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The map label for this gene is rsmE [H]

Identifier: 38234290

GI number: 38234290

Start: 1755611

End: 1756369

Strand: Reverse

Name: rsmE [H]

Synonym: DIP1719

Alternate gene names: 38234290

Gene position: 1756369-1755611 (Counterclockwise)

Preceding gene: 38234291

Following gene: 38234289

Centisome position: 70.58

GC content: 57.31

Gene sequence:

>759_bases
ATGTCGCTTCCAGTTTTTATCTATAACCTCGAAGACCTCGTCGCCGGTAAGCACAGTGAGATTCCTACCGGCAATGTTGT
CCTCGATGGGGCAGAAGGACGCCACGCGGTCAGCGTTAAACGGATGACAGCAGGCGAGCAGATCAAACTGATCGACGGCC
GAGGCGGTTGGGCAACAGCGACAGTGGCGTCGACAAGCGGGAAAAGTACGCTAAATCTTCAAGTTTTCGAATGCAATGAA
GAAGCCACCCCCACACCTACGGTGACTGTTTTCCAAGCGATTCCGAAATCGGAACGCTCAGAATTGGCCGTTGATCTGCT
TACCCAAGGTGGCGCCGATGCGATCGTTCCGTGGGAAGCGCATCGATGCGTAGCCAAGTGGGTCGGCGCCAAACGATCCA
AGGGCGTGCTCAAATGGCAGCAAGCAGCAATCGCCGCTGCGAAGCAGTCGCGACGCACTCGAATCCCCGAGATTGCCGAG
CCAATGACCACCGCTCAGATCGCAGAACAACTCGAGCAATTCGACGTTGTGCTGGTGCTTCACGAGGACGCCGCAGTGCC
GTTCGCTACGGTTGATGTCAGCACCGCAAAGTCAATTGCCTTAATCATTGGCCCCGAAGGTGGAGTGAGCCCCGACGAAG
CAGAAGCATTCCGTAAAGCTGGTGCCCGCGCCGTAAAGCTCGGCCCCGAGGTACTGCGGACTGCATCAGCGGGAATCGTG
GCGCTGGCTGCTCTCGGAGTACAGACTTCTCGCTGGTAG

Upstream 100 bases:

>100_bases
AAGCTTCGGGATCACCGTAGCGATGAATCGTCAGTCCGGACTGCTGACGATTCTGATGATTCCCTCTTCGGTCGCCTCCG
CAATAGGTTCCGTCGCTAAC

Downstream 100 bases:

>100_bases
TGTGGCAAACAACGAACATTTTCTCTCAGATAGGTTGAACCGAAACGTTGGCTGACTTTATAACTCGCGCCATTGAACTG
GACGAGCAATACTCTCAAGC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MSLPVFIYNLEDLVAGKHSEIPTGNVVLDGAEGRHAVSVKRMTAGEQIKLIDGRGGWATATVASTSGKSTLNLQVFECNE
EATPTPTVTVFQAIPKSERSELAVDLLTQGGADAIVPWEAHRCVAKWVGAKRSKGVLKWQQAAIAAAKQSRRTRIPEIAE
PMTTAQIAEQLEQFDVVLVLHEDAAVPFATVDVSTAKSIALIIGPEGGVSPDEAEAFRKAGARAVKLGPEVLRTASAGIV
ALAALGVQTSRW

Sequences:

>Translated_252_residues
MSLPVFIYNLEDLVAGKHSEIPTGNVVLDGAEGRHAVSVKRMTAGEQIKLIDGRGGWATATVASTSGKSTLNLQVFECNE
EATPTPTVTVFQAIPKSERSELAVDLLTQGGADAIVPWEAHRCVAKWVGAKRSKGVLKWQQAAIAAAKQSRRTRIPEIAE
PMTTAQIAEQLEQFDVVLVLHEDAAVPFATVDVSTAKSIALIIGPEGGVSPDEAEAFRKAGARAVKLGPEVLRTASAGIV
ALAALGVQTSRW
>Mature_251_residues
SLPVFIYNLEDLVAGKHSEIPTGNVVLDGAEGRHAVSVKRMTAGEQIKLIDGRGGWATATVASTSGKSTLNLQVFECNEE
ATPTPTVTVFQAIPKSERSELAVDLLTQGGADAIVPWEAHRCVAKWVGAKRSKGVLKWQQAAIAAAKQSRRTRIPEIAEP
MTTAQIAEQLEQFDVVLVLHEDAAVPFATVDVSTAKSIALIIGPEGGVSPDEAEAFRKAGARAVKLGPEVLRTASAGIVA
LAALGVQTSRW

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=244, Percent_Identity=31.1475409836066, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26641; Mature: 26509

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPVFIYNLEDLVAGKHSEIPTGNVVLDGAEGRHAVSVKRMTAGEQIKLIDGRGGWATA
CCCCEEEEEHHHHHCCCCCCCCCCCEEEECCCCCEEEEEEEECCCCEEEEEECCCCEEEE
TVASTSGKSTLNLQVFECNEEATPTPTVTVFQAIPKSERSELAVDLLTQGGADAIVPWEA
EEEECCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEECCCHH
HRCVAKWVGAKRSKGVLKWQQAAIAAAKQSRRTRIPEIAEPMTTAQIAEQLEQFDVVLVL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHCCEEEEE
HEDAAVPFATVDVSTAKSIALIIGPEGGVSPDEAEAFRKAGARAVKLGPEVLRTASAGIV
ECCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHCCCHH
ALAALGVQTSRW
HHHHHCCCCCCC
>Mature Secondary Structure 
SLPVFIYNLEDLVAGKHSEIPTGNVVLDGAEGRHAVSVKRMTAGEQIKLIDGRGGWATA
CCCEEEEEHHHHHCCCCCCCCCCCEEEECCCCCEEEEEEEECCCCEEEEEECCCCEEEE
TVASTSGKSTLNLQVFECNEEATPTPTVTVFQAIPKSERSELAVDLLTQGGADAIVPWEA
EEEECCCCCEEEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCEECCCHH
HRCVAKWVGAKRSKGVLKWQQAAIAAAKQSRRTRIPEIAEPMTTAQIAEQLEQFDVVLVL
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCHHHHHHHHHHHHHCCEEEEE
HEDAAVPFATVDVSTAKSIALIIGPEGGVSPDEAEAFRKAGARAVKLGPEVLRTASAGIV
ECCCCCCEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHCCCEEEECCHHHHHHHCCCHH
ALAALGVQTSRW
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]