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The map label for this gene is cca [H]

Identifier: 38234901

GI number: 38234901

Start: 2470765

End: 2472252

Strand: Reverse

Name: cca [H]

Synonym: DIP2368

Alternate gene names: 38234901

Gene position: 2472252-2470765 (Counterclockwise)

Preceding gene: 38234909

Following gene: 38234900

Centisome position: 99.34

GC content: 56.25

Gene sequence:

>1488_bases
ATGCGGGTGAATTCACAGAGTAACCATGATGTTTCCCCCGCCCCCTCCGGCGTGGTCTTAGAAGCTGCCGATCCAGCTGA
GCTGTTGGTCCGCGCACAGAAAACCCTGGGCTTACTCTCTCCGCTATTGAGCCCACTGGCTCAAGCGTTCCACGATCGTG
GGCACCGCCTCTACCTCGTAGGTGGTTCGGTGCGTGACGCCCTCCTTGATGAGCTTGGGCACGATCTGGATTGTACAACG
GATGCCCGCCCTGAGGTGGTATCCGCGATCCTGGAAGAGTACGCCCCCGTGGTCTGGGATACTGGTATCGAGTTTGGCAC
CGTTTCAGCAGAAAAAGACGGCCAACAAATCGAGATCACCACGTTCCGCTCCGACGTTTATGATGGGCAGTCCCGTAACC
CCGAGGTGCAATTCGGTGACACCCTTGAAGGTGATCTGATTCGCCGCGACTTCACCGTCAACGCTATGGCGATGGAGATC
CTGCCGGATGGCTCGATGCAGTTCCATGACCCCGTTGGTGGCATGAATGATCTAGTTGCGGGACTGTTAGACACCCCAGC
GACTCCGCAGCAAAGCTTTAACGACGACCCCCTGCGTATGCTGCGCGCTGCTCGTTTTGTTTCTCAGTTGGGCTTTGATC
TCGCACCACGAGTACGCCATGCCATGACGGAGATGGCGGATCAGATCACACGCATTACCGCTGAGCGCGTGCAGGTAGAG
CTGGACAAAATGATCTGTGGCAAAGTCCCATGGGATGGTATCGACGTGATGGTAGATACCGGATTAGCAGACATTATCTA
TCCGGAAATCTCTGCGCTGCGGCTTACTCAAGACGAACACCATCAACACAAGGATGTGTATGCCCACTCCTTGACGGTGT
TGCGCCAGGCGATGGATCAAGAAGAAGACGGCCCTGATGTGGTGTTGCGCTGGGCTGCCCTCATGCACGACTGTGGTAAG
CCAGATACACGCGAGTTCTCCCCCGAGGGGCGCGTGAGCTTCCACCAGCATGAGATCGTGGGTGCCCGCCTCATGCGTCA
TCGCATGCGCGCGTTGAAGTACTCCAAGCACACGATCCGTGACGTTAGTGAGCTCATTCGCCTGCACATGCGGTTCCATG
GATTTAGTGAGGGCGAGTGGACCGATTCGGCGGTTCGTCGCTACGTCACCGATGCCGGCGACCTACTCCCCCGCCTGCAC
AAGCTCGTGCGCGCCGACTGCACCACGCGAAACAAGAAGAAGGCGGAACGCCTACAAAAAACCTACGATCACTTAGAAGA
TCGCATTGCAGATATTGCTGCGAAGGAAGACCTAGCCAAGGTGCGCCCCGACCTAGACGGTAACGAGATCATGGAGATTC
TCGACATCAAGGCAGGACCCCAAGTGGGACAAGCATGGAAATTCTTAAAGGAACTGCGCTTAGAGCGTGGCCCCCTAGAA
CGTGAAGACGCGATTTTGGAATTGAAGAAATGGTGGTACGAACAGTAA

Upstream 100 bases:

>100_bases
ACGAACCACATTTGTGCCAAGATTTGCATAAGATTGTACCTCCAAGGAATAAGCCACTGGTTGCTTCATCGGGCTTTAGT
GCGTTGAAGAATGTAGACTC

Downstream 100 bases:

>100_bases
ATGTTTGCGGATCGACTCTTTAACGCCATGGAGCGCAATGAACCCGCGCCGGGCATGGTGCTAGTTGCTGCACCTTCGAT
GGAATCGGAGGATTTTGCGC

Product: putative tRNA nucleotidyltransferase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MRVNSQSNHDVSPAPSGVVLEAADPAELLVRAQKTLGLLSPLLSPLAQAFHDRGHRLYLVGGSVRDALLDELGHDLDCTT
DARPEVVSAILEEYAPVVWDTGIEFGTVSAEKDGQQIEITTFRSDVYDGQSRNPEVQFGDTLEGDLIRRDFTVNAMAMEI
LPDGSMQFHDPVGGMNDLVAGLLDTPATPQQSFNDDPLRMLRAARFVSQLGFDLAPRVRHAMTEMADQITRITAERVQVE
LDKMICGKVPWDGIDVMVDTGLADIIYPEISALRLTQDEHHQHKDVYAHSLTVLRQAMDQEEDGPDVVLRWAALMHDCGK
PDTREFSPEGRVSFHQHEIVGARLMRHRMRALKYSKHTIRDVSELIRLHMRFHGFSEGEWTDSAVRRYVTDAGDLLPRLH
KLVRADCTTRNKKKAERLQKTYDHLEDRIADIAAKEDLAKVRPDLDGNEIMEILDIKAGPQVGQAWKFLKELRLERGPLE
REDAILELKKWWYEQ

Sequences:

>Translated_495_residues
MRVNSQSNHDVSPAPSGVVLEAADPAELLVRAQKTLGLLSPLLSPLAQAFHDRGHRLYLVGGSVRDALLDELGHDLDCTT
DARPEVVSAILEEYAPVVWDTGIEFGTVSAEKDGQQIEITTFRSDVYDGQSRNPEVQFGDTLEGDLIRRDFTVNAMAMEI
LPDGSMQFHDPVGGMNDLVAGLLDTPATPQQSFNDDPLRMLRAARFVSQLGFDLAPRVRHAMTEMADQITRITAERVQVE
LDKMICGKVPWDGIDVMVDTGLADIIYPEISALRLTQDEHHQHKDVYAHSLTVLRQAMDQEEDGPDVVLRWAALMHDCGK
PDTREFSPEGRVSFHQHEIVGARLMRHRMRALKYSKHTIRDVSELIRLHMRFHGFSEGEWTDSAVRRYVTDAGDLLPRLH
KLVRADCTTRNKKKAERLQKTYDHLEDRIADIAAKEDLAKVRPDLDGNEIMEILDIKAGPQVGQAWKFLKELRLERGPLE
REDAILELKKWWYEQ
>Mature_495_residues
MRVNSQSNHDVSPAPSGVVLEAADPAELLVRAQKTLGLLSPLLSPLAQAFHDRGHRLYLVGGSVRDALLDELGHDLDCTT
DARPEVVSAILEEYAPVVWDTGIEFGTVSAEKDGQQIEITTFRSDVYDGQSRNPEVQFGDTLEGDLIRRDFTVNAMAMEI
LPDGSMQFHDPVGGMNDLVAGLLDTPATPQQSFNDDPLRMLRAARFVSQLGFDLAPRVRHAMTEMADQITRITAERVQVE
LDKMICGKVPWDGIDVMVDTGLADIIYPEISALRLTQDEHHQHKDVYAHSLTVLRQAMDQEEDGPDVVLRWAALMHDCGK
PDTREFSPEGRVSFHQHEIVGARLMRHRMRALKYSKHTIRDVSELIRLHMRFHGFSEGEWTDSAVRRYVTDAGDLLPRLH
KLVRADCTTRNKKKAERLQKTYDHLEDRIADIAAKEDLAKVRPDLDGNEIMEILDIKAGPQVGQAWKFLKELRLERGPLE
REDAILELKKWWYEQ

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=235, Percent_Identity=30.6382978723404, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1789436, Length=334, Percent_Identity=31.1377245508982, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI87081691, Length=229, Percent_Identity=28.82096069869, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71995920, Length=246, Percent_Identity=27.6422764227642, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321016, Length=529, Percent_Identity=24.7637051039698, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI281360112, Length=349, Percent_Identity=25.214899713467, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21357337, Length=349, Percent_Identity=25.214899713467, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 55998; Mature: 55998

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVNSQSNHDVSPAPSGVVLEAADPAELLVRAQKTLGLLSPLLSPLAQAFHDRGHRLYLV
CCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GGSVRDALLDELGHDLDCTTDARPEVVSAILEEYAPVVWDTGIEFGTVSAEKDGQQIEIT
CCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEE
TFRSDVYDGQSRNPEVQFGDTLEGDLIRRDFTVNAMAMEILPDGSMQFHDPVGGMNDLVA
EEHHHHCCCCCCCCCCEECCCCCCCHHHCCCCHHHEEEEECCCCCCCCCCCCCCHHHHHH
GLLDTPATPQQSFNDDPLRMLRAARFVSQLGFDLAPRVRHAMTEMADQITRITAERVQVE
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDKMICGKVPWDGIDVMVDTGLADIIYPEISALRLTQDEHHQHKDVYAHSLTVLRQAMDQ
HHHHHHCCCCCCCCHHEECCCCHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
EEDGPDVVLRWAALMHDCGKPDTREFSPEGRVSFHQHEIVGARLMRHRMRALKYSKHTIR
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVSELIRLHMRFHGFSEGEWTDSAVRRYVTDAGDLLPRLHKLVRADCTTRNKKKAERLQK
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TYDHLEDRIADIAAKEDLAKVRPDLDGNEIMEILDIKAGPQVGQAWKFLKELRLERGPLE
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
REDAILELKKWWYEQ
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVNSQSNHDVSPAPSGVVLEAADPAELLVRAQKTLGLLSPLLSPLAQAFHDRGHRLYLV
CCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GGSVRDALLDELGHDLDCTTDARPEVVSAILEEYAPVVWDTGIEFGTVSAEKDGQQIEIT
CCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEE
TFRSDVYDGQSRNPEVQFGDTLEGDLIRRDFTVNAMAMEILPDGSMQFHDPVGGMNDLVA
EEHHHHCCCCCCCCCCEECCCCCCCHHHCCCCHHHEEEEECCCCCCCCCCCCCCHHHHHH
GLLDTPATPQQSFNDDPLRMLRAARFVSQLGFDLAPRVRHAMTEMADQITRITAERVQVE
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDKMICGKVPWDGIDVMVDTGLADIIYPEISALRLTQDEHHQHKDVYAHSLTVLRQAMDQ
HHHHHHCCCCCCCCHHEECCCCHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
EEDGPDVVLRWAALMHDCGKPDTREFSPEGRVSFHQHEIVGARLMRHRMRALKYSKHTIR
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVSELIRLHMRFHGFSEGEWTDSAVRRYVTDAGDLLPRLHKLVRADCTTRNKKKAERLQK
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TYDHLEDRIADIAAKEDLAKVRPDLDGNEIMEILDIKAGPQVGQAWKFLKELRLERGPLE
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
REDAILELKKWWYEQ
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA