Click here to switch to the map view.

The map label for this gene is gltX

Identifier: 38233705

GI number: 38233705

Start: 1097919

End: 1099412

Strand: Direct

Name: gltX

Synonym: DIP1115

Alternate gene names: 38233705

Gene position: 1097919-1099412 (Clockwise)

Preceding gene: 38233703

Following gene: 38233706

Centisome position: 44.12

GC content: 52.41

Gene sequence:

>1494_bases
ATGAACATTATGTCTGATGTTCGTGTACGTTTTTGTCCGTCGCCGACCGGCACGCCACATGTTGGTATGGTTCGCACCGC
TTTGTTTAACTGGGCTCACGCTCGTCACACTGGTGGCAAGCTCATTTTTCGTATTGAAGATACGGATGCAGCCCGCGATA
GCGAAGAGTCTTACCAAGCGATTATTGATTCCCTGAAGTGGCTTGGAATGGACTGGGATGAGGGTGTTATTGTTGGTGGC
CCTCATGAACCTTATCGTCAAAGCCAGCGTATGGACATCTACAAGGATGTCTTGGAAAAACTGAAAGAGGCTGGCTTTGT
CTACCCTGCGTACTCGACTGCTCAGGAAGTGGAGGAGCGTCACAAGGCTGCAGGTCGCGACCCTAAGTTGGGCTACGACA
ACTATGACCGTACTTTGACTGATGAGCAGATCGCTGCATTTGAGGCGGAAGGTCGTCAGCCAGTGTGGCGTTTACGTATG
CCGGAGCGGGATTGGAAGTGGAACGATCTGGTTCGTGGTGAGATCGAGTTCAAGAGCTCTACACAGCCTGACTATGTGGT
GGCGCGTTCCAACGGTGCACCGTTGTACACCTTGGTCAACCCAGTTGATGACGCATTGATGGGCATTACCCACGTGCTGC
GTGGCGAGGATCTCTTGCCTTCGACACCTCGTCAGTTGGCTCTTTACGAGGCTCTTAAGGTCATCGGTGTAGCTCAGCAG
ACCCCTGAGTTTGGCCACCTGCCGTTCGTTATGGGTGAGGGTAACAAGAAGCTTTCCAAGCGTGATCCACAGTCCAACCT
GTTTAATCACCGTGATGCCGGCATCATCCCAGAAGGCATGCTGAATTACCTGGCATTGCTGGGCTGGTCACTTGCTGGTG
AAAAAGACATCTTCTCGGTCGATGAGCTTGTAGAAAACTTCGATGTGACCAACGTGTTGGCAAACCCAGCTCGTTTCGAC
CAGAAGAAGCTTGAGGCGATAAACGCTGATCACATCCGTCTTCTTGAGCCTAAGGATTTCGAGCAGCGTTTGCGTGCATA
TCTCACCGAGTACACGGACTTCCCAACAGATTACCCTGCAGAAAAGTTTGCAATCGCCGCAGAGTTGGTGCAAACGCGTA
TCAAGATGCTTGGCGACGCATACGGTTTGCTGAGCTTCCTAGCGATTGCCGATGAGGATCTCACCTTGGACGAGAAGTCG
GCAAAGAAGAACCTCAAGGAGACAGCAATTCCTGCGCTAGATGCTGGTATTGCTGCCCTTGAAGGGGTAGAGGAGTGGAC
CACTCCTGCCATTGAGGCTGCTTTGCATAAGGCGCTGATTGAGGATCTTGACCTCAAGCCTCGTGTTGCTTTTGGTGCTC
TGCGTGTTGGTATCTCAGGCCAGGCTGTGTCGCCACCTCTGTTCGAGTCGATGGAGCTGTTGGGTAAGGAGTCAACGTTG
ACTCGTTTGCGTGCTACCCGTGAGGTCACCCCGTACCAGGTTGCTGCTGAATAA

Upstream 100 bases:

>100_bases
TGGGTACGGATAGAGCCATGGCTACGGGAGAGTAAAAAATCGGGGGCAGTAGCGGGGCGATGGGCACACATGTCTTGCCA
GTCTAGTGCACTAGTCTACG

Downstream 100 bases:

>100_bases
AGCTCATATCAGACATTCCCCTAGGTTAATCGCCTAGGGGATTTGTTGTTTTAGGGAGGAAAAATTGTTGCAACTATTGA
ATATGTTGCTTATGTGACTA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MNIMSDVRVRFCPSPTGTPHVGMVRTALFNWAHARHTGGKLIFRIEDTDAARDSEESYQAIIDSLKWLGMDWDEGVIVGG
PHEPYRQSQRMDIYKDVLEKLKEAGFVYPAYSTAQEVEERHKAAGRDPKLGYDNYDRTLTDEQIAAFEAEGRQPVWRLRM
PERDWKWNDLVRGEIEFKSSTQPDYVVARSNGAPLYTLVNPVDDALMGITHVLRGEDLLPSTPRQLALYEALKVIGVAQQ
TPEFGHLPFVMGEGNKKLSKRDPQSNLFNHRDAGIIPEGMLNYLALLGWSLAGEKDIFSVDELVENFDVTNVLANPARFD
QKKLEAINADHIRLLEPKDFEQRLRAYLTEYTDFPTDYPAEKFAIAAELVQTRIKMLGDAYGLLSFLAIADEDLTLDEKS
AKKNLKETAIPALDAGIAALEGVEEWTTPAIEAALHKALIEDLDLKPRVAFGALRVGISGQAVSPPLFESMELLGKESTL
TRLRATREVTPYQVAAE

Sequences:

>Translated_497_residues
MNIMSDVRVRFCPSPTGTPHVGMVRTALFNWAHARHTGGKLIFRIEDTDAARDSEESYQAIIDSLKWLGMDWDEGVIVGG
PHEPYRQSQRMDIYKDVLEKLKEAGFVYPAYSTAQEVEERHKAAGRDPKLGYDNYDRTLTDEQIAAFEAEGRQPVWRLRM
PERDWKWNDLVRGEIEFKSSTQPDYVVARSNGAPLYTLVNPVDDALMGITHVLRGEDLLPSTPRQLALYEALKVIGVAQQ
TPEFGHLPFVMGEGNKKLSKRDPQSNLFNHRDAGIIPEGMLNYLALLGWSLAGEKDIFSVDELVENFDVTNVLANPARFD
QKKLEAINADHIRLLEPKDFEQRLRAYLTEYTDFPTDYPAEKFAIAAELVQTRIKMLGDAYGLLSFLAIADEDLTLDEKS
AKKNLKETAIPALDAGIAALEGVEEWTTPAIEAALHKALIEDLDLKPRVAFGALRVGISGQAVSPPLFESMELLGKESTL
TRLRATREVTPYQVAAE
>Mature_497_residues
MNIMSDVRVRFCPSPTGTPHVGMVRTALFNWAHARHTGGKLIFRIEDTDAARDSEESYQAIIDSLKWLGMDWDEGVIVGG
PHEPYRQSQRMDIYKDVLEKLKEAGFVYPAYSTAQEVEERHKAAGRDPKLGYDNYDRTLTDEQIAAFEAEGRQPVWRLRM
PERDWKWNDLVRGEIEFKSSTQPDYVVARSNGAPLYTLVNPVDDALMGITHVLRGEDLLPSTPRQLALYEALKVIGVAQQ
TPEFGHLPFVMGEGNKKLSKRDPQSNLFNHRDAGIIPEGMLNYLALLGWSLAGEKDIFSVDELVENFDVTNVLANPARFD
QKKLEAINADHIRLLEPKDFEQRLRAYLTEYTDFPTDYPAEKFAIAAELVQTRIKMLGDAYGLLSFLAIADEDLTLDEKS
AKKNLKETAIPALDAGIAALEGVEEWTTPAIEAALHKALIEDLDLKPRVAFGALRVGISGQAVSPPLFESMELLGKESTL
TRLRATREVTPYQVAAE

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=339, Percent_Identity=36.8731563421829, Blast_Score=207, Evalue=2e-53,
Organism=Escherichia coli, GI1788743, Length=482, Percent_Identity=32.5726141078838, Blast_Score=249, Evalue=4e-67,
Organism=Escherichia coli, GI226510925, Length=324, Percent_Identity=29.0123456790123, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71993394, Length=496, Percent_Identity=28.6290322580645, Blast_Score=192, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6324540, Length=347, Percent_Identity=36.5994236311239, Blast_Score=211, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6321192, Length=265, Percent_Identity=26.4150943396226, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24665321, Length=496, Percent_Identity=30.8467741935484, Blast_Score=228, Evalue=6e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_CORDI (Q6NHM1)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939472.1
- ProteinModelPortal:   Q6NHM1
- SMR:   Q6NHM1
- GeneID:   2650854
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1115
- NMPDR:   fig|257309.1.peg.1063
- HOGENOM:   HBG628189
- OMA:   LPYVMGQ
- ProtClustDB:   PRK01406
- BioCyc:   CDIP257309:DIP1115-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 55745; Mature: 55745

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 260-260

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIMSDVRVRFCPSPTGTPHVGMVRTALFNWAHARHTGGKLIFRIEDTDAARDSEESYQA
CCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHH
IIDSLKWLGMDWDEGVIVGGPHEPYRQSQRMDIYKDVLEKLKEAGFVYPAYSTAQEVEER
HHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHHHHHH
HKAAGRDPKLGYDNYDRTLTDEQIAAFEAEGRQPVWRLRMPERDWKWNDLVRGEIEFKSS
HHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCEEEECCC
TQPDYVVARSNGAPLYTLVNPVDDALMGITHVLRGEDLLPSTPRQLALYEALKVIGVAQQ
CCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
TPEFGHLPFVMGEGNKKLSKRDPQSNLFNHRDAGIIPEGMLNYLALLGWSLAGEKDIFSV
CCCCCCCCEEECCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHH
DELVENFDVTNVLANPARFDQKKLEAINADHIRLLEPKDFEQRLRAYLTEYTDFPTDYPA
HHHHHHCCHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCH
EKFAIAAELVQTRIKMLGDAYGLLSFLAIADEDLTLDEKSAKKNLKETAIPALDAGIAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EGVEEWTTPAIEAALHKALIEDLDLKPRVAFGALRVGISGQAVSPPLFESMELLGKESTL
HHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHEEECCCCCCCCCHHHHHHHHHCCHHHH
TRLRATREVTPYQVAAE
HHHHHHHCCCCEEECCC
>Mature Secondary Structure
MNIMSDVRVRFCPSPTGTPHVGMVRTALFNWAHARHTGGKLIFRIEDTDAARDSEESYQA
CCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHH
IIDSLKWLGMDWDEGVIVGGPHEPYRQSQRMDIYKDVLEKLKEAGFVYPAYSTAQEVEER
HHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEECCCHHHHHHHHHH
HKAAGRDPKLGYDNYDRTLTDEQIAAFEAEGRQPVWRLRMPERDWKWNDLVRGEIEFKSS
HHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCEEEECCC
TQPDYVVARSNGAPLYTLVNPVDDALMGITHVLRGEDLLPSTPRQLALYEALKVIGVAQQ
CCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
TPEFGHLPFVMGEGNKKLSKRDPQSNLFNHRDAGIIPEGMLNYLALLGWSLAGEKDIFSV
CCCCCCCCEEECCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHH
DELVENFDVTNVLANPARFDQKKLEAINADHIRLLEPKDFEQRLRAYLTEYTDFPTDYPA
HHHHHHCCHHHHHCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCH
EKFAIAAELVQTRIKMLGDAYGLLSFLAIADEDLTLDEKSAKKNLKETAIPALDAGIAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
EGVEEWTTPAIEAALHKALIEDLDLKPRVAFGALRVGISGQAVSPPLFESMELLGKESTL
HHHHHHCHHHHHHHHHHHHHHHCCCCCCHHHHHEEECCCCCCCCCHHHHHHHHHCCHHHH
TRLRATREVTPYQVAAE
HHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910