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The map label for this gene is argF

Identifier: 39995263

GI number: 39995263

Start: 169863

End: 170774

Strand: Direct

Name: argF

Synonym: GSU0152

Alternate gene names: 39995263

Gene position: 169863-170774 (Clockwise)

Preceding gene: 39995262

Following gene: 39995264

Centisome position: 4.45

GC content: 64.04

Gene sequence:

>912_bases
ATGATCAGGCACTTTCTGGCCCTCAACCAGTTTACGAAAGAGGAGCTCGACGGGCTCTTCACCCTCAGCCGGGAACTGAA
GGACAAGCAGAAGCAGGGCGTTCCCCACCGGCTGCTGGAGGGGAAGAGCGTGGCCCTCATCTTCGAGAAATCGTCCACCC
GGACCCGCGTCTCCTTTGAGGTGGGAGTCGCCCAACTGGGAGCCCATCCCCTGTTCATCTCCTCGGCCACGTCCCAGATG
GGGCGGGGCGAACCCATCAAGGACACGGCCCGTGTCATGGCCCGCTACTGCGACGGCGTCATGATCCGCACCTACGGCCA
GGAGATCGTTGAGGAGTTCGCCCGCTACTCGTCGGTGCCGGTCATCAACGGCCTCACCGACCTGTTCCATCCCTGCCAGA
TCATGGCCGACCTCTTCACGGTCATCGAATACAAGGGAGGCTATCAGGGACTGAAATTCGCATGGGTCGGCGACGGCAAC
AATATGGCCAACACCTGGATTGAAGCCGCCGCCATCCTGGGCTTCGACCTGGCCCTGGCCTGCCCCACCGGGTACGAGCC
CGACCGGCAGGTCTGGGACTGGGCCCAGCAGCGGGCCACGTCCTCCATCACCATCACCGAGGACCCTGAGGAGGCCGTGC
GCGACGCCGACGTCGTCAACACCGATGTCTGGGCCAGCATGGGGCAGGAGCAGGAGCAGAAGGAGCGTGAGGCGGCGTTC
CAGGGCTACTGCCTCGACGACGCCCTGGTTGCCTTGGCCCGGCCCGACTGCATGGTCCTCCACTGCCTCCCGGCCCACCG
GGGCGAGGAGATCACCGACAGTGTCATCGAAGGCCCCCGCTCCGCCGTCTGGGACGAGGCCGAAAACCGGCTCCACATTC
AGAAAGCCATCATGGCTTCATTGATGAAGTAA

Upstream 100 bases:

>100_bases
AGGACCGGGTACTCCGGTTCGTCCCTCCCCTGGTGGTGACCAAGCAGGAAGTGAATGACATGATCGCCGTTCTCGACGGC
ATCCTGGAGGAAATGACGAA

Downstream 100 bases:

>100_bases
CCCGATTTTATTCCGATCAAAAGGAGATCCCCATGGCCAAAGCGCACAAAGACGTCAAGAAGATCGTCCTTGCCTATTCG
GGCGGACTCGACACATCCAT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MIRHFLALNQFTKEELDGLFTLSRELKDKQKQGVPHRLLEGKSVALIFEKSSTRTRVSFEVGVAQLGAHPLFISSATSQM
GRGEPIKDTARVMARYCDGVMIRTYGQEIVEEFARYSSVPVINGLTDLFHPCQIMADLFTVIEYKGGYQGLKFAWVGDGN
NMANTWIEAAAILGFDLALACPTGYEPDRQVWDWAQQRATSSITITEDPEEAVRDADVVNTDVWASMGQEQEQKEREAAF
QGYCLDDALVALARPDCMVLHCLPAHRGEEITDSVIEGPRSAVWDEAENRLHIQKAIMASLMK

Sequences:

>Translated_303_residues
MIRHFLALNQFTKEELDGLFTLSRELKDKQKQGVPHRLLEGKSVALIFEKSSTRTRVSFEVGVAQLGAHPLFISSATSQM
GRGEPIKDTARVMARYCDGVMIRTYGQEIVEEFARYSSVPVINGLTDLFHPCQIMADLFTVIEYKGGYQGLKFAWVGDGN
NMANTWIEAAAILGFDLALACPTGYEPDRQVWDWAQQRATSSITITEDPEEAVRDADVVNTDVWASMGQEQEQKEREAAF
QGYCLDDALVALARPDCMVLHCLPAHRGEEITDSVIEGPRSAVWDEAENRLHIQKAIMASLMK
>Mature_303_residues
MIRHFLALNQFTKEELDGLFTLSRELKDKQKQGVPHRLLEGKSVALIFEKSSTRTRVSFEVGVAQLGAHPLFISSATSQM
GRGEPIKDTARVMARYCDGVMIRTYGQEIVEEFARYSSVPVINGLTDLFHPCQIMADLFTVIEYKGGYQGLKFAWVGDGN
NMANTWIEAAAILGFDLALACPTGYEPDRQVWDWAQQRATSSITITEDPEEAVRDADVVNTDVWASMGQEQEQKEREAAF
QGYCLDDALVALARPDCMVLHCLPAHRGEEITDSVIEGPRSAVWDEAENRLHIQKAIMASLMK

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=304, Percent_Identity=44.4078947368421, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=29.8701298701299, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=43.1192660550459, Blast_Score=243, Evalue=8e-66,
Organism=Escherichia coli, GI1786469, Length=326, Percent_Identity=41.7177914110429, Blast_Score=241, Evalue=4e-65,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=27.2435897435897, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI48994908, Length=359, Percent_Identity=23.9554317548747, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193204318, Length=322, Percent_Identity=25.4658385093168, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322373, Length=309, Percent_Identity=34.3042071197411, Blast_Score=193, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6322331, Length=307, Percent_Identity=26.0586319218241, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24642586, Length=315, Percent_Identity=27.9365079365079, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_GEOSL (Q74GU2)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951214.1
- ProteinModelPortal:   Q74GU2
- SMR:   Q74GU2
- GeneID:   2687999
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0152
- NMPDR:   fig|243231.1.peg.152
- TIGR:   GSU0152
- HOGENOM:   HBG579429
- OMA:   FTVAMYQ
- ProtClustDB:   PRK00779
- BioCyc:   GSUL243231:GSU_0152-MONOMER
- BRENDA:   2.1.3.3
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 33863; Mature: 33863

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 103-103 BINDING 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRHFLALNQFTKEELDGLFTLSRELKDKQKQGVPHRLLEGKSVALIFEKSSTRTRVSFE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCCEEEEEE
VGVAQLGAHPLFISSATSQMGRGEPIKDTARVMARYCDGVMIRTYGQEIVEEFARYSSVP
HHHHHHCCCCEEEEHHHHHHCCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCC
VINGLTDLFHPCQIMADLFTVIEYKGGYQGLKFAWVGDGNNMANTWIEAAAILGFDLALA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHEEE
CPTGYEPDRQVWDWAQQRATSSITITEDPEEAVRDADVVNTDVWASMGQEQEQKEREAAF
CCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHH
QGYCLDDALVALARPDCMVLHCLPAHRGEEITDSVIEGPRSAVWDEAENRLHIQKAIMAS
CCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LMK
HCC
>Mature Secondary Structure
MIRHFLALNQFTKEELDGLFTLSRELKDKQKQGVPHRLLEGKSVALIFEKSSTRTRVSFE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEECCCCCEEEEEE
VGVAQLGAHPLFISSATSQMGRGEPIKDTARVMARYCDGVMIRTYGQEIVEEFARYSSVP
HHHHHHCCCCEEEEHHHHHHCCCCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCC
VINGLTDLFHPCQIMADLFTVIEYKGGYQGLKFAWVGDGNNMANTWIEAAAILGFDLALA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHEEE
CPTGYEPDRQVWDWAQQRATSSITITEDPEEAVRDADVVNTDVWASMGQEQEQKEREAAF
CCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCHHHHHHCCCHHHHHHHHHHH
QGYCLDDALVALARPDCMVLHCLPAHRGEEITDSVIEGPRSAVWDEAENRLHIQKAIMAS
CCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LMK
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA