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The map label for this gene is dppA [H]

Identifier: 38232945

GI number: 38232945

Start: 288591

End: 290078

Strand: Direct

Name: dppA [H]

Synonym: DIP0324

Alternate gene names: 38232945

Gene position: 288591-290078 (Clockwise)

Preceding gene: 38232944

Following gene: 38232946

Centisome position: 11.6

GC content: 60.62

Gene sequence:

>1488_bases
ATGAAGAAAAAACTCGGCCTCGCGGTAGCAACCCTCGCCGTTGCTACCACATTGAGCGGCTGCTTCACCGACGCTGGGCA
GGCTAACGACGACGCCCTGCGCGTGGCACTACAGTTCAAGCCCGTCGCAGACTTCTCGCCATACTCCGACGACGCAGTGC
TCAACCTGCGCATGGGGGCCGTGGAAACCCTTGTCACCCTAGACGAGGATGGCAAACCCAAACCCGTTCTTGCCGAGAAA
TGGGAAATGAAAGACGACCGCACCGCAGTACTGACCCTGCGCAAGGACGTTAGCTTCCACGACGGCTCCAAACTCACCGG
CGAGGTAGTAAAAAACGCCCTCGACCATGCGCTTTCTGCCACAACTCGCCCCAAAGGCTTGGGCAAAGCCGACCTGAAGG
TCGAAGCCACCGGCGAGTCCGAGGTGACCATCACCGCACCAGAAGCCGACCCGATCCTCGTGCAGCGCCTCACCGACCCA
GGTGCCGCCATCTTGTCCAAGGACGCCTACGCTGGTGAAAACCCAGACCCGTTTAACCACGGCACCGGCCCCTTCAAGCT
AGTGAAGAAGGAATCCGATGGTTCTGTTAGCGCCGAGGCATTTGCCGACTACTGGAACGGGACCCCAGAAACCAGCGCAC
TGAAGGTGTCCTTCATCGAAGACGGCGCCGCCCGCGCCAACGCCTTCCGCGCAGGGGACTTCGACGTGATCAAGGGCGTG
CCAGTGGTTGCGCTACCTGAGCTTTCCGACGCCCACGTCACCGATGTTCACCTGCCACGCGCGACATTGCTGCACCTCAA
CGCCGAAAAGGGCGTGTTCGCGGATGCCAAGCTGCGTACCGCAGTTGCTGGGGCTATCAAGACAGATGCGATCGTGGACA
AGATCTACGAGGGCAAGGCCAACAAGACCGAGGGCTCCCTGTTTAACCTTGATGCGCAGTGGGCTCAGAAGGCAAAACCG
CTTGCCGGCGCACCTGCTGACGCCACCATCGGCAAGGGCAAGACCGTTCGCCTTGCCACCTGGGACTCCCGCGCTGAGCT
GCCGGAAACCGCGAACCTCATCGCGGACCAGCTGCGCGCCTTGGGCTTTAACGTCGACATCACGGTCGCCGATTACGCCT
CCCTAGAAAACCGGCTTCTCGACGGCTCCTTCGACGCCGTGATCGGTTCCCGCAGCTACATGATCGGTGCTGGCGACCCC
GTTGCCTTCCTAGATACCGACTTCACCTGTGACGGCAGCTACAACCTGTCGCGCCTGTGCGACCCAGAGATCGACAAGAA
GATTGCCGCAGCCAAGAGCGAGAAAGACCTCGATACGCGGCACAAGGATGCCGCAGCAATTGGCGCAGACGTGGTAGCCA
CCGGTTCCGTAGTGCCACTCGCACACGAGCAGCTGCTTGTGGTGTCCAAGGACGTGGAAGGCATTGCTGCCGACCCAATG
GAACGCTCCCTGATCACCGAAAAGACCAAGAAGACCGCTAAGAAGTAA

Upstream 100 bases:

>100_bases
CGATGGCCGCCATCCCGTGGCTCATCATGGTTGCAGTACTCGCCGTCTCCGCAGTGGGACTGGCACAACTGAAAGAAAAA
CAGAAAGTAGACATCACACA

Downstream 100 bases:

>100_bases
ATGACCATGAGATCGCCTCGCACCCTCGCCACGGTTATTATCGCTGTGGCAACCTATGCGCTCGCTTTGGTAGCCACCGC
ACTATTGCCCTGGTTTACCG

Product: hypothetical protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MKKKLGLAVATLAVATTLSGCFTDAGQANDDALRVALQFKPVADFSPYSDDAVLNLRMGAVETLVTLDEDGKPKPVLAEK
WEMKDDRTAVLTLRKDVSFHDGSKLTGEVVKNALDHALSATTRPKGLGKADLKVEATGESEVTITAPEADPILVQRLTDP
GAAILSKDAYAGENPDPFNHGTGPFKLVKKESDGSVSAEAFADYWNGTPETSALKVSFIEDGAARANAFRAGDFDVIKGV
PVVALPELSDAHVTDVHLPRATLLHLNAEKGVFADAKLRTAVAGAIKTDAIVDKIYEGKANKTEGSLFNLDAQWAQKAKP
LAGAPADATIGKGKTVRLATWDSRAELPETANLIADQLRALGFNVDITVADYASLENRLLDGSFDAVIGSRSYMIGAGDP
VAFLDTDFTCDGSYNLSRLCDPEIDKKIAAAKSEKDLDTRHKDAAAIGADVVATGSVVPLAHEQLLVVSKDVEGIAADPM
ERSLITEKTKKTAKK

Sequences:

>Translated_495_residues
MKKKLGLAVATLAVATTLSGCFTDAGQANDDALRVALQFKPVADFSPYSDDAVLNLRMGAVETLVTLDEDGKPKPVLAEK
WEMKDDRTAVLTLRKDVSFHDGSKLTGEVVKNALDHALSATTRPKGLGKADLKVEATGESEVTITAPEADPILVQRLTDP
GAAILSKDAYAGENPDPFNHGTGPFKLVKKESDGSVSAEAFADYWNGTPETSALKVSFIEDGAARANAFRAGDFDVIKGV
PVVALPELSDAHVTDVHLPRATLLHLNAEKGVFADAKLRTAVAGAIKTDAIVDKIYEGKANKTEGSLFNLDAQWAQKAKP
LAGAPADATIGKGKTVRLATWDSRAELPETANLIADQLRALGFNVDITVADYASLENRLLDGSFDAVIGSRSYMIGAGDP
VAFLDTDFTCDGSYNLSRLCDPEIDKKIAAAKSEKDLDTRHKDAAAIGADVVATGSVVPLAHEQLLVVSKDVEGIAADPM
ERSLITEKTKKTAKK
>Mature_495_residues
MKKKLGLAVATLAVATTLSGCFTDAGQANDDALRVALQFKPVADFSPYSDDAVLNLRMGAVETLVTLDEDGKPKPVLAEK
WEMKDDRTAVLTLRKDVSFHDGSKLTGEVVKNALDHALSATTRPKGLGKADLKVEATGESEVTITAPEADPILVQRLTDP
GAAILSKDAYAGENPDPFNHGTGPFKLVKKESDGSVSAEAFADYWNGTPETSALKVSFIEDGAARANAFRAGDFDVIKGV
PVVALPELSDAHVTDVHLPRATLLHLNAEKGVFADAKLRTAVAGAIKTDAIVDKIYEGKANKTEGSLFNLDAQWAQKAKP
LAGAPADATIGKGKTVRLATWDSRAELPETANLIADQLRALGFNVDITVADYASLENRLLDGSFDAVIGSRSYMIGAGDP
VAFLDTDFTCDGSYNLSRLCDPEIDKKIAAAKSEKDLDTRHKDAAAIGADVVATGSVVPLAHEQLLVVSKDVEGIAADPM
ERSLITEKTKKTAKK

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=537, Percent_Identity=24.9534450651769, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787762, Length=436, Percent_Identity=24.0825688073394, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789887, Length=484, Percent_Identity=26.2396694214876, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1787052, Length=443, Percent_Identity=23.0248306997743, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 52467; Mature: 52467

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLGLAVATLAVATTLSGCFTDAGQANDDALRVALQFKPVADFSPYSDDAVLNLRMGA
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEEECCC
VETLVTLDEDGKPKPVLAEKWEMKDDRTAVLTLRKDVSFHDGSKLTGEVVKNALDHALSA
EEEEEEECCCCCCCCCCHHCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHC
TTRPKGLGKADLKVEATGESEVTITAPEADPILVQRLTDPGAAILSKDAYAGENPDPFNH
CCCCCCCCCCCEEEEECCCCEEEEECCCCCCEEEEEECCCCHHHEECCCCCCCCCCCCCC
GTGPFKLVKKESDGSVSAEAFADYWNGTPETSALKVSFIEDGAARANAFRAGDFDVIKGV
CCCCEEEEEECCCCCEEHHHHHHHCCCCCCCCEEEEEEECCCCHHCCCCCCCCCHHHCCC
PVVALPELSDAHVTDVHLPRATLLHLNAEKGVFADAKLRTAVAGAIKTDAIVDKIYEGKA
CEEEECCCCCCCEEEEECCCEEEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCC
NKTEGSLFNLDAQWAQKAKPLAGAPADATIGKGKTVRLATWDSRAELPETANLIADQLRA
CCCCCCEEECCHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
LGFNVDITVADYASLENRLLDGSFDAVIGSRSYMIGAGDPVAFLDTDFTCDGSYNLSRLC
CCCCEEEEEHHHHHHHHHHCCCCHHHEECCCEEEEECCCCEEEEECCEEECCCCCHHHCC
DPEIDKKIAAAKSEKDLDTRHKDAAAIGADVVATGSVVPLAHEQLLVVSKDVEGIAADPM
CCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEECCCEEECCCCEEEEEECCCCCCCCCCH
ERSLITEKTKKTAKK
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKKLGLAVATLAVATTLSGCFTDAGQANDDALRVALQFKPVADFSPYSDDAVLNLRMGA
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCCCEEEEEECCC
VETLVTLDEDGKPKPVLAEKWEMKDDRTAVLTLRKDVSFHDGSKLTGEVVKNALDHALSA
EEEEEEECCCCCCCCCCHHCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHC
TTRPKGLGKADLKVEATGESEVTITAPEADPILVQRLTDPGAAILSKDAYAGENPDPFNH
CCCCCCCCCCCEEEEECCCCEEEEECCCCCCEEEEEECCCCHHHEECCCCCCCCCCCCCC
GTGPFKLVKKESDGSVSAEAFADYWNGTPETSALKVSFIEDGAARANAFRAGDFDVIKGV
CCCCEEEEEECCCCCEEHHHHHHHCCCCCCCCEEEEEEECCCCHHCCCCCCCCCHHHCCC
PVVALPELSDAHVTDVHLPRATLLHLNAEKGVFADAKLRTAVAGAIKTDAIVDKIYEGKA
CEEEECCCCCCCEEEEECCCEEEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHCCCC
NKTEGSLFNLDAQWAQKAKPLAGAPADATIGKGKTVRLATWDSRAELPETANLIADQLRA
CCCCCCEEECCHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
LGFNVDITVADYASLENRLLDGSFDAVIGSRSYMIGAGDPVAFLDTDFTCDGSYNLSRLC
CCCCEEEEEHHHHHHHHHHCCCCHHHEECCCEEEEECCCCEEEEECCEEECCCCCHHHCC
DPEIDKKIAAAKSEKDLDTRHKDAAAIGADVVATGSVVPLAHEQLLVVSKDVEGIAADPM
CCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEECCCEEECCCCEEEEEECCCCCCCCCCH
ERSLITEKTKKTAKK
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]