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The map label for this gene is pcrA [H]

Identifier: 38233432

GI number: 38233432

Start: 811671

End: 814088

Strand: Direct

Name: pcrA [H]

Synonym: DIP0835

Alternate gene names: 38233432

Gene position: 811671-814088 (Clockwise)

Preceding gene: 38233430

Following gene: 38233434

Centisome position: 32.62

GC content: 50.5

Gene sequence:

>2418_bases
ATGGCATCGCAAGCGAATCCGTTTCTCCGCCAAGCTCAGCCCGTATCGCAGTACACCGGTGCCGACCTCACCGAAGGTCT
GAATCCGCAGCAAAAAGCAGCAGTGGAGCATTATGGATCCCCGCTGCTGATCGTTGCAGGCGCTGGATCTGGTAAGACAT
CTGTGCTGACACGGCGAATTGCCTACTTGATGCGTGTTCGAGGTGTACAACCGCAGAACATCTTGGCAATTACGTTTACC
AATAAAGCAGCAGCAGAGATGCGTGAGCGTGTCAGCTCTTTGGTGGGGCCTGTAGCCCAGCGCATGTGGGTATCGACATT
CCACTCCACGTGCGTGCGTATTTTGCGGGAACAGGCACAGCTTGTTCCTGGGCTTAACACTAATTTCACGATCTATGACT
CGGATGATTCCAAGCGATTGTTGTCCATGATCTCCAAGGATTTCAGCTTGGATATTAAGAAGTTCACACCACGGTTGCTG
GCAACTGGAATTTCTAATCTTAAAAATGAGCTGATCGATGCCGACCAAGCATGCGCTGATGCGGAAAGCACGAAGAATCC
CTATGAGACTACGATCGCAAGCGTTTTCGCTGAATATCAAAAGAGACTACGAGCGTCGAACGCCGTCGATTTTGATGACC
TCATCGGGGAGACAGTACGGATTTTCCGTGAGCACCCGCCGGTTACGGAATACTATCGGCGTAGGTTCCGGCACGTGCTT
ATCGACGAGTATCAGGACACTAACCACGCGCAGTATGCATTAATTCATGAGCTTGTTGGAAGTGGACCTGATGCTAGCGA
GTTGTGCGTCGTTGGTGACTCGGACCAGTCAATTTATGCGTTTCGTGGTGCCACAATTCGAAATATTGAGGAATTCGAAC
GCGATTACCCTAATGCTGAGACGATTCTGCTTGAGCAAAATTACCGTTCCACCCAAACGATTCTTAATGCTGCCAATTCG
GTGATCGCTCAAAATGAGAATCGTCGAGAAAAGAAATTATGGACAGCTCTAGGCAGCGGTGATCCCATTGTCGGATATGT
CGCCGACAACGAGCACGATGAAGCCCGTTTTATTGCCAATGAGGTCGACGCGTTGGCTGATAAAGGGGTGGCATATTCCG
ATATTGCTGTCATGTATCGTACGAACAACTCTTCTCGTGCTGTAGAAGACGTCTTTATGCGAACCGGCATACCGTACAAA
GTCGTAGGCGGTACCCGCTTTTATGAGCGTAAAGAGATTCGCGACATCATTGCTTATCTTCGTCTTATTGATAACCCCGA
TGATGCGGTGTCTTTGCGTCGAATCATTAATACTCCACGCCGAGGCATTGGCGATAAAGCCGTAGCATTTCTGTCACTCC
ACGCAGATAACCACAACGTAGGCTTCCACAAAGCATTGCTAGATGCCACCGAAGATAAAGTTTCCATGCTTGGCGCACGT
GGCCGAAATGCTGTCACTGGATTCGTTGACATGATGAAGGGGATTCGCGAAGAGGCCGCAAACAAAGTCAATGAAGTGAC
CGGGATGCCTGATATCGGAGATATGGTGTCCTACATTTTGGACGTCACCGGATACAAAGCCGAATTAGAAAAAAGCAATG
ATCCTCAAGATGGTGCAAGGCTCGATAACCTCAACGAACTGGTATCAGTTGCCCGAGAATTCTCCTCAGAAGCTGCCAAC
CAAGTAGCCTATGAGCGCATGGGGGCGGAGAACCAAGCCCCAGAGATCCCAGAGTTAAGCGAAGGCGAAGCAGCGCCAGG
TAGCCTTATGGCCTTCCTAGAGCGCGTGTCGTTGGTTGCTGATGCCGATCAGATTCCAGATAACGAACAAGGCGTCGTCA
CATTAATGACACTGCACACCGCAAAGGGCCTTGAGTTCCCCGTCGTGTTCCTCACCGGCTGGGAAGACGGTCAGTTCCCG
CATCTGCGTGCTCTAGGAGAGCCTAAGGAGCTAGCAGAAGAGCGTCGACTAGCCTATGTCGGTATTACGCGTGCACGCCG
AACCCTCTACTTGTCCCGCGCGATGCTTCGATCCTCGTGGGGAAATCCAGTGACCAACCCTCCGTCACGGTTCTTATCAG
AAATACCTGATGAACTAATGGATTGGCGTAGAGAAGAACCAGAACACTCCTATGACAACAGCTGGGGTATGGGCTACGGT
TCATCTAATTCGCGTTCTTATGGATCCCAGAACTCTCGTTCTTATGGTTCTCCGCGAACTCCCAAGTTACCTAAGCATTC
CAACAAGCCGGCAATAGCACTCAACGTTGGTGATCGTGTAAACCATGAAAAGTATGGGCTTGGCACAGTAACCAAGGTAG
ACAAGATGGCTCCCGCAGATTCTGTCACCATTGACTTTGGGTCGTCAGGAACAGTGCGGCTAATGCTGATCGGGTCAGTG
CCTATGGAAAAGCTCTAG

Upstream 100 bases:

>100_bases
TGTTGAGGGTTCGTTGTAGGTTGGATAGTTGATGAACAACAACGAACCCTTCTCTCAGTCTCCCTTTGGAACCAACGCAG
CAGCGGCGCCGTCGACGAGC

Downstream 100 bases:

>100_bases
GACGTCTTAGGGGGGACGTGTGTGGGGCGCTTACGAAATAGTCCCGTAAGCGCCCCACACAAAAGTATTTAGATTATTTA
GATAAAGATGCCGTGCTCAG

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 805; Mature: 804

Protein sequence:

>805_residues
MASQANPFLRQAQPVSQYTGADLTEGLNPQQKAAVEHYGSPLLIVAGAGSGKTSVLTRRIAYLMRVRGVQPQNILAITFT
NKAAAEMRERVSSLVGPVAQRMWVSTFHSTCVRILREQAQLVPGLNTNFTIYDSDDSKRLLSMISKDFSLDIKKFTPRLL
ATGISNLKNELIDADQACADAESTKNPYETTIASVFAEYQKRLRASNAVDFDDLIGETVRIFREHPPVTEYYRRRFRHVL
IDEYQDTNHAQYALIHELVGSGPDASELCVVGDSDQSIYAFRGATIRNIEEFERDYPNAETILLEQNYRSTQTILNAANS
VIAQNENRREKKLWTALGSGDPIVGYVADNEHDEARFIANEVDALADKGVAYSDIAVMYRTNNSSRAVEDVFMRTGIPYK
VVGGTRFYERKEIRDIIAYLRLIDNPDDAVSLRRIINTPRRGIGDKAVAFLSLHADNHNVGFHKALLDATEDKVSMLGAR
GRNAVTGFVDMMKGIREEAANKVNEVTGMPDIGDMVSYILDVTGYKAELEKSNDPQDGARLDNLNELVSVAREFSSEAAN
QVAYERMGAENQAPEIPELSEGEAAPGSLMAFLERVSLVADADQIPDNEQGVVTLMTLHTAKGLEFPVVFLTGWEDGQFP
HLRALGEPKELAEERRLAYVGITRARRTLYLSRAMLRSSWGNPVTNPPSRFLSEIPDELMDWRREEPEHSYDNSWGMGYG
SSNSRSYGSQNSRSYGSPRTPKLPKHSNKPAIALNVGDRVNHEKYGLGTVTKVDKMAPADSVTIDFGSSGTVRLMLIGSV
PMEKL

Sequences:

>Translated_805_residues
MASQANPFLRQAQPVSQYTGADLTEGLNPQQKAAVEHYGSPLLIVAGAGSGKTSVLTRRIAYLMRVRGVQPQNILAITFT
NKAAAEMRERVSSLVGPVAQRMWVSTFHSTCVRILREQAQLVPGLNTNFTIYDSDDSKRLLSMISKDFSLDIKKFTPRLL
ATGISNLKNELIDADQACADAESTKNPYETTIASVFAEYQKRLRASNAVDFDDLIGETVRIFREHPPVTEYYRRRFRHVL
IDEYQDTNHAQYALIHELVGSGPDASELCVVGDSDQSIYAFRGATIRNIEEFERDYPNAETILLEQNYRSTQTILNAANS
VIAQNENRREKKLWTALGSGDPIVGYVADNEHDEARFIANEVDALADKGVAYSDIAVMYRTNNSSRAVEDVFMRTGIPYK
VVGGTRFYERKEIRDIIAYLRLIDNPDDAVSLRRIINTPRRGIGDKAVAFLSLHADNHNVGFHKALLDATEDKVSMLGAR
GRNAVTGFVDMMKGIREEAANKVNEVTGMPDIGDMVSYILDVTGYKAELEKSNDPQDGARLDNLNELVSVAREFSSEAAN
QVAYERMGAENQAPEIPELSEGEAAPGSLMAFLERVSLVADADQIPDNEQGVVTLMTLHTAKGLEFPVVFLTGWEDGQFP
HLRALGEPKELAEERRLAYVGITRARRTLYLSRAMLRSSWGNPVTNPPSRFLSEIPDELMDWRREEPEHSYDNSWGMGYG
SSNSRSYGSQNSRSYGSPRTPKLPKHSNKPAIALNVGDRVNHEKYGLGTVTKVDKMAPADSVTIDFGSSGTVRLMLIGSV
PMEKL
>Mature_804_residues
ASQANPFLRQAQPVSQYTGADLTEGLNPQQKAAVEHYGSPLLIVAGAGSGKTSVLTRRIAYLMRVRGVQPQNILAITFTN
KAAAEMRERVSSLVGPVAQRMWVSTFHSTCVRILREQAQLVPGLNTNFTIYDSDDSKRLLSMISKDFSLDIKKFTPRLLA
TGISNLKNELIDADQACADAESTKNPYETTIASVFAEYQKRLRASNAVDFDDLIGETVRIFREHPPVTEYYRRRFRHVLI
DEYQDTNHAQYALIHELVGSGPDASELCVVGDSDQSIYAFRGATIRNIEEFERDYPNAETILLEQNYRSTQTILNAANSV
IAQNENRREKKLWTALGSGDPIVGYVADNEHDEARFIANEVDALADKGVAYSDIAVMYRTNNSSRAVEDVFMRTGIPYKV
VGGTRFYERKEIRDIIAYLRLIDNPDDAVSLRRIINTPRRGIGDKAVAFLSLHADNHNVGFHKALLDATEDKVSMLGARG
RNAVTGFVDMMKGIREEAANKVNEVTGMPDIGDMVSYILDVTGYKAELEKSNDPQDGARLDNLNELVSVAREFSSEAANQ
VAYERMGAENQAPEIPELSEGEAAPGSLMAFLERVSLVADADQIPDNEQGVVTLMTLHTAKGLEFPVVFLTGWEDGQFPH
LRALGEPKELAEERRLAYVGITRARRTLYLSRAMLRSSWGNPVTNPPSRFLSEIPDELMDWRREEPEHSYDNSWGMGYGS
SNSRSYGSQNSRSYGSPRTPKLPKHSNKPAIALNVGDRVNHEKYGLGTVTKVDKMAPADSVTIDFGSSGTVRLMLIGSVP
MEKL

Specific function: Has Both Atpase And Helicase Activities. Unwinds DNA Duplexes With 3' To 5' Polarity With Respect To The Bound Strand And Initiates Unwinding Most Effectively When A Single-Stranded Region Is Present. Involved In The Post-Incision Events Of Nucleotide Exc

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=773, Percent_Identity=37.3868046571798, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI48994965, Length=691, Percent_Identity=38.3502170767004, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1787196, Length=400, Percent_Identity=27.25, Blast_Score=109, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6322369, Length=760, Percent_Identity=28.6842105263158, Blast_Score=245, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6324477, Length=693, Percent_Identity=22.2222222222222, Blast_Score=122, Evalue=2e-28,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 89412; Mature: 89280

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASQANPFLRQAQPVSQYTGADLTEGLNPQQKAAVEHYGSPLLIVAGAGSGKTSVLTRRI
CCCCCCCHHHHCCCHHHHCCCHHHCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
AYLMRVRGVQPQNILAITFTNKAAAEMRERVSSLVGPVAQRMWVSTFHSTCVRILREQAQ
HHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVPGLNTNFTIYDSDDSKRLLSMISKDFSLDIKKFTPRLLATGISNLKNELIDADQACAD
HCCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCC
AESTKNPYETTIASVFAEYQKRLRASNAVDFDDLIGETVRIFREHPPVTEYYRRRFRHVL
CHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IDEYQDTNHAQYALIHELVGSGPDASELCVVGDSDQSIYAFRGATIRNIEEFERDYPNAE
HHHHCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEECCCHHHHHHHHHHHCCCCE
TILLEQNYRSTQTILNAANSVIAQNENRREKKLWTALGSGDPIVGYVADNEHDEARFIAN
EEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHH
EVDALADKGVAYSDIAVMYRTNNSSRAVEDVFMRTGIPYKVVGGTRFYERKEIRDIIAYL
HHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
RLIDNPDDAVSLRRIINTPRRGIGDKAVAFLSLHADNHNVGFHKALLDATEDKVSMLGAR
HHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCC
GRNAVTGFVDMMKGIREEAANKVNEVTGMPDIGDMVSYILDVTGYKAELEKSNDPQDGAR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCH
LDNLNELVSVAREFSSEAANQVAYERMGAENQAPEIPELSEGEAAPGSLMAFLERVSLVA
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
DADQIPDNEQGVVTLMTLHTAKGLEFPVVFLTGWEDGQFPHLRALGEPKELAEERRLAYV
HHHHCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCHHHHCCCHHHHHHHHCEEEE
GITRARRTLYLSRAMLRSSWGNPVTNPPSRFLSEIPDELMDWRREEPEHSYDNSWGMGYG
EHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCC
SSNSRSYGSQNSRSYGSPRTPKLPKHSNKPAIALNVGDRVNHEKYGLGTVTKVDKMAPAD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCCC
SVTIDFGSSGTVRLMLIGSVPMEKL
CEEEEECCCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
ASQANPFLRQAQPVSQYTGADLTEGLNPQQKAAVEHYGSPLLIVAGAGSGKTSVLTRRI
CCCCCCHHHHCCCHHHHCCCHHHCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
AYLMRVRGVQPQNILAITFTNKAAAEMRERVSSLVGPVAQRMWVSTFHSTCVRILREQAQ
HHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVPGLNTNFTIYDSDDSKRLLSMISKDFSLDIKKFTPRLLATGISNLKNELIDADQACAD
HCCCCCCCEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCC
AESTKNPYETTIASVFAEYQKRLRASNAVDFDDLIGETVRIFREHPPVTEYYRRRFRHVL
CHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IDEYQDTNHAQYALIHELVGSGPDASELCVVGDSDQSIYAFRGATIRNIEEFERDYPNAE
HHHHCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEECCCHHHHHHHHHHHCCCCE
TILLEQNYRSTQTILNAANSVIAQNENRREKKLWTALGSGDPIVGYVADNEHDEARFIAN
EEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHH
EVDALADKGVAYSDIAVMYRTNNSSRAVEDVFMRTGIPYKVVGGTRFYERKEIRDIIAYL
HHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
RLIDNPDDAVSLRRIINTPRRGIGDKAVAFLSLHADNHNVGFHKALLDATEDKVSMLGAR
HHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCC
GRNAVTGFVDMMKGIREEAANKVNEVTGMPDIGDMVSYILDVTGYKAELEKSNDPQDGAR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCH
LDNLNELVSVAREFSSEAANQVAYERMGAENQAPEIPELSEGEAAPGSLMAFLERVSLVA
HCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
DADQIPDNEQGVVTLMTLHTAKGLEFPVVFLTGWEDGQFPHLRALGEPKELAEERRLAYV
HHHHCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCHHHHCCCHHHHHHHHCEEEE
GITRARRTLYLSRAMLRSSWGNPVTNPPSRFLSEIPDELMDWRREEPEHSYDNSWGMGYG
EHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCC
SSNSRSYGSQNSRSYGSPRTPKLPKHSNKPAIALNVGDRVNHEKYGLGTVTKVDKMAPAD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCCC
SVTIDFGSSGTVRLMLIGSVPMEKL
CEEEEECCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]