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The map label for this gene is prfB

Identifier: 38233352

GI number: 38233352

Start: 721938

End: 723047

Strand: Direct

Name: prfB

Synonym: DIP0746

Alternate gene names: 38233352

Gene position: 721938-723047 (Clockwise)

Preceding gene: 38233349

Following gene: 38233354

Centisome position: 29.01

GC content: 53.87

Gene sequence:

>1110_bases
ATGCGACCGGAAACTCAATCTGCTGTAACTCAACTCGACTCGACTTTGACGACCATCGAAAAAGTGATGAATCCCGAGGA
TTTGGAGTCGCGTGTTCGCGAGTTGGAACAGCAAGCCTCCGATCCTACGCTGTGGGACGATCCTGACACCGCGCAAAAGG
TGACTACCGAGTTGTCTTCGGTGCAAGCCAAGCTGAAAAAGCTCAGCACATTGCGCCAGCGTATCGACGACCTCCCCGTG
ATGTATGAGCTCGCCGATGAGGAAGGCGAGGATGCTTTAGCGCTTGCCGACGAAGAACTCGCCGAGCTGACCGCAGACGT
TGAAGCCTTAGAAGTAAAGACTATGTTGTCTGGCGAGTACGATTCCCGTGAGGCAGTGATCAATATCCGTTCTGGCGCCG
GTGGCGTGGATGCCGCTGACTGGGCGGAAATGCTCATGCGTATGTACATCCGCTGGGCTGAGAAAAACGACCACAAAGTA
GATATTTACGACATTTCTTATGCCGAAGAAGCAGGCATTAAGTCCGCAACGTTCGTAGTTCATGGTGAGTACATGTACGG
ACAATTATCGGTCGAACAGGGTGCTCACCGCTTGGTTCGTATTAGTCCTTTTGATAACCAGGGGCGTCGCCAGACCTCTT
TCGCTGAGATCGAGGTTCTCCCCGTTGTTGAACAAACAGACCACATCGATATCCCTGACTCCGAAGTGCGTGTCGACGTC
TACCGTTCCTCGGGGCCAGGTGGGCAGTCCGTGAACACCACCGACTCGGCCGTGCGTCTAACGCACATCCCAACGGGCAT
CGTCGTGACGTGTCAGAATGAGAAGTCCCAGATCCAGAACAAGGCATCAGCCATGCGCGTTCTTCAAGCAAAACTTTTGG
AGCGTAAGCGCCAAGAAGAGCGTGCCGAACTTGATGCGCTCGGCGCAGGCGGTAACGCCTCATGGGGCAACCAAATGCGC
TCCTATGTGCTGCACCCATATCAAATGGTAAAAGATCTGCGTACAAACTATGAGGTCAATGACCCCCAGAAGGTTCTCGA
CGGCGACATCGATGGCCTCCTAGAAGCAGGTATTCGCTGGCGCATGGCGGAACAACAGGGGCAAAACTAA

Upstream 100 bases:

>100_bases
TAGGAATGGAGCCAACATTTCCCCACGGGGGTCTGCCCACTTGAGGGGGAGATCAGGCCAGTATCAAACACGGCGGGCAG
ACACGCTAGAATTATCGCTT

Downstream 100 bases:

>100_bases
CCCTGCTGGAAGTGCATAGGGGTTCCAAGACCCAGCGGAATCCCTAGTGCGTACCAAACAATAAAGAAGGCAAACCAGCC
AACAAGCATCGCAATGGAAT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MRPETQSAVTQLDSTLTTIEKVMNPEDLESRVRELEQQASDPTLWDDPDTAQKVTTELSSVQAKLKKLSTLRQRIDDLPV
MYELADEEGEDALALADEELAELTADVEALEVKTMLSGEYDSREAVINIRSGAGGVDAADWAEMLMRMYIRWAEKNDHKV
DIYDISYAEEAGIKSATFVVHGEYMYGQLSVEQGAHRLVRISPFDNQGRRQTSFAEIEVLPVVEQTDHIDIPDSEVRVDV
YRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQIQNKASAMRVLQAKLLERKRQEERAELDALGAGGNASWGNQMR
SYVLHPYQMVKDLRTNYEVNDPQKVLDGDIDGLLEAGIRWRMAEQQGQN

Sequences:

>Translated_369_residues
MRPETQSAVTQLDSTLTTIEKVMNPEDLESRVRELEQQASDPTLWDDPDTAQKVTTELSSVQAKLKKLSTLRQRIDDLPV
MYELADEEGEDALALADEELAELTADVEALEVKTMLSGEYDSREAVINIRSGAGGVDAADWAEMLMRMYIRWAEKNDHKV
DIYDISYAEEAGIKSATFVVHGEYMYGQLSVEQGAHRLVRISPFDNQGRRQTSFAEIEVLPVVEQTDHIDIPDSEVRVDV
YRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQIQNKASAMRVLQAKLLERKRQEERAELDALGAGGNASWGNQMR
SYVLHPYQMVKDLRTNYEVNDPQKVLDGDIDGLLEAGIRWRMAEQQGQN
>Mature_369_residues
MRPETQSAVTQLDSTLTTIEKVMNPEDLESRVRELEQQASDPTLWDDPDTAQKVTTELSSVQAKLKKLSTLRQRIDDLPV
MYELADEEGEDALALADEELAELTADVEALEVKTMLSGEYDSREAVINIRSGAGGVDAADWAEMLMRMYIRWAEKNDHKV
DIYDISYAEEAGIKSATFVVHGEYMYGQLSVEQGAHRLVRISPFDNQGRRQTSFAEIEVLPVVEQTDHIDIPDSEVRVDV
YRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQIQNKASAMRVLQAKLLERKRQEERAELDALGAGGNASWGNQMR
SYVLHPYQMVKDLRTNYEVNDPQKVLDGDIDGLLEAGIRWRMAEQQGQN

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=275, Percent_Identity=31.6363636363636, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI34577120, Length=346, Percent_Identity=30.0578034682081, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI166795305, Length=186, Percent_Identity=30.6451612903226, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI2367172, Length=331, Percent_Identity=45.3172205438066, Blast_Score=283, Evalue=1e-77,
Organism=Escherichia coli, GI1787462, Length=250, Percent_Identity=39.2, Blast_Score=174, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI17542784, Length=275, Percent_Identity=32, Blast_Score=104, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6321295, Length=366, Percent_Identity=31.4207650273224, Blast_Score=137, Evalue=4e-33,
Organism=Drosophila melanogaster, GI19921226, Length=232, Percent_Identity=31.8965517241379, Blast_Score=121, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_CORDI (Q6NIM2)

Other databases:

- EMBL:   BX248356
- RefSeq:   NP_939119.1
- ProteinModelPortal:   Q6NIM2
- SMR:   Q6NIM2
- GeneID:   2650548
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0746
- NMPDR:   fig|257309.1.peg.710
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- ProtClustDB:   PRK00578
- BioCyc:   CDIP257309:DIP0746-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41330; Mature: 41330

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPETQSAVTQLDSTLTTIEKVMNPEDLESRVRELEQQASDPTLWDDPDTAQKVTTELSS
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
VQAKLKKLSTLRQRIDDLPVMYELADEEGEDALALADEELAELTADVEALEVKTMLSGEY
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DSREAVINIRSGAGGVDAADWAEMLMRMYIRWAEKNDHKVDIYDISYAEEAGIKSATFVV
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCEEEEEE
HGEYMYGQLSVEQGAHRLVRISPFDNQGRRQTSFAEIEVLPVVEQTDHIDIPDSEVRVDV
ECCEEEEEEEHHCCCCEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEE
YRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQIQNKASAMRVLQAKLLERKRQEE
EECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAELDALGAGGNASWGNQMRSYVLHPYQMVKDLRTNYEVNDPQKVLDGDIDGLLEAGIRW
HHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHCHHE
RMAEQQGQN
EEHHHCCCC
>Mature Secondary Structure
MRPETQSAVTQLDSTLTTIEKVMNPEDLESRVRELEQQASDPTLWDDPDTAQKVTTELSS
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
VQAKLKKLSTLRQRIDDLPVMYELADEEGEDALALADEELAELTADVEALEVKTMLSGEY
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DSREAVINIRSGAGGVDAADWAEMLMRMYIRWAEKNDHKVDIYDISYAEEAGIKSATFVV
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHCCCCEEEEEE
HGEYMYGQLSVEQGAHRLVRISPFDNQGRRQTSFAEIEVLPVVEQTDHIDIPDSEVRVDV
ECCEEEEEEEHHCCCCEEEEECCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEE
YRSSGPGGQSVNTTDSAVRLTHIPTGIVVTCQNEKSQIQNKASAMRVLQAKLLERKRQEE
EECCCCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAELDALGAGGNASWGNQMRSYVLHPYQMVKDLRTNYEVNDPQKVLDGDIDGLLEAGIRW
HHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHCHHE
RMAEQQGQN
EEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14602910