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The map label for this gene is dnaG [H]

Identifier: 38234270

GI number: 38234270

Start: 1736085

End: 1737983

Strand: Reverse

Name: dnaG [H]

Synonym: DIP1699

Alternate gene names: 38234270

Gene position: 1737983-1736085 (Counterclockwise)

Preceding gene: 38234271

Following gene: 38234267

Centisome position: 69.84

GC content: 53.29

Gene sequence:

>1899_bases
ATGGCAGGCAAGGGTCGAATACCAGATAGTGACATTCAGGCGATTCGTGAGCGAACCCCCATCGAGGAAATCGTAGGGGA
ATATGTTCAGCTCAAGCCTGCCGGAGCGGACTCGTTGAAAGGTCTGAGTCCGTTCAAAGAAGAGAAAACGCCGTCATTTC
ATGTGCGGCCCAACCATGGATACTTCCACTGCTTTTCCACCGGAAAAGGCGGCGACGTTTTCTCCTTCCTCATGGAGATG
GAACAGCTAACTTTCCCAGAGGCTGTAGAAGTCTGTGCCGAAAAAATCGGCTACCAGATCAATTATCAGGGTGGAACCAC
CGGGGCTCGACGCGAAGAACCAGGAACACGCAAACGCCTTATTGAGGCAAACCGTGTAGCCCACCAGTTCTATCGCCAAC
AATTAGAAACCCCAGAGGCAGCAGTTGCTCGCCAATTCCTCTTGGACAGGGGATTTTCACAGCAGCATATCTATGGCTTT
GAGTGCGGTTATGCACCCGAAGGCTGGGATACCATGACCAAATTTATGCTGCGTAAGGGCTTTGAATTCAAAGAACTTGA
AGCAGCAGGCCTATCCACTATGGGGCGACGCGGCCCAATCGACCGCTTCCATCGTCGATTGCTCTGGCCGATTAAGTCGG
TGTCCGGTGATGTGATTGGTTTCGGTGCTCGCAAGCTCTTTGACGACGACAAACTCGGCAAGTACATGAACACCCCCGAG
ACGTTGTTGTACAAAAAGTCGAAAGTGCTCTTTGGCCTAGATCTGGCGAAAAAATCTATCGCTAGCCAACACCAAGCTGT
GGTCGTGGAAGGCTACACAGATGTGATGGCCATGCATGCAGCGGGAATCACTACTGCGGTGGCAGCCTGTGGTACAGCCT
TCGGTGAAGAACACCTACAAATCCTGCGTCGATACATGCTTGACGACGCCTACTTCGCTGGATCCCTTATCTACACCTTC
GATGGTGATGAGGCCGGACAAAAAGCTGCTATGCGTGCCTTCGAGGGCGACCAAAAATTCAGTGGACAGTCCTATGTGGC
TGTTGCCCCTGACGGCATGGATCCATGCGATCTGCGCCTAGCAAAAGGTGATGCAGCGGTGCGTGATCTCGTAGCACAGC
GAGTCCCCATGTTCGAATTCATCATTCGAACTTTGATTTCGGAATATCCCTTGGACTCAGTGGAAGGGCGACTCCAAGCG
TTGCGACGTGCCGTGCCCATTGTGGCGTCGATACGCGACCGTGCTCTAAAAACTGAATATGCCAGATTGCTTGCCGGCTG
GGTGGGTTGGCCTGATCCTAACGAGGTTTTGCAACAAGTCCGAGCCGAAGAACGCCGACCTAAAACCCAGCCTCGCCAGC
GCAGGGCAACCCGTATCGACTCTAATAGTCAGCCAGCGATGATTCAGTCCATGTTGGTCATGCCGGACCGAAAAGAGCCA
CGGCTATGGCCGCAGCGAGAGGCCCTCAAAATCGCGCTACAATACCCTGAACACGCAGGGGAGTACTTTGATAGCCTCCC
CGACGATGCGTTTACCCACGAGTCCTATGCGGCAGTCAGGAAAGCAATCGTGGCCGCTGGTGGATGCAGCGATGCGATTC
GTGGTTCCACGTGGCTCGACGCTGTCCGTGACAATGTTTACGACATGGTGGGCAAATCGTTGGTCTCTGAGCTGGCCGTA
GAACACATCATTTTGGACGAGTGGGGGCTCGCAGCCCACACAGATACCACGCTGGCGCGATTGATGGAACAACGCATCGG
CAACCAAATCGCCCAGCTCAAAGGGGCAATGCAGCGCATGCGTCCTTCAGAAGATATGCAAGCATATAACTCTTTGTTTG
CGGATCTCGTGGCTCTGGAACAAACCCGCCGTGAACTTCTAGAAAAGATTTTTAAATAG

Upstream 100 bases:

>100_bases
CTGCAGATTTTTGCATGCGGTGTTGCGGCAGCCAAGGGATTCGACGTGGATCAACCACGTAACTTGGCCAAGTCTGTGAC
GGTGGAGTAGAACACTCACT

Downstream 100 bases:

>100_bases
AAAAAGTGCCGCAAAGGTGTTATCCTTTGCGGCACTTTTTCTATCTTCTTAAGAAGCGAAGGTGACAGGAACCTCGGTCA
CATCGCGGAAACCTTCAGTG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MAGKGRIPDSDIQAIRERTPIEEIVGEYVQLKPAGADSLKGLSPFKEEKTPSFHVRPNHGYFHCFSTGKGGDVFSFLMEM
EQLTFPEAVEVCAEKIGYQINYQGGTTGARREEPGTRKRLIEANRVAHQFYRQQLETPEAAVARQFLLDRGFSQQHIYGF
ECGYAPEGWDTMTKFMLRKGFEFKELEAAGLSTMGRRGPIDRFHRRLLWPIKSVSGDVIGFGARKLFDDDKLGKYMNTPE
TLLYKKSKVLFGLDLAKKSIASQHQAVVVEGYTDVMAMHAAGITTAVAACGTAFGEEHLQILRRYMLDDAYFAGSLIYTF
DGDEAGQKAAMRAFEGDQKFSGQSYVAVAPDGMDPCDLRLAKGDAAVRDLVAQRVPMFEFIIRTLISEYPLDSVEGRLQA
LRRAVPIVASIRDRALKTEYARLLAGWVGWPDPNEVLQQVRAEERRPKTQPRQRRATRIDSNSQPAMIQSMLVMPDRKEP
RLWPQREALKIALQYPEHAGEYFDSLPDDAFTHESYAAVRKAIVAAGGCSDAIRGSTWLDAVRDNVYDMVGKSLVSELAV
EHIILDEWGLAAHTDTTLARLMEQRIGNQIAQLKGAMQRMRPSEDMQAYNSLFADLVALEQTRRELLEKIFK

Sequences:

>Translated_632_residues
MAGKGRIPDSDIQAIRERTPIEEIVGEYVQLKPAGADSLKGLSPFKEEKTPSFHVRPNHGYFHCFSTGKGGDVFSFLMEM
EQLTFPEAVEVCAEKIGYQINYQGGTTGARREEPGTRKRLIEANRVAHQFYRQQLETPEAAVARQFLLDRGFSQQHIYGF
ECGYAPEGWDTMTKFMLRKGFEFKELEAAGLSTMGRRGPIDRFHRRLLWPIKSVSGDVIGFGARKLFDDDKLGKYMNTPE
TLLYKKSKVLFGLDLAKKSIASQHQAVVVEGYTDVMAMHAAGITTAVAACGTAFGEEHLQILRRYMLDDAYFAGSLIYTF
DGDEAGQKAAMRAFEGDQKFSGQSYVAVAPDGMDPCDLRLAKGDAAVRDLVAQRVPMFEFIIRTLISEYPLDSVEGRLQA
LRRAVPIVASIRDRALKTEYARLLAGWVGWPDPNEVLQQVRAEERRPKTQPRQRRATRIDSNSQPAMIQSMLVMPDRKEP
RLWPQREALKIALQYPEHAGEYFDSLPDDAFTHESYAAVRKAIVAAGGCSDAIRGSTWLDAVRDNVYDMVGKSLVSELAV
EHIILDEWGLAAHTDTTLARLMEQRIGNQIAQLKGAMQRMRPSEDMQAYNSLFADLVALEQTRRELLEKIFK
>Mature_631_residues
AGKGRIPDSDIQAIRERTPIEEIVGEYVQLKPAGADSLKGLSPFKEEKTPSFHVRPNHGYFHCFSTGKGGDVFSFLMEME
QLTFPEAVEVCAEKIGYQINYQGGTTGARREEPGTRKRLIEANRVAHQFYRQQLETPEAAVARQFLLDRGFSQQHIYGFE
CGYAPEGWDTMTKFMLRKGFEFKELEAAGLSTMGRRGPIDRFHRRLLWPIKSVSGDVIGFGARKLFDDDKLGKYMNTPET
LLYKKSKVLFGLDLAKKSIASQHQAVVVEGYTDVMAMHAAGITTAVAACGTAFGEEHLQILRRYMLDDAYFAGSLIYTFD
GDEAGQKAAMRAFEGDQKFSGQSYVAVAPDGMDPCDLRLAKGDAAVRDLVAQRVPMFEFIIRTLISEYPLDSVEGRLQAL
RRAVPIVASIRDRALKTEYARLLAGWVGWPDPNEVLQQVRAEERRPKTQPRQRRATRIDSNSQPAMIQSMLVMPDRKEPR
LWPQREALKIALQYPEHAGEYFDSLPDDAFTHESYAAVRKAIVAAGGCSDAIRGSTWLDAVRDNVYDMVGKSLVSELAVE
HIILDEWGLAAHTDTTLARLMEQRIGNQIAQLKGAMQRMRPSEDMQAYNSLFADLVALEQTRRELLEKIFK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=505, Percent_Identity=31.2871287128713, Blast_Score=228, Evalue=9e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 71058; Mature: 70927

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGKGRIPDSDIQAIRERTPIEEIVGEYVQLKPAGADSLKGLSPFKEEKTPSFHVRPNHG
CCCCCCCCCHHHHHHHHCCCHHHHHHHHHEECCCCCHHCCCCCCHHHCCCCCEEECCCCC
YFHCFSTGKGGDVFSFLMEMEQLTFPEAVEVCAEKIGYQINYQGGTTGARREEPGTRKRL
EEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHH
IEANRVAHQFYRQQLETPEAAVARQFLLDRGFSQQHIYGFECGYAPEGWDTMTKFMLRKG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCC
FEFKELEAAGLSTMGRRGPIDRFHRRLLWPIKSVSGDVIGFGARKLFDDDKLGKYMNTPE
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHCCCCH
TLLYKKSKVLFGLDLAKKSIASQHQAVVVEGYTDVMAMHAAGITTAVAACGTAFGEEHLQ
HHHHHHCHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ILRRYMLDDAYFAGSLIYTFDGDEAGQKAAMRAFEGDQKFSGQSYVAVAPDGMDPCDLRL
HHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHEEE
AKGDAAVRDLVAQRVPMFEFIIRTLISEYPLDSVEGRLQALRRAVPIVASIRDRALKTEY
CCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLLAGWVGWPDPNEVLQQVRAEERRPKTQPRQRRATRIDSNSQPAMIQSMLVMPDRKEP
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
RLWPQREALKIALQYPEHAGEYFDSLPDDAFTHESYAAVRKAIVAAGGCSDAIRGSTWLD
CCCCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHH
AVRDNVYDMVGKSLVSELAVEHIILDEWGLAAHTDTTLARLMEQRIGNQIAQLKGAMQRM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
RPSEDMQAYNSLFADLVALEQTRRELLEKIFK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AGKGRIPDSDIQAIRERTPIEEIVGEYVQLKPAGADSLKGLSPFKEEKTPSFHVRPNHG
CCCCCCCCHHHHHHHHCCCHHHHHHHHHEECCCCCHHCCCCCCHHHCCCCCEEECCCCC
YFHCFSTGKGGDVFSFLMEMEQLTFPEAVEVCAEKIGYQINYQGGTTGARREEPGTRKRL
EEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEECCCCCCCCCCCCCHHHHH
IEANRVAHQFYRQQLETPEAAVARQFLLDRGFSQQHIYGFECGYAPEGWDTMTKFMLRKG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHCC
FEFKELEAAGLSTMGRRGPIDRFHRRLLWPIKSVSGDVIGFGARKLFDDDKLGKYMNTPE
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHCCCCH
TLLYKKSKVLFGLDLAKKSIASQHQAVVVEGYTDVMAMHAAGITTAVAACGTAFGEEHLQ
HHHHHHCHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ILRRYMLDDAYFAGSLIYTFDGDEAGQKAAMRAFEGDQKFSGQSYVAVAPDGMDPCDLRL
HHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHEEE
AKGDAAVRDLVAQRVPMFEFIIRTLISEYPLDSVEGRLQALRRAVPIVASIRDRALKTEY
CCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLLAGWVGWPDPNEVLQQVRAEERRPKTQPRQRRATRIDSNSQPAMIQSMLVMPDRKEP
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCC
RLWPQREALKIALQYPEHAGEYFDSLPDDAFTHESYAAVRKAIVAAGGCSDAIRGSTWLD
CCCCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHH
AVRDNVYDMVGKSLVSELAVEHIILDEWGLAAHTDTTLARLMEQRIGNQIAQLKGAMQRM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
RPSEDMQAYNSLFADLVALEQTRRELLEKIFK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9422594 [H]