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The map label for this gene is oprM [H]

Identifier: 39546293

GI number: 39546293

Start: 392419

End: 393876

Strand: Reverse

Name: oprM [H]

Synonym: STM0350.S

Alternate gene names: 39546293

Gene position: 393876-392419 (Counterclockwise)

Preceding gene: 16763731

Following gene: 16763720

Centisome position: 8.11

GC content: 59.19

Gene sequence:

>1458_bases
ATGAAGATCACTTTTACAGGCTATCGGCAGACCGCCACGCTGGCGACGCTTGCTTTCGTCACCACGCTTGCTGGCTGTAC
GATGGCGCCGAAGCACGAACGCCCCGCATCGCCGACTGCGATGGTCTATCCCTACGCAACGTCAACCGTTTCTGGCGCGC
CGGATGCCGCTGACATTGGCTGGCGTGATTTCTTCCACGATCCGCTTCTACAGGAACTGATTGCGATCGCGTTACGCAAT
AATCGGGATTTACGCAAGGCAGGGCTCAATGTTGAAGCCGCCCGGGCGTTATACCGCATTCAGCGCGCGGAGATGCTGCC
GACGCTCGGCATCGCCACCGCCATGGACGCCAGTCGCACTCCCGCCGATCTCAGCGTCATGGACGAGTCTGAGATTAACC
GACGCTACGAGGCGGCTGGGGCGACGACGGCCTGGGAACTGGATCTCTGGGGGCGAGTGCGAAGCCTTAGCGACCAGGCA
TTAGCCGCCTATATGGCGCTTGATGAGACGTACATTGCGGCGCGAATGAGCCTGGTTTCCGAAGTCGCCAGCGCCTGGCT
GACGCTACGGGCTGACCGGGAGCTGCTGCGCTTAACCGAGGATACGCTGGCCGCGCAAAAAAGTTCATACACATTGACGA
CCCAGCTTGCCCGGACAGGTAACGCCACACAGCTCGATCTGCGTATGGCGGAGATCGCGCTGCGTTCTGCCGAAATCAAT
CGCGCGGCGTATACGCGACAGTTGGCGCGGGATCGTAACGCGCTGGAATTGCTGTTGGGCCAGCCGCTCACGCCTGAACT
GTCGCGTCGACTAAACGAAGCGGTCACGCTTACAGAAGGCGCGATCCCGACCACACTGCCAGGCGGATTACCGTCAGATC
TGCTGGTACGCCGCCCGGATATTCGCGCCGCCGAGTACAGGCTGCGCGGCGCAAACGCCCGGATAGGCGCAGCGCGCGCC
GCCTTCTTCCCGACCATCAGCCTGACAGGCTCGGCGGGAACGGCCAGCGCGTCTCTTAGCGGACTCTTTGAACCGGGATC
GGGAAGCTGGCGTTTTCTACCGCAAATCACCTTGCCTCTCTTTCACGGCGGCGCATTACGCGCTGACCTGGATAGGGCGC
ATGTCCAAAAACAGATTGAAATCGCCAGGTATGAAAACGTTATTCAGCAAGCCTTTCGCGACGTGGCGGATGGTCTGGCG
GGACAGCGTACGCTGAATGACCAGGTGCAATCAGAACAACGCGCGGTTGAAGCCAGTCAAATCGCCTATGAGCTGGCCGG
ACTCCGTTTTCAGGAAGGCGTCGATGACTACCTTACGCTGCTTGATACCCATCGTATGCTTTATGGCGCACAACAGCGCC
TGGTACGCACACGTCTCATGCAACAGTTAAATATCATTAACCTGTATAAAGCATTAGGCGGCGGTTGGCGGGAATACAGT
GAGAAAAAGCAAGGTTAG

Upstream 100 bases:

>100_bases
TTGACGCCTGTATTTTACGTGGTGGTTCGCAGGATGGCATTAAAGCGTGAGAACCGCGTTGATTCGCATGATCAGCAAGC
ATAATGATGGGAATGGCATA

Downstream 100 bases:

>100_bases
CCCAAATAGCGCCCGCGTCGCCAGTGCGGCGCGCGCGCTAAACAGGCATATATTACCTGCAAAATAAGGTTAACGAGGGC
AACGATAAACGTTCCCGCTT

Product: outer membrane efflux-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MKITFTGYRQTATLATLAFVTTLAGCTMAPKHERPASPTAMVYPYATSTVSGAPDAADIGWRDFFHDPLLQELIAIALRN
NRDLRKAGLNVEAARALYRIQRAEMLPTLGIATAMDASRTPADLSVMDESEINRRYEAAGATTAWELDLWGRVRSLSDQA
LAAYMALDETYIAARMSLVSEVASAWLTLRADRELLRLTEDTLAAQKSSYTLTTQLARTGNATQLDLRMAEIALRSAEIN
RAAYTRQLARDRNALELLLGQPLTPELSRRLNEAVTLTEGAIPTTLPGGLPSDLLVRRPDIRAAEYRLRGANARIGAARA
AFFPTISLTGSAGTASASLSGLFEPGSGSWRFLPQITLPLFHGGALRADLDRAHVQKQIEIARYENVIQQAFRDVADGLA
GQRTLNDQVQSEQRAVEASQIAYELAGLRFQEGVDDYLTLLDTHRMLYGAQQRLVRTRLMQQLNIINLYKALGGGWREYS
EKKQG

Sequences:

>Translated_485_residues
MKITFTGYRQTATLATLAFVTTLAGCTMAPKHERPASPTAMVYPYATSTVSGAPDAADIGWRDFFHDPLLQELIAIALRN
NRDLRKAGLNVEAARALYRIQRAEMLPTLGIATAMDASRTPADLSVMDESEINRRYEAAGATTAWELDLWGRVRSLSDQA
LAAYMALDETYIAARMSLVSEVASAWLTLRADRELLRLTEDTLAAQKSSYTLTTQLARTGNATQLDLRMAEIALRSAEIN
RAAYTRQLARDRNALELLLGQPLTPELSRRLNEAVTLTEGAIPTTLPGGLPSDLLVRRPDIRAAEYRLRGANARIGAARA
AFFPTISLTGSAGTASASLSGLFEPGSGSWRFLPQITLPLFHGGALRADLDRAHVQKQIEIARYENVIQQAFRDVADGLA
GQRTLNDQVQSEQRAVEASQIAYELAGLRFQEGVDDYLTLLDTHRMLYGAQQRLVRTRLMQQLNIINLYKALGGGWREYS
EKKQG
>Mature_485_residues
MKITFTGYRQTATLATLAFVTTLAGCTMAPKHERPASPTAMVYPYATSTVSGAPDAADIGWRDFFHDPLLQELIAIALRN
NRDLRKAGLNVEAARALYRIQRAEMLPTLGIATAMDASRTPADLSVMDESEINRRYEAAGATTAWELDLWGRVRSLSDQA
LAAYMALDETYIAARMSLVSEVASAWLTLRADRELLRLTEDTLAAQKSSYTLTTQLARTGNATQLDLRMAEIALRSAEIN
RAAYTRQLARDRNALELLLGQPLTPELSRRLNEAVTLTEGAIPTTLPGGLPSDLLVRRPDIRAAEYRLRGANARIGAARA
AFFPTISLTGSAGTASASLSGLFEPGSGSWRFLPQITLPLFHGGALRADLDRAHVQKQIEIARYENVIQQAFRDVADGLA
GQRTLNDQVQSEQRAVEASQIAYELAGLRFQEGVDDYLTLLDTHRMLYGAQQRLVRTRLMQQLNIINLYKALGGGWREYS
EKKQG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=463, Percent_Identity=37.5809935205184, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI2367348, Length=489, Percent_Identity=25.7668711656442, Blast_Score=112, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53401; Mature: 53401

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITFTGYRQTATLATLAFVTTLAGCTMAPKHERPASPTAMVYPYATSTVSGAPDAADIG
CEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
WRDFFHDPLLQELIAIALRNNRDLRKAGLNVEAARALYRIQRAEMLPTLGIATAMDASRT
HHHHHCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCC
PADLSVMDESEINRRYEAAGATTAWELDLWGRVRSLSDQALAAYMALDETYIAARMSLVS
CCCCEECCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVASAWLTLRADRELLRLTEDTLAAQKSSYTLTTQLARTGNATQLDLRMAEIALRSAEIN
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCCEEHHHHHHHHHHHHHHH
RAAYTRQLARDRNALELLLGQPLTPELSRRLNEAVTLTEGAIPTTLPGGLPSDLLVRRPD
HHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHEECCC
IRAAEYRLRGANARIGAARAAFFPTISLTGSAGTASASLSGLFEPGSGSWRFLPQITLPL
CCHHHHEECCCCCCCCHHHHHHCCEEEECCCCCCCCHHHCCCCCCCCCCEEEEEEEECHH
FHGGALRADLDRAHVQKQIEIARYENVIQQAFRDVADGLAGQRTLNDQVQSEQRAVEASQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IAYELAGLRFQEGVDDYLTLLDTHRMLYGAQQRLVRTRLMQQLNIINLYKALGGGWREYS
HHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
EKKQG
HHCCC
>Mature Secondary Structure
MKITFTGYRQTATLATLAFVTTLAGCTMAPKHERPASPTAMVYPYATSTVSGAPDAADIG
CEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCC
WRDFFHDPLLQELIAIALRNNRDLRKAGLNVEAARALYRIQRAEMLPTLGIATAMDASRT
HHHHHCCHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCC
PADLSVMDESEINRRYEAAGATTAWELDLWGRVRSLSDQALAAYMALDETYIAARMSLVS
CCCCEECCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVASAWLTLRADRELLRLTEDTLAAQKSSYTLTTQLARTGNATQLDLRMAEIALRSAEIN
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEHHHHCCCCCEEHHHHHHHHHHHHHHH
RAAYTRQLARDRNALELLLGQPLTPELSRRLNEAVTLTEGAIPTTLPGGLPSDLLVRRPD
HHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHEECCC
IRAAEYRLRGANARIGAARAAFFPTISLTGSAGTASASLSGLFEPGSGSWRFLPQITLPL
CCHHHHEECCCCCCCCHHHHHHCCEEEECCCCCCCCHHHCCCCCCCCCCEEEEEEEECHH
FHGGALRADLDRAHVQKQIEIARYENVIQQAFRDVADGLAGQRTLNDQVQSEQRAVEASQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IAYELAGLRFQEGVDDYLTLLDTHRMLYGAQQRLVRTRLMQQLNIINLYKALGGGWREYS
HHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
EKKQG
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]