Click here to switch to the map view.

The map label for this gene is mspIM [H]

Identifier: 39995337

GI number: 39995337

Start: 234575

End: 235492

Strand: Direct

Name: mspIM [H]

Synonym: GSU0227

Alternate gene names: 39995337

Gene position: 234575-235492 (Clockwise)

Preceding gene: 39995336

Following gene: 39995339

Centisome position: 6.15

GC content: 69.5

Gene sequence:

>918_bases
ATGAGGGCGGTCGAGCTCTTCTGCGGCATCGGCGGTTTCGCCGCCGCGGTGGAGGGGACGGGCGTCCGCGTGGTGGCGGC
TATGGACCAGGACGAGGCGGCCCTTGCCACTTACCGGCTCAACTTCCCCGGCCACGGCGCGCGGAAGGTGGATCTGGAGC
GGGTGAGCGCCTGGGAGCTGACCGCAGGAGGGGTAGACCTCTGGTGGCTGTCGCCTCCGTGCCAGCCTTACTGCGAACGG
GGCGTCCGCCGGGATCTGGCTGATCCCCGGGCCCGGAGCCTCGTGCATATCCTCAATCTGGCCGCCAGGATGTCCGATGA
GGCTCTGCCGCGCCATCTGGCCCTGGAGAACGTGGCCGGCTTTGTCGGCTCCGAAGCCCACGGCCGGCTCACGGAGGTGC
TGTCGTCGCGGGGGTACCGGCTGCAGGAGCGGCTGCTCTGCCCCACGGAGCTGGGCATTCCCTCCCGTCGCCCCCGCTAC
TATCTGGCCGCCTCCCGGGAGTCCCTGGCACCGGCGGAGGTTCTCTCGCCACTGCCCCGGCAGCCTTTGGCCGAGTACCT
TGATCTCCTGCCGGCAAACGGCCAGCCGGCCGAGCTCCTGCTCTCCCCCGCCATCGTGGAACGGTTCGGCGCGGGCTTCC
GCATTCTCGACCCTGCCGATCCCGATGCCTACACCACCTGCTTCACTTCGGGCTACGGCAGGTCCCTCACGGCATCGGGG
GCCTACCTGCGCTGCAGCGACGGTGTCAGGCGTTTTTCCCCCGAGGAGATCGCCCGCCTGCTGCACTTTCCACCGTCGTT
CCGCTTTCCGGAAGAGGTGCCGCTGCGCAAGCGCTGGCAACTGGTGGGGAACAGTCTGTCCGTGGCCGCGGTGCGGGAGG
TGCTTCGGGCGTTGCCGTTACCGGCTGAGGAGGGGTGA

Upstream 100 bases:

>100_bases
GTCTTCGCACTCCTGCTGACCCTGGTCACCTCCCTGTTCGTGGTGGTTCTGCTCACGGTCAACGGCATGGGGAGAGTTTC
GTGAGCCCGGAGGGGCGGGG

Downstream 100 bases:

>100_bases
TCGGGGAGGGAAATGAAAAAAGGCGGCTCAAGGCCGCCTTTTTTGTGGAGGAGAAGGGAGGAGCTAGAGGATTTCCACCA
GTTGGAACTCGTAGGTGAGG

Product: type II DNA modification methyltransferase

Products: NA

Alternate protein names: M.MspI; Cytosine-specific methyltransferase MspI [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MRAVELFCGIGGFAAAVEGTGVRVVAAMDQDEAALATYRLNFPGHGARKVDLERVSAWELTAGGVDLWWLSPPCQPYCER
GVRRDLADPRARSLVHILNLAARMSDEALPRHLALENVAGFVGSEAHGRLTEVLSSRGYRLQERLLCPTELGIPSRRPRY
YLAASRESLAPAEVLSPLPRQPLAEYLDLLPANGQPAELLLSPAIVERFGAGFRILDPADPDAYTTCFTSGYGRSLTASG
AYLRCSDGVRRFSPEEIARLLHFPPSFRFPEEVPLRKRWQLVGNSLSVAAVREVLRALPLPAEEG

Sequences:

>Translated_305_residues
MRAVELFCGIGGFAAAVEGTGVRVVAAMDQDEAALATYRLNFPGHGARKVDLERVSAWELTAGGVDLWWLSPPCQPYCER
GVRRDLADPRARSLVHILNLAARMSDEALPRHLALENVAGFVGSEAHGRLTEVLSSRGYRLQERLLCPTELGIPSRRPRY
YLAASRESLAPAEVLSPLPRQPLAEYLDLLPANGQPAELLLSPAIVERFGAGFRILDPADPDAYTTCFTSGYGRSLTASG
AYLRCSDGVRRFSPEEIARLLHFPPSFRFPEEVPLRKRWQLVGNSLSVAAVREVLRALPLPAEEG
>Mature_305_residues
MRAVELFCGIGGFAAAVEGTGVRVVAAMDQDEAALATYRLNFPGHGARKVDLERVSAWELTAGGVDLWWLSPPCQPYCER
GVRRDLADPRARSLVHILNLAARMSDEALPRHLALENVAGFVGSEAHGRLTEVLSSRGYRLQERLLCPTELGIPSRRPRY
YLAASRESLAPAEVLSPLPRQPLAEYLDLLPANGQPAELLLSPAIVERFGAGFRILDPADPDAYTTCFTSGYGRSLTASG
AYLRCSDGVRRFSPEEIARLLHFPPSFRFPEEVPLRKRWQLVGNSLSVAAVREVLRALPLPAEEG

Specific function: This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the MspI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4758184, Length=187, Percent_Identity=35.8288770053476, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI116007318, Length=348, Percent_Identity=27.5862068965517, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17137742, Length=324, Percent_Identity=26.8518518518519, Blast_Score=135, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 33442; Mature: 33442

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVELFCGIGGFAAAVEGTGVRVVAAMDQDEAALATYRLNFPGHGARKVDLERVSAWEL
CCCEEEECCCCHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCEEHHHHHCEEEE
TAGGVDLWWLSPPCQPYCERGVRRDLADPRARSLVHILNLAARMSDEALPRHLALENVAG
ECCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
FVGSEAHGRLTEVLSSRGYRLQERLLCPTELGIPSRRPRYYLAASRESLAPAEVLSPLPR
HHCCCCCHHHHHHHHHCCCCHHHHCCCCHHCCCCCCCCCEEEEECCCCCCHHHHHCCCCC
QPLAEYLDLLPANGQPAELLLSPAIVERFGAGFRILDPADPDAYTTCFTSGYGRSLTASG
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCCCEECCC
AYLRCSDGVRRFSPEEIARLLHFPPSFRFPEEVPLRKRWQLVGNSLSVAAVREVLRALPL
CEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCC
PAEEG
CCCCC
>Mature Secondary Structure
MRAVELFCGIGGFAAAVEGTGVRVVAAMDQDEAALATYRLNFPGHGARKVDLERVSAWEL
CCCEEEECCCCHHHHHCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCEEHHHHHCEEEE
TAGGVDLWWLSPPCQPYCERGVRRDLADPRARSLVHILNLAARMSDEALPRHLALENVAG
ECCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
FVGSEAHGRLTEVLSSRGYRLQERLLCPTELGIPSRRPRYYLAASRESLAPAEVLSPLPR
HHCCCCCHHHHHHHHHCCCCHHHHCCCCHHCCCCCCCCCEEEEECCCCCCHHHHHCCCCC
QPLAEYLDLLPANGQPAELLLSPAIVERFGAGFRILDPADPDAYTTCFTSGYGRSLTASG
CHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCCCEECCC
AYLRCSDGVRRFSPEEIARLLHFPPSFRFPEEVPLRKRWQLVGNSLSVAAVREVLRALPL
CEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCC
PAEEG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2471145 [H]