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The map label for this gene is murB

Identifier: 38232998

GI number: 38232998

Start: 350143

End: 351276

Strand: Direct

Name: murB

Synonym: DIP0384

Alternate gene names: 38232998

Gene position: 350143-351276 (Clockwise)

Preceding gene: 38232993

Following gene: 38233002

Centisome position: 14.07

GC content: 59.08

Gene sequence:

>1134_bases
TTGTCATGCATACAGACTACCTTTAATGAGGTGCTGGAAAAATCACTGAACCACATTTTAGACGGAATCCGCCCCCAGAT
CGAGGCGATCGACGACGCCGCCTTTGTGGCGGTCACCTTCGCCGAATTGACCACCCTCCACCTCGGCGGAACCCCCATGG
CGGCGGTTCGCTGCAGGTCACAGCATTCTGTGGTTGAGGTTGTTCGGCTTCTCGACGCCCACCAGATCCCACTACTCATC
GTAGGTGGTGGCTCCAACCTCGTGATCGCTGACGGCGAAATACCCCTCGTTGCTGTGATTCTCGACTGTGACGATATTTC
TGTGACCCTCGACACCGGACGCGTAGTAGCCGAAGCAGGTGCCGTATGGGACGACGTCGTACGCCTGTGCGTTGATGCCG
GTCTCGGCGGCATTGAGTGCCTTTCTGGAATTCCTGGCTCGGCGGGAGCTACCCCTGTACAAAACGTGGGCGCCTACGGT
GCAGAGATTTCCGATGTGTTGGTCAGCGTAACCCTCTTGGAACGTGCAACAGGGGCGGTTATGGAAGTTCCTGCGGCCGA
CTTAGAGCTGGCGTATCGCTATTCCAACCTCAAATTCACCGGCCGTGGCGTGGTACTGGGCATTACGCTACAGCTGCACA
CGGATGGCATGTCTGCGCCGCTGCGCTTTGGGGAGCTTGCTCGGGTGCTAGGCCATGAGGGACCACATCCTGCAGTGCAG
GTGCGCGAGGCTGTACTTGGCCTGCGTGCTGGCAAGGGCATGGTCTACAACGAGGCGGATCACGACACGTGGTCGGCCGG
ATCCTTCTTTACCAACCCCATCGTCCCAGAATCGGTCGGGGATCACGTACGTAGCGTTGTCGGCGACGAGTCGATGCCAT
GCTTTGCTGCGGGCGAGGGCATGGTCAAACTATCTGCTGCATGGTTGATTGACCGCGCCGGATTTGCCAAAGGCCACCAA
GGCCCTGGCGGGCGGGTGAGCTTGTCTACTAAGCACACGCTGGCACTGACCAATAGAGGAAACGCCACCACGGATGATCT
CGTCGCACTTGCGCGCGAGGTCCGTGGCGGCGTGATGGATGCCTTCGGGGTGCTACTTGAGCCCGAACCGGTATGGGTTG
GGGTGTCTATTTAA

Upstream 100 bases:

>100_bases
CTGAGGTTTTTCACAATGAAGTCCCAATATGCCGGATTGGTCAAGGCTGCGTGCACTTTTTCCACTGGCTGATTGATAGT
TACGGTGTTTTCACTTCGCG

Downstream 100 bases:

>100_bases
CCCAAGATGCACTGAGCAAGAAGCATGTCGAGGCGTCGTACGTCGAGATCTCGGCCGATTTTGATCTTGATTTTGCCGGC
ACGCGTCCACAACTCCACTT

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSCIQTTFNEVLEKSLNHILDGIRPQIEAIDDAAFVAVTFAELTTLHLGGTPMAAVRCRSQHSVVEVVRLLDAHQIPLLI
VGGGSNLVIADGEIPLVAVILDCDDISVTLDTGRVVAEAGAVWDDVVRLCVDAGLGGIECLSGIPGSAGATPVQNVGAYG
AEISDVLVSVTLLERATGAVMEVPAADLELAYRYSNLKFTGRGVVLGITLQLHTDGMSAPLRFGELARVLGHEGPHPAVQ
VREAVLGLRAGKGMVYNEADHDTWSAGSFFTNPIVPESVGDHVRSVVGDESMPCFAAGEGMVKLSAAWLIDRAGFAKGHQ
GPGGRVSLSTKHTLALTNRGNATTDDLVALAREVRGGVMDAFGVLLEPEPVWVGVSI

Sequences:

>Translated_377_residues
MSCIQTTFNEVLEKSLNHILDGIRPQIEAIDDAAFVAVTFAELTTLHLGGTPMAAVRCRSQHSVVEVVRLLDAHQIPLLI
VGGGSNLVIADGEIPLVAVILDCDDISVTLDTGRVVAEAGAVWDDVVRLCVDAGLGGIECLSGIPGSAGATPVQNVGAYG
AEISDVLVSVTLLERATGAVMEVPAADLELAYRYSNLKFTGRGVVLGITLQLHTDGMSAPLRFGELARVLGHEGPHPAVQ
VREAVLGLRAGKGMVYNEADHDTWSAGSFFTNPIVPESVGDHVRSVVGDESMPCFAAGEGMVKLSAAWLIDRAGFAKGHQ
GPGGRVSLSTKHTLALTNRGNATTDDLVALAREVRGGVMDAFGVLLEPEPVWVGVSI
>Mature_376_residues
SCIQTTFNEVLEKSLNHILDGIRPQIEAIDDAAFVAVTFAELTTLHLGGTPMAAVRCRSQHSVVEVVRLLDAHQIPLLIV
GGGSNLVIADGEIPLVAVILDCDDISVTLDTGRVVAEAGAVWDDVVRLCVDAGLGGIECLSGIPGSAGATPVQNVGAYGA
EISDVLVSVTLLERATGAVMEVPAADLELAYRYSNLKFTGRGVVLGITLQLHTDGMSAPLRFGELARVLGHEGPHPAVQV
REAVLGLRAGKGMVYNEADHDTWSAGSFFTNPIVPESVGDHVRSVVGDESMPCFAAGEGMVKLSAAWLIDRAGFAKGHQG
PGGRVSLSTKHTLALTNRGNATTDDLVALAREVRGGVMDAFGVLLEPEPVWVGVSI

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=317, Percent_Identity=33.1230283911672, Blast_Score=128, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_CORDI (P61434)

Other databases:

- EMBL:   BX248355
- RefSeq:   NP_938765.1
- ProteinModelPortal:   P61434
- SMR:   P61434
- GeneID:   2648803
- GenomeReviews:   BX248353_GR
- KEGG:   cdi:DIP0384
- NMPDR:   fig|257309.1.peg.356
- HOGENOM:   HBG686573
- OMA:   GAAPMQN
- PhylomeDB:   P61434
- ProtClustDB:   PRK13903
- BioCyc:   CDIP257309:DIP0384-MONOMER
- BRENDA:   1.1.1.158
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 39437; Mature: 39305

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 193-193 ACT_SITE 268-268 ACT_SITE 369-369

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSCIQTTFNEVLEKSLNHILDGIRPQIEAIDDAAFVAVTFAELTTLHLGGTPMAAVRCRS
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHEEEEEHHHHHEEEECCCCHHHHHCCC
QHSVVEVVRLLDAHQIPLLIVGGGSNLVIADGEIPLVAVILDCDDISVTLDTGRVVAEAG
CHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCEEEEEEECCCEEEEEECCCEEHHCC
AVWDDVVRLCVDAGLGGIECLSGIPGSAGATPVQNVGAYGAEISDVLVSVTLLERATGAV
CHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCE
MEVPAADLELAYRYSNLKFTGRGVVLGITLQLHTDGMSAPLRFGELARVLGHEGPHPAVQ
EECCCCCEEEEEEECCEEEECCEEEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHH
VREAVLGLRAGKGMVYNEADHDTWSAGSFFTNPIVPESVGDHVRSVVGDESMPCFAAGEG
HHHHHHHHHCCCCCEEECCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCCCEEECCCC
MVKLSAAWLIDRAGFAKGHQGPGGRVSLSTKHTLALTNRGNATTDDLVALAREVRGGVMD
EEEEEHHHHHCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCHHHHHHHHHHHCCCHHH
AFGVLLEPEPVWVGVSI
HHHHEECCCCEEEEECC
>Mature Secondary Structure 
SCIQTTFNEVLEKSLNHILDGIRPQIEAIDDAAFVAVTFAELTTLHLGGTPMAAVRCRS
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHEEEEEHHHHHEEEECCCCHHHHHCCC
QHSVVEVVRLLDAHQIPLLIVGGGSNLVIADGEIPLVAVILDCDDISVTLDTGRVVAEAG
CHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCEEEEEEECCCEEEEEECCCEEHHCC
AVWDDVVRLCVDAGLGGIECLSGIPGSAGATPVQNVGAYGAEISDVLVSVTLLERATGAV
CHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCE
MEVPAADLELAYRYSNLKFTGRGVVLGITLQLHTDGMSAPLRFGELARVLGHEGPHPAVQ
EECCCCCEEEEEEECCEEEECCEEEEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHH
VREAVLGLRAGKGMVYNEADHDTWSAGSFFTNPIVPESVGDHVRSVVGDESMPCFAAGEG
HHHHHHHHHCCCCCEEECCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCCCEEECCCC
MVKLSAAWLIDRAGFAKGHQGPGGRVSLSTKHTLALTNRGNATTDDLVALAREVRGGVMD
EEEEEHHHHHCCCCCCCCCCCCCCEEEEECCEEEEEECCCCCCHHHHHHHHHHHCCCHHH
AFGVLLEPEPVWVGVSI
HHHHEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14602910