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The map label for this gene is grpE

Identifier: 39995143

GI number: 39995143

Start: 41789

End: 42391

Strand: Direct

Name: grpE

Synonym: GSU0032

Alternate gene names: 39995143

Gene position: 41789-42391 (Clockwise)

Preceding gene: 39995142

Following gene: 39995144

Centisome position: 1.1

GC content: 61.53

Gene sequence:

>603_bases
GTGGATAAAAAGAAACACGGCACCGTACAGAGTGCAGATGAAACCATCGCGGCCGCCGGGTTTGCCGGCGAAGCCGCTCA
GGGGAAAGGCGAAGACGTGCAATCCGTTGCCGCCGAGGAGCGGATCAGGCAGCTTGAGACCGAACTGGCGGCCAAGGAGG
CCGAGGCCGCTGCGAATTGGGACAAATTCGTGCGCGAGCGGGCCGACCTGGAGAACTATCGCCGTCGAACCCAGAAAGAA
AAGGAAGAGCTCCTCAAGTACGGCAACGAGAGCCTCCTGCAAGACATCCTGCCAGTCGTGGACAGTATGGAGCGCGCCCT
GGGCCACGCGGACAGCGAGAGCCTGTCCGCGGTTATCGAAGGGATCAGGATGACCCACGGCATGCTGTTGGGCACCCTGA
AGAAATTCGGCGTGGTCGCCGTGGAGGCCGAGCGGGGAACTGTCTTCGATCCGGCGTACCACCAAGCCATGTGCCAGGTT
GAGGTTTCCGAGCTCCCCCCCAATACGGTCGTCGAGGTCTTTCAGAGGGGGTATCTCCTGAATGAACGGCTTCTTCGCCC
CGCCATGGTATCCGTGGCCACAGTGCCCAAGGATGCCGCGTAA

Upstream 100 bases:

>100_bases
GGTGATCCCCATCGTCGACTATACCGCGAAGCTGGTCAGCAGGCTCCTGGAAGGGGAATAGGAGCGGTTCGGCAGATATT
CGCAGGGAGGAACTATCAGC

Downstream 100 bases:

>100_bases
AAAATTCCGCCGGCCCCTTGCTTTTGGGGAGGGCGATGACTACGTTGTTTCCAGAGAGAGACCACAAGGAGGATTATATT
CATGAGCAAAGTGATCGGTA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MDKKKHGTVQSADETIAAAGFAGEAAQGKGEDVQSVAAEERIRQLETELAAKEAEAAANWDKFVRERADLENYRRRTQKE
KEELLKYGNESLLQDILPVVDSMERALGHADSESLSAVIEGIRMTHGMLLGTLKKFGVVAVEAERGTVFDPAYHQAMCQV
EVSELPPNTVVEVFQRGYLLNERLLRPAMVSVATVPKDAA

Sequences:

>Translated_200_residues
MDKKKHGTVQSADETIAAAGFAGEAAQGKGEDVQSVAAEERIRQLETELAAKEAEAAANWDKFVRERADLENYRRRTQKE
KEELLKYGNESLLQDILPVVDSMERALGHADSESLSAVIEGIRMTHGMLLGTLKKFGVVAVEAERGTVFDPAYHQAMCQV
EVSELPPNTVVEVFQRGYLLNERLLRPAMVSVATVPKDAA
>Mature_200_residues
MDKKKHGTVQSADETIAAAGFAGEAAQGKGEDVQSVAAEERIRQLETELAAKEAEAAANWDKFVRERADLENYRRRTQKE
KEELLKYGNESLLQDILPVVDSMERALGHADSESLSAVIEGIRMTHGMLLGTLKKFGVVAVEAERGTVFDPAYHQAMCQV
EVSELPPNTVVEVFQRGYLLNERLLRPAMVSVATVPKDAA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=139, Percent_Identity=35.2517985611511, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1788967, Length=157, Percent_Identity=38.8535031847134, Blast_Score=111, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17552458, Length=151, Percent_Identity=31.7880794701987, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=176, Percent_Identity=31.25, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24653432, Length=161, Percent_Identity=36.6459627329193, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_GEOSL (Q74H60)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_951094.1
- ProteinModelPortal:   Q74H60
- SMR:   Q74H60
- GeneID:   2688590
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU0032
- NMPDR:   fig|243231.1.peg.32
- TIGR:   GSU0032
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- ProtClustDB:   PRK14158
- BioCyc:   GSUL243231:GSU_0032-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21997; Mature: 21997

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKKHGTVQSADETIAAAGFAGEAAQGKGEDVQSVAAEERIRQLETELAAKEAEAAANW
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
DKFVRERADLENYRRRTQKEKEELLKYGNESLLQDILPVVDSMERALGHADSESLSAVIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
GIRMTHGMLLGTLKKFGVVAVEAERGTVFDPAYHQAMCQVEVSELPPNTVVEVFQRGYLL
HHHHHHHHHHHHHHHHCEEEEECCCCCEECHHHHHHHHHHHHHHCCCHHHHHHHHHCHHH
NERLLRPAMVSVATVPKDAA
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDKKKHGTVQSADETIAAAGFAGEAAQGKGEDVQSVAAEERIRQLETELAAKEAEAAANW
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
DKFVRERADLENYRRRTQKEKEELLKYGNESLLQDILPVVDSMERALGHADSESLSAVIE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
GIRMTHGMLLGTLKKFGVVAVEAERGTVFDPAYHQAMCQVEVSELPPNTVVEVFQRGYLL
HHHHHHHHHHHHHHHHCEEEEECCCCCEECHHHHHHHHHHHHHHCCCHHHHHHHHHCHHH
NERLLRPAMVSVATVPKDAA
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA