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The map label for this gene is pilT-1 [H]

Identifier: 39995257

GI number: 39995257

Start: 162069

End: 163229

Strand: Direct

Name: pilT-1 [H]

Synonym: GSU0146

Alternate gene names: 39995257

Gene position: 162069-163229 (Clockwise)

Preceding gene: 39995256

Following gene: 39995258

Centisome position: 4.25

GC content: 63.82

Gene sequence:

>1161_bases
ATGGAACTGAACGATATCCTCACCGTCGCGGTTCGCGCCAAGGCGTCCGACGTCCACATCAAAACGGGCCTCCCGCCCGT
CGTGCGGATCGACGGCCGCCTGCGGCCAATCCCGAACGCGCCGCGGCTGGCGCCGGACCAAGTGCGCGCCATGGCGCTCG
CCATCATGAACGATCGGCAGAAGCGTCTCTTCGAGGAGCACTTCGAATGCGACACTGCCTACGGCGTGCCGGGCCTGGGC
CGCTTCCGGGTGAGCGTTTACTCCCAGCGCGGCACGGTGGCAATGGTGTTTCGCTTCATTCCCTTCGGCATTCCCTCCAT
GGAGAACCTGACCCTGCCGCCGGTCATTAAAAAACTGGCCATGGAGGAGCGGGGCCTCATTCTCGTCACGGGCACCACGG
GGAGCGGCAAGTCCACCACCCTGGCCGCCATGATCGACTACATCAACGAGCACCGGACCTGCAACATCATTACCGTTGAA
GACCCGGTGGAATTCCTCCATCGCGACAAGAAGAGCATCCTCTCCCAGCGGGAGGTAGGATTCGACACCGTCTCCTTCGC
CACCGCCCTCAAGGGTGCCCTCCGCCAGGACCCGGACGTGATCCTGGTCGGCGAGATGCGCGACCTGGAGACCATCGAGA
CCGCCATGCACGCGGCCGAAACCGGTCACCTGGTCATGTCGACCCTCCACACCCTGGATGCCACCGAGACCATCAACCGG
ATCATCTCGGTCTTCCCTCCCTATCACCAGCGCCAGGTCAGGATCCAGCTCGCCGGCGTGATCAAGGGCGTCGTCTCCCA
GCGCCTGGTCCCCCGCGCCGACGGCAAGGGCCGGGTACCGGCGGTCGAGATCATGATCGGCACCGCCCGGATCAAGGAAT
ACATCGACGACAAGGACAAGACGAAACTCCTCCCCGAAGCCATTGCCCAGGGCTATACCTCGTACGGGATGCAGACCTTC
GACCAGTCCCTGATGCTGCTCTACACCCAGAAGCTCATCACCTACGAGGAGGCGCTCCGCCAGTCGTCCAACCCCGACGA
CTTCGCCCTCAAGGTGTCCGGCATTTCGTCCACCTCCGACAGCACGTGGGACGACTTCGTCCATGACGAGGCTCCCCCTG
CGGAGGGAGAGGGCTCCGTCGAGGGCATCGAGAAGTTCTAG

Upstream 100 bases:

>100_bases
TTTCCTCAAGGAGCGGCCAGAACTGGTGAACGAGATCGAAGGGAAGGTTTACGACGTGGCCGGCATTCCCCGCAAAGGGG
CGAAGGAAGCGGCATAGCCC

Downstream 100 bases:

>100_bases
GCCGGTGGCCCGTGAGGTTGATTTAAGCATGCATTCCCGACCGGAGCGGCGGCGTGACCCGTTGTCCACCGCCCTCGACA
TCCTTTCGCGTCGGGACCAC

Product: twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MELNDILTVAVRAKASDVHIKTGLPPVVRIDGRLRPIPNAPRLAPDQVRAMALAIMNDRQKRLFEEHFECDTAYGVPGLG
RFRVSVYSQRGTVAMVFRFIPFGIPSMENLTLPPVIKKLAMEERGLILVTGTTGSGKSTTLAAMIDYINEHRTCNIITVE
DPVEFLHRDKKSILSQREVGFDTVSFATALKGALRQDPDVILVGEMRDLETIETAMHAAETGHLVMSTLHTLDATETINR
IISVFPPYHQRQVRIQLAGVIKGVVSQRLVPRADGKGRVPAVEIMIGTARIKEYIDDKDKTKLLPEAIAQGYTSYGMQTF
DQSLMLLYTQKLITYEEALRQSSNPDDFALKVSGISSTSDSTWDDFVHDEAPPAEGEGSVEGIEKF

Sequences:

>Translated_386_residues
MELNDILTVAVRAKASDVHIKTGLPPVVRIDGRLRPIPNAPRLAPDQVRAMALAIMNDRQKRLFEEHFECDTAYGVPGLG
RFRVSVYSQRGTVAMVFRFIPFGIPSMENLTLPPVIKKLAMEERGLILVTGTTGSGKSTTLAAMIDYINEHRTCNIITVE
DPVEFLHRDKKSILSQREVGFDTVSFATALKGALRQDPDVILVGEMRDLETIETAMHAAETGHLVMSTLHTLDATETINR
IISVFPPYHQRQVRIQLAGVIKGVVSQRLVPRADGKGRVPAVEIMIGTARIKEYIDDKDKTKLLPEAIAQGYTSYGMQTF
DQSLMLLYTQKLITYEEALRQSSNPDDFALKVSGISSTSDSTWDDFVHDEAPPAEGEGSVEGIEKF
>Mature_386_residues
MELNDILTVAVRAKASDVHIKTGLPPVVRIDGRLRPIPNAPRLAPDQVRAMALAIMNDRQKRLFEEHFECDTAYGVPGLG
RFRVSVYSQRGTVAMVFRFIPFGIPSMENLTLPPVIKKLAMEERGLILVTGTTGSGKSTTLAAMIDYINEHRTCNIITVE
DPVEFLHRDKKSILSQREVGFDTVSFATALKGALRQDPDVILVGEMRDLETIETAMHAAETGHLVMSTLHTLDATETINR
IISVFPPYHQRQVRIQLAGVIKGVVSQRLVPRADGKGRVPAVEIMIGTARIKEYIDDKDKTKLLPEAIAQGYTSYGMQTF
DQSLMLLYTQKLITYEEALRQSSNPDDFALKVSGISSTSDSTWDDFVHDEAPPAEGEGSVEGIEKF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=323, Percent_Identity=41.4860681114551, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1789723, Length=300, Percent_Identity=35, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786296, Length=282, Percent_Identity=34.0425531914894, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42781; Mature: 42781

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELNDILTVAVRAKASDVHIKTGLPPVVRIDGRLRPIPNAPRLAPDQVRAMALAIMNDRQ
CCCHHEEEEEEEECCCCEEEECCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KRLFEEHFECDTAYGVPGLGRFRVSVYSQRGTVAMVFRFIPFGIPSMENLTLPPVIKKLA
HHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHH
MEERGLILVTGTTGSGKSTTLAAMIDYINEHRTCNIITVEDPVEFLHRDKKSILSQREVG
HHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHCC
FDTVSFATALKGALRQDPDVILVGEMRDLETIETAMHAAETGHLVMSTLHTLDATETINR
CHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IISVFPPYHQRQVRIQLAGVIKGVVSQRLVPRADGKGRVPAVEIMIGTARIKEYIDDKDK
HHHHCCCCCHHEEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHCCCHH
TKLLPEAIAQGYTSYGMQTFDQSLMLLYTQKLITYEEALRQSSNPDDFALKVSGISSTSD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCC
STWDDFVHDEAPPAEGEGSVEGIEKF
CCHHHHHCCCCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MELNDILTVAVRAKASDVHIKTGLPPVVRIDGRLRPIPNAPRLAPDQVRAMALAIMNDRQ
CCCHHEEEEEEEECCCCEEEECCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
KRLFEEHFECDTAYGVPGLGRFRVSVYSQRGTVAMVFRFIPFGIPSMENLTLPPVIKKLA
HHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHH
MEERGLILVTGTTGSGKSTTLAAMIDYINEHRTCNIITVEDPVEFLHRDKKSILSQREVG
HHCCCEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHCC
FDTVSFATALKGALRQDPDVILVGEMRDLETIETAMHAAETGHLVMSTLHTLDATETINR
CHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IISVFPPYHQRQVRIQLAGVIKGVVSQRLVPRADGKGRVPAVEIMIGTARIKEYIDDKDK
HHHHCCCCCHHEEEHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHHHHHCCCHH
TKLLPEAIAQGYTSYGMQTFDQSLMLLYTQKLITYEEALRQSSNPDDFALKVSGISSTSD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCC
STWDDFVHDEAPPAEGEGSVEGIEKF
CCHHHHHCCCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]