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The map label for this gene is hoxG

Identifier: 38637670

GI number: 38637670

Start: 1236

End: 3092

Strand: Direct

Name: hoxG

Synonym: PHG002

Alternate gene names: 38637670

Gene position: 1236-3092 (Clockwise)

Preceding gene: 38637669

Following gene: 38637671

Centisome position: 0.27

GC content: 58.27

Gene sequence:

>1857_bases
ATGTCAGCTTACGCAACCCAAGGCTTCAATCTTGACGACCGCGGCCGTCGCATTGTCGTCGATCCCGTCACCCGCATCGA
GGGTCATATGCGCTGCGAGGTGAATGTCGATGCCAACAATGTCATTCGCAACGCTGTTTCCACTGGTACCATGTGGCGCG
GACTGGAAGTGATTCTCAAGGGCCGCGATCCGCGCGACGCCTGGGCGTTCGTAGAACGCATCTGCGGTGTTTGTACCGGT
TGTCACGCGCTTGCGTCGGTGCGTGCCGTGGAAAACGCGCTCGACATCAGAATTCCAAAGAACGCCCATCTGATCCGAGA
GATCATGGCCAAGACGTTGCAGGTGCATGACCATGCGGTGCATTTCTATCACCTGCATGCGCTGGATTGGGTGGATGTCA
TGTCAGCCCTGAAAGCCGACCCGAAGAGGACTTCCGAGTTGCAGCAGTTAGTTTCGCCTGCGCATCCGCTGTCCTCGGCA
GGCTATTTCCGCGATATTCAAAATCGACTCAAGCGCTTTGTCGAGAGTGGTCAGCTTGGCCCTTTCATGAATGGGTACTG
GGGATCCAAGGCTTATGTGCTGCCGCCGGAGGCCAATCTGATGGCGGTCACGCATTATTTGGAAGCGCTGGACCTACAGA
AGGAGTGGGTGAAAATCCACACCATCTTCGGCGGCAAGAATCCGCACCCGAACTACTTGGTCGGTGGCGTGCCGTGCGCG
ATCAATCTCGATGGTATCGGGGCTGCCAGCGCGCCGGTAAATATGGAGCGCTTGAGCTTCGTTAAGGCGCGCATCGACGA
GATCATCGAATTCAATAAGAATGTATACGTGCCAGACGTGCTCGCCATCGGCACACTGTATAAACAGGCCGGGTGGCTGT
ACGGCGGCGGGCTGGCAGCCACCAACGTGCTTGACTACGGCGAGTACCCGAACGTTGCCTACAACAAGAGCACTGACCAA
CTGCCCGGCGGCGCGATCCTCAACGGCAACTGGGACGAAGTATTTCCAGTGGATCCGCGCGACTCCCAACAGGTGCAGGA
ATTCGTGTCGCACAGCTGGTACAAGTATGCCGACGAGAGCGTAGGTCTGCATCCCTGGGACGGCGTGACTGAGCCCAATT
ACGTGCTCGGTGCAAACACTAAGGGTACACGCACGCGCATCGAGCAAATCGACGAGAGCGCGAAGTACTCGTGGATTAAA
TCGCCGCGCTGGCGCGGCCACGCGATGGAGGTAGGGCCGCTGTCGCGCTACATCCTTGCCTATGCCCATGCGCGGAGCGG
CAACAAGTACGCTGAGCGTCCCAAGGAGCAGCTTGAGTACTCCGCGCAGATGATCAACAGTGCGATACCAAAGGCATTGG
GATTGCCAGAAACACAATACACGCTCAAGCAGTTGTTGCCCAGCACGATCGGTCGTACGCTGGCGCGCGCACTCGAGAGC
CAATATTGCGGAGAAATGATGCATAGCGACTGGCATGATCTGGTCGCCAACATCCGGGCGGGCGATACGGCAACCGCCAA
CGTTGACAAGTGGGATCCTGCCACCTGGCCGCTGCAAGCCAAGGGCGTTGGGACCGTCGCTGCGCCGCGCGGCGCTCTCG
GACACTGGATTCGTATCAAGGACGGCCGGATCGAGAACTATCAGTGCGTAGTGCCTACCACGTGGAATGGCAGTCCGCGT
GATTACAAGGGGCAGATCGGCGCATTTGAGGCTTCGCTGATGAACACCCCGATGGTCAACCCGGAGCAGCCGGTGGAAAT
CTTGCGCACGCTGCATTCGTTCGATCCCTGTCTGGCGTGTTCGACTCACGTCATGAGCGCGGAAGGCCAGGAACTCACTA
CAGTCAAGGTGCGATAA

Upstream 100 bases:

>100_bases
CCGCAGCGTCTGCGATCAAGCGTGCGTCGAAGAAGAACGAAACCAGCGGCAGTGAACACTAAGCCGCCGGGGAAACGACT
GAATCAGGAAGATCGAAATA

Downstream 100 bases:

>100_bases
AAGGTGCCGGACCGGCGTCTGGCCAGCGAGCGGATATCGGTTCGGCAACGGCACAAGGAGATAGCATGAGCACAAAAATG
CAGGCGGATCGCATTGCAGA

Product: membrane-bound [NiFe] hydrogenase large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit

Number of amino acids: Translated: 618; Mature: 617

Protein sequence:

>618_residues
MSAYATQGFNLDDRGRRIVVDPVTRIEGHMRCEVNVDANNVIRNAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTG
CHALASVRAVENALDIRIPKNAHLIREIMAKTLQVHDHAVHFYHLHALDWVDVMSALKADPKRTSELQQLVSPAHPLSSA
GYFRDIQNRLKRFVESGQLGPFMNGYWGSKAYVLPPEANLMAVTHYLEALDLQKEWVKIHTIFGGKNPHPNYLVGGVPCA
INLDGIGAASAPVNMERLSFVKARIDEIIEFNKNVYVPDVLAIGTLYKQAGWLYGGGLAATNVLDYGEYPNVAYNKSTDQ
LPGGAILNGNWDEVFPVDPRDSQQVQEFVSHSWYKYADESVGLHPWDGVTEPNYVLGANTKGTRTRIEQIDESAKYSWIK
SPRWRGHAMEVGPLSRYILAYAHARSGNKYAERPKEQLEYSAQMINSAIPKALGLPETQYTLKQLLPSTIGRTLARALES
QYCGEMMHSDWHDLVANIRAGDTATANVDKWDPATWPLQAKGVGTVAAPRGALGHWIRIKDGRIENYQCVVPTTWNGSPR
DYKGQIGAFEASLMNTPMVNPEQPVEILRTLHSFDPCLACSTHVMSAEGQELTTVKVR

Sequences:

>Translated_618_residues
MSAYATQGFNLDDRGRRIVVDPVTRIEGHMRCEVNVDANNVIRNAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTG
CHALASVRAVENALDIRIPKNAHLIREIMAKTLQVHDHAVHFYHLHALDWVDVMSALKADPKRTSELQQLVSPAHPLSSA
GYFRDIQNRLKRFVESGQLGPFMNGYWGSKAYVLPPEANLMAVTHYLEALDLQKEWVKIHTIFGGKNPHPNYLVGGVPCA
INLDGIGAASAPVNMERLSFVKARIDEIIEFNKNVYVPDVLAIGTLYKQAGWLYGGGLAATNVLDYGEYPNVAYNKSTDQ
LPGGAILNGNWDEVFPVDPRDSQQVQEFVSHSWYKYADESVGLHPWDGVTEPNYVLGANTKGTRTRIEQIDESAKYSWIK
SPRWRGHAMEVGPLSRYILAYAHARSGNKYAERPKEQLEYSAQMINSAIPKALGLPETQYTLKQLLPSTIGRTLARALES
QYCGEMMHSDWHDLVANIRAGDTATANVDKWDPATWPLQAKGVGTVAAPRGALGHWIRIKDGRIENYQCVVPTTWNGSPR
DYKGQIGAFEASLMNTPMVNPEQPVEILRTLHSFDPCLACSTHVMSAEGQELTTVKVR
>Mature_617_residues
SAYATQGFNLDDRGRRIVVDPVTRIEGHMRCEVNVDANNVIRNAVSTGTMWRGLEVILKGRDPRDAWAFVERICGVCTGC
HALASVRAVENALDIRIPKNAHLIREIMAKTLQVHDHAVHFYHLHALDWVDVMSALKADPKRTSELQQLVSPAHPLSSAG
YFRDIQNRLKRFVESGQLGPFMNGYWGSKAYVLPPEANLMAVTHYLEALDLQKEWVKIHTIFGGKNPHPNYLVGGVPCAI
NLDGIGAASAPVNMERLSFVKARIDEIIEFNKNVYVPDVLAIGTLYKQAGWLYGGGLAATNVLDYGEYPNVAYNKSTDQL
PGGAILNGNWDEVFPVDPRDSQQVQEFVSHSWYKYADESVGLHPWDGVTEPNYVLGANTKGTRTRIEQIDESAKYSWIKS
PRWRGHAMEVGPLSRYILAYAHARSGNKYAERPKEQLEYSAQMINSAIPKALGLPETQYTLKQLLPSTIGRTLARALESQ
YCGEMMHSDWHDLVANIRAGDTATANVDKWDPATWPLQAKGVGTVAAPRGALGHWIRIKDGRIENYQCVVPTTWNGSPRD
YKGQIGAFEASLMNTPMVNPEQPVEILRTLHSFDPCLACSTHVMSAEGQELTTVKVR

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family

Homologues:

Organism=Escherichia coli, GI1787207, Length=619, Percent_Identity=57.3505654281099, Blast_Score=726, Evalue=0.0,
Organism=Escherichia coli, GI1789368, Length=607, Percent_Identity=43.32784184514, Blast_Score=473, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MBHL_CUPNH (P31891)

Other databases:

- EMBL:   M96433
- EMBL:   AY305378
- PIR:   B43255
- RefSeq:   NP_942644.1
- ProteinModelPortal:   P31891
- SMR:   P31891
- GeneID:   2656805
- GenomeReviews:   AY305378_GR
- KEGG:   reh:PHG002
- HOGENOM:   HBG733231
- OMA:   DGVTEPN
- PhylomeDB:   P31891
- ProtClustDB:   CLSK923789
- BioCyc:   MetaCyc:HOXGALCA-MONOMER
- BioCyc:   REUT381666:PHG002-MONOMER
- InterPro:   IPR001501
- InterPro:   IPR018194

Pfam domain/function: PF00374 NiFeSe_Hases

EC number: =1.12.99.6

Molecular weight: Translated: 68796; Mature: 68665

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00507 NI_HGENASE_L_1; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAYATQGFNLDDRGRRIVVDPVTRIEGHMRCEVNVDANNVIRNAVSTGTMWRGLEVILK
CCCCCCCCCCCCCCCCEEEECCHHHCCCCEEEEEECCHHHHHHHHHHHCHHHHCHHHEEE
GRDPRDAWAFVERICGVCTGCHALASVRAVENALDIRIPKNAHLIREIMAKTLQVHDHAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHEE
HFYHLHALDWVDVMSALKADPKRTSELQQLVSPAHPLSSAGYFRDIQNRLKRFVESGQLG
EEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
PFMNGYWGSKAYVLPPEANLMAVTHYLEALDLQKEWVKIHTIFGGKNPHPNYLVGGVPCA
CCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEECCCCEE
INLDGIGAASAPVNMERLSFVKARIDEIIEFNKNVYVPDVLAIGTLYKQAGWLYGGGLAA
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEECCCHHH
TNVLDYGEYPNVAYNKSTDQLPGGAILNGNWDEVFPVDPRDSQQVQEFVSHSWYKYADES
HHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
VGLHPWDGVTEPNYVLGANTKGTRTRIEQIDESAKYSWIKSPRWRGHAMEVGPLSRYILA
CCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHH
YAHARSGNKYAERPKEQLEYSAQMINSAIPKALGLPETQYTLKQLLPSTIGRTLARALES
HHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
QYCGEMMHSDWHDLVANIRAGDTATANVDKWDPATWPLQAKGVGTVAAPRGALGHWIRIK
HHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCEEEEE
DGRIENYQCVVPTTWNGSPRDYKGQIGAFEASLMNTPMVNPEQPVEILRTLHSFDPCLAC
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHH
STHVMSAEGQELTTVKVR
HHHHHCCCCCEEEEEEEC
>Mature Secondary Structure 
SAYATQGFNLDDRGRRIVVDPVTRIEGHMRCEVNVDANNVIRNAVSTGTMWRGLEVILK
CCCCCCCCCCCCCCCEEEECCHHHCCCCEEEEEECCHHHHHHHHHHHCHHHHCHHHEEE
GRDPRDAWAFVERICGVCTGCHALASVRAVENALDIRIPKNAHLIREIMAKTLQVHDHAV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHEE
HFYHLHALDWVDVMSALKADPKRTSELQQLVSPAHPLSSAGYFRDIQNRLKRFVESGQLG
EEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
PFMNGYWGSKAYVLPPEANLMAVTHYLEALDLQKEWVKIHTIFGGKNPHPNYLVGGVPCA
CCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEECCCCEE
INLDGIGAASAPVNMERLSFVKARIDEIIEFNKNVYVPDVLAIGTLYKQAGWLYGGGLAA
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEECCCHHH
TNVLDYGEYPNVAYNKSTDQLPGGAILNGNWDEVFPVDPRDSQQVQEFVSHSWYKYADES
HHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
VGLHPWDGVTEPNYVLGANTKGTRTRIEQIDESAKYSWIKSPRWRGHAMEVGPLSRYILA
CCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHH
YAHARSGNKYAERPKEQLEYSAQMINSAIPKALGLPETQYTLKQLLPSTIGRTLARALES
HHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
QYCGEMMHSDWHDLVANIRAGDTATANVDKWDPATWPLQAKGVGTVAAPRGALGHWIRIK
HHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCEEEEE
DGRIENYQCVVPTTWNGSPRDYKGQIGAFEASLMNTPMVNPEQPVEILRTLHSFDPCLAC
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHH
STHVMSAEGQELTTVKVR
HHHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1383192; 12948488; 2493816