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The map label for this gene is ligT [C]

Identifier: 39995146

GI number: 39995146

Start: 45707

End: 46246

Strand: Reverse

Name: ligT [C]

Synonym: GSU0035

Alternate gene names: 39995146

Gene position: 46246-45707 (Counterclockwise)

Preceding gene: 39995147

Following gene: 39995140

Centisome position: 1.21

GC content: 62.78

Gene sequence:

>540_bases
ATGTATCGTCTTTTCGTTGCCATTGATCTGCCCGGTTCCGTCAGGGCACAGCTTGCCGATCTCGGCCGGGAACTGGACGG
GGTTCGCCTAGTGCCCCCGGACCAGCTCCACCTGACCCTTCGCTTTATCGGAAATGCCGACGACGAATGCTTTCAGCGGA
TCAAAGACAGCCTGGCTACCGTAACGTCACCACCTTTTACCCTGGCGCTGGCAGGGGTCGGCTGTTTTCCCTCCCCACGA
CGCCCCCGCGTAATCTGGGCCGGGATTACCCCCAATGAGCGGCTGACCCTCCTGCAGCGCGCGGTGGAAAAGGCCGTTGC
AGCGGCGGGTATTCCCCCGGAGGAGCGCCCGTTTTGCCCGCACATAACGCTGGCGCGCCTCAAGGAGGCCATACCGGAGG
GTTTCCCCGCTTTCCAGTCCAGACACGGGGGACTCAGTGCGGAGCCGTTCACTGTCGAAGAATTCCTCCTCTATTCGAGC
ACGCTAACCGCGGGCAGTGCCCTGCACCGTCGCGAGGCGTCCTATCGATTGACCGGCTGA

Upstream 100 bases:

>100_bases
CCCCTTTAGCACGGTCATCACCTCCACCGCTGGCAGCCACCTCGTCGCGCCTGCGGAGGCTAATGCCCGGCTTGCCCGCA
GGCAGTGAATGGAGCAGGCT

Downstream 100 bases:

>100_bases
CCGAGGGAGAAGAGGGGAGGGGAAGAAATGGAAACGGCCGCCATTTCTCAATGAGGATGGCGGCCGTCACAGGCGAACGG
GCACGGCTGTCAGCTGATAA

Product: 2',5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MYRLFVAIDLPGSVRAQLADLGRELDGVRLVPPDQLHLTLRFIGNADDECFQRIKDSLATVTSPPFTLALAGVGCFPSPR
RPRVIWAGITPNERLTLLQRAVEKAVAAAGIPPEERPFCPHITLARLKEAIPEGFPAFQSRHGGLSAEPFTVEEFLLYSS
TLTAGSALHRREASYRLTG

Sequences:

>Translated_179_residues
MYRLFVAIDLPGSVRAQLADLGRELDGVRLVPPDQLHLTLRFIGNADDECFQRIKDSLATVTSPPFTLALAGVGCFPSPR
RPRVIWAGITPNERLTLLQRAVEKAVAAAGIPPEERPFCPHITLARLKEAIPEGFPAFQSRHGGLSAEPFTVEEFLLYSS
TLTAGSALHRREASYRLTG
>Mature_179_residues
MYRLFVAIDLPGSVRAQLADLGRELDGVRLVPPDQLHLTLRFIGNADDECFQRIKDSLATVTSPPFTLALAGVGCFPSPR
RPRVIWAGITPNERLTLLQRAVEKAVAAAGIPPEERPFCPHITLARLKEAIPEGFPAFQSRHGGLSAEPFTVEEFLLYSS
TLTAGSALHRREASYRLTG

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

Organism=Escherichia coli, GI87081692, Length=168, Percent_Identity=33.9285714285714, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 19618; Mature: 19618

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRLFVAIDLPGSVRAQLADLGRELDGVRLVPPDQLHLTLRFIGNADDECFQRIKDSLAT
CEEEEEEEECCCHHHHHHHHHHHHCCCCEECCCCCEEEEEEEECCCCHHHHHHHHHHHHH
VTSPPFTLALAGVGCFPSPRRPRVIWAGITPNERLTLLQRAVEKAVAAAGIPPEERPFCP
CCCCCEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
HITLARLKEAIPEGFPAFQSRHGGLSAEPFTVEEFLLYSSTLTAGSALHRREASYRLTG
CHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCC
>Mature Secondary Structure
MYRLFVAIDLPGSVRAQLADLGRELDGVRLVPPDQLHLTLRFIGNADDECFQRIKDSLAT
CEEEEEEEECCCHHHHHHHHHHHHCCCCEECCCCCEEEEEEEECCCCHHHHHHHHHHHHH
VTSPPFTLALAGVGCFPSPRRPRVIWAGITPNERLTLLQRAVEKAVAAAGIPPEERPFCP
CCCCCEEEEEECCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
HITLARLKEAIPEGFPAFQSRHGGLSAEPFTVEEFLLYSSTLTAGSALHRREASYRLTG
CHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]