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The map label for this gene is hemB [H]

Identifier: 38233017

GI number: 38233017

Start: 368725

End: 369717

Strand: Direct

Name: hemB [H]

Synonym: DIP0403

Alternate gene names: 38233017

Gene position: 368725-369717 (Clockwise)

Preceding gene: 38233016

Following gene: 38233019

Centisome position: 14.82

GC content: 53.58

Gene sequence:

>993_bases
ATGAATCTAGATCTTATTCGTCGTCCTCGTCGGTTGCGTTCGACTCCGGTGATGCGTGAATTTGTTGCGGAAACCACGCT
GCGTCCGGCTGATCTTATTTTGCCGATGTTTATTGCCGATGGCATTGATAAGCCTCGGGAGATTGAGTCGATGCCAGGTG
TGTACCAGCACACCATGGAATCCCTTATAGAGGCTGTCCGTGAGGCGATCTCGGTGGGTATTCGTTGCGTGGATCTTTTT
GGTGTGCCACTGGATTCCGATAAGGATGCCACTGGTTCACAAGCGTGGGATCCCGAGGGCATTTTGAACCGCGGTGTGCG
TGCGCTTCGTCAGGAGTTCGGTGATCAGGTGCTCATCATGGCGGATACCTGCCTTGACGAGTTCACCGATCATGGGCACT
GTGGCATTTTGACCACGGATCGCTTTGGCGCCACCATTGTGGATAACGATCCAACGGTTGAGTTGTATCAGAAGATGGCG
GTTGCTCAGGCTGAAGCGGGTGCTCATATTGTGAGCCCGTCGGGCATGATGGACGGCCAGATTTTAGCTATCCGTGAGGC
TCTCGACGCCGCAGGGTTCCACGATGTTGCGATCATGGCGTATTCCGCTAAATATGCGTCGGCGTTTTATGGTCCTTTCC
GTGACGCAGTGGGCTCCTCTTTGCAGGGTGATCGTCGTGCCTACCAGCAGGATCCTGCTAATCTCCGTGAGTCGATCCTC
GAAGTCGATCTGGACATCGAGGAAGGCGCGGATTTTGTTATGGTCAAACCTGCTATGCCGTACTTGGATGTGCTTGCTGA
GGTAGCGTCTACGTCGTCGGTTCCGGTGGCGGCTTACCAGGTTTCTGGCGAGTATGCGATGTTGCATGCGGCTGCTGCGA
ACGGCTGGTTGGATCTGGATCGGGTGATGATGGAGTCTCTGATTTCTATTAAACGTGCCGGTGCGGATCAAATCCTGACA
TACTTTGCTGTAGAAGCAGCACGAAAACTTTAA

Upstream 100 bases:

>100_bases
ATGCGCTCGCGGAGCATGTGGAGAAACTGCGTGCTGCGGGTGAGTTGCCGCCGCCGCGTAAGAAGCGTCGGGCCCGTCGC
AAATCTAAGGAAGGTGCTGT

Downstream 100 bases:

>100_bases
GGGAGCGCGTCGAGTGACAACTCAACCCGAGGCCGTGCGTTGGGCCGCCGCATCGTGGTGGACGGCGCTGGCGATTAACG
CAGTTCATCAGATTATTGGT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MNLDLIRRPRRLRSTPVMREFVAETTLRPADLILPMFIADGIDKPREIESMPGVYQHTMESLIEAVREAISVGIRCVDLF
GVPLDSDKDATGSQAWDPEGILNRGVRALRQEFGDQVLIMADTCLDEFTDHGHCGILTTDRFGATIVDNDPTVELYQKMA
VAQAEAGAHIVSPSGMMDGQILAIREALDAAGFHDVAIMAYSAKYASAFYGPFRDAVGSSLQGDRRAYQQDPANLRESIL
EVDLDIEEGADFVMVKPAMPYLDVLAEVASTSSVPVAAYQVSGEYAMLHAAAANGWLDLDRVMMESLISIKRAGADQILT
YFAVEAARKL

Sequences:

>Translated_330_residues
MNLDLIRRPRRLRSTPVMREFVAETTLRPADLILPMFIADGIDKPREIESMPGVYQHTMESLIEAVREAISVGIRCVDLF
GVPLDSDKDATGSQAWDPEGILNRGVRALRQEFGDQVLIMADTCLDEFTDHGHCGILTTDRFGATIVDNDPTVELYQKMA
VAQAEAGAHIVSPSGMMDGQILAIREALDAAGFHDVAIMAYSAKYASAFYGPFRDAVGSSLQGDRRAYQQDPANLRESIL
EVDLDIEEGADFVMVKPAMPYLDVLAEVASTSSVPVAAYQVSGEYAMLHAAAANGWLDLDRVMMESLISIKRAGADQILT
YFAVEAARKL
>Mature_330_residues
MNLDLIRRPRRLRSTPVMREFVAETTLRPADLILPMFIADGIDKPREIESMPGVYQHTMESLIEAVREAISVGIRCVDLF
GVPLDSDKDATGSQAWDPEGILNRGVRALRQEFGDQVLIMADTCLDEFTDHGHCGILTTDRFGATIVDNDPTVELYQKMA
VAQAEAGAHIVSPSGMMDGQILAIREALDAAGFHDVAIMAYSAKYASAFYGPFRDAVGSSLQGDRRAYQQDPANLRESIL
EVDLDIEEGADFVMVKPAMPYLDVLAEVASTSSVPVAAYQVSGEYAMLHAAAANGWLDLDRVMMESLISIKRAGADQILT
YFAVEAARKL

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=311, Percent_Identity=43.4083601286174, Blast_Score=238, Evalue=5e-63,
Organism=Escherichia coli, GI87081728, Length=326, Percent_Identity=49.6932515337423, Blast_Score=306, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6321398, Length=314, Percent_Identity=39.8089171974522, Blast_Score=222, Evalue=7e-59,
Organism=Drosophila melanogaster, GI21358291, Length=299, Percent_Identity=41.4715719063545, Blast_Score=214, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36106; Mature: 36106

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDLIRRPRRLRSTPVMREFVAETTLRPADLILPMFIADGIDKPREIESMPGVYQHTME
CCCHHHCCCHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHH
SLIEAVREAISVGIRCVDLFGVPLDSDKDATGSQAWDPEGILNRGVRALRQEFGDQVLIM
HHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEE
ADTCLDEFTDHGHCGILTTDRFGATIVDNDPTVELYQKMAVAQAEAGAHIVSPSGMMDGQ
EHHHHHHHCCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCH
ILAIREALDAAGFHDVAIMAYSAKYASAFYGPFRDAVGSSLQGDRRAYQQDPANLRESIL
HHHHHHHHHHCCCCHHHHEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHH
EVDLDIEEGADFVMVKPAMPYLDVLAEVASTSSVPVAAYQVSGEYAMLHAAAANGWLDLD
HHCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEHHCCCCHHHH
RVMMESLISIKRAGADQILTYFAVEAARKL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLDLIRRPRRLRSTPVMREFVAETTLRPADLILPMFIADGIDKPREIESMPGVYQHTME
CCCHHHCCCHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHHH
SLIEAVREAISVGIRCVDLFGVPLDSDKDATGSQAWDPEGILNRGVRALRQEFGDQVLIM
HHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEE
ADTCLDEFTDHGHCGILTTDRFGATIVDNDPTVELYQKMAVAQAEAGAHIVSPSGMMDGQ
EHHHHHHHCCCCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCH
ILAIREALDAAGFHDVAIMAYSAKYASAFYGPFRDAVGSSLQGDRRAYQQDPANLRESIL
HHHHHHHHHHCCCCHHHHEEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHH
EVDLDIEEGADFVMVKPAMPYLDVLAEVASTSSVPVAAYQVSGEYAMLHAAAANGWLDLD
HHCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEHHCCCCHHHH
RVMMESLISIKRAGADQILTYFAVEAARKL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]