAGAAATTATTGCCGAATACAATCAGAGACCGATGAAGTAACAGACCCAACCATTC
ACATCAGAGGATAGAGCGTT

Downstream 100 bases:

>100_bases
CGAGCTGTAAGTCATACATCCGAGGGAGACAGAGGTCGAGATGGTAACTCTTACAATCGATGACAAGCAGGTAACGGTAC
CGAAAGACGCTACCATATTT

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MGEAIKILICQGTGGVSAGAKKVEAEFLRVIGEKGVDATVGKRCDVIKTGCRGLCANDVLVDIVDPELGRVTYDFVLPEE
VEKIVAEHIVNRTPLEKRKAKPYYNTFVDQQMRVVMTGCGQIDPESLDAYLEEDGFKAIEKCVKEMTPAAVIDEVKKSGL
RGRGGGGFPTGMKWSFCAATPGKLKYVICNADEGDPGAFMDRSILEGDPYCIIEGMMIAAYAIGCTAGYVYVRAEYPLAI
ERLQKALDVCYEKGYLGKNVMGWGFDFDLRIKKGAGAFVCGEETALMASIEGERGMPRPRPPFPAVKGLWGKPTNINNVE
TFANVRHIILKGADWYASLGTDTTKGTKIFAVTGKVKHTGLVEVPAGMSVREVIYNVCGGIANNRKFKAVQAGGPSGGCI
PSEVLDTPVDYDSLIKAGAMMGSGGLVVMDETTCMVDVARFFLTFTRMESCGKCVPCRIGLKAMLDILERITEGRGQAGD
IETLLEMGSTIKKASLCGLGQTAPNPILSTIKYFREEYEAHINDRRCPSNCCKELLLWQVVEEKCVKCGACFKACPVDAI
IWEKGQIAILDKEKCTKCKSCYDACRFMAIE

Sequences:

>Translated_591_residues
MGEAIKILICQGTGGVSAGAKKVEAEFLRVIGEKGVDATVGKRCDVIKTGCRGLCANDVLVDIVDPELGRVTYDFVLPEE
VEKIVAEHIVNRTPLEKRKAKPYYNTFVDQQMRVVMTGCGQIDPESLDAYLEEDGFKAIEKCVKEMTPAAVIDEVKKSGL
RGRGGGGFPTGMKWSFCAATPGKLKYVICNADEGDPGAFMDRSILEGDPYCIIEGMMIAAYAIGCTAGYVYVRAEYPLAI
ERLQKALDVCYEKGYLGKNVMGWGFDFDLRIKKGAGAFVCGEETALMASIEGERGMPRPRPPFPAVKGLWGKPTNINNVE
TFANVRHIILKGADWYASLGTDTTKGTKIFAVTGKVKHTGLVEVPAGMSVREVIYNVCGGIANNRKFKAVQAGGPSGGCI
PSEVLDTPVDYDSLIKAGAMMGSGGLVVMDETTCMVDVARFFLTFTRMESCGKCVPCRIGLKAMLDILERITEGRGQAGD
IETLLEMGSTIKKASLCGLGQTAPNPILSTIKYFREEYEAHINDRRCPSNCCKELLLWQVVEEKCVKCGACFKACPVDAI
IWEKGQIAILDKEKCTKCKSCYDACRFMAIE
>Mature_590_residues
GEAIKILICQGTGGVSAGAKKVEAEFLRVIGEKGVDATVGKRCDVIKTGCRGLCANDVLVDIVDPELGRVTYDFVLPEEV
EKIVAEHIVNRTPLEKRKAKPYYNTFVDQQMRVVMTGCGQIDPESLDAYLEEDGFKAIEKCVKEMTPAAVIDEVKKSGLR
GRGGGGFPTGMKWSFCAATPGKLKYVICNADEGDPGAFMDRSILEGDPYCIIEGMMIAAYAIGCTAGYVYVRAEYPLAIE
RLQKALDVCYEKGYLGKNVMGWGFDFDLRIKKGAGAFVCGEETALMASIEGERGMPRPRPPFPAVKGLWGKPTNINNVET
FANVRHIILKGADWYASLGTDTTKGTKIFAVTGKVKHTGLVEVPAGMSVREVIYNVCGGIANNRKFKAVQAGGPSGGCIP
SEVLDTPVDYDSLIKAGAMMGSGGLVVMDETTCMVDVARFFLTFTRMESCGKCVPCRIGLKAMLDILERITEGRGQAGDI
ETLLEMGSTIKKASLCGLGQTAPNPILSTIKYFREEYEAHINDRRCPSNCCKELLLWQVVEEKCVKCGACFKACPVDAII
WEKGQIAILDKEKCTKCKSCYDACRFMAIE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=433, Percent_Identity=38.1062355658199, Blast_Score=306, Evalue=3e-83,
Organism=Homo sapiens, GI20149568, Length=426, Percent_Identity=38.4976525821596, Blast_Score=305, Evalue=5e-83,
Organism=Escherichia coli, GI1788620, Length=402, Percent_Identity=42.0398009950249, Blast_Score=346, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI17531727, Length=453, Percent_Identity=36.8653421633554, Blast_Score=301, Evalue=5e-82,
Organism=Drosophila melanogaster, GI19920794, Length=376, Percent_Identity=40.9574468085106, Blast_Score=305, Evalue=5e-83,
Organism=Drosophila melanogaster, GI45550452, Length=389, Percent_Identity=40.8740359897172, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24653819, Length=383, Percent_Identity=32.3759791122715, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24653817, Length=355, Percent_Identity=30.4225352112676, Blast_Score=176, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 64238; Mature: 64107

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00645 COMPLEX1_51K_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
5.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEAIKILICQGTGGVSAGAKKVEAEFLRVIGEKGVDATVGKRCDVIKTGCRGLCANDVL
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHCCCCCCCCCEE
VDIVDPELGRVTYDFVLPEEVEKIVAEHIVNRTPLEKRKAKPYYNTFVDQQMRVVMTGCG
EEECCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHCCC
QIDPESLDAYLEEDGFKAIEKCVKEMTPAAVIDEVKKSGLRGRGGGGFPTGMKWSFCAAT
CCCHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECC
PGKLKYVICNADEGDPGAFMDRSILEGDPYCIIEGMMIAAYAIGCTAGYVYVRAEYPLAI
CCCEEEEEEECCCCCCCCHHCCHHHCCCCEEEEHHHHHHHHHHHHCEEEEEEEECCCHHH
ERLQKALDVCYEKGYLGKNVMGWGFDFDLRIKKGAGAFVCGEETALMASIEGERGMPRPR
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCEEEEEECCCCCCCCCC
PPFPAVKGLWGKPTNINNVETFANVRHIILKGADWYASLGTDTTKGTKIFAVTGKVKHTG
CCCHHHCCCCCCCCCCCCHHHHHHHHHHEEECCCHHEECCCCCCCCCEEEEEECCCCCCC
LVEVPAGMSVREVIYNVCGGIANNRKFKAVQAGGPSGGCIPSEVLDTPVDYDSLIKAGAM
EEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCCHHHHHHHHHE
MGSGGLVVMDETTCMVDVARFFLTFTRMESCGKCVPCRIGLKAMLDILERITEGRGQAGD
ECCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHCCCCCCCC
IETLLEMGSTIKKASLCGLGQTAPNPILSTIKYFREEYEAHINDRRCPSNCCKELLLWQV
HHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VEEKCVKCGACFKACPVDAIIWEKGQIAILDKEKCTKCKSCYDACRFMAIE
HHHHHHHHHHHHHHCCCCEEEECCCCEEEECHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GEAIKILICQGTGGVSAGAKKVEAEFLRVIGEKGVDATVGKRCDVIKTGCRGLCANDVL
CCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHCCCCHHHHHCCCCCCCCCEE
VDIVDPELGRVTYDFVLPEEVEKIVAEHIVNRTPLEKRKAKPYYNTFVDQQMRVVMTGCG
EEECCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHCCC
QIDPESLDAYLEEDGFKAIEKCVKEMTPAAVIDEVKKSGLRGRGGGGFPTGMKWSFCAAT
CCCHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECC
PGKLKYVICNADEGDPGAFMDRSILEGDPYCIIEGMMIAAYAIGCTAGYVYVRAEYPLAI
CCCEEEEEEECCCCCCCCHHCCHHHCCCCEEEEHHHHHHHHHHHHCEEEEEEEECCCHHH
ERLQKALDVCYEKGYLGKNVMGWGFDFDLRIKKGAGAFVCGEETALMASIEGERGMPRPR
HHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCEEEEEECCCCCCCCCC
PPFPAVKGLWGKPTNINNVETFANVRHIILKGADWYASLGTDTTKGTKIFAVTGKVKHTG
CCCHHHCCCCCCCCCCCCHHHHHHHHHHEEECCCHHEECCCCCCCCCEEEEEECCCCCCC
LVEVPAGMSVREVIYNVCGGIANNRKFKAVQAGGPSGGCIPSEVLDTPVDYDSLIKAGAM
EEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHCCCCCHHHHHHHHHE
MGSGGLVVMDETTCMVDVARFFLTFTRMESCGKCVPCRIGLKAMLDILERITEGRGQAGD
ECCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHHHCCCCCCCC
IETLLEMGSTIKKASLCGLGQTAPNPILSTIKYFREEYEAHINDRRCPSNCCKELLLWQV
HHHHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VEEKCVKCGACFKACPVDAIIWEKGQIAILDKEKCTKCKSCYDACRFMAIE
HHHHHHHHHHHHHHCCCCEEEECCCCEEEECHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]