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The map label for this gene is hypB3 [H]

Identifier: 38637743

GI number: 38637743

Start: 73016

End: 74032

Strand: Direct

Name: hypB3 [H]

Synonym: PHG078

Alternate gene names: 38637743

Gene position: 73016-74032 (Clockwise)

Preceding gene: 38637742

Following gene: 38637744

Centisome position: 16.15

GC content: 65.19

Gene sequence:

>1017_bases
ATGTGCACGACTTGCGGTTGCGGCGATACGACGGGCGCAGTGGTCTCGGGCCCGGCAGCAGAAGGTGAACCGGCGCCAAC
AGCCGGCGCGGGACAGGACGTGGTGCATGCGCATCCGTATCCGGATGCCATGTCACCCGGGCCCGCGCAGGAGGCCGGCG
GCATGGTGTTGCGCAAGCATCCCGCCGACGATCATGCCGGCCACGCGGGTGCCCTCCTGCATGCGCCCATGCATCGGCGT
GCCATGCATTACCACGACCATCCGCTCGAGCATGCGCACGCTCATTCCCACGAACACGCCCACGAACATGCTCACGAACA
TGCTCACATCCACGGCACCTCCGTCGTCATCGAGCAGGACATCCTCGCCAAGAACCAGTGGATCGCGCAGCGCAATCGCG
GCTGGCTGGCGGGGCGCTCCATTGTTGCGCTGAACCTCGTCAGTTCGCCAGGGGCCGGCAAGACCACGCTGCTGGAACGC
ACCATCCGCGACCTGGGCCCGACGCTGGAGTTGACGGTGATCGAAGGGGATCAGGCCACGCTGAACGACGCGCAGCGCAT
CCGCGCGGCCGGCTGCCGTGCGATCCAGGTCAATACCGGCACGGGCTGCCACCTGGATGCAGACATGATCTCGCGCGCGC
TGACCGCTCTCGATCCGCCGCCGCATTCAGTTGTCATGATCGAGAATGTGGGCAACCTTATCTGTCCGGCCCTGTTCGAC
CTTGGGGAGCAGGCCAAGGTGGTGATCCTCTCCGTGACCGAAGGCGAAGACAAGCCGCTTAAGTATCCACACATGTTCCG
TGCCAGCAGCCTGATGCTGATGACCAAGACGGACCTGCTGCCGTACGTGAGCTTCGACGTGGCACGCTGCCTCGCCTGTG
CGCGCCAGGTCAATCCGGACATCGCGATCCTACAGGTGTCGGCGCTGTCCGGCGCGGGAATGGATGACTGGTATCTCTGG
CTTCGTACCGCCTGCGCAGGGCGTATCGCGCCGCCTGAGGACTCGTTTCCGGCGTGA

Upstream 100 bases:

>100_bases
TGGAGATCGCGGTGCCGTTCGGGCAGTGCGCGTGCGGCAGCGAGAACCTGGAGTTGATCTCCGGGCAGCAGTTGAAGATC
CGGCGCATGGAGGTGGCGTG

Downstream 100 bases:

>100_bases
CCAGGGCATCGCTGGGCGGCACGTACCGGGTCGTGACTGTACAAATGGAGTGATCCATCTGTCAGAAATGCCAGGTTCAT
GGTGTAGCGCGCCACGCCGT

Product: HypB3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MCTTCGCGDTTGAVVSGPAAEGEPAPTAGAGQDVVHAHPYPDAMSPGPAQEAGGMVLRKHPADDHAGHAGALLHAPMHRR
AMHYHDHPLEHAHAHSHEHAHEHAHEHAHIHGTSVVIEQDILAKNQWIAQRNRGWLAGRSIVALNLVSSPGAGKTTLLER
TIRDLGPTLELTVIEGDQATLNDAQRIRAAGCRAIQVNTGTGCHLDADMISRALTALDPPPHSVVMIENVGNLICPALFD
LGEQAKVVILSVTEGEDKPLKYPHMFRASSLMLMTKTDLLPYVSFDVARCLACARQVNPDIAILQVSALSGAGMDDWYLW
LRTACAGRIAPPEDSFPA

Sequences:

>Translated_338_residues
MCTTCGCGDTTGAVVSGPAAEGEPAPTAGAGQDVVHAHPYPDAMSPGPAQEAGGMVLRKHPADDHAGHAGALLHAPMHRR
AMHYHDHPLEHAHAHSHEHAHEHAHEHAHIHGTSVVIEQDILAKNQWIAQRNRGWLAGRSIVALNLVSSPGAGKTTLLER
TIRDLGPTLELTVIEGDQATLNDAQRIRAAGCRAIQVNTGTGCHLDADMISRALTALDPPPHSVVMIENVGNLICPALFD
LGEQAKVVILSVTEGEDKPLKYPHMFRASSLMLMTKTDLLPYVSFDVARCLACARQVNPDIAILQVSALSGAGMDDWYLW
LRTACAGRIAPPEDSFPA
>Mature_338_residues
MCTTCGCGDTTGAVVSGPAAEGEPAPTAGAGQDVVHAHPYPDAMSPGPAQEAGGMVLRKHPADDHAGHAGALLHAPMHRR
AMHYHDHPLEHAHAHSHEHAHEHAHEHAHIHGTSVVIEQDILAKNQWIAQRNRGWLAGRSIVALNLVSSPGAGKTTLLER
TIRDLGPTLELTVIEGDQATLNDAQRIRAAGCRAIQVNTGTGCHLDADMISRALTALDPPPHSVVMIENVGNLICPALFD
LGEQAKVVILSVTEGEDKPLKYPHMFRASSLMLMTKTDLLPYVSFDVARCLACARQVNPDIAILQVSALSGAGMDDWYLW
LRTACAGRIAPPEDSFPA

Specific function: Required for the maturation of the three [NiFe]hydrogenases. Exhibits a low intrinsic GTPase activity. The GTP hydrolysis catalyzed by HypB is an integral process in the incorporation of nickel into hydrogenases [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=327, Percent_Identity=47.7064220183486, Blast_Score=271, Evalue=4e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 36074; Mature: 36074

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCTTCGCGDTTGAVVSGPAAEGEPAPTAGAGQDVVHAHPYPDAMSPGPAQEAGGMVLRKH
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHCCEEEEEC
PADDHAGHAGALLHAPMHRRAMHYHDHPLEHAHAHSHEHAHEHAHEHAHIHGTSVVIEQD
CCCCCCCCCCHHEECCHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCCEECCCEEEEEHH
ILAKNQWIAQRNRGWLAGRSIVALNLVSSPGAGKTTLLERTIRDLGPTLELTVIEGDQAT
HHHHHHHHHHCCCCEECCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCH
LNDAQRIRAAGCRAIQVNTGTGCHLDADMISRALTALDPPPHSVVMIENVGNLICPALFD
HHHHHHHHHCCCEEEEEECCCCCEECHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHC
LGEQAKVVILSVTEGEDKPLKYPHMFRASSLMLMTKTDLLPYVSFDVARCLACARQVNPD
CCCCCEEEEEEEECCCCCCCCCCHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
IAILQVSALSGAGMDDWYLWLRTACAGRIAPPEDSFPA
EEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MCTTCGCGDTTGAVVSGPAAEGEPAPTAGAGQDVVHAHPYPDAMSPGPAQEAGGMVLRKH
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHCCEEEEEC
PADDHAGHAGALLHAPMHRRAMHYHDHPLEHAHAHSHEHAHEHAHEHAHIHGTSVVIEQD
CCCCCCCCCCHHEECCHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHCCEECCCEEEEEHH
ILAKNQWIAQRNRGWLAGRSIVALNLVSSPGAGKTTLLERTIRDLGPTLELTVIEGDQAT
HHHHHHHHHHCCCCEECCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCH
LNDAQRIRAAGCRAIQVNTGTGCHLDADMISRALTALDPPPHSVVMIENVGNLICPALFD
HHHHHHHHHCCCEEEEEECCCCCEECHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHC
LGEQAKVVILSVTEGEDKPLKYPHMFRASSLMLMTKTDLLPYVSFDVARCLACARQVNPD
CCCCCEEEEEEEECCCCCCCCCCHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
IAILQVSALSGAGMDDWYLWLRTACAGRIAPPEDSFPA
EEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]