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The map label for this gene is argG [H]

Identifier: 39995264

GI number: 39995264

Start: 170807

End: 172027

Strand: Direct

Name: argG [H]

Synonym: GSU0153

Alternate gene names: 39995264

Gene position: 170807-172027 (Clockwise)

Preceding gene: 39995263

Following gene: 39995265

Centisome position: 4.48

GC content: 62.82

Gene sequence:

>1221_bases
ATGGCCAAAGCGCACAAAGACGTCAAGAAGATCGTCCTTGCCTATTCGGGCGGACTCGACACATCCATCATCCTCAAGTG
GCTCAAGAACGAGTACGGCTGCGAGGTCATCGCCTTCTCCGCCGATCTGGGCCAAGGCGACGAACTGGCGCCGATCCGCG
ACAAGGCCATCGCAACCGGCGCCGACAAGGTCTACATCGACGACCTGAAGGAAGAATTCGTCAAGGACTTCGTATTCCCC
ATGTTCCGCGCCAACGCCATTTACGAGGGGCACTACCTCCTGGGCACCTCCATCGCCCGCCCCCTCATCGCCAAGCGTCA
GATGGAGATCGCGAAGATCGAGGGTGCCGACGCCGTTTCCCACGGCGCCACCGGCAAGGGGAACGACCAGGTCCGCTTCG
AGCTGGCCTACTACCACTTTGACCCGGCCATCACCGTGGTTGCCCCCTGGCGCGAGTGGAAGCTCAACAGCCGCCAGGCC
CTGGTGAACTACGCCAGGAAGAACGGGATCCCGATCCCGGTCACCAAGAAGCGTCCCTGGTCCTCGGACCGCAACCTCCT
CCACATCTCCTTCGAGGGCGGCATCCTGGAGGATACCTGGGCCGAGCCCCCCGAGAACATGTACGTGCTGACCAAGGCGC
CGGAAAAGGCCCCCAACAAGCCCCAGTTCGTGGAGATCGAATTCAAAAACGGCAATGCCGTGGCCGTTGACGGCGAGAAG
ATGAGCCCGGCCCAGCTCCTGGCTCACCTGAACTACATCGGCGGCGAGCACGGTATCGGCCGGGTCGATCTCCTGGAGAA
CCGCTCCGTGGGCATGAAGTCCCGGGGCGTGTACGAGACCCCCGGCGGCACCATCCTGCGCGAGGCCCACTCGGCGGTGG
AGCAGATCACCATGGACCGCGAGGTCATGCGGATCCGCGACTCCCTCATCCCCGAGTACGCCCGCCAGGTCTATGCCGGC
TACTGGTTCTCGCCGGAGCGGGAGATGCTCCAGACCCTGATCGACGATTCCCAGAAGTGCGTGAACGGCGTGGCCCGGGT
GAAACTCTACAAGGGGCACTGCCGTACCGTGGGGCGCAAGTCCGAGACCAACTCTCTCTTCAACCTGGACTTCGCCACCT
TCGAAAAGGATCAGGTCTTCAACCAGGCCGACGCCACCGGCTTCATCAAGATCAACTCCCTGCGGCTGCGGATCAGGTCG
CTCATGCAGGGGAAGAAGTAG

Upstream 100 bases:

>100_bases
CCGTCTGGGACGAGGCCGAAAACCGGCTCCACATTCAGAAAGCCATCATGGCTTCATTGATGAAGTAACCCGATTTTATT
CCGATCAAAAGGAGATCCCC

Downstream 100 bases:

>100_bases
AAAACGCGCCTTTTCCGCACGCTCTGCGTTGCCCTGCGAAACTGCTTGTGCGGCGTACGTTCAGTATGCCTCCGCACCGT
TTCGTGAGTGCCTTGACCGT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MAKAHKDVKKIVLAYSGGLDTSIILKWLKNEYGCEVIAFSADLGQGDELAPIRDKAIATGADKVYIDDLKEEFVKDFVFP
MFRANAIYEGHYLLGTSIARPLIAKRQMEIAKIEGADAVSHGATGKGNDQVRFELAYYHFDPAITVVAPWREWKLNSRQA
LVNYARKNGIPIPVTKKRPWSSDRNLLHISFEGGILEDTWAEPPENMYVLTKAPEKAPNKPQFVEIEFKNGNAVAVDGEK
MSPAQLLAHLNYIGGEHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHSAVEQITMDREVMRIRDSLIPEYARQVYAG
YWFSPEREMLQTLIDDSQKCVNGVARVKLYKGHCRTVGRKSETNSLFNLDFATFEKDQVFNQADATGFIKINSLRLRIRS
LMQGKK

Sequences:

>Translated_406_residues
MAKAHKDVKKIVLAYSGGLDTSIILKWLKNEYGCEVIAFSADLGQGDELAPIRDKAIATGADKVYIDDLKEEFVKDFVFP
MFRANAIYEGHYLLGTSIARPLIAKRQMEIAKIEGADAVSHGATGKGNDQVRFELAYYHFDPAITVVAPWREWKLNSRQA
LVNYARKNGIPIPVTKKRPWSSDRNLLHISFEGGILEDTWAEPPENMYVLTKAPEKAPNKPQFVEIEFKNGNAVAVDGEK
MSPAQLLAHLNYIGGEHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHSAVEQITMDREVMRIRDSLIPEYARQVYAG
YWFSPEREMLQTLIDDSQKCVNGVARVKLYKGHCRTVGRKSETNSLFNLDFATFEKDQVFNQADATGFIKINSLRLRIRS
LMQGKK
>Mature_405_residues
AKAHKDVKKIVLAYSGGLDTSIILKWLKNEYGCEVIAFSADLGQGDELAPIRDKAIATGADKVYIDDLKEEFVKDFVFPM
FRANAIYEGHYLLGTSIARPLIAKRQMEIAKIEGADAVSHGATGKGNDQVRFELAYYHFDPAITVVAPWREWKLNSRQAL
VNYARKNGIPIPVTKKRPWSSDRNLLHISFEGGILEDTWAEPPENMYVLTKAPEKAPNKPQFVEIEFKNGNAVAVDGEKM
SPAQLLAHLNYIGGEHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHSAVEQITMDREVMRIRDSLIPEYARQVYAGY
WFSPEREMLQTLIDDSQKCVNGVARVKLYKGHCRTVGRKSETNSLFNLDFATFEKDQVFNQADATGFIKINSLRLRIRSL
MQGKK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=404, Percent_Identity=50.2475247524753, Blast_Score=384, Evalue=1e-107,
Organism=Homo sapiens, GI16950633, Length=404, Percent_Identity=50.2475247524753, Blast_Score=384, Evalue=1e-107,
Organism=Escherichia coli, GI1789563, Length=404, Percent_Identity=25.4950495049505, Blast_Score=119, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324514, Length=402, Percent_Identity=43.0348258706468, Blast_Score=351, Evalue=2e-97,
Organism=Drosophila melanogaster, GI21358151, Length=401, Percent_Identity=43.8902743142145, Blast_Score=340, Evalue=1e-93,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 45658; Mature: 45527

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAHKDVKKIVLAYSGGLDTSIILKWLKNEYGCEVIAFSADLGQGDELAPIRDKAIATG
CCCHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHEECC
ADKVYIDDLKEEFVKDFVFPMFRANAIYEGHYLLGTSIARPLIAKRQMEIAKIEGADAVS
CCEEEHHHHHHHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHHHHHHHEEECCCCHHC
HGATGKGNDQVRFELAYYHFDPAITVVAPWREWKLNSRQALVNYARKNGIPIPVTKKRPW
CCCCCCCCCEEEEEEEEEEECCEEEEEECCHHHCCCCHHHHHHHHHHCCCCCCCCCCCCC
SSDRNLLHISFEGGILEDTWAEPPENMYVLTKAPEKAPNKPQFVEIEFKNGNAVAVDGEK
CCCCCEEEEEECCCEECCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEECCCC
MSPAQLLAHLNYIGGEHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHSAVEQITMDR
CCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHH
EVMRIRDSLIPEYARQVYAGYWFSPEREMLQTLIDDSQKCVNGVARVKLYKGHCRTVGRK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHEEEEECHHHHCCCC
SETNSLFNLDFATFEKDQVFNQADATGFIKINSLRLRIRSLMQGKK
CCCCCEEEEEHHHCCHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AKAHKDVKKIVLAYSGGLDTSIILKWLKNEYGCEVIAFSADLGQGDELAPIRDKAIATG
CCHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHEECC
ADKVYIDDLKEEFVKDFVFPMFRANAIYEGHYLLGTSIARPLIAKRQMEIAKIEGADAVS
CCEEEHHHHHHHHHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHHHHHHHEEECCCCHHC
HGATGKGNDQVRFELAYYHFDPAITVVAPWREWKLNSRQALVNYARKNGIPIPVTKKRPW
CCCCCCCCCEEEEEEEEEEECCEEEEEECCHHHCCCCHHHHHHHHHHCCCCCCCCCCCCC
SSDRNLLHISFEGGILEDTWAEPPENMYVLTKAPEKAPNKPQFVEIEFKNGNAVAVDGEK
CCCCCEEEEEECCCEECCCCCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCEEEECCCC
MSPAQLLAHLNYIGGEHGIGRVDLLENRSVGMKSRGVYETPGGTILREAHSAVEQITMDR
CCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHH
EVMRIRDSLIPEYARQVYAGYWFSPEREMLQTLIDDSQKCVNGVARVKLYKGHCRTVGRK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHEEEEECHHHHCCCC
SETNSLFNLDFATFEKDQVFNQADATGFIKINSLRLRIRSLMQGKK
CCCCCEEEEEHHHCCHHHHHCCCCCCEEEEEHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA