Click here to switch to the map view.

The map label for this gene is ldh

Identifier: 38234791

GI number: 38234791

Start: 2347413

End: 2348369

Strand: Reverse

Name: ldh

Synonym: DIP2255

Alternate gene names: 38234791

Gene position: 2348369-2347413 (Counterclockwise)

Preceding gene: 38234792

Following gene: 38234790

Centisome position: 94.36

GC content: 57.68

Gene sequence:

>957_bases
GTGAAGAACATCGTCGGAAACAAGGTGGTTCTCATCGGAGCAGGCGACGTGGGAGTGGCCTACGCATACGCACTGGTCAA
CCAGGGTACAGTAGACCACCTCGCCATCATCGACATCGATGAGAAAAAACTCGAGGGCAACGTGATGGATCTCAACCACG
GTGTGGTGTGGGCGTCGTCACGCACGAAGGTTTCTAAGGGCACCTACGCCGACTGTGAAGACGCAGCCGTCGTAGTCATC
TGCGCAGGCGCGGCACAAAAGCCAGGCGAAACCCGCCTCCAGCTCGTCGACAAGAACATGAAGATCATGAAGTCCATCGT
GGACAACGTCATGGCCAACAACTTCGACGGCATCTTCCTCGTAGCCTCCAACCCAGTCGACATCCTCACCTACGCCGTAT
GGAAGTACTCCGGATTCGACCACCACCGCGTCATTGGCTCCGGCACCGTCCTCGACTCCGCACGCTTCCGCTACATGCTC
GGCGAGCGTTACGACGTCGCCCCAAGCTCCATCCACGCCTACATCATCGGCGAACACGGCGACACCGAACTCCCAGTCCT
CTCCTCTGCAACCGTCGCCGGCGTATCCATGCGCCGCCAACTGCAAAAGAACCCAGGGCTCGAAGAAGAACTCGAGAAAA
TCTTCGAAGAAACCCGCGACGCCGCCTACACCATCATCGACGCCAAAGGCTCCACCTCCTACGGCATTGGCATGGGTCTA
GCGCGCATTACTCGCGCCATCATCCACAACCAAGAAGTGGCACTGCCTGTCTCCGCCTATCTGGAAGGACAGTACGGCCA
GGAAGACATCTACATTGGTACTCCAGCCGTGATCAACCGTGCTGGTATCAACCGCGTTGTGGAACTCGAGCTCACCGCCC
ACGAAATGGAACGCTTCATCCACTCCGCAAATACCCTCCGCGAAGTCCAAGAGAAGTTCTTCCCACCAGCTAACTAG

Upstream 100 bases:

>100_bases
AATGGGGTGTTGGGTCCCGCTGTGCACGATACTGCCACACCGCTGCACACTAGGCACACTAGAGGCTCACGAGCCGATCA
CGAATTTTAGAAAGGTCATT

Downstream 100 bases:

>100_bases
GGCGCTCATTTTTGGTAAGTGAGTAGTTCGTGCCAAAACTTTTCTACGTCCGCGGCGTTTCCGCAGGTCGGAGATATTAG
AAAAGATTTAATTTGGCACG

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKNIVGNKVVLIGAGDVGVAYAYALVNQGTVDHLAIIDIDEKKLEGNVMDLNHGVVWASSRTKVSKGTYADCEDAAVVVI
CAGAAQKPGETRLQLVDKNMKIMKSIVDNVMANNFDGIFLVASNPVDILTYAVWKYSGFDHHRVIGSGTVLDSARFRYML
GERYDVAPSSIHAYIIGEHGDTELPVLSSATVAGVSMRRQLQKNPGLEEELEKIFEETRDAAYTIIDAKGSTSYGIGMGL
ARITRAIIHNQEVALPVSAYLEGQYGQEDIYIGTPAVINRAGINRVVELELTAHEMERFIHSANTLREVQEKFFPPAN

Sequences:

>Translated_318_residues
MKNIVGNKVVLIGAGDVGVAYAYALVNQGTVDHLAIIDIDEKKLEGNVMDLNHGVVWASSRTKVSKGTYADCEDAAVVVI
CAGAAQKPGETRLQLVDKNMKIMKSIVDNVMANNFDGIFLVASNPVDILTYAVWKYSGFDHHRVIGSGTVLDSARFRYML
GERYDVAPSSIHAYIIGEHGDTELPVLSSATVAGVSMRRQLQKNPGLEEELEKIFEETRDAAYTIIDAKGSTSYGIGMGL
ARITRAIIHNQEVALPVSAYLEGQYGQEDIYIGTPAVINRAGINRVVELELTAHEMERFIHSANTLREVQEKFFPPAN
>Mature_318_residues
MKNIVGNKVVLIGAGDVGVAYAYALVNQGTVDHLAIIDIDEKKLEGNVMDLNHGVVWASSRTKVSKGTYADCEDAAVVVI
CAGAAQKPGETRLQLVDKNMKIMKSIVDNVMANNFDGIFLVASNPVDILTYAVWKYSGFDHHRVIGSGTVLDSARFRYML
GERYDVAPSSIHAYIIGEHGDTELPVLSSATVAGVSMRRQLQKNPGLEEELEKIFEETRDAAYTIIDAKGSTSYGIGMGL
ARITRAIIHNQEVALPVSAYLEGQYGQEDIYIGTPAVINRAGINRVVELELTAHEMERFIHSANTLREVQEKFFPPAN

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI5031857, Length=311, Percent_Identity=40.5144694533762, Blast_Score=248, Evalue=4e-66,
Organism=Homo sapiens, GI260099723, Length=311, Percent_Identity=40.5144694533762, Blast_Score=248, Evalue=5e-66,
Organism=Homo sapiens, GI9257228, Length=313, Percent_Identity=40.5750798722045, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI4504973, Length=313, Percent_Identity=40.5750798722045, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI47059044, Length=311, Percent_Identity=39.871382636656, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI221136809, Length=311, Percent_Identity=39.871382636656, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI291575128, Length=313, Percent_Identity=37.3801916932907, Blast_Score=235, Evalue=3e-62,
Organism=Homo sapiens, GI4557032, Length=313, Percent_Identity=37.3801916932907, Blast_Score=235, Evalue=3e-62,
Organism=Homo sapiens, GI15082234, Length=311, Percent_Identity=38.2636655948553, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI260099725, Length=225, Percent_Identity=44.4444444444444, Blast_Score=194, Evalue=8e-50,
Organism=Homo sapiens, GI260099727, Length=210, Percent_Identity=45.2380952380952, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI207028494, Length=197, Percent_Identity=41.1167512690355, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI103472011, Length=316, Percent_Identity=25.9493670886076, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI103472015, Length=189, Percent_Identity=30.1587301587302, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI21735621, Length=308, Percent_Identity=24.6753246753247, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789632, Length=318, Percent_Identity=28.9308176100629, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17535107, Length=297, Percent_Identity=39.7306397306397, Blast_Score=226, Evalue=9e-60,
Organism=Caenorhabditis elegans, GI17554310, Length=309, Percent_Identity=27.5080906148867, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17136226, Length=311, Percent_Identity=39.5498392282958, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI45550422, Length=311, Percent_Identity=30.5466237942122, Blast_Score=161, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24647881, Length=292, Percent_Identity=28.0821917808219, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24663599, Length=274, Percent_Identity=26.6423357664234, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH_CORDI (P62050)

Other databases:

- EMBL:   BX248360
- RefSeq:   NP_940558.1
- ProteinModelPortal:   P62050
- SMR:   P62050
- GeneID:   2650051
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP2255
- NMPDR:   fig|257309.1.peg.2149
- HOGENOM:   HBG566126
- OMA:   MDLNHGV
- ProtClustDB:   PRK00066
- BioCyc:   CDIP257309:DIP2255-MONOMER
- BRENDA:   1.1.1.27
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 34757; Mature: 34757

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00064 L_LDH

Important sites: ACT_SITE 179-179 BINDING 92-92 BINDING 124-124 BINDING 155-155 BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIVGNKVVLIGAGDVGVAYAYALVNQGTVDHLAIIDIDEKKLEGNVMDLNHGVVWASS
CCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCEEEECCCEEEECC
RTKVSKGTYADCEDAAVVVICAGAAQKPGETRLQLVDKNMKIMKSIVDNVMANNFDGIFL
CCEECCCCCCCCCCCEEEEEECCCCCCCCCHHHEEHHCHHHHHHHHHHHHHHCCCCCEEE
VASNPVDILTYAVWKYSGFDHHRVIGSGTVLDSARFRYMLGERYDVAPSSIHAYIIGEHG
EECCCHHHHHHHHHHCCCCCCEEEEECCCEECCHHHHHHHCCCCCCCCCCEEEEEEECCC
DTELPVLSSATVAGVSMRRQLQKNPGLEEELEKIFEETRDAAYTIIDAKGSTSYGIGMGL
CCCCCEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHH
ARITRAIIHNQEVALPVSAYLEGQYGQEDIYIGTPAVINRAGINRVVELELTAHEMERFI
HHHHHHHHCCCCEEEEHHHEECCCCCCCCEEECCHHHHHHCCCCEEEEEEECHHHHHHHH
HSANTLREVQEKFFPPAN
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKNIVGNKVVLIGAGDVGVAYAYALVNQGTVDHLAIIDIDEKKLEGNVMDLNHGVVWASS
CCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCEEEEEECCHHHHCCCEEEECCCEEEECC
RTKVSKGTYADCEDAAVVVICAGAAQKPGETRLQLVDKNMKIMKSIVDNVMANNFDGIFL
CCEECCCCCCCCCCCEEEEEECCCCCCCCCHHHEEHHCHHHHHHHHHHHHHHCCCCCEEE
VASNPVDILTYAVWKYSGFDHHRVIGSGTVLDSARFRYMLGERYDVAPSSIHAYIIGEHG
EECCCHHHHHHHHHHCCCCCCEEEEECCCEECCHHHHHHHCCCCCCCCCCEEEEEEECCC
DTELPVLSSATVAGVSMRRQLQKNPGLEEELEKIFEETRDAAYTIIDAKGSTSYGIGMGL
CCCCCEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHH
ARITRAIIHNQEVALPVSAYLEGQYGQEDIYIGTPAVINRAGINRVVELELTAHEMERFI
HHHHHHHHCCCCEEEEHHHEECCCCCCCCEEECCHHHHHHCCCCEEEEEEECHHHHHHHH
HSANTLREVQEKFFPPAN
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910