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The map label for this gene is gatB

Identifier: 38233683

GI number: 38233683

Start: 1071435

End: 1072940

Strand: Direct

Name: gatB

Synonym: DIP1089

Alternate gene names: 38233683

Gene position: 1071435-1072940 (Clockwise)

Preceding gene: 38233682

Following gene: 38233684

Centisome position: 43.05

GC content: 52.79

Gene sequence:

>1506_bases
ATGACTGCTGCGATGTATGACCTGATGGATTACGACGAGGTCCTAGAGAAGTTCGATCCGGTAATGGGTCTTGAGGTTCA
CGTTGAGTTAGCAACCGAAACCAAGATGTTTTCGGCACCGTCTGCACATTTCGGCGCTGAACCAAACTCCAACGTAGACC
CAGTATCGTTGGGCTTGCCTGGTGCATTGCCCGTCGTTAATGCTAAAGGCGTTGAATGGGCTATCAAGATCGGTCTGGCT
CTCAACTGCAAGATTGCTGAGTCTTCTCGGTTCGCCCGAAAGAATTACTTCTACCCAGATCAGCCAAAGAACTACCAGAT
TTCGCAGTACGATGAGCCGATTGCTTATGACGGCTACCTTGACGTAGTGCTTGACGACGGAACCCCGTGGCGTGTGGAGA
TCGAGCGCGCCCACATGGAAGAAGACACCGGTAAGCTCACCCACTTGGGTGGTGCCGACGGCCGTATCCACGGTGCGACA
GCTTCGCTCGTCGACTGCAACCGCGCAGGTATCCCGCTTATCGAGATCGTGACCAAACCTATCGAAGGTGCTGGCGAGCG
TGCGCCTGAAGTTGCTCGCGCATACGTTGGAGCTCTTCGTGATTTGGTTAAGGCCTTGGGGGTTTCCGATGCTCGCATGG
ATCAAGGCTCCATGCGTTGCGACGCAAACGTTTCTCTGCGTCCAGTAGGAACTGTTGAGTTCGGTACCCGTACCGAAACT
AAAAACATTAACTCTTTGAAGTCGGTGGAGCAGGCTGTTCGTTATGAGATGCAGCGTCAGGCTCAGGTCCTAGAAGATGG
CGGTGAGATCGTCCAGGAAACTCGTCACTATCAGGAAACCGATGGGTCGACTTCTAAGGGACGTCCAAAGGAAACGGCAG
AGGATTACCGCTACTTTAATGATCCTGATCTGCCACCAGTGATTGCTCCTAAAGAATGGGTAGAGGAGATCCGTGCCACC
CTTCCGGAGCTTCCGTGGATTCGTCGTGCTCGTATTCAAAAAGAGTGGGGGCTTAAAGACGAAGAAATGCGTGACTTGGT
CAACGCAGGTGCTTTGGATCTGATCGTTGAGACAGTCGAAGCTGGTGCATCGGCATCTGAGGCACGCTCGTGGTGGGTTG
CTTACCTTTCACAAAAAGCAAACGAGTCTGGAGTTGAACTTGATTCCTTGTCAATCACTCCGCAGCAGGTAGCTCGCGTT
GCTGCACTGGTGAAGGAAGGAAAGCTGACCAATAAGCTGGCGCGGCAGGCTGTAGACGGAGTTCTTGCCGGTGAGGGTGA
CGTCGATGAGGTCGTTGCTGCTCGTGGCCTCGAAGTGGTTCGTGATGATGGAGCTATTGAAAAGGCAGTGGATGAAGCCT
TGGCCGCTAATCCCGATATTGTTGAGAAATACAAGGCTGGAAACACCAAGGTCACCGGCGCAATTGTTGGTGCAGTGATG
AAGGCTACGCGTGGCAAGGCTGACCCTGCTCAGGTAAATAAACTGATTGCAGAGAAACTCGCATAA

Upstream 100 bases:

>100_bases
GGGAGAATCCACTTTCGGAGAGCTTTCTTCGGAAGTGGCTTTCTTCGTTATAGACCCCCGAATCTACGATTTTTAAGAGA
AGGCATTAGACTATTTCACC

Downstream 100 bases:

>100_bases
CCTCAGCAATTAGGTTGCCCCCTTTGATCGTGTGTATTCGCGCGTTTGAAGGGGGATATTTTTATCTCTTGCCGCGGTGA
GAAGTTGCGCGCACAATAGG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MTAAMYDLMDYDEVLEKFDPVMGLEVHVELATETKMFSAPSAHFGAEPNSNVDPVSLGLPGALPVVNAKGVEWAIKIGLA
LNCKIAESSRFARKNYFYPDQPKNYQISQYDEPIAYDGYLDVVLDDGTPWRVEIERAHMEEDTGKLTHLGGADGRIHGAT
ASLVDCNRAGIPLIEIVTKPIEGAGERAPEVARAYVGALRDLVKALGVSDARMDQGSMRCDANVSLRPVGTVEFGTRTET
KNINSLKSVEQAVRYEMQRQAQVLEDGGEIVQETRHYQETDGSTSKGRPKETAEDYRYFNDPDLPPVIAPKEWVEEIRAT
LPELPWIRRARIQKEWGLKDEEMRDLVNAGALDLIVETVEAGASASEARSWWVAYLSQKANESGVELDSLSITPQQVARV
AALVKEGKLTNKLARQAVDGVLAGEGDVDEVVAARGLEVVRDDGAIEKAVDEALAANPDIVEKYKAGNTKVTGAIVGAVM
KATRGKADPAQVNKLIAEKLA

Sequences:

>Translated_501_residues
MTAAMYDLMDYDEVLEKFDPVMGLEVHVELATETKMFSAPSAHFGAEPNSNVDPVSLGLPGALPVVNAKGVEWAIKIGLA
LNCKIAESSRFARKNYFYPDQPKNYQISQYDEPIAYDGYLDVVLDDGTPWRVEIERAHMEEDTGKLTHLGGADGRIHGAT
ASLVDCNRAGIPLIEIVTKPIEGAGERAPEVARAYVGALRDLVKALGVSDARMDQGSMRCDANVSLRPVGTVEFGTRTET
KNINSLKSVEQAVRYEMQRQAQVLEDGGEIVQETRHYQETDGSTSKGRPKETAEDYRYFNDPDLPPVIAPKEWVEEIRAT
LPELPWIRRARIQKEWGLKDEEMRDLVNAGALDLIVETVEAGASASEARSWWVAYLSQKANESGVELDSLSITPQQVARV
AALVKEGKLTNKLARQAVDGVLAGEGDVDEVVAARGLEVVRDDGAIEKAVDEALAANPDIVEKYKAGNTKVTGAIVGAVM
KATRGKADPAQVNKLIAEKLA
>Mature_500_residues
TAAMYDLMDYDEVLEKFDPVMGLEVHVELATETKMFSAPSAHFGAEPNSNVDPVSLGLPGALPVVNAKGVEWAIKIGLAL
NCKIAESSRFARKNYFYPDQPKNYQISQYDEPIAYDGYLDVVLDDGTPWRVEIERAHMEEDTGKLTHLGGADGRIHGATA
SLVDCNRAGIPLIEIVTKPIEGAGERAPEVARAYVGALRDLVKALGVSDARMDQGSMRCDANVSLRPVGTVEFGTRTETK
NINSLKSVEQAVRYEMQRQAQVLEDGGEIVQETRHYQETDGSTSKGRPKETAEDYRYFNDPDLPPVIAPKEWVEEIRATL
PELPWIRRARIQKEWGLKDEEMRDLVNAGALDLIVETVEAGASASEARSWWVAYLSQKANESGVELDSLSITPQQVARVA
ALVKEGKLTNKLARQAVDGVLAGEGDVDEVVAARGLEVVRDDGAIEKAVDEALAANPDIVEKYKAGNTKVTGAIVGAVMK
ATRGKADPAQVNKLIAEKLA

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=512, Percent_Identity=34.1796875, Blast_Score=292, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI115534094, Length=313, Percent_Identity=39.6166134185303, Blast_Score=212, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6319391, Length=350, Percent_Identity=34, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI45550808, Length=502, Percent_Identity=32.2709163346614, Blast_Score=232, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_CORDI (P61342)

Other databases:

- EMBL:   BX248357
- RefSeq:   NP_939450.1
- ProteinModelPortal:   P61342
- SMR:   P61342
- GeneID:   2650831
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP1089
- NMPDR:   fig|257309.1.peg.1041
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   CDIP257309:DIP1089-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54615; Mature: 54484

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAMYDLMDYDEVLEKFDPVMGLEVHVELATETKMFSAPSAHFGAEPNSNVDPVSLGLP
CCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHCCCCCCCCCCCCCCCCEEEECCC
GALPVVNAKGVEWAIKIGLALNCKIAESSRFARKNYFYPDQPKNYQISQYDEPIAYDGYL
CCCCEECCCCCEEEEEEEEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCEECCEE
DVVLDDGTPWRVEIERAHMEEDTGKLTHLGGADGRIHGATASLVDCNRAGIPLIEIVTKP
EEEECCCCCEEEEEHHHHCCCCCCCEEECCCCCCEEECCEEHEEECCCCCCCHHHHHHCC
IEGAGERAPEVARAYVGALRDLVKALGVSDARMDQGSMRCDANVSLRPVGTVEFGTRTET
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEECCCCEEEECCEEECCCCCCC
KNINSLKSVEQAVRYEMQRQAQVLEDGGEIVQETRHYQETDGSTSKGRPKETAEDYRYFN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCC
DPDLPPVIAPKEWVEEIRATLPELPWIRRARIQKEWGLKDEEMRDLVNAGALDLIVETVE
CCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHH
AGASASEARSWWVAYLSQKANESGVELDSLSITPQQVARVAALVKEGKLTNKLARQAVDG
CCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHH
VLAGEGDVDEVVAARGLEVVRDDGAIEKAVDEALAANPDIVEKYKAGNTKVTGAIVGAVM
HHCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEHHHHHHHHH
KATRGKADPAQVNKLIAEKLA
HHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TAAMYDLMDYDEVLEKFDPVMGLEVHVELATETKMFSAPSAHFGAEPNSNVDPVSLGLP
CCHHHHHHHHHHHHHHHCCCCCEEEEEEEECCHHHHCCCCCCCCCCCCCCCCEEEECCC
GALPVVNAKGVEWAIKIGLALNCKIAESSRFARKNYFYPDQPKNYQISQYDEPIAYDGYL
CCCCEECCCCCEEEEEEEEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCEECCEE
DVVLDDGTPWRVEIERAHMEEDTGKLTHLGGADGRIHGATASLVDCNRAGIPLIEIVTKP
EEEECCCCCEEEEEHHHHCCCCCCCEEECCCCCCEEECCEEHEEECCCCCCCHHHHHHCC
IEGAGERAPEVARAYVGALRDLVKALGVSDARMDQGSMRCDANVSLRPVGTVEFGTRTET
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEECCCCEEEECCEEECCCCCCC
KNINSLKSVEQAVRYEMQRQAQVLEDGGEIVQETRHYQETDGSTSKGRPKETAEDYRYFN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCC
DPDLPPVIAPKEWVEEIRATLPELPWIRRARIQKEWGLKDEEMRDLVNAGALDLIVETVE
CCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHH
AGASASEARSWWVAYLSQKANESGVELDSLSITPQQVARVAALVKEGKLTNKLARQAVDG
CCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHH
VLAGEGDVDEVVAARGLEVVRDDGAIEKAVDEALAANPDIVEKYKAGNTKVTGAIVGAVM
HHCCCCCHHHHHHHCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHCCCCCEEHHHHHHHHH
KATRGKADPAQVNKLIAEKLA
HHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910