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The map label for this gene is hemC

Identifier: 38233015

GI number: 38233015

Start: 366113

End: 366997

Strand: Direct

Name: hemC

Synonym: DIP0401

Alternate gene names: 38233015

Gene position: 366113-366997 (Clockwise)

Preceding gene: 38233014

Following gene: 38233016

Centisome position: 14.71

GC content: 57.63

Gene sequence:

>885_bases
ATGACGTTGAAGATCGGCACCCGTGGAAGCCTGTTAGCTACCACGCAGTCGGGCCATGTTCGCGATGACCTGCGCCCCTA
TGAGGCAGAGCTGGTGATCGTGACTACTCATGGTGACGTGAACATGGCTCCGGTGGAGCGCATTGGTGTTGGCGTGTTTA
CCCAAGCCCTCCGCGAGGAGTTGCACACGGGTGGCTGCGATATTGCAGTGCATTCGTTTAAGGATCTTCCCACCGAGTTG
GATCCGCGGTTTCATTTGGTTGTGCCCAAGCGTGCCGATTTCCGTGATTGTTTGATCGCCCGTGATGGCTTGTCGTTGGC
TGATCTTCCTGCTGGTGCGTTGATTGGCACGGGTGCTCCGCGTCGTATTTCGCAGATCAAGGCGTTGCGCCCTGATGTTG
AGTGCGTGCCATTGCGCGGCAACATTGATACCCGTATGGGCAAGGTAACCAGTGGTGAGCTCGACGCTGTGGTGTTGGCT
TATGCCGGTCTTACCCGTGTGGGGCGTGGGGATGAGGCTACGCAGGTTTTTGATCCGCAGGACTTTTTGCCGGCACCAGC
TCAGGGTGCGTTGGCGGTGGAGTGCCGAGCAGATGATGCGTATGCCCGCGAGGCGATTGATTCTATCTGCGACGAGCAAG
CACAGGTGTGCGCTGTTACCGAGCGTGTAGTACTTAATCGCCTTGAGGCGGGATGCACGGCCCCGGTGGCTGCGCATGCA
ACGCTTAACGACGGCACGCTGACGTTGACTGCAGCAGTGATTGCTTTGGATGGTTCGAAGGTGGTTCGTCGTGAACTATC
TGCGCCAGTAGCGGAGAATGTGGCGTTGGCGGAGGAGCTTACGCGTGCTTTGATTGCTGATGGTGCTGCGACCATCTTGG
GATAA

Upstream 100 bases:

>100_bases
TGTCCTACGACACGGCCATTCAAGAGCTTTTTGGGCTTGCGGTCGAGGCGACACCTGTTGCTGTGAATGTGGATGAACTG
CCGGAAAGGATTAACAAGCG

Downstream 100 bases:

>100_bases
TTCCGTAACTTCAATTATGGTTATGGTTTTAGTTTCCTTCTATCGGCAGCACGCCTAGAAATGCGATTAATCATGAAGCC
CGAGCACTCAACGCGAGGAC

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTLKIGTRGSLLATTQSGHVRDDLRPYEAELVIVTTHGDVNMAPVERIGVGVFTQALREELHTGGCDIAVHSFKDLPTEL
DPRFHLVVPKRADFRDCLIARDGLSLADLPAGALIGTGAPRRISQIKALRPDVECVPLRGNIDTRMGKVTSGELDAVVLA
YAGLTRVGRGDEATQVFDPQDFLPAPAQGALAVECRADDAYAREAIDSICDEQAQVCAVTERVVLNRLEAGCTAPVAAHA
TLNDGTLTLTAAVIALDGSKVVRRELSAPVAENVALAEELTRALIADGAATILG

Sequences:

>Translated_294_residues
MTLKIGTRGSLLATTQSGHVRDDLRPYEAELVIVTTHGDVNMAPVERIGVGVFTQALREELHTGGCDIAVHSFKDLPTEL
DPRFHLVVPKRADFRDCLIARDGLSLADLPAGALIGTGAPRRISQIKALRPDVECVPLRGNIDTRMGKVTSGELDAVVLA
YAGLTRVGRGDEATQVFDPQDFLPAPAQGALAVECRADDAYAREAIDSICDEQAQVCAVTERVVLNRLEAGCTAPVAAHA
TLNDGTLTLTAAVIALDGSKVVRRELSAPVAENVALAEELTRALIADGAATILG
>Mature_293_residues
TLKIGTRGSLLATTQSGHVRDDLRPYEAELVIVTTHGDVNMAPVERIGVGVFTQALREELHTGGCDIAVHSFKDLPTELD
PRFHLVVPKRADFRDCLIARDGLSLADLPAGALIGTGAPRRISQIKALRPDVECVPLRGNIDTRMGKVTSGELDAVVLAY
AGLTRVGRGDEATQVFDPQDFLPAPAQGALAVECRADDAYAREAIDSICDEQAQVCAVTERVVLNRLEAGCTAPVAAHAT
LNDGTLTLTAAVIALDGSKVVRRELSAPVAENVALAEELTRALIADGAATILG

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=279, Percent_Identity=34.0501792114695, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI66933009, Length=279, Percent_Identity=34.0501792114695, Blast_Score=152, Evalue=4e-37,
Organism=Escherichia coli, GI48994974, Length=299, Percent_Identity=37.7926421404682, Blast_Score=179, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6319996, Length=315, Percent_Identity=34.9206349206349, Blast_Score=159, Evalue=4e-40,
Organism=Drosophila melanogaster, GI20130425, Length=292, Percent_Identity=36.3013698630137, Blast_Score=152, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_CORDI (Q6NJK0)

Other databases:

- EMBL:   BX248355
- RefSeq:   NP_938782.1
- ProteinModelPortal:   Q6NJK0
- SMR:   Q6NJK0
- GeneID:   2650205
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0401
- NMPDR:   fig|257309.1.peg.373
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- PhylomeDB:   Q6NJK0
- ProtClustDB:   PRK00072
- BioCyc:   CDIP257309:DIP0401-MONOMER
- BRENDA:   2.5.1.61
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 31074; Mature: 30943

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKIGTRGSLLATTQSGHVRDDLRPYEAELVIVTTHGDVNMAPVERIGVGVFTQALREE
CEEEECCCCCEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LHTGGCDIAVHSFKDLPTELDPRFHLVVPKRADFRDCLIARDGLSLADLPAGALIGTGAP
HHCCCCEEEEECHHCCCCCCCCCEEEEECCCCCHHHHHEECCCCCHHCCCCCCEECCCCC
RRISQIKALRPDVECVPLRGNIDTRMGKVTSGELDAVVLAYAGLTRVGRGDEATQVFDPQ
HHHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCH
DFLPAPAQGALAVECRADDAYAREAIDSICDEQAQVCAVTERVVLNRLEAGCTAPVAAHA
HCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
TLNDGTLTLTAAVIALDGSKVVRRELSAPVAENVALAEELTRALIADGAATILG
EECCCEEEEEEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHCC
>Mature Secondary Structure 
TLKIGTRGSLLATTQSGHVRDDLRPYEAELVIVTTHGDVNMAPVERIGVGVFTQALREE
EEEECCCCCEEEECCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LHTGGCDIAVHSFKDLPTELDPRFHLVVPKRADFRDCLIARDGLSLADLPAGALIGTGAP
HHCCCCEEEEECHHCCCCCCCCCEEEEECCCCCHHHHHEECCCCCHHCCCCCCEECCCCC
RRISQIKALRPDVECVPLRGNIDTRMGKVTSGELDAVVLAYAGLTRVGRGDEATQVFDPQ
HHHHHHHHCCCCCEEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCH
DFLPAPAQGALAVECRADDAYAREAIDSICDEQAQVCAVTERVVLNRLEAGCTAPVAAHA
HCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
TLNDGTLTLTAAVIALDGSKVVRRELSAPVAENVALAEELTRALIADGAATILG
EECCCEEEEEEEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910