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The map label for this gene is tig

Identifier: 38234364

GI number: 38234364

Start: 1840874

End: 1842220

Strand: Reverse

Name: tig

Synonym: DIP1793

Alternate gene names: 38234364

Gene position: 1842220-1840874 (Counterclockwise)

Preceding gene: 38234367

Following gene: 38234363

Centisome position: 74.03

GC content: 57.02

Gene sequence:

>1347_bases
GTGAAGAGTTCCGTCGAAAAGCTGAGCGAGACCCGCGTTAAGCTCAATGTAGAGGTTCCTTTCGAGGAGCTAAAGCCAGA
GATCGACCAGGCTTACAAGGCTTTGGCTCAGCAGATCACCATCCCTGGCTTCCGTCGCGGTAAGGCCCCACGCCAGCTTA
TCGACGCCCGCATCGGTCGCGGCGCAGTCCTCGAGCAGGTCATTAACGATATGCTGCCTACCCGCTACCAGCAGGTTGTG
GAAGAAAACGAGCTTGTCGTTCTCGGCCAGCCACACATTGACATCACCAAGCTGGAAGACAACGAGGTTGTCGAGTTTAC
CGCTGAGGTTGATGTTCGCCCAGAGATCACCGTTCCTGATTTCTCCGCTTTCGCGGTTGAGGTTCCTGCTCTTAAGTCTA
ATGACGAGGCAATCGATGCGCAGATCGATAAGTTGCGTGAGCGTTTCGGTGAGCTGAAGGACACCAAGCGCAAGCTGAAG
ACTGACGACTTTGCCATCATTGATATCGAGGCTGCTATCGATGGTGAGAAGCTGGAAGAGGCTACCACTGAGGGTATGTC
CTACCAGGTTGGCGCTGGCGACCTCATCGATGGTCTGGACACCGCACTGCGTGGTCTTAAGGCCGGTGAGTCCGCTGAGT
TCACCACCACCTTGAAGGCTGGCGAGCATGAGGGCAAGGAAGCTGCTGTTACCGTAACTGTTCAGCAGACCAAGGAGCGC
AAGCTTCCTGAGCTCGACGAAGAGTTCGTTCAGACCGCTTCTGAGTTCGACACCGTCGAAGAGCTGCGCGAATCCATCGC
AGAGCAGGTTGCTGAGCAGGCAAAGGCTGAGCAGGCTACCGCAATCCGCGACGAAGTTCTCAAGGCTGCCCTCGCAGAGG
CAACCTTCGAGCTGCCAGAAGGCGTTGTCGAGGATCAGATCCACGCACAGCTGCACCAGCTCCTCAACCAGGTTGGCGGC
GACGAGGCTGCCCTCAACGCTGCACTCGAGGCTCAGGGTACTTCCCGCGAGCAGTTCGATGCTGACAACCGCAAGAACTC
CGAGGAAGCTGTTCGTACCCAGCTGTTCTTGGACGCTTTGGCAGAGCAGGAGCAGCCAGAGGTTTCTCAGCAGGAGCTGA
CCGACCACATTCTGTTCACCGCTCAGTCCTACGGTATGGACCCTAACCAGTTCATCACCCAGCTGCAGCAGTCCGGTCAG
ATCGGCAACCTGTTCTCCGACGTTCGTCGCGGCAAGGCACTGGCAGCAGCTATCTGCCGTGTTTCCGTGAAGGACGACGA
GGGCAAAGCTGTTGACCCAAGCGAGTACTTCGGTGAGGAAGAAGAGTCCGCAGAGGAGTCTGAGTAA

Upstream 100 bases:

>100_bases
AAATCCTGTCGTCCCGACAAAAATCCCTCCACTTGAATATAGGTGGAGGGATTTTTCTAATTCAACTATCCCCTATAAAA
AACGACCAGGAGAGTTACTC

Downstream 100 bases:

>100_bases
TTTCTTCATTGTGAAAGGCCCAGCCTCGGCTGGGCCTTTTGCGTACACTGCAGGGCTTCGACGCTGAGAGCGAACAGAGA
AGCTATGTGGAACAAACGGC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MKSSVEKLSETRVKLNVEVPFEELKPEIDQAYKALAQQITIPGFRRGKAPRQLIDARIGRGAVLEQVINDMLPTRYQQVV
EENELVVLGQPHIDITKLEDNEVVEFTAEVDVRPEITVPDFSAFAVEVPALKSNDEAIDAQIDKLRERFGELKDTKRKLK
TDDFAIIDIEAAIDGEKLEEATTEGMSYQVGAGDLIDGLDTALRGLKAGESAEFTTTLKAGEHEGKEAAVTVTVQQTKER
KLPELDEEFVQTASEFDTVEELRESIAEQVAEQAKAEQATAIRDEVLKAALAEATFELPEGVVEDQIHAQLHQLLNQVGG
DEAALNAALEAQGTSREQFDADNRKNSEEAVRTQLFLDALAEQEQPEVSQQELTDHILFTAQSYGMDPNQFITQLQQSGQ
IGNLFSDVRRGKALAAAICRVSVKDDEGKAVDPSEYFGEEEESAEESE

Sequences:

>Translated_448_residues
MKSSVEKLSETRVKLNVEVPFEELKPEIDQAYKALAQQITIPGFRRGKAPRQLIDARIGRGAVLEQVINDMLPTRYQQVV
EENELVVLGQPHIDITKLEDNEVVEFTAEVDVRPEITVPDFSAFAVEVPALKSNDEAIDAQIDKLRERFGELKDTKRKLK
TDDFAIIDIEAAIDGEKLEEATTEGMSYQVGAGDLIDGLDTALRGLKAGESAEFTTTLKAGEHEGKEAAVTVTVQQTKER
KLPELDEEFVQTASEFDTVEELRESIAEQVAEQAKAEQATAIRDEVLKAALAEATFELPEGVVEDQIHAQLHQLLNQVGG
DEAALNAALEAQGTSREQFDADNRKNSEEAVRTQLFLDALAEQEQPEVSQQELTDHILFTAQSYGMDPNQFITQLQQSGQ
IGNLFSDVRRGKALAAAICRVSVKDDEGKAVDPSEYFGEEEESAEESE
>Mature_448_residues
MKSSVEKLSETRVKLNVEVPFEELKPEIDQAYKALAQQITIPGFRRGKAPRQLIDARIGRGAVLEQVINDMLPTRYQQVV
EENELVVLGQPHIDITKLEDNEVVEFTAEVDVRPEITVPDFSAFAVEVPALKSNDEAIDAQIDKLRERFGELKDTKRKLK
TDDFAIIDIEAAIDGEKLEEATTEGMSYQVGAGDLIDGLDTALRGLKAGESAEFTTTLKAGEHEGKEAAVTVTVQQTKER
KLPELDEEFVQTASEFDTVEELRESIAEQVAEQAKAEQATAIRDEVLKAALAEATFELPEGVVEDQIHAQLHQLLNQVGG
DEAALNAALEAQGTSREQFDADNRKNSEEAVRTQLFLDALAEQEQPEVSQQELTDHILFTAQSYGMDPNQFITQLQQSGQ
IGNLFSDVRRGKALAAAICRVSVKDDEGKAVDPSEYFGEEEESAEESE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=327, Percent_Identity=22.9357798165138, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_CORDI (Q6NFU3)

Other databases:

- EMBL:   BX248359
- RefSeq:   NP_940131.1
- ProteinModelPortal:   Q6NFU3
- GeneID:   2649418
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1793
- NMPDR:   fig|257309.1.peg.1722
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   CDIP257309:DIP1793-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 49515; Mature: 49515

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSVEKLSETRVKLNVEVPFEELKPEIDQAYKALAQQITIPGFRRGKAPRQLIDARIGR
CCCHHHHHHHCEEEEEEECCHHHHCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
GAVLEQVINDMLPTRYQQVVEENELVVLGQPHIDITKLEDNEVVEFTAEVDVRPEITVPD
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCEEEEEEEECCCCCCCCCC
FSAFAVEVPALKSNDEAIDAQIDKLRERFGELKDTKRKLKTDDFAIIDIEAAIDGEKLEE
CCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHH
ATTEGMSYQVGAGDLIDGLDTALRGLKAGESAEFTTTLKAGEHEGKEAAVTVTVQQTKER
HHHCCCEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEEEECHHHC
KLPELDEEFVQTASEFDTVEELRESIAEQVAEQAKAEQATAIRDEVLKAALAEATFELPE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GVVEDQIHAQLHQLLNQVGGDEAALNAALEAQGTSREQFDADNRKNSEEAVRTQLFLDAL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHH
AEQEQPEVSQQELTDHILFTAQSYGMDPNQFITQLQQSGQIGNLFSDVRRGKALAAAICR
HHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VSVKDDEGKAVDPSEYFGEEEESAEESE
EEEECCCCCCCCCHHHCCCCHHCCCCCC
>Mature Secondary Structure
MKSSVEKLSETRVKLNVEVPFEELKPEIDQAYKALAQQITIPGFRRGKAPRQLIDARIGR
CCCHHHHHHHCEEEEEEECCHHHHCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
GAVLEQVINDMLPTRYQQVVEENELVVLGQPHIDITKLEDNEVVEFTAEVDVRPEITVPD
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCEEEEEEEECCCCCCCCCC
FSAFAVEVPALKSNDEAIDAQIDKLRERFGELKDTKRKLKTDDFAIIDIEAAIDGEKLEE
CCHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHH
ATTEGMSYQVGAGDLIDGLDTALRGLKAGESAEFTTTLKAGEHEGKEAAVTVTVQQTKER
HHHCCCEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEEEECHHHC
KLPELDEEFVQTASEFDTVEELRESIAEQVAEQAKAEQATAIRDEVLKAALAEATFELPE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GVVEDQIHAQLHQLLNQVGGDEAALNAALEAQGTSREQFDADNRKNSEEAVRTQLFLDAL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHCCCCCCCCHHHHHHHHHHHHHH
AEQEQPEVSQQELTDHILFTAQSYGMDPNQFITQLQQSGQIGNLFSDVRRGKALAAAICR
HHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
VSVKDDEGKAVDPSEYFGEEEESAEESE
EEEECCCCCCCCCHHHCCCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910