| Definition | Cupriavidus necator megaplasmid pHG1, complete sequence. |
|---|---|
| Accession | NC_005241 |
| Length | 452,156 |
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The map label for this gene is hypF3 [H]
Identifier: 38637737
GI number: 38637737
Start: 66953
End: 69343
Strand: Direct
Name: hypF3 [H]
Synonym: PHG072
Alternate gene names: 38637737
Gene position: 66953-69343 (Clockwise)
Preceding gene: 38637736
Following gene: 38637738
Centisome position: 14.81
GC content: 71.43
Gene sequence:
>2391_bases GTGCGCGGCATGGTGCAGGGGGTCGGGTTCCGGCCCACCGTATGGCGGCTCGCACACGATTGCGGGCTGTCCGGCTCCGT GCGCAACGACAGCGATGGCGTGCAGATCCGCGCCTGGGGCGACGCCGTGGCGCTGGACCGCTTCATTGCGCGGCTGCGGG ACGAGTGCCCGCCCCTCGCGCGCATCGACGCCATCGAGCGCCGCGTCCTGGCGCAGGATGCCGAGGGCGCGGACCTGGGT GGCTTTCGCATTGTTGCCAGCGTGGGCGGGCCGGTGCGGACCGGCGTGGTACCGGACGCCGTGGTCTGTAGTGCCTGCAT CGCCGATATCGGCGACCCGCTGAACCGGCGCTATCGCTATCCGTTTGCCAACTGCACGCATTGCGGCCCGCGCCTGTCGA TCGTGCGCGCGATTCCCTACGACCGCGCCAACACGGCCATGGCCGCGTTCCCGATGTGCCCGGCGTGCCTTGCCGAGTAT GAAGACCCGGCCGACCGCCGCTTCCACGCGCAGCCGCTGGCCTGCGCGGCCTGCGGACCCCACGTGTGGCTCGAGCACAC AGGCGGGCCGCTGGCCGCCGGCCCAGACGACGCGATCCGCGCGGCGTGGGCGCTCCTGCGCGACGGCGGGATCGTCGCCA TCAAGGGCATCGGCGGGTTCCAGTTGGCCTGCGATGCCTGCAACCCCGTGGCGGTGGCGCGCCTGCGCCAGCGCAAGCGG CGCGAGCGCAAGCCGTTCGCACTGATGGCGCGCGACCTGGCGGTGATCCGCCAATACAGCGAGCCGACCGCGGCCGAATG TGGGTTGCTGCAAAGCCCGGCGGCACCCATCGTGATCGTCCAGACGCGCAGCGACCGGCAGGGTGCGCCCCTGGCGCCCG ACGTCGCGCCCGGCATCGCCGCGCTGGGGTTCATGCTGCCGGGCACACCGCTGCACTGGCTGCTGATGGCGATGGCCGAC CGCCCGATCGTGCTGACCAGCGGCAACGCCAGCGACGAGCCCCAGTGCACGGAGAACGCCGACGCGCGCATGCGCCTCGG CGCGATCGCCGATGCGTTCCTGATGCATGACCGTGACGTGGTCCGGCGCGTGGATGACTCAGTCGCGCGCGTCGTGCTGG ACGTGCCCCGGGTGATGCGGCGCTCACGCGGATATGCGCCCGCGCCGCTGCCGTTGCCGCCGGGCTTCGCTGCCGCGGCG CCGGTGCTGGCCATGGGCGGCGAGCTCAAGAACACGTTCTGCCTGATGCGCGCGGGCGAGGCCATCTTGTCCCACCATAT GGGCGACCTGGAAGACGCGCTGACCCATGCCGACTACCGCCGCGCGCTGACGCAATACATGGCGTTGTTCGAGCATGGCC CGCATCTGGTCGCGGTGGACATGCATCCCGAATACCTGTCCGGCAAGATCGGCCGCGACCTGGCACGGCAGGGGCAGCTG CCGGTTGCGCAGGTGCAGCATCATCACGCGCATATCGCCGCGTGCATGGCGGAGAACCGCGTGGCCCTGGCCGCCGGCCC CGTACTGGGCGTGGCGCTGGATGGCATCGGGTACGGTGTCGATGGCAGTCTATGGGGTGGCGAATTCCTGTTGGCGGACT ACCGGGACTTCACGCGGCTGGCCTGCTTCAAGCCCGTGGGGATGCCCGGCGGTGAGCGCGCGATCCACCAGCCGTGGCGC AATACCTACGCACATCTGGCCGCCGCGGTCGGGTGGGATACCCTGATCGCCCGGCATGCCAGCCTGGCGCTTGTACGCTT CCTGGCAACGCGCCCGCGCGAGGTACTGGACGCCATGCTTACGCGCAGCATCAACAGCCCGCTGGCCAGCTCGGCCGGGC GGCTGTTCGATGCGGTGGCGGCGGCCGCTGGCGTCTGTCCCGAGCGGGCATGCTACGAGGGGCAGGCCGCCATGGAGTTC GAAGCGCTGGCCGATCGCCGCACGCTGAGCGAGGAAGACGATGGCCGTGCCTATCCGTTCGCCATCGTGCCGGGCACGCC GGCCTGCCTGGAGCCGGGCCCGATGTGGGTGGCCCTGCTGGCTGACCTGGCGCGCGCTACGCCGGCGCCGCTGATCAGCG CGCGCTTCCACAAGGGGCTGGCGATCGCCATCGCGAGCATGGTCGCGTACCTGGCGGACTGCCCGGGCGCGGGGTGCCGC GCGGTGCCGGTGGCCCTGTCCGGCGGTGTGTTCCAGAACCGCATCCTGCTCGAACAGGTCGCCACGCGGCTTTCCGCGCA AGGCCGCCGCGTGCTGACGCACAGCCAGGTACCCGCCAATGACGGCGGCCTCTCGTTCGGGCAGGCGGCCATCGCCGCCG CGCGGTCCCTGGGAGGCGCAGACGTTGCAGGCCAGGCAAACGCGGGCTATCGGAGTACAACATGTGCCTAG
Upstream 100 bases:
>100_bases AGCTGCGCATCGCGCAGACCAAAGGCACGCCATGCAAGCCTGTGCACTGCCAGTCCGGGACCGGTCCGCCGCGGTACGGC CACGCGGCGAGGAGATCCGC
Downstream 100 bases:
>100_bases GTATCCCCGGACAGATCCTGGAAGTGCGCGACGCCGCCAATGCCTTGGCGATGGTCGAGGTCGGCGGCGTGCGGCGCCTG ATCAATATCGCCTTCATCGT
Product: HypF3
Products: NA
Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]
Number of amino acids: Translated: 796; Mature: 796
Protein sequence:
>796_residues MRGMVQGVGFRPTVWRLAHDCGLSGSVRNDSDGVQIRAWGDAVALDRFIARLRDECPPLARIDAIERRVLAQDAEGADLG GFRIVASVGGPVRTGVVPDAVVCSACIADIGDPLNRRYRYPFANCTHCGPRLSIVRAIPYDRANTAMAAFPMCPACLAEY EDPADRRFHAQPLACAACGPHVWLEHTGGPLAAGPDDAIRAAWALLRDGGIVAIKGIGGFQLACDACNPVAVARLRQRKR RERKPFALMARDLAVIRQYSEPTAAECGLLQSPAAPIVIVQTRSDRQGAPLAPDVAPGIAALGFMLPGTPLHWLLMAMAD RPIVLTSGNASDEPQCTENADARMRLGAIADAFLMHDRDVVRRVDDSVARVVLDVPRVMRRSRGYAPAPLPLPPGFAAAA PVLAMGGELKNTFCLMRAGEAILSHHMGDLEDALTHADYRRALTQYMALFEHGPHLVAVDMHPEYLSGKIGRDLARQGQL PVAQVQHHHAHIAACMAENRVALAAGPVLGVALDGIGYGVDGSLWGGEFLLADYRDFTRLACFKPVGMPGGERAIHQPWR NTYAHLAAAVGWDTLIARHASLALVRFLATRPREVLDAMLTRSINSPLASSAGRLFDAVAAAAGVCPERACYEGQAAMEF EALADRRTLSEEDDGRAYPFAIVPGTPACLEPGPMWVALLADLARATPAPLISARFHKGLAIAIASMVAYLADCPGAGCR AVPVALSGGVFQNRILLEQVATRLSAQGRRVLTHSQVPANDGGLSFGQAAIAAARSLGGADVAGQANAGYRSTTCA
Sequences:
>Translated_796_residues MRGMVQGVGFRPTVWRLAHDCGLSGSVRNDSDGVQIRAWGDAVALDRFIARLRDECPPLARIDAIERRVLAQDAEGADLG GFRIVASVGGPVRTGVVPDAVVCSACIADIGDPLNRRYRYPFANCTHCGPRLSIVRAIPYDRANTAMAAFPMCPACLAEY EDPADRRFHAQPLACAACGPHVWLEHTGGPLAAGPDDAIRAAWALLRDGGIVAIKGIGGFQLACDACNPVAVARLRQRKR RERKPFALMARDLAVIRQYSEPTAAECGLLQSPAAPIVIVQTRSDRQGAPLAPDVAPGIAALGFMLPGTPLHWLLMAMAD RPIVLTSGNASDEPQCTENADARMRLGAIADAFLMHDRDVVRRVDDSVARVVLDVPRVMRRSRGYAPAPLPLPPGFAAAA PVLAMGGELKNTFCLMRAGEAILSHHMGDLEDALTHADYRRALTQYMALFEHGPHLVAVDMHPEYLSGKIGRDLARQGQL PVAQVQHHHAHIAACMAENRVALAAGPVLGVALDGIGYGVDGSLWGGEFLLADYRDFTRLACFKPVGMPGGERAIHQPWR NTYAHLAAAVGWDTLIARHASLALVRFLATRPREVLDAMLTRSINSPLASSAGRLFDAVAAAAGVCPERACYEGQAAMEF EALADRRTLSEEDDGRAYPFAIVPGTPACLEPGPMWVALLADLARATPAPLISARFHKGLAIAIASMVAYLADCPGAGCR AVPVALSGGVFQNRILLEQVATRLSAQGRRVLTHSQVPANDGGLSFGQAAIAAARSLGGADVAGQANAGYRSTTCA >Mature_796_residues MRGMVQGVGFRPTVWRLAHDCGLSGSVRNDSDGVQIRAWGDAVALDRFIARLRDECPPLARIDAIERRVLAQDAEGADLG GFRIVASVGGPVRTGVVPDAVVCSACIADIGDPLNRRYRYPFANCTHCGPRLSIVRAIPYDRANTAMAAFPMCPACLAEY EDPADRRFHAQPLACAACGPHVWLEHTGGPLAAGPDDAIRAAWALLRDGGIVAIKGIGGFQLACDACNPVAVARLRQRKR RERKPFALMARDLAVIRQYSEPTAAECGLLQSPAAPIVIVQTRSDRQGAPLAPDVAPGIAALGFMLPGTPLHWLLMAMAD RPIVLTSGNASDEPQCTENADARMRLGAIADAFLMHDRDVVRRVDDSVARVVLDVPRVMRRSRGYAPAPLPLPPGFAAAA PVLAMGGELKNTFCLMRAGEAILSHHMGDLEDALTHADYRRALTQYMALFEHGPHLVAVDMHPEYLSGKIGRDLARQGQL PVAQVQHHHAHIAACMAENRVALAAGPVLGVALDGIGYGVDGSLWGGEFLLADYRDFTRLACFKPVGMPGGERAIHQPWR NTYAHLAAAVGWDTLIARHASLALVRFLATRPREVLDAMLTRSINSPLASSAGRLFDAVAAAAGVCPERACYEGQAAMEF EALADRRTLSEEDDGRAYPFAIVPGTPACLEPGPMWVALLADLARATPAPLISARFHKGLAIAIASMVAYLADCPGAGCR AVPVALSGGVFQNRILLEQVATRLSAQGRRVLTHSQVPANDGGLSFGQAAIAAARSLGGADVAGQANAGYRSTTCA
Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb
COG id: COG0068
COG function: function code O; Hydrogenase maturation factor
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 YrdC-like domain [H]
Homologues:
Organism=Escherichia coli, GI2367152, Length=781, Percent_Identity=44.3021766965429, Blast_Score=528, Evalue=1e-151,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001792 - InterPro: IPR017968 - InterPro: IPR004421 - InterPro: IPR017945 - InterPro: IPR006070 - InterPro: IPR011125 [H]
Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]
EC number: NA
Molecular weight: Translated: 84619; Mature: 84619
Theoretical pI: Translated: 7.70; Mature: 7.70
Prosite motif: PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.9 %Cys (Translated Protein) 2.8 %Met (Translated Protein) 5.7 %Cys+Met (Translated Protein) 2.9 %Cys (Mature Protein) 2.8 %Met (Mature Protein) 5.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MRGMVQGVGFRPTVWRLAHDCGLSGSVRNDSDGVQIRAWGDAVALDRFIARLRDECPPLA CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCHH RIDAIERRVLAQDAEGADLGGFRIVASVGGPVRTGVVPDAVVCSACIADIGDPLNRRYRY HHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCC PFANCTHCGPRLSIVRAIPYDRANTAMAAFPMCPACLAEYEDPADRRFHAQPLACAACGP CCCCCCCCCCCCEEEEECCCCCCCCHHHHHCCCHHHHHCCCCCHHCCCCCCCCCHHHCCC HVWLEHTGGPLAAGPDDAIRAAWALLRDGGIVAIKGIGGFQLACDACNPVAVARLRQRKR CEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCHHHHHHHHHHHH RERKPFALMARDLAVIRQYSEPTAAECGLLQSPAAPIVIVQTRSDRQGAPLAPDVAPGIA HHCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHH ALGFMLPGTPLHWLLMAMADRPIVLTSGNASDEPQCTENADARMRLGAIADAFLMHDRDV HHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHH VRRVDDSVARVVLDVPRVMRRSRGYAPAPLPLPPGFAAAAPVLAMGGELKNTFCLMRAGE HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHH AILSHHMGDLEDALTHADYRRALTQYMALFEHGPHLVAVDMHPEYLSGKIGRDLARQGQL HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHCCCC PVAQVQHHHAHIAACMAENRVALAAGPVLGVALDGIGYGVDGSLWGGEFLLADYRDFTRL CHHHHHHHHHHHHHHHHCCCEEEEECCHHHEEECCCCCCCCCCCCCCCEEEHHHHHHHHH ACFKPVGMPGGERAIHQPWRNTYAHLAAAVGWDTLIARHASLALVRFLATRPREVLDAML HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH TRSINSPLASSAGRLFDAVAAAAGVCPERACYEGQAAMEFEALADRRTLSEEDDGRAYPF HHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEE AIVPGTPACLEPGPMWVALLADLARATPAPLISARFHKGLAIAIASMVAYLADCPGAGCR EECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE AVPVALSGGVFQNRILLEQVATRLSAQGRRVLTHSQVPANDGGLSFGQAAIAAARSLGGA EEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCC DVAGQANAGYRSTTCA CCCCCCCCCCCCCCCC >Mature Secondary Structure MRGMVQGVGFRPTVWRLAHDCGLSGSVRNDSDGVQIRAWGDAVALDRFIARLRDECPPLA CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCHH RIDAIERRVLAQDAEGADLGGFRIVASVGGPVRTGVVPDAVVCSACIADIGDPLNRRYRY HHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCC PFANCTHCGPRLSIVRAIPYDRANTAMAAFPMCPACLAEYEDPADRRFHAQPLACAACGP CCCCCCCCCCCCEEEEECCCCCCCCHHHHHCCCHHHHHCCCCCHHCCCCCCCCCHHHCCC HVWLEHTGGPLAAGPDDAIRAAWALLRDGGIVAIKGIGGFQLACDACNPVAVARLRQRKR CEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCHHHHHHHHHHHH RERKPFALMARDLAVIRQYSEPTAAECGLLQSPAAPIVIVQTRSDRQGAPLAPDVAPGIA HHCCCHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHH ALGFMLPGTPLHWLLMAMADRPIVLTSGNASDEPQCTENADARMRLGAIADAFLMHDRDV HHHHHCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHH VRRVDDSVARVVLDVPRVMRRSRGYAPAPLPLPPGFAAAAPVLAMGGELKNTFCLMRAGE HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHH AILSHHMGDLEDALTHADYRRALTQYMALFEHGPHLVAVDMHPEYLSGKIGRDLARQGQL HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHCCCC PVAQVQHHHAHIAACMAENRVALAAGPVLGVALDGIGYGVDGSLWGGEFLLADYRDFTRL CHHHHHHHHHHHHHHHHCCCEEEEECCHHHEEECCCCCCCCCCCCCCCEEEHHHHHHHHH ACFKPVGMPGGERAIHQPWRNTYAHLAAAVGWDTLIARHASLALVRFLATRPREVLDAML HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH TRSINSPLASSAGRLFDAVAAAAGVCPERACYEGQAAMEFEALADRRTLSEEDDGRAYPF HHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEE AIVPGTPACLEPGPMWVALLADLARATPAPLISARFHKGLAIAIASMVAYLADCPGAGCR EECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE AVPVALSGGVFQNRILLEQVATRLSAQGRRVLTHSQVPANDGGLSFGQAAIAAARSLGGA EEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCC DVAGQANAGYRSTTCA CCCCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8497190; 9492269 [H]