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The map label for this gene is pfkA [H]

Identifier: 38233682

GI number: 38233682

Start: 1070298

End: 1071326

Strand: Direct

Name: pfkA [H]

Synonym: DIP1088

Alternate gene names: 38233682

Gene position: 1070298-1071326 (Clockwise)

Preceding gene: 38233681

Following gene: 38233683

Centisome position: 43.01

GC content: 53.64

Gene sequence:

>1029_bases
ATGCGAATTGCGACTCTTACATCCGGCGGCGATTGCCCCGGCCTCAACGCTGTGATCCGCGGCATTGTCCGCACATGTTC
TGAGTACGGCTCGACCGTCGTTGGATACCAAGACGGCTGGGTGGGGCTCATGGAAGACAAGCGCATTCAACTATATGACG
ATGAAAACATCGATCGTATCCTGTTGCGCGGCGGAACCATTTTGGGTACCGGCCGCCTTCACCCTGACAAGTTTAAGTCT
GGTCTTGACCAGATTAAGGCAAACCTTGAGGATGCAGGTATTGATGCACTGATTCCTATCGGTGGCGAAGGTACACTCAA
GGGTGCCAAATGGCTATCTGATAATGGTATTCCTGTAGTTGGCGTGCCTAAGACGATCGACAACGACGTTAACGGTACGG
ACTACACTTTTGGCTTTGATACCGCAGTCTCTGTTGCTACGGATGCTATCGACCGACTGCACACCACAGCTGAGTCTCAC
AACCGTGTGATGATCGTTGAGGTTATGGGTCGTCACGTTGGCTGGATTGCGCTGCACGCCGGCATGGCAGGCGGTGCGCA
TTACACAGTCATTCCTGAGCAGCCATTTGATATTGCAGAGATCTGCAAGGCAATGGAACGCCGCTTCCAGATGGGTGAAA
AGTACGGCATTATCGTCGTGGCGGAAGGTGCATTGCCTAAGGAAGGCACGATGGACTTTGTCGAGGGCGGCGTCGACCAA
TTTGGCCACCAAACGTTTAATGGCATTGGTCAAGTAATTGGTGAGGAAATCAAGAAGCGCCTCGGCCATGACGTTCGTAC
CACGGTTTTGGGTCATATCCAGCGTGGTGGCACACCTACTTCTTATGACCGCGTTTTGGCTACCCGTTACGGTGTTCATG
CCGCACGTGCGTGCCACAACGGCGATTACGGCAAGATGGTTGCGTTGCACGGTGAACATATCGATCTGATTCCGCTCGAG
GACGCGGTAGATACGCTAAAGACTGTCCCGGAAGGACGCTACCGCACAGCACGCGCCCTGTTTGGCTAA

Upstream 100 bases:

>100_bases
GTGGGATATTTACTGTGATTCCCGCCAAAATTCATTCCGATCGGGCGTAATCCCACACATTCTTCAACAATGATTCTCGA
GAAGCGCTACACTGAGAATC

Downstream 100 bases:

>100_bases
AAAATTGGGGGAGAATCCACTTTCGGAGAGCTTTCTTCGGAAGTGGCTTTCTTCGTTATAGACCCCCGAATCTACGATTT
TTAAGAGAAGGCATTAGACT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MRIATLTSGGDCPGLNAVIRGIVRTCSEYGSTVVGYQDGWVGLMEDKRIQLYDDENIDRILLRGGTILGTGRLHPDKFKS
GLDQIKANLEDAGIDALIPIGGEGTLKGAKWLSDNGIPVVGVPKTIDNDVNGTDYTFGFDTAVSVATDAIDRLHTTAESH
NRVMIVEVMGRHVGWIALHAGMAGGAHYTVIPEQPFDIAEICKAMERRFQMGEKYGIIVVAEGALPKEGTMDFVEGGVDQ
FGHQTFNGIGQVIGEEIKKRLGHDVRTTVLGHIQRGGTPTSYDRVLATRYGVHAARACHNGDYGKMVALHGEHIDLIPLE
DAVDTLKTVPEGRYRTARALFG

Sequences:

>Translated_342_residues
MRIATLTSGGDCPGLNAVIRGIVRTCSEYGSTVVGYQDGWVGLMEDKRIQLYDDENIDRILLRGGTILGTGRLHPDKFKS
GLDQIKANLEDAGIDALIPIGGEGTLKGAKWLSDNGIPVVGVPKTIDNDVNGTDYTFGFDTAVSVATDAIDRLHTTAESH
NRVMIVEVMGRHVGWIALHAGMAGGAHYTVIPEQPFDIAEICKAMERRFQMGEKYGIIVVAEGALPKEGTMDFVEGGVDQ
FGHQTFNGIGQVIGEEIKKRLGHDVRTTVLGHIQRGGTPTSYDRVLATRYGVHAARACHNGDYGKMVALHGEHIDLIPLE
DAVDTLKTVPEGRYRTARALFG
>Mature_342_residues
MRIATLTSGGDCPGLNAVIRGIVRTCSEYGSTVVGYQDGWVGLMEDKRIQLYDDENIDRILLRGGTILGTGRLHPDKFKS
GLDQIKANLEDAGIDALIPIGGEGTLKGAKWLSDNGIPVVGVPKTIDNDVNGTDYTFGFDTAVSVATDAIDRLHTTAESH
NRVMIVEVMGRHVGWIALHAGMAGGAHYTVIPEQPFDIAEICKAMERRFQMGEKYGIIVVAEGALPKEGTMDFVEGGVDQ
FGHQTFNGIGQVIGEEIKKRLGHDVRTTVLGHIQRGGTPTSYDRVLATRYGVHAARACHNGDYGKMVALHGEHIDLIPLE
DAVDTLKTVPEGRYRTARALFG

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=374, Percent_Identity=34.4919786096257, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI266453768, Length=378, Percent_Identity=34.9206349206349, Blast_Score=188, Evalue=8e-48,
Organism=Homo sapiens, GI266453748, Length=378, Percent_Identity=34.9206349206349, Blast_Score=188, Evalue=8e-48,
Organism=Homo sapiens, GI4505749, Length=378, Percent_Identity=34.9206349206349, Blast_Score=188, Evalue=8e-48,
Organism=Homo sapiens, GI266453619, Length=378, Percent_Identity=34.9206349206349, Blast_Score=187, Evalue=8e-48,
Organism=Homo sapiens, GI48762920, Length=363, Percent_Identity=32.7823691460055, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1790350, Length=337, Percent_Identity=39.7626112759644, Blast_Score=232, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI25147584, Length=362, Percent_Identity=31.4917127071823, Blast_Score=167, Evalue=9e-42,
Organism=Caenorhabditis elegans, GI17558788, Length=368, Percent_Identity=30.1630434782609, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI72003601, Length=303, Percent_Identity=29.3729372937294, Blast_Score=127, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6321679, Length=362, Percent_Identity=35.0828729281768, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6323861, Length=362, Percent_Identity=34.5303867403315, Blast_Score=170, Evalue=3e-43,
Organism=Drosophila melanogaster, GI28573326, Length=378, Percent_Identity=34.1269841269841, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24652337, Length=378, Percent_Identity=33.8624338624339, Blast_Score=188, Evalue=4e-48,
Organism=Drosophila melanogaster, GI17647809, Length=378, Percent_Identity=33.8624338624339, Blast_Score=188, Evalue=5e-48,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 36979; Mature: 36979

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIATLTSGGDCPGLNAVIRGIVRTCSEYGSTVVGYQDGWVGLMEDKRIQLYDDENIDRI
CEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCEEEEECCCCCCEE
LLRGGTILGTGRLHPDKFKSGLDQIKANLEDAGIDALIPIGGEGTLKGAKWLSDNGIPVV
EEECCEEEECCCCCHHHHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCEEECCCCCEEE
GVPKTIDNDVNGTDYTFGFDTAVSVATDAIDRLHTTAESHNRVMIVEVMGRHVGWIALHA
ECCHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEC
GMAGGAHYTVIPEQPFDIAEICKAMERRFQMGEKYGIIVVAEGALPKEGTMDFVEGGVDQ
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHCCHHH
FGHQTFNGIGQVIGEEIKKRLGHDVRTTVLGHIQRGGTPTSYDRVLATRYGVHAARACHN
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
GDYGKMVALHGEHIDLIPLEDAVDTLKTVPEGRYRTARALFG
CCCCCEEEEECCEEEEEECHHHHHHHHHCCCCCHHHHHHHCC
>Mature Secondary Structure
MRIATLTSGGDCPGLNAVIRGIVRTCSEYGSTVVGYQDGWVGLMEDKRIQLYDDENIDRI
CEEEEECCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCEEEEECCCCCCEE
LLRGGTILGTGRLHPDKFKSGLDQIKANLEDAGIDALIPIGGEGTLKGAKWLSDNGIPVV
EEECCEEEECCCCCHHHHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCEEECCCCCEEE
GVPKTIDNDVNGTDYTFGFDTAVSVATDAIDRLHTTAESHNRVMIVEVMGRHVGWIALHA
ECCHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEC
GMAGGAHYTVIPEQPFDIAEICKAMERRFQMGEKYGIIVVAEGALPKEGTMDFVEGGVDQ
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHCCHHH
FGHQTFNGIGQVIGEEIKKRLGHDVRTTVLGHIQRGGTPTSYDRVLATRYGVHAARACHN
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
GDYGKMVALHGEHIDLIPLEDAVDTLKTVPEGRYRTARALFG
CCCCCEEEEECCEEEEEECHHHHHHHHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]