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The map label for this gene is pycB

Identifier: 38233345

GI number: 38233345

Start: 715065

End: 716546

Strand: Direct

Name: pycB

Synonym: DIP0739

Alternate gene names: NA

Gene position: 715065-716546 (Clockwise)

Preceding gene: 38233344

Following gene: 38233346

Centisome position: 28.73

GC content: 53.98

Gene sequence:

>1482_bases
ATGAGCCCGAGAAAAATTGGAGTGACCGAAGTCGCTCTGCGCGACGCACATCAGAGCTTGTTTGCAACCCGCATGGCCAT
GGAAGACATGGTCGCCTCATGCGAGGATATTGACAACGCAGGCTTTTGGAGTGTGGAGTGCTGGGGCGGCGCAACGTACG
ACGCCTGCATCCGATTCCTCAACGAAGATCCATGGGAGCGCTTGCGTACCTTCCGCAAGTTGATGCCTAATTCGAAGCTG
CAGATGCTGCTTCGTGGCCAGAATCTTCTAGGATACCGCCACTATGAGGACACTGTGGTGGACAAGTTTGTGGAGAAGTC
AAAGGAAAACGGCATGGACGTTTTCCGTGTCTTTGATGCTTTGAACGATCCGCGCAACATGGAACGCGCAATGCAAGCGG
TGAAGAAAGTGGGCGGCCACGCCCAAGGCACGATCTGCTACACCGTGTCGCCACTGCACACCGTAGAAGGATACATTGAG
CAGGCAGGTCGACTGCTCGACATGGGTGCAGATTCCATTGCTTTGAAGGATATGGCTGCGCTTTTGAAGCCACAGCCTGC
CTATGATGTCATTCGCGGTATCAAGGAAACCTACGGCGAAGACACTCAGATCAATGTCCACTGCCACTCGACCACTGGTG
TTACCTTGGTGACCTTGATGAAGGCCATTGAGGCTGGCGCAGATGTTGTCGACACCGCAATTTCTTCCTTGTCGCTCGGC
CCAGGCCACAACCCAACCGAGTCTCTCGTTGAAATGCTTGAGGGTACCGAGTACACCACAGATCTGGACATGGATCGTCT
GATTAAGATTCGTGACCACTTCAAGACAATTCGCCCGAAGTACAAAGAGTTTGAGTCCAAGACGTTGGTGGATACCAACA
TCTTCCTTTCACAGATTCCTGGCGGCATGCTCTCCAACATGGAAAGCCAGCTCACCGCTCAAGGTGCAGGCGACCGTATC
GATGAAGTGATGCGCGAAGTTCCAATCGTTCGTAAAGATGCTGGTTACCCACCACTCGTGACGCCTTCTTCGCAGATCGT
CGGCACGCAAGCAGTGTTCAACGTTCTCATGGGCCGTTACAAGGTCATGACCGCAGAGTTCGCCGACTTGATGCTGGGTT
ACTACGGCGAATGCATCGGTGAGCGCAACGCTGAATTGGTTGAGCAGGCAAAGGCTCAAACCAAGAAGGAACAGATCACC
GTTCGTCCTGCAGACCTTCTCGAGCCAGAGTGGGATCACCTCGTCGAAGAGGCATCCAAGCTGGAAGGTTTTGATGGTAC
CGATGAGGACGTGCTCACCAACGCACTGTTCCCAGGTGTTGCACCAGGGTTCTTTAAGACCCGCCCAGAGGGGCCAAAGA
ACGTGGGCAAGGATCCATCGAAGATCAAGACCCGCGATAACCAAGCGGTCTTGGAACCAATCACCTACAAGGTCACCGTG
GGCGGTCGCAGCCAGACCGTCAAGGTTGAGCCAGCTGAATAA

Upstream 100 bases:

>100_bases
AAGTGGACACGGTCATATGTTTGGGTTTGGTTGGTTTTGTGATCTTTTCCTAGTGTTTTCAACGTGCGTTACAGGACAAA
AATGCCTAAATTTGTGAGTT

Downstream 100 bases:

>100_bases
GGAGTTTGACTAATGGCTAAAGAATTGTCGATGGCCGAGCGCCTCGAAAAGCTGGCAGCTGCTAAGGCCGAGGTAAGCCT
TGGCGGTGGTCAAGCCAAGA

Product: oxaloacetate decarboxylase

Products: NA

Alternate protein names: Transcarboxylase 5S subunit [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSPRKIGVTEVALRDAHQSLFATRMAMEDMVASCEDIDNAGFWSVECWGGATYDACIRFLNEDPWERLRTFRKLMPNSKL
QMLLRGQNLLGYRHYEDTVVDKFVEKSKENGMDVFRVFDALNDPRNMERAMQAVKKVGGHAQGTICYTVSPLHTVEGYIE
QAGRLLDMGADSIALKDMAALLKPQPAYDVIRGIKETYGEDTQINVHCHSTTGVTLVTLMKAIEAGADVVDTAISSLSLG
PGHNPTESLVEMLEGTEYTTDLDMDRLIKIRDHFKTIRPKYKEFESKTLVDTNIFLSQIPGGMLSNMESQLTAQGAGDRI
DEVMREVPIVRKDAGYPPLVTPSSQIVGTQAVFNVLMGRYKVMTAEFADLMLGYYGECIGERNAELVEQAKAQTKKEQIT
VRPADLLEPEWDHLVEEASKLEGFDGTDEDVLTNALFPGVAPGFFKTRPEGPKNVGKDPSKIKTRDNQAVLEPITYKVTV
GGRSQTVKVEPAE

Sequences:

>Translated_493_residues
MSPRKIGVTEVALRDAHQSLFATRMAMEDMVASCEDIDNAGFWSVECWGGATYDACIRFLNEDPWERLRTFRKLMPNSKL
QMLLRGQNLLGYRHYEDTVVDKFVEKSKENGMDVFRVFDALNDPRNMERAMQAVKKVGGHAQGTICYTVSPLHTVEGYIE
QAGRLLDMGADSIALKDMAALLKPQPAYDVIRGIKETYGEDTQINVHCHSTTGVTLVTLMKAIEAGADVVDTAISSLSLG
PGHNPTESLVEMLEGTEYTTDLDMDRLIKIRDHFKTIRPKYKEFESKTLVDTNIFLSQIPGGMLSNMESQLTAQGAGDRI
DEVMREVPIVRKDAGYPPLVTPSSQIVGTQAVFNVLMGRYKVMTAEFADLMLGYYGECIGERNAELVEQAKAQTKKEQIT
VRPADLLEPEWDHLVEEASKLEGFDGTDEDVLTNALFPGVAPGFFKTRPEGPKNVGKDPSKIKTRDNQAVLEPITYKVTV
GGRSQTVKVEPAE
>Mature_492_residues
SPRKIGVTEVALRDAHQSLFATRMAMEDMVASCEDIDNAGFWSVECWGGATYDACIRFLNEDPWERLRTFRKLMPNSKLQ
MLLRGQNLLGYRHYEDTVVDKFVEKSKENGMDVFRVFDALNDPRNMERAMQAVKKVGGHAQGTICYTVSPLHTVEGYIEQ
AGRLLDMGADSIALKDMAALLKPQPAYDVIRGIKETYGEDTQINVHCHSTTGVTLVTLMKAIEAGADVVDTAISSLSLGP
GHNPTESLVEMLEGTEYTTDLDMDRLIKIRDHFKTIRPKYKEFESKTLVDTNIFLSQIPGGMLSNMESQLTAQGAGDRID
EVMREVPIVRKDAGYPPLVTPSSQIVGTQAVFNVLMGRYKVMTAEFADLMLGYYGECIGERNAELVEQAKAQTKKEQITV
RPADLLEPEWDHLVEEASKLEGFDGTDEDVLTNALFPGVAPGFFKTRPEGPKNVGKDPSKIKTRDNQAVLEPITYKVTVG
GRSQTVKVEPAE

Specific function: The 5S subunit specifically catalyzes the transfer of the carboxyl group from biotin of the 1.3S subunit to pyruvate to form oxaloacetate and 1.3S biotin [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=457, Percent_Identity=33.6980306345733, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI106049295, Length=457, Percent_Identity=33.6980306345733, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI106049292, Length=457, Percent_Identity=33.6980306345733, Blast_Score=238, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17562816, Length=481, Percent_Identity=35.5509355509356, Blast_Score=255, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6319695, Length=453, Percent_Identity=34.6578366445916, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6321376, Length=453, Percent_Identity=34.6578366445916, Blast_Score=246, Evalue=4e-66,
Organism=Drosophila melanogaster, GI281363050, Length=468, Percent_Identity=34.6153846153846, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24652224, Length=468, Percent_Identity=34.6153846153846, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24652222, Length=468, Percent_Identity=34.6153846153846, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24652220, Length=468, Percent_Identity=34.6153846153846, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24652218, Length=468, Percent_Identity=34.6153846153846, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24652212, Length=468, Percent_Identity=34.6153846153846, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24652210, Length=468, Percent_Identity=34.6153846153846, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24652214, Length=468, Percent_Identity=34.6153846153846, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI19921944, Length=468, Percent_Identity=34.6153846153846, Blast_Score=242, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24652216, Length=468, Percent_Identity=34.6153846153846, Blast_Score=242, Evalue=5e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR003379
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =2.1.3.1 [H]

Molecular weight: Translated: 54786; Mature: 54655

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRKIGVTEVALRDAHQSLFATRMAMEDMVASCEDIDNAGFWSVECWGGATYDACIRFL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHH
NEDPWERLRTFRKLMPNSKLQMLLRGQNLLGYRHYEDTVVDKFVEKSKENGMDVFRVFDA
CCCHHHHHHHHHHHCCCHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
LNDPRNMERAMQAVKKVGGHAQGTICYTVSPLHTVEGYIEQAGRLLDMGADSIALKDMAA
HCCCHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHCCHHHHCCCCHHHHHHHHH
LLKPQPAYDVIRGIKETYGEDTQINVHCHSTTGVTLVTLMKAIEAGADVVDTAISSLSLG
HHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHCCCC
PGHNPTESLVEMLEGTEYTTDLDMDRLIKIRDHFKTIRPKYKEFESKTLVDTNIFLSQIP
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCEEEHHHHHHHCC
GGMLSNMESQLTAQGAGDRIDEVMREVPIVRKDAGYPPLVTPSSQIVGTQAVFNVLMGRY
CHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KVMTAEFADLMLGYYGECIGERNAELVEQAKAQTKKEQITVRPADLLEPEWDHLVEEASK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCHHHCCCCHHHHHHHHHH
LEGFDGTDEDVLTNALFPGVAPGFFKTRPEGPKNVGKDPSKIKTRDNQAVLEPITYKVTV
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEECCEEEEEEE
GGRSQTVKVEPAE
CCCCEEEEECCCC
>Mature Secondary Structure 
SPRKIGVTEVALRDAHQSLFATRMAMEDMVASCEDIDNAGFWSVECWGGATYDACIRFL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHH
NEDPWERLRTFRKLMPNSKLQMLLRGQNLLGYRHYEDTVVDKFVEKSKENGMDVFRVFDA
CCCHHHHHHHHHHHCCCHHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
LNDPRNMERAMQAVKKVGGHAQGTICYTVSPLHTVEGYIEQAGRLLDMGADSIALKDMAA
HCCCHHHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHHHHHCCHHHHCCCCHHHHHHHHH
LLKPQPAYDVIRGIKETYGEDTQINVHCHSTTGVTLVTLMKAIEAGADVVDTAISSLSLG
HHCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCHHHHHHHHHHCCCC
PGHNPTESLVEMLEGTEYTTDLDMDRLIKIRDHFKTIRPKYKEFESKTLVDTNIFLSQIP
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCEEEHHHHHHHCC
GGMLSNMESQLTAQGAGDRIDEVMREVPIVRKDAGYPPLVTPSSQIVGTQAVFNVLMGRY
CHHHHHHHHHHHHCCCCHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHH
KVMTAEFADLMLGYYGECIGERNAELVEQAKAQTKKEQITVRPADLLEPEWDHLVEEASK
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEECCHHHCCCCHHHHHHHHHH
LEGFDGTDEDVLTNALFPGVAPGFFKTRPEGPKNVGKDPSKIKTRDNQAVLEPITYKVTV
CCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCEECCEEEEEEE
GGRSQTVKVEPAE
CCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8365490 [H]