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The map label for this gene is nei [C]

Identifier: 38233426

GI number: 38233426

Start: 804836

End: 805642

Strand: Direct

Name: nei [C]

Synonym: DIP0829

Alternate gene names: 38233426

Gene position: 804836-805642 (Clockwise)

Preceding gene: 38233424

Following gene: 38233428

Centisome position: 32.34

GC content: 53.66

Gene sequence:

>807_bases
ATGCCCGAAGGTGATTCTGTATTTCAACTAGCTCGGCGGCTTAGCTTTATGCAAGGCCGCACCATAACGCATACGAGTCT
TCGGGTACCGGCGTATGCCACCATGCGTTTCGACGGACGCACCATAACAAAAGTGTGGCCCTACGGAAAGCACCTTTTTA
TGCATATCGGTTCAGAAATTTTGCATACGCATCTCAAAATGGAGGGCACATGGGCGGTTCATAGGGCGGGGGATCGGTGG
CGAAAACCCGGCCATACTGCCCGCGTTGTGCTTCACCTTGATGACGCTCCGCATGACCCCATTGAAGTTGTGGGTCACGA
ACTTGGGTTTGTGCGCGTGTTTCCGGACCATGAGTATCCGCAGCGAATCGCCCACCTTGGTCCGGACGTTCTCTCTGAAT
CGTGGCCTACCCGTGGGGAAGCAGAAGCACGAAAACGTCTCCTTGGACAACCCGAACGTGCCATCGGCTTGGCGTTGCTT
GATCAAAAAGTACTAGCAGGTGTGGGAAATGAATACCGCGCCGAAATCTGTTTTATCTGCGGAATCCACCCCGCGACCCG
TATCAAAGACGTGGATGTTGATCGTGTACTTTCGGTTACCCGACGTCTCATGTGGGCCAACCGGTTTTCTCCGATTCGTG
TTACCACTGGTATTCGTCGCCCAGGTGAAACTAGCTATGTTTTTGGGCGCAATCATAAGCCGTGCAGACGCTGCGGCACC
CTCATACGCAAAAGCACGCTTGTCGACGACCCCACCACCGAACTCGAAAGAATCATCTGGTGGTGCCCACTATGTCAAAG
CGAATAG

Upstream 100 bases:

>100_bases
GCGGGTAGGCTTAGCCCCGCCCATTGGGGAAGGATGCGCTGTACTCATACCTACCTTTTGTACCCCCCTCATCAAGATAA
GGCTGCGTGATACTGGTTCT

Downstream 100 bases:

>100_bases
CACAACCTGTGCTGCAATGATCTTAGCGATCATGGCCAGTGGATATACCGAGGTGTAGCCCAATGCCGGCAGATCATTTT
TCGTCATCGCAGAAACATAG

Product: putative endonuclease

Products: NA

Alternate protein names: Putative DNA-(apurinic or apyrimidinic site) lyase SCO5760; Putative AP lyase SCO5760 [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MPEGDSVFQLARRLSFMQGRTITHTSLRVPAYATMRFDGRTITKVWPYGKHLFMHIGSEILHTHLKMEGTWAVHRAGDRW
RKPGHTARVVLHLDDAPHDPIEVVGHELGFVRVFPDHEYPQRIAHLGPDVLSESWPTRGEAEARKRLLGQPERAIGLALL
DQKVLAGVGNEYRAEICFICGIHPATRIKDVDVDRVLSVTRRLMWANRFSPIRVTTGIRRPGETSYVFGRNHKPCRRCGT
LIRKSTLVDDPTTELERIIWWCPLCQSE

Sequences:

>Translated_268_residues
MPEGDSVFQLARRLSFMQGRTITHTSLRVPAYATMRFDGRTITKVWPYGKHLFMHIGSEILHTHLKMEGTWAVHRAGDRW
RKPGHTARVVLHLDDAPHDPIEVVGHELGFVRVFPDHEYPQRIAHLGPDVLSESWPTRGEAEARKRLLGQPERAIGLALL
DQKVLAGVGNEYRAEICFICGIHPATRIKDVDVDRVLSVTRRLMWANRFSPIRVTTGIRRPGETSYVFGRNHKPCRRCGT
LIRKSTLVDDPTTELERIIWWCPLCQSE
>Mature_267_residues
PEGDSVFQLARRLSFMQGRTITHTSLRVPAYATMRFDGRTITKVWPYGKHLFMHIGSEILHTHLKMEGTWAVHRAGDRWR
KPGHTARVVLHLDDAPHDPIEVVGHELGFVRVFPDHEYPQRIAHLGPDVLSESWPTRGEAEARKRLLGQPERAIGLALLD
QKVLAGVGNEYRAEICFICGIHPATRIKDVDVDRVLSVTRRLMWANRFSPIRVTTGIRRPGETSYVFGRNHKPCRRCGTL
IRKSTLVDDPTTELERIIWWCPLCQSE

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA b

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786932, Length=280, Percent_Identity=28.9285714285714, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR012319
- InterPro:   IPR010979
- InterPro:   IPR000214 [H]

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 30677; Mature: 30546

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS51066 ZF_FPG_2 ; PS51068 FPG_CAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGDSVFQLARRLSFMQGRTITHTSLRVPAYATMRFDGRTITKVWPYGKHLFMHIGSEI
CCCCHHHHHHHHHHHHHCCCEEEECEEECCEEEEEEECCEEEEEECCCHHHHHHHHHHHH
LHTHLKMEGTWAVHRAGDRWRKPGHTARVVLHLDDAPHDPIEVVGHELGFVRVFPDHEYP
HHHHHCCCCCEEEECCCHHHCCCCCEEEEEEEECCCCCCHHHHHHCCCCEEEECCCCCHH
QRIAHLGPDVLSESWPTRGEAEARKRLLGQPERAIGLALLDQKVLAGVGNEYRAEICFIC
HHHHHCCHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEE
GIHPATRIKDVDVDRVLSVTRRLMWANRFSPIRVTTGIRRPGETSYVFGRNHKPCRRCGT
CCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCCCCHHHHHHH
LIRKSTLVDDPTTELERIIWWCPLCQSE
HHHHHHCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PEGDSVFQLARRLSFMQGRTITHTSLRVPAYATMRFDGRTITKVWPYGKHLFMHIGSEI
CCCHHHHHHHHHHHHHCCCEEEECEEECCEEEEEEECCEEEEEECCCHHHHHHHHHHHH
LHTHLKMEGTWAVHRAGDRWRKPGHTARVVLHLDDAPHDPIEVVGHELGFVRVFPDHEYP
HHHHHCCCCCEEEECCCHHHCCCCCEEEEEEEECCCCCCHHHHHHCCCCEEEECCCCCHH
QRIAHLGPDVLSESWPTRGEAEARKRLLGQPERAIGLALLDQKVLAGVGNEYRAEICFIC
HHHHHCCHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEE
GIHPATRIKDVDVDRVLSVTRRLMWANRFSPIRVTTGIRRPGETSYVFGRNHKPCRRCGT
CCCCHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCCCCHHHHHHH
LIRKSTLVDDPTTELERIIWWCPLCQSE
HHHHHHCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]